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Comparisons of voided urine cytology, nuclear matrix protein-22 and bladder tumor associated antigen tests for bladder cancer of geriatric male patients in Taiwan, China 被引量:7
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作者 Ke-Hung Tsui Shao-Ming Chen +4 位作者 Ta-Ming Wang Horng-Heng Juang Chien-Lun Chen Guang-Huan Sun Phei-Lang Chang 《Asian Journal of Andrology》 SCIE CAS CSCD 2007年第5期711-715,共5页
Aim: To compare the results of bladder tumor associated antigen (BTA TRAK), nuclear matrix protein 22 (NMP 22) and voided urine cytology (VUC) in detecting bladder cancer. Methods: A total of 135 elderly male ... Aim: To compare the results of bladder tumor associated antigen (BTA TRAK), nuclear matrix protein 22 (NMP 22) and voided urine cytology (VUC) in detecting bladder cancer. Methods: A total of 135 elderly male and 50 healthy volunteers enrolled in this study were classified into three groups: (i) 93 patients with bladder cancer; (ii) 42 patients with urinary benign conditions; and (iii) 50 healthy volunteers. BTA TRAK and NMP 22 kits were used to detect bladder cancer. Voided urine cytology was used to compare the sensitivity and specificity of the screening tests. Results: The sensitivity and specificity of cytology, BTA TRAK and NMP 22 were 24% and 97%, 51% and 73%, 78% and 73%, respectively. The level of NMP 22 increased with tumor grading. The BTA TRAK kit has the lowest sensitivity among the screening tests. The NMP 22 with the best sensitivity can be an adjunct to cytology for evaluating bladder cancer. Conclusion: The NMP 22 test has a better correlation with the grading of the bladder cancer than BTA TRAK. As cytology units are typically not available in hospitals or in outpatient clinics, NMP 22 might be a promising tool for screening bladder cancer. 展开更多
关键词 bladder neoplasm CYTOLOGY bladder tumor associated antigen nuclear matrix protein 22
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Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella 被引量:2
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作者 罗璋 刘志新 +3 位作者 付建平 张秋胜 黄贝 聂品 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2016年第6期1247-1257,共11页
Flavobacterium columnare causes columnaris disease in freshwater fi sh. In the present study, the antigenic regions of fi ve outer membrane proteins(OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermo... Flavobacterium columnare causes columnaris disease in freshwater fi sh. In the present study, the antigenic regions of fi ve outer membrane proteins(OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli. The expressed protein of 95.6 k Da, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purifi ed recombinant protein was used to vaccinate the grass carp, C tenopharyngodon idella. Following vaccination of the fi sh their Ig M antibody levels were examined, as was the expression of I g M, Ig D and Ig Z immunoglobulin genes and other genes such as MHC Iα and MHC I I β, which are also involved in adaptive immunity. Interleukin genes( IL), including I L- 1β, IL- 8 and I L- 10, and type I and type II interferon(I FN) genes were also examined. At 3 and 4 weeks post-vaccination(wpv), signifi cant increases in Ig M antibody levels were observed in the fi sh vaccinated with the recombinant fusion protein, and an increase in the expression levels of I g M, Ig D and Ig Z genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fi sh. At four wpv, the fi sh were challenged with F. column a re, and the vaccinated fi sh showed a good level of protection against this pathogen, with 39% relative percent survival(RPS) compared with the control group. It can be concluded, therefore, that the fi ve OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fi sh and protection against F. columnare. 展开更多
关键词 Flavobacterium columnare outer membrane protein antigen immunogenicity vaccine immune response grass carp
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Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer 被引量:1
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作者 Ausra Lukosiute-Urboniene Aldona Jasukaitiene +3 位作者 Giedre Silkuniene Vidmantas Barauskas Antanas Gulbinas Zilvinas Dambrauskas 《World Journal of Gastroenterology》 SCIE CAS 2019年第2期205-219,共15页
