Antibacterial ceramic was prepared by doping enamel slurry with composite phosphate inorganic antibacterial materials containing rare earth (inorganic antibacterial additives), and then the mechanisms for activating w...Antibacterial ceramic was prepared by doping enamel slurry with composite phosphate inorganic antibacterial materials containing rare earth (inorganic antibacterial additives), and then the mechanisms for activating water and improving seed germinative property were tested by nuclear magnetic resonance (NMR) and the method of testing oxygen dissolved in activated water. Results show that the half peak width of (()^(17)O-NMR) for tap water activated by the antibacterial ceramic drops from 115.36 to 99.15 Hz, and oxygen concentrations of activated water increase by 20%, germinate rate of horsebean and earthnut seeds increases by 12.5% and 7.5%, respectively. Therefore antibacterial ceramic doped enamel slurry with inorganic antibacterial additives containing rare earth can reduce the volume of clusters of water molecules, improve activation of tap water, and promote plant seeds germinate.展开更多
Fracture surface and surface of two kind of films of antibacterial bone china with 3% and 5% composite phosphate containing rare earth were characterized by SEM and nanoindenter, respectively, and the hardness and ela...Fracture surface and surface of two kind of films of antibacterial bone china with 3% and 5% composite phosphate containing rare earth were characterized by SEM and nanoindenter, respectively, and the hardness and elastic modulus were measured by nanoindenter at different load conditions. The results show that hardness and elastic modulus of the two kinds of films have obvious change. Elastic modulus versus maximal depth of indentation curves are almost linear. Hardness versus maximal depth of indentation of film with 5% rare earth content curve is nearly linear, while that of 3% increases at first, then decreases. Obvious pile-up of indentation impression of ceramic film with 3% rare earth content is observed with h_f/h_ max<0.7 and different loads, however indentation impression of 5% has no this kind of phenomenon. Special properties of two kinds of ceramic films are because that films are the mixture of crystal and non-crystal materials.展开更多
Orthopedic infections pose severe societal and economic burden and interfere with the capability of the implanted devices to integrate in the host bone,thus significantly increasing implants failure rate.To address in...Orthopedic infections pose severe societal and economic burden and interfere with the capability of the implanted devices to integrate in the host bone,thus significantly increasing implants failure rate.To address infection and promote integration,here nanostructured antibacterial and bioactive thin films are proposed,obtained,for the first time,by Ionized Jet Deposition(IJD)of silver-substituted tricalcium phosphate(Ag-TCP)targets on titanium.Coatings morphology,composition and mechanical properties are characterized and proof-of-concept of biocompatibility is shown.Antimicrobial efficacy is investigated against four Gram positive and Gram negative bacterial strains and against C.albicans fungus,by investigating the modifications in planktonic bacterial growth in the absence and presence of silver.Then,for all bacterial strains,the capability of the film to inhibit bacterial adhesion is also tested.Results indicate that IJD permits a fine control over films composition and morphology and deposition of films with suitable mechanical properties.Biological studies show a good efficacy against Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa,Enterococcus faecalis and against fungus Candida albicans,with evidences of efficacy against planktonic growth and significant reduction of bacterial cell adhesion.No cytotoxic effects are evidenced for equine adipose tissue derived mesenchymal stem cells(ADMSCs),as no reductions are caused to cells viability and no interference is assessed in cells differentiation towards osteogenic lineage,in the presence of silver.Instead,thanks to nanostructuration and biomimetic composition,tricalcium phosphate(TCP)coatings favor cells viability,also when silver-substituted.These findings show that silver-substituted nanostructured coatings are promising for orthopedic implant applications.展开更多
文摘Antibacterial ceramic was prepared by doping enamel slurry with composite phosphate inorganic antibacterial materials containing rare earth (inorganic antibacterial additives), and then the mechanisms for activating water and improving seed germinative property were tested by nuclear magnetic resonance (NMR) and the method of testing oxygen dissolved in activated water. Results show that the half peak width of (()^(17)O-NMR) for tap water activated by the antibacterial ceramic drops from 115.36 to 99.15 Hz, and oxygen concentrations of activated water increase by 20%, germinate rate of horsebean and earthnut seeds increases by 12.5% and 7.5%, respectively. Therefore antibacterial ceramic doped enamel slurry with inorganic antibacterial additives containing rare earth can reduce the volume of clusters of water molecules, improve activation of tap water, and promote plant seeds germinate.
文摘Fracture surface and surface of two kind of films of antibacterial bone china with 3% and 5% composite phosphate containing rare earth were characterized by SEM and nanoindenter, respectively, and the hardness and elastic modulus were measured by nanoindenter at different load conditions. The results show that hardness and elastic modulus of the two kinds of films have obvious change. Elastic modulus versus maximal depth of indentation curves are almost linear. Hardness versus maximal depth of indentation of film with 5% rare earth content curve is nearly linear, while that of 3% increases at first, then decreases. Obvious pile-up of indentation impression of ceramic film with 3% rare earth content is observed with h_f/h_ max<0.7 and different loads, however indentation impression of 5% has no this kind of phenomenon. Special properties of two kinds of ceramic films are because that films are the mixture of crystal and non-crystal materials.
基金Dr.Gabriela Graziani acknowledges funding from the project Starting Grant SG-2018-12367059financed by the Italian Ministry of Health(BANDO RICERCA FINALIZZATA 2018).
文摘Orthopedic infections pose severe societal and economic burden and interfere with the capability of the implanted devices to integrate in the host bone,thus significantly increasing implants failure rate.To address infection and promote integration,here nanostructured antibacterial and bioactive thin films are proposed,obtained,for the first time,by Ionized Jet Deposition(IJD)of silver-substituted tricalcium phosphate(Ag-TCP)targets on titanium.Coatings morphology,composition and mechanical properties are characterized and proof-of-concept of biocompatibility is shown.Antimicrobial efficacy is investigated against four Gram positive and Gram negative bacterial strains and against C.albicans fungus,by investigating the modifications in planktonic bacterial growth in the absence and presence of silver.Then,for all bacterial strains,the capability of the film to inhibit bacterial adhesion is also tested.Results indicate that IJD permits a fine control over films composition and morphology and deposition of films with suitable mechanical properties.Biological studies show a good efficacy against Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa,Enterococcus faecalis and against fungus Candida albicans,with evidences of efficacy against planktonic growth and significant reduction of bacterial cell adhesion.No cytotoxic effects are evidenced for equine adipose tissue derived mesenchymal stem cells(ADMSCs),as no reductions are caused to cells viability and no interference is assessed in cells differentiation towards osteogenic lineage,in the presence of silver.Instead,thanks to nanostructuration and biomimetic composition,tricalcium phosphate(TCP)coatings favor cells viability,also when silver-substituted.These findings show that silver-substituted nanostructured coatings are promising for orthopedic implant applications.
