Synergistic photothermal effect and nanoenzyme for efficient antibacterial activity

The continuous emergence of drug-resistant bacteria has become a serious threat to human and animal health.Developing new non-antibiotic therapeutic drugs with high efficiency and low resistance is a promising strategy for combating drug-resistant bacteria.Here,a novel composite structure based on a hollow metal-organic framework(MOF)wrapped Au nanorod and loaded Pt nanoparticles(Au NR@H-ZIF-8@Pt)is designed for combating drug-resistant bacteria.The Au NR@H-ZIF-8@Pt composite structures serve a twofold purpose:(1)showing superior photothermal conversion efficiency under near-infrared light irradiation,thus achieving an antibacterial effect based on photothermal effect,and(2)exhibiting high-efficiency peroxidase-like activity,and effectively killing bacteria by reactive oxygen species(ROS)generated in situ.By combining photothermal and catalytic effects for in vitro antibacterial experiments,Au NR@H-ZIF-8@Pt exhibits efficient,rapid,broad-spectrum antibacterial activity(99.7%for Pseudomonas aeruginosa and 98.3%for Staphylococcus aureus).In addition,in vivo experiments further demonstrate the advantages of the material in promoting bacterial infection wound healing.This study not only facilitates the design of novel biological nanomaterials,but also provides new ideas for combating drug-resistant bacteria.

Copper-based nanodots as efficient biomimetic antibiotics for the treatment of oral bacterial infections Author links open overlay panel

Oral infectious diseases caused by a variety of pathogenic bacteria seriously affect the quality of life.However,these diseases remain a clinical challenge because of the lack of simple,safe,and universal prophylactics.To address these limitations,we synthesize CuO_(x)nanodots(CuO_(x)NDs)with excellent Fenton-like reaction activity and utilize them in the treatment of oral bacterial infections.Different from other complicated approaches,CuO_(x)NDs are rationally prepared using a facile one-pot aqueous synthesis.In the presence of H_(2)O_(2),these well-developed CuO_(x)NDs can efficiently catalyze the generation of hydroxyl radicals(·OH)around oral pathogens,leading to the death of various bacteria.Meanwhile,results of biosafety indicate the high biocompatibility and extremely low toxicity of these CuO_(x)NDs.After understanding the admirable in vitro antibacterial effect of CuO_(x)NDs in the presence of H_(2)O_(2),we further explore their in vivo antibacterial performance on several classical animal models including oral mucosal wound model,intragingival bacteria-infected model,and the periodontal infection model.As expected,these CuO_(x)NDs with wide-spectrum antibacterial activity can serve as high-performance antibacterial reagents for the treatment of various oral bacterial infections with the help of H_(2)O_(2).In brief,current nanoplatform can act as efficient antibiotics against oral pathogens with broadening the biomedical applications of copper-based nanomaterials.

Perioperative changes in lower gut microbiocenosis in patients with colon and rectum cancer

This clinical study was designed to determinate the value of colon microbiota microflora in colorectal cancer patients.We investigate pre-and post-operation stool samples in patients with different tumor localizations,trying to correlate those to postoperative complications.Our findings shows that if opportunistic pathogenic microflora levels are elevated before operations,it can lead to further microbiota disbalance in postoperative period,especially in cases of antibacterial prophylaxis or treatment.Further,this changes increase a chance of receiving some complications after surgery.So,we suggest an analysis of stool microbiota in patients with colorectal cancer to be performed prior and after the operation.Furthermore,aperioperative decontamination therapy should be performed in cases of pathogenic microflora levels elevation.Still,monitoring aperioperative microbiota changes is not a routine in most recommendations and we assume that including this relatively unencumbered patient testing in preoperative survey will help to reduce a number of postoperative complications.

Cervical spine infection arising from chronic paronychia:A case report and review of literature

BACKGROUND Cervical spine pyogenic infection(CSPI)is a rare and challenging form of spinal infection that is typically caused by pyogenic bacteria and primarily affects the cervical vertebral bodies and surrounding tissues.Given its nonspecific symptoms,such as fever and neck pain,early diagnosis is crucial to prevent severe complications,including spinal cord injury.We report a previously unreported case of acute CSPI arising from chronic paronychia,exploring its diagnostic and therapeutic challenges through a review of the current literature.CASE SUMMARY The presented case involved a 15-year-old man with CSPI caused by Staphylococcus aureus,which led to complications including bacteremia and a paronychiaassociated abscess.Acute pyogenic infection was initially diagnosed by typical symptoms and blood culture.Fever improved after antibiotic treatment while developing progressive limbs dysfunction.Six days after admission,the patient underwent anterior cervical debridement+autogenous iliac bone graft fusion+plate internal fixation and received 12 weeks of antibiotic treatment after the operation.Re-examination 3 years postoperatively showed that the patient had stable cervical fixation,no significant neck pain or upper limb abnormalities,and normal urinary function.CONCLUSION Early imaging findings,laboratory markers,and timely antibiotic treatment are crucial for CSPI management,preventing complications and facilitating recovery.

Virus-inspired nanoparticles as versatile antibacterial carriers for antibiotic delivery against Gram-negative and Gram-positive bacteria

Infectious diseases become one of the leading causes of human death. Traditional treatment based on classical antibiotics could not provide enough antibacterial activity to combat bacterial infections due to low bioavailability, even leading to antibiotic resistance. In recent years, biomimetic delivery systems have been developed to improve drug therapy for various diseases, such as malignant tumor and cardiovascular disease. In this work, we designed virus-inspired nanodrugs(VNDs) through co-assembly of amphiphilic lipopeptide dendrons and poly(lactic-co-glycolic acid) polymers for high-efficiency antibiotic delivery. These VNDs had well-defined and stable nanostructures for tetracycline encapsulation and delivery. The VNDs were capable of promoting antibiotic internalization and enhancing their antibacterial effects against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. Additionally, no obvious cytotoxicity of VNDs was observed to human cell lines. This work successfully demonstrated the virus-mimetic nanoparticles served as promising and applicable antibiotic delivery platform for antibacterial treatment.

Ion-π interactions for constructing organic luminescent materials

Organic luminescent materials are one of the research hotspots worldwide.The luminescent efficiency of these materials can be tuned by introducing noncovalent interactions.Ion-π interactions,mainly including anion-π interactions and cation-π interactions,are relatively new but powerful noncovalent interactions with extensive applications in supramolecular chemistry.The use of ion-π interactions to fabricate highly luminescent materials is initiated since they were introduced to develop aggregation-induced emission luminogens in 2017.Then,this research field is undergoing through challenging but very prospective developments.In this minireview,we start with a brief introduction of ion-π interactions and then make a summary of the reports on organic fluorescence based on ion-π interactions and room temperature phosphorescence induced by ion-π interactions.The applications of organic luminescent materials based on ion-π interactions in bioimaging,imaging-guided anticancer and antibacterial treatment are also discussed.The challenges and prospects of using anion-π and cation-π interactions for constructing organic luminogens are presented in the final part.It is expected that this minireview will provide some guidelines for fabricating novel organic luminescent materials and further extend the application potential of ion-π interactions.