AIM To determine the association of human antigen R(HuR) and inhibitors of apoptosis proteins(IAP1, IAP2) and prognosis in pancreatic cancer.METHODS Protein and mRNA expression levels of IAP1, IAP2 and HuR in pancreat... AIM To determine the association of human antigen R(HuR) and inhibitors of apoptosis proteins(IAP1, IAP2) and prognosis in pancreatic cancer.METHODS Protein and mRNA expression levels of IAP1, IAP2 and HuR in pancreatic ductal adenocarcinoma(PDAC) were compared with normal pancreatic tissue. The correlations among IAP1/IAP2 and HuR as well as their respective correlations with clinicopathological parameters were analyzed. The Kaplan-Meier method and log-rank tests were used for survival analysis. Immunoprecipitation assay was performed to demonstrate HuR binding to IAP1, IAP2 mRNA. PANC1 cells were transfected with either anti-HuR siRNA or control siRNA for 72 h and quantitative reverse transcription polymerase chain reaction(RT-PCR), western blot analysis was carried out.RESULTS RT-PCR analysis revealed that HuR, IAP1, IAP2 mRNA expression were accordingly 3.3-fold, 5.5-fold and 8.4 higher in the PDAC when compared to normal pancreas(P < 0.05). Expression of IAP1 was positively strongly correlated with HuR expression(P < 0.05, r = 0.783). Western blot analysis confirmed RTPCR results. High IAP1 expression, tumor resection status, T stage, lymph-node metastases, tumor differentiation grade, perineural and lymphatic invasion were identified as significant factors for shorter survival in PDAC patients(P < 0.05).Immunohistological analysis showed that HuR was mainly expressed in the ductal cancer cell's nucleus and less so in cytoplasm. RNA immunoprecipitation analysis confirmed IAP1 and IAP2 post-transcriptional regulation by HuR protein. Following siHuR transfection, IAP1 mRNA and protein levels were decreased, however IAP2 expression levels were increased.CONCLUSION HuR mediated overexpression of IAP1 significantly correlates with poor outcomes and early progression of pancreatic cancer. Further studies are needed to assess the underlying mechanisms. 展开更多
关键词 PANCREATIC cancer Inhibitors of apoptosis proteinS HUMAN antigen R POSTTRANSCRIPTIONAL regulation
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AN HBV LARGE SURFACE ANTIGEN PROTEIN WHICH CAN BE SECRETED FROM MAMMALIAN CELLS
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作者 俞贤明 汪垣 李载平 《Science China Chemistry》 SCIE EI CAS 1992年第4期455-462,共8页
The N-terminal 54 base pairs (encoding amino acid residues 2—19) within the preS1 region of the human hepatitis B virus surface antigen gene were deleted by site-directed mutagenesis. Unlike the wild type large surfa... The N-terminal 54 base pairs (encoding amino acid residues 2—19) within the preS1 region of the human hepatitis B virus surface antigen gene were deleted by site-directed mutagenesis. Unlike the wild type large surface antigen protein; when this mutated gene was expressed in monkey kidney cell line COS-M6, the protein product (S301 protein) could be secreted from the cells. Moreover, the inhibition of the secretion of the major surface antigen protein by this altered large surface antigen protein was greatly reduced, suggesting that the deleted region contained a retention sequence which prevented the secretion of the large surface antigen. However, the coexpression of the major S protein was essential for the secretion of the S301 protein. When coexpressed, the secretion of these two proteins was synchronous. Like the wild type large surface antigen protein, the S301 protein could be translocated into ertdoplasmic reticulum and glycosylated after its synthesis in COS cells. The S301 protein was thermostable and proteinase-resistant. It also retained the antigenicity of the large S and major S proteins. Given the fact that the S301 protein is readily secretable, stable and identical to the large S protein in terms of their antigenicity, it may be developed into a new generation of recombinant vaccine for the prevention of viral hepatitis. 展开更多
关键词 HEPATITIS B virus LARGE SURFACE antigen protein secretability retention sequence.
