[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides (mytilin and myticin) from Mytilus coruscus.[Method] By the screening of antibacterial pept...[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides (mytilin and myticin) from Mytilus coruscus.[Method] By the screening of antibacterial peptide genes of mytilin and myticin of Mytilus coruscus,five antibacterial peptide genes were selected.Then,the relative eukaryotic expressing vectors were constructed by the use of PCR technique and DNA recombinant technology.Subsequently,they were transferred in to S78 Saccharomyces cerevisia by using LiAC transformation method,and then preliminary expressing analysis was carried out.[Result] Five eukaryotic expressing vectors of antibacterial peptides from Mytilus coruscus were successfully constructed,and the results of mRNA detection revealed that the five antibacterial peptides from Mytilus coruscus were successfully transcribed.[Conclusion] The results provide a basis for using genetic engineering to express antibacterial peptides of mytilin and myticin from Mytilus coruscus,and for developing the further study of antibacterial peptides from Mytilus coruscus based on this.展开更多
To explore the effect of different additive combinations on double-low rapeseed cake (meal) utilization and layer production performance, we randomly divided 756 Roman brown shell layers around 50 weeks age into sev...To explore the effect of different additive combinations on double-low rapeseed cake (meal) utilization and layer production performance, we randomly divided 756 Roman brown shell layers around 50 weeks age into seven groups to detect production performance, of which group 1 was control group supplied with basic diet (added with 9% double-low rapeseed cake), while groups 2 -7 were experimental groups added with Chinese herbal medicine, enzyme preparation, pro- biotics and peptide antibiotics in basic diet. The results showed that group 4 added with Chinese herbal medicine + enzyme preparation + peptide antibiotics re- ceived the best effect, resulting in significantly increased laying rate (70.50%, P 〈 0.05 ), significantly reduced feed-egg ratio (2.46, P 〈 0.05 ), and extremely increased egg Haugh unit (P 〈0.01 ). This indicated that adding combination of Chinese herbal medicine + enzyme preparation + peptide antibiotics in layer bas- ic diet could improve egg production performance and egg quality of layers.展开更多
Aladan {β-[6'-(N,N-dimethyl)amino-2'-naphthoyl]alanine} and aladan-containing cyclodecapeptide [Ald^6]loloatin C were synthesized. The properties of their fluorescent behaviours both in aqueous buffer and micelle...Aladan {β-[6'-(N,N-dimethyl)amino-2'-naphthoyl]alanine} and aladan-containing cyclodecapeptide [Ald^6]loloatin C were synthesized. The properties of their fluorescent behaviours both in aqueous buffer and micelles created by sodium dodecylsulfate (SDS) and dodecylphosphorylcholine (DPC) were further investigated. It was found that in either of micelle systems, [Ald^6]loloatin C possesses red-shift emission maximum wavelength and higher quantum yield. This property could be useful in the research of peptide-cell membrane interaction.展开更多
基金Supported by Agricultural Research Project of Science and Technology Department of Zhejiang Province(2008C22026,2009C32016)Open Topic of Key Open Laboratory of Marine and Estuarine Fishery Resources and Ecology, Ministry of Agriculture ( Open-09-10 )+1 种基金Technology Bureau Project of Zhoushan City (Y20082080)Innovative Business Incubation Program of College Students~~
文摘[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides (mytilin and myticin) from Mytilus coruscus.[Method] By the screening of antibacterial peptide genes of mytilin and myticin of Mytilus coruscus,five antibacterial peptide genes were selected.Then,the relative eukaryotic expressing vectors were constructed by the use of PCR technique and DNA recombinant technology.Subsequently,they were transferred in to S78 Saccharomyces cerevisia by using LiAC transformation method,and then preliminary expressing analysis was carried out.[Result] Five eukaryotic expressing vectors of antibacterial peptides from Mytilus coruscus were successfully constructed,and the results of mRNA detection revealed that the five antibacterial peptides from Mytilus coruscus were successfully transcribed.[Conclusion] The results provide a basis for using genetic engineering to express antibacterial peptides of mytilin and myticin from Mytilus coruscus,and for developing the further study of antibacterial peptides from Mytilus coruscus based on this.
基金Supported by Core Pure Population Breeding and Industry Demonstration Base Construction of Changshun Green-shell Layer(QNYZ[2011]027)Association Study of PRL Gene and PRLR Gene Polymorphisms of Changshun Greenshell Layer with Egg Production Traits(QKHJ[2011]2174)
文摘To explore the effect of different additive combinations on double-low rapeseed cake (meal) utilization and layer production performance, we randomly divided 756 Roman brown shell layers around 50 weeks age into seven groups to detect production performance, of which group 1 was control group supplied with basic diet (added with 9% double-low rapeseed cake), while groups 2 -7 were experimental groups added with Chinese herbal medicine, enzyme preparation, pro- biotics and peptide antibiotics in basic diet. The results showed that group 4 added with Chinese herbal medicine + enzyme preparation + peptide antibiotics re- ceived the best effect, resulting in significantly increased laying rate (70.50%, P 〈 0.05 ), significantly reduced feed-egg ratio (2.46, P 〈 0.05 ), and extremely increased egg Haugh unit (P 〈0.01 ). This indicated that adding combination of Chinese herbal medicine + enzyme preparation + peptide antibiotics in layer bas- ic diet could improve egg production performance and egg quality of layers.
文摘Aladan {β-[6'-(N,N-dimethyl)amino-2'-naphthoyl]alanine} and aladan-containing cyclodecapeptide [Ald^6]loloatin C were synthesized. The properties of their fluorescent behaviours both in aqueous buffer and micelles created by sodium dodecylsulfate (SDS) and dodecylphosphorylcholine (DPC) were further investigated. It was found that in either of micelle systems, [Ald^6]loloatin C possesses red-shift emission maximum wavelength and higher quantum yield. This property could be useful in the research of peptide-cell membrane interaction.