OBJECTIVE： Bao-Xie-Ning （BXN）, a traditional Chinese herbal medicine （CHM） formula composed of Fructus Evodiae, Flos Caryophylli and Cortex Cinnamomi, and used for the treatment of infant diarrheal illness, was s...OBJECTIVE： Bao-Xie-Ning （BXN）, a traditional Chinese herbal medicine （CHM） formula composed of Fructus Evodiae, Flos Caryophylli and Cortex Cinnamomi, and used for the treatment of infant diarrheal illness, was subject to systematic assessment for its putative multiple pharmacodynamic effects and pharmacological antidiarrheal mechanisms. METHODS： High-performance liquid chromatography-diode array detector-electrospray ionization- mass spectrometric/mass spectrometry was developed and validated for identification and quantification of the main constituents in different extracts of BXN. Male Kunming mice weighing 20 to 25 g were used for detecting the antidiarrheal activity of the extracts. Ethanolic extract （EE）, volatile oil extract （VOE）, and aqueous extract （AE） of BXN were respectively subjected to pharmacodynamic and pharmacological comparison in assessing antidiarrheal effects with senna-induced diarrhea, castor oil-induced diarrhea, acetic acid-induced writhing assay, and isolated duodenum test. RESULTS： The highest yields of three detected components of BXN, rutaecarpine, eugenol and cinnamaldehyde were observed in EE. EE showed the most remarkable antidiarrheal activity in dose-dependent and time-dependent manners in both senna- and castor oil-induced diarrhea models, and presented dose-dependent analgesic activity in acetic acid-induced algesthesia model. In addition, EE extract of BXN also exhibited strong antimobility action on the intestine and strongest depression on spontaneous contraction of isolated duodenum. CONCLUSION： Ethanol extraction is an efficient method to extract the active constituents of BXN. BXN extract demonstrated multiple pharmacological activities affecting the main mechanisms of diarrhea, which validated BXN＇s usage in the comprehensive clinical treatment of diarrhea.展开更多
Objective:To evaluate the pharmacological activity against diarrhea of methanol and petroleum ether extract ofDesmodium puchellum(Family:Fabaceae)leaves.Methods:The extract was evaluated for castor oil-induced diarrhe...Objective:To evaluate the pharmacological activity against diarrhea of methanol and petroleum ether extract ofDesmodium puchellum(Family:Fabaceae)leaves.Methods:The extract was evaluated for castor oil-induced diarrhea and enteropooling as well as intestinal motility in rats.Both of the extracts were given to the rats at 200 mg/kg orally.Loperamide was used as a standard drug for diarrhea.Results:The diarrheal severity was reduced significantly(P<0.05)by methanol and petroleum ether extracts by 31.95% and 28.33%,respectively,whereas 54.75% inhibition was found for standard drug loperamide at 5 mg/kg.The two extracts also significantly(P<0.05) reduced the intestinal volume in case of castor oil induced enteropooling.Conclusions:It is concluded that both fractions contain some biologically active ingredients that are active for anti-diarrheal actions whereas methanol fraction has better potential.展开更多
基金supported and funded by the State Administration of Traditional Chinese Medicine of China (No.2003LHR20)
文摘OBJECTIVE： Bao-Xie-Ning （BXN）, a traditional Chinese herbal medicine （CHM） formula composed of Fructus Evodiae, Flos Caryophylli and Cortex Cinnamomi, and used for the treatment of infant diarrheal illness, was subject to systematic assessment for its putative multiple pharmacodynamic effects and pharmacological antidiarrheal mechanisms. METHODS： High-performance liquid chromatography-diode array detector-electrospray ionization- mass spectrometric/mass spectrometry was developed and validated for identification and quantification of the main constituents in different extracts of BXN. Male Kunming mice weighing 20 to 25 g were used for detecting the antidiarrheal activity of the extracts. Ethanolic extract （EE）, volatile oil extract （VOE）, and aqueous extract （AE） of BXN were respectively subjected to pharmacodynamic and pharmacological comparison in assessing antidiarrheal effects with senna-induced diarrhea, castor oil-induced diarrhea, acetic acid-induced writhing assay, and isolated duodenum test. RESULTS： The highest yields of three detected components of BXN, rutaecarpine, eugenol and cinnamaldehyde were observed in EE. EE showed the most remarkable antidiarrheal activity in dose-dependent and time-dependent manners in both senna- and castor oil-induced diarrhea models, and presented dose-dependent analgesic activity in acetic acid-induced algesthesia model. In addition, EE extract of BXN also exhibited strong antimobility action on the intestine and strongest depression on spontaneous contraction of isolated duodenum. CONCLUSION： Ethanol extraction is an efficient method to extract the active constituents of BXN. BXN extract demonstrated multiple pharmacological activities affecting the main mechanisms of diarrhea, which validated BXN＇s usage in the comprehensive clinical treatment of diarrhea.
基金Financially supported by the Bangladesh Council of Scientific and Industrial Research(BCSIR),Chittagong(Grant No.1217)
文摘Objective:To evaluate the pharmacological activity against diarrhea of methanol and petroleum ether extract ofDesmodium puchellum(Family:Fabaceae)leaves.Methods:The extract was evaluated for castor oil-induced diarrhea and enteropooling as well as intestinal motility in rats.Both of the extracts were given to the rats at 200 mg/kg orally.Loperamide was used as a standard drug for diarrhea.Results:The diarrheal severity was reduced significantly(P<0.05)by methanol and petroleum ether extracts by 31.95% and 28.33%,respectively,whereas 54.75% inhibition was found for standard drug loperamide at 5 mg/kg.The two extracts also significantly(P<0.05) reduced the intestinal volume in case of castor oil induced enteropooling.Conclusions:It is concluded that both fractions contain some biologically active ingredients that are active for anti-diarrheal actions whereas methanol fraction has better potential.