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Abnormal expressions of proliferating cell nuclear antigen and P27 protein in brain glioma
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作者 Yong feng Hu Wei Shi +3 位作者 Chong xiao Liu Jian jun Sun Rui zhi Wang Zhen yu Guo 《Neural Regeneration Research》 SCIE CAS CSCD 2007年第10期591-594,共4页
Both proliferating cell nuclear antigen and P27 protein are important factors to regulate cell cycle. While, the combination of them can provide exactly objective markers to evaluate prognosis of patients with brain g... Both proliferating cell nuclear antigen and P27 protein are important factors to regulate cell cycle. While, the combination of them can provide exactly objective markers to evaluate prognosis of patients with brain glioma needs to be further studied based on pathological level. OBJECTIVE: To observe the expressions of proliferating cell nuclear antigen and P27 protein in both injured and normal brain glioma tissues and analyze the effect of them on onset and development of brain glioma. DESIGN: Case contrast observation. SETTING: Department of Neurosurgery, the Second Affiliated Hospital of Xi'an Jiaotong University. PARTICIPANTS: A total of 63 patients with brain glioma were selected from Department of Neurosurgery, the Second Affiliated Hospital of Xi'an Jiaotong University from July 1996 to June 2000. There were 38 males and 25 females and their ages ranged from 23 to 71 years. Based on pathological classification and grading standards of brain glioma, patients were divided into grade I - II (n=30) and grade III- IV (n = 33). All cases received one operation but no radiotherapy and chemiotherapy before operation. Sample tissues were collected from tumor parenchyma. Non-neoplastic brain tissues were collected from another 12 non-tumor subjects who received craniocerebral trauma infra-decompression and regarded as the control group. There were l0 males and 2 females and their ages ranged from 16 to 54 years. The experiment had got confirmed consent from local ethic committee and the collection was provided confirmed consent from patients and their relatives. All samples were restained with HE staining so as to diagnose as the brain glioma. While, all patients with brain glioma received radiotherapy after operation and their survival periods were followed up. METHODS: Primary lesion wax of brain glioma was cut into serial sections and stained with S-P immunohistochemical staining. Brown substance which was observed in tumor nucleus was regarded as the positive expressions of both proliferating cell nuclear antigen and P27 protein. Automatic imaging analytic system was used to quantitatively analyze staining results of tumor. MAIN OUTCOME MEASURES: To compare the expressions of proliferating cell nuclear antigen and P27 protein in brain glioma tissues and non-tumor brain tissues and investigate the effect of various sexes, ages, survival periods and severities on the expressions of them in brain tissues. RESULTS: There was no significant difference of sexes and ages in the expressions of proliferating cell nuclear antigen and P27 protein (P 〉 0.05); however, the expressions of proliferating cell nuclear antigen and P27 protein were milder in non-tumor brain tissues than those in the brain glioma tissues (P 〈 0.05). Expression of proliferating cell nuclear antigen in brain tissue of grade III- IV severity was stronger than that of grade I - II severity, and the expression in ≥ 5-year survival periods were also stronger than that in 〈 5-year survival periods (P 〈 0.05). In addition, expression of P27 protein in brain tissue of grade III- IV severity was stronger than that of grade I - II severity, and the expression in ≥ 5-year survival periods were also stronger than that in 〈 5-year survival periods (P 〈 0.05). CONCLUSION: Abnormal expressions of proliferating cell nuclear antigen and P27 protein in human brain glioma are closely related to onset, development and prognosis of tumor. 展开更多
关键词 brain glioma proliferating cell nuclear antigen P27 protein pathological grade PROGNOSIS
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EXPRESSION OF P53 PROTEIN AND PROLIFERATING CELL NUCLEAR ANTIGEN IN HUMAN GESTATION TROPHOBLASTIC DISEASE 被引量:2
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作者 黄铁军 王志忠 +1 位作者 方光光 刘志恒 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2004年第2期147-149,共3页
To study the relationship between p53 protein, proliferating cell nuclear antigen (PCNA) expression and benign or malignant gestational trophoblastic disease (MGTD). Methods: The histotomic sections of 48 patients wit... To study the relationship between p53 protein, proliferating cell nuclear antigen (PCNA) expression and benign or malignant gestational trophoblastic disease (MGTD). Methods: The histotomic sections of 48 patients with gestational trophoblastic disease and 24 patients of normal chorionic villi were stained using immunohistochemistry. The monoclonal antibodies were used to determine p53 protein and PCNA. Results: The frequency of p53 and PCNA positive expression were significantly different among the chorionic villi of normal pregnancy, hydratidiform mole (HM) and MGTD. But neither p53 nor PCNA has any relation with the clinical staging or metastasis of MGTD. Conclusion: Both P53 and PCNA are valuable in diagnosis of human gestational trophoblastic disease. 展开更多
关键词 p53 protein Proliferating cell nuclear antigen (PCNA) Gestational trophoblastic disease(GTD)
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An efficient fusion protein system for expression of Bacillus anthracis protective antigen as immunogenic and diagnostic antigen
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作者 Vahid Bagheri Hossein Motamedi Masoud Reza Seifiabad Shapouri 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第10期765-768,共4页
Objective:To produce high quantities of recombinant protective antigen(rPA) for human vaccine and diagnosis.Methods:The PA gene was amplified by PCR with pXO1 plasmid as template. The PCR product was cloned into pMAL-... Objective:To produce high quantities of recombinant protective antigen(rPA) for human vaccine and diagnosis.Methods:The PA gene was amplified by PCR with pXO1 plasmid as template. The PCR product was cloned into pMAL-c2X vector using the BamH1 and SalI restriction enzymes.The recombinant plasmid was transformed into Escherichia coli DH5 a strain and then screened for transformation.The expression of protective antigen was analyzed by SDS-PAGE and Western blotting after isopropyl β-D-thiogalactopyranoside(IPTG) induction.Results: The full-length PA gene(2.2 kb) was cloned into pMAL vector system.The recombinant vector was confirmed by restriction enzyme and PCR analysis.The expression of cytoplasmic maltose-binding protein-protective(MBP-P) antigen fusion protein was detected by SDS-PAGE and Western blotting,and obtained a 125 kDa protein band,which was similar to expected size of fusion protein.Conclusions:This expression system can be used in the high production of rPA. After purification and immunization studies,the purified rPA may be used in the development of the human recombinant anthrax vaccine and also in diagnosis of anthrax disease. 展开更多
关键词 BACILLUS ANTHRACIS Fusion protein Protective antigen VACCINE
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Protein expression and antigenicity detection of hepatitis C virus envelope protein E2
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《中国输血杂志》 CAS CSCD 2001年第S1期331-,共1页
关键词 protein expression and antigenicity detection of hepatitis C virus envelope protein E2
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The Secondary Structure of Heated Whey Protein andIts Hydrolysates Antigenicity
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作者 PANG Zhi-hua ZHU Jun +4 位作者 WU Wei-jing WANG Fang REN Fa-zheng ZHANG Lu-da GUO Hui-yuan 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2011年第11期3055-3059,共5页
Fourier transform infrared spectroscopy(FTIR) and circular dichroism(CD) were used to investigate the conformational changes of heated whey protein(WP) and the corresponding changes in the hydrolysates immunoreactivit... Fourier transform infrared spectroscopy(FTIR) and circular dichroism(CD) were used to investigate the conformational changes of heated whey protein(WP) and the corresponding changes in the hydrolysates immunoreactivity were determined by competitive enzyme-linked immunosorbent assay(ELISA).Results showed that the contents of α-helix and β-sheet of WP did not decrease much under mild heating conditions and the antigenicity was relatively high;when the heating intensity increased(70 ℃ for 25 min or 75 ℃ for 20 min),the content of α-helix and β-sheet decreased to the minimum,so was the antigenicity;However,when the WP was heated at even higher temperature and for a longer time,the β-sheet associated with protein aggregation begun to increase and the antigenicity increased correspondingly.It was concluded that the conformations of heated WP and the antigenicity of its hydrolysates are related and the optimum structure for decreasing the hydrolysates antigeniity is the least content of α-helix and β-sheet.Establishing the relationship between the WP secondary structure and WP hydrolysates antigenicity is significant to supply the reference for antigenicity reduction by enzymolysis. 展开更多
关键词 FTIR CD Whey protein Heat Treatment antigenICITY
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Effects of heat shock protein-antigen peptide complexes (HACs) in melanoma B16 cell line on transplanted tumor development in mice
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作者 GONG Shou liang,YANG Ying,LI Xiu juan,FU Shi bo,SUN Zu yue,CHEN Sha li,LI Xiu yi (MH Radiobiology Research Unit,Jilin University,Changchun 130021 China) 《白求恩医科大学学报》 CSCD 北大核心 2001年第5期457-460,共4页
目的 :建立黑色素瘤 B16细胞热休克蛋白 -抗原肽复合物 (HACs)的制备方法并测其抑瘤效应。方法 :应用 Sephacryl S- 2 0 0凝胶过滤制备 HAC粗提物 ,应用 SDS- PAGE纯化 HACs,并测其抑瘤效应。结果 :应用 SDS- PAGE纯化的 HAC6 0、 HAC75... 目的 :建立黑色素瘤 B16细胞热休克蛋白 -抗原肽复合物 (HACs)的制备方法并测其抑瘤效应。方法 :应用 Sephacryl S- 2 0 0凝胶过滤制备 HAC粗提物 ,应用 SDS- PAGE纯化 HACs,并测其抑瘤效应。结果 :应用 SDS- PAGE纯化的 HAC6 0、 HAC75和 HAC97不同程度地降低肿瘤发生率 ,延迟肿瘤发生时间和减慢肿瘤生长速度。结论 :6 0 0 0 0~ 970 0 0 展开更多
关键词 黑色素瘤 B16细胞 热休克蛋白-抗原肽复合物 HACs 抑瘤效应
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The development of a recombinant hybrid protein of Plasmodium falciparum and analysis of its antigenicity and protectivity
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作者 李全贞 李英杰 +3 位作者 谢毅 任大明 毕惠祥 徐秉锟 《Journal of Medical Colleges of PLA(China)》 CAS 1994年第3期161-169,共9页
A hybrid gene encoding several putative protective epitopes from erythrocytic stage antigens (MSA1,MSA2 and RESA) of P. falciparum as well as exgenous T cell enhancer epitopes from interleukin-1 and tetanus toxin was ... A hybrid gene encoding several putative protective epitopes from erythrocytic stage antigens (MSA1,MSA2 and RESA) of P. falciparum as well as exgenous T cell enhancer epitopes from interleukin-1 and tetanus toxin was synthesized chemically. This gene (named HGFC) was cloned and connected with another hybrid gene (HPFA) synthesized previously to make a bigger hybrid gene (HGFCAC). HGFC and HGFCAC were cloned in an expression vector pWR450-1 and transformed into E. Coli JM109. The engineered bacteria could express fusion proteins with molecular weights of 65 and 77 kDa after inducing with isopropylthio-β-D-galactoside (IPTG). The expression rate was about 35% of total bacterial proteins. The expressed products showed sepcific immunological reaction with rabbit antibodies against P. falciparum peptide Glu-Glu-Asn-Val-Glu-His-Asp-Ala (EENVEHDA)by Western blotting. The fusion proteins were pruified by precipitation with amonium sulfate. gel filtration and ion-exchange chromatography and the purity was 82%. The purified protein reacted specifically with mouse immune serum against falciparum blood stage antigens by dot enzyme-linked immunosorbent assay (dot-ELISA).The fusion protein was emulsified with Freund's complete adjuvant (FCA) and used to immunize rabbits. The immune serum can recognize P. falciparum erythrocytic stage antigens of Fcc-1/HN strain and Yunnan strain and had weak cross reaction with P. vivax,but had no reaction with P. cynomlogi and P. berghei antigens. The protective effect of the antibody was tested by in vitro inhibition test to cultured falciparum parasites. Preliminary results indicated that the immune sera could effectively reduce the invasion rates of the parasites to red blood cells and inhibit the growth of the in vitro cultured falciparum parasites. The inhibitory capacity of the immune sera to parasite invasion is enhanced as the amount of the sera increases and the incubation time of the sera with the parasites is prolonged.It was shown that after 72 h incubation at 20% concentration with the parasites, the serum can suppress the multiplication of P.falciparum growth in vitro to a level of 80%.The immune sera caused dispersion of the parasite cytoplasm,atrophy of parasites,agglutination of free merozoites and degeneration of schizonts. 展开更多
关键词 PLASMODIUM FALCIPARUM RECOMBINANT protein HYBRID antigen inhibition test in vitro malarial vaccine
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THE LA ANTIGEN INHIBITS THE ACTIVATION OF THE INTERFERON-INDUCIBLE PROTEIN KINASEPKR BY SEQUESTERING AND UNWINDING DOUBLE-STRANDED RNA
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作者 校秋蓉 《Journal of Pharmaceutical Analysis》 CAS 1995年第2期192-193,共2页
The La (SS-B) autoimmune antigen is an RNA-binding protein that is present in both nucleus and cytoplasm of eukaryotic cells.The spectrum of RNAs that interact with the antigen includes species which also bind to the ... The La (SS-B) autoimmune antigen is an RNA-binding protein that is present in both nucleus and cytoplasm of eukaryotic cells.The spectrum of RNAs that interact with the antigen includes species which also bind to the interferon-inducible protein kinase PKR.We have investigated whether the La antigen can regulate the activity of PKR and have observed that both the autophosphorylation of the protein kinase that accompanies its activation by dsRNA and the dsRNA-dependent phosphorylation of the subunit of polypeptide chain initiation factor eIF-2 by PKR are inhibited in the presence of recombinant La antigen. This inhibition is partially relieved at higher concentrations of dsRNA.Once activated by dsRNA the protein kinase activity of PKR is insensitive to the La antigen. We have demonstrated by a filter binding assay that La is a dsRNA binding protein.Furthermore, when recombinant La is incubated with a 900bp synthetic dsRNA or with naturally occurring reovirus dsRNA it converts these substrates to single-stranded forms.We conclude that the La antigen inhibits the dsRNA-dependent activation of PKR by binding and unwinding dsRNA and that it may therefore play a role in the regulation of this protein kinase in interferon-treated or virus-infected cells. 展开更多
关键词 PKR DSRNA protein kinase PHOSPHORYLATION La antigen
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<i>Mycoplasma hominis</i>Variable Adherence-Associated Antigen: A Major Adhesin and Highly Variable Surface Membrane Protein
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作者 Rebecca J. Brown Victoria J. Chalker Owen B. Spiller 《Advances in Microbiology》 2014年第11期736-746,共11页
Mycoplasma hominis is a member of the genus mycoplasma and has only been isolated from humans. It is most frequently isolated from the urogenital tract in the absence of symptoms, but has been isolated from wounds, br... Mycoplasma hominis is a member of the genus mycoplasma and has only been isolated from humans. It is most frequently isolated from the urogenital tract in the absence of symptoms, but has been isolated from wounds, brain abscess, inflamed joints, blood and placenta from pregnancy with adverse outcomes (especially preterm birth and occasionally term stillbirth). Controversy surrounds whether this organism is a commensal or a pathogen;however, Mycoplasma hominis has been shown to induce preterm birth and foetal lung injury in an experimental primate model as a sole pathogen. These bacteria are known to exist as a parasitic infection, due to a number of missing synthetic and metabolism pathway enzymes from their minimal genome;therefore, the ability to adhere to host cells is important. Here we provide a review that clarifies the different nomenclature (variable adherence-associated antigen and P50) that has been used to investigate the major surface adhesin for this organism, as well as reported mechanisms responsible for turning off its expression. Variation in the structure of this protein can be used to separate strains into six categories, a method that we were able to use to distinguish and characterise 12 UK strains isolated from between 1983 and 2012. We propose that the Vaa should be used in further investigations to determine if commensal populations and those that are associated with disease utilise different forms of this adhesin, as this is under-studied and identification of pathogenic determinants is overdue for this organism. 展开更多
关键词 Mycoplasma hominis Variable-Adherence Associated antigen HOST-PATHOGEN Interaction SURFACE Expressed proteins
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猪细小病毒病研究进展
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作者 刘运超 陈玉梅 +3 位作者 杨苏珍 魏蔷 郝慧芳 柴书军 《动物医学进展》 北大核心 2024年第3期107-110,共4页
猪细小病毒(Porcine parvovirus,PPV)是一种无囊膜DNA病毒,主要引起母猪繁殖障碍。该病毒在全世界广泛流行,我国猪场PPV感染率高达90%以上,给养猪业带来巨大经济损失。PPV经口、鼻传播,主要侵染猪的生殖器官,引起母猪的死胎、木乃伊胎... 猪细小病毒(Porcine parvovirus,PPV)是一种无囊膜DNA病毒,主要引起母猪繁殖障碍。该病毒在全世界广泛流行,我国猪场PPV感染率高达90%以上,给养猪业带来巨大经济损失。PPV经口、鼻传播,主要侵染猪的生殖器官,引起母猪的死胎、木乃伊胎和公猪的精液质量下降。临床采用接种疫苗的方式进行防控,起到了一定的效果。论文对病毒的基因组和蛋白特征、流行病学和疫苗研究进行综述,以期为PPV的基础研究和疫苗开发提供参考。 展开更多
关键词 猪细小病毒 VLP组装 病毒抗原表位 VP2蛋白
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膀胱肿瘤抗原、尿核基质蛋白22、细胞角蛋白-19对膀胱癌的诊断价值分析
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作者 王晓甫 张云翔 +3 位作者 张胜威 赵永立 时新宇 许长宝 《癌症进展》 2024年第15期1667-1670,共4页
目的分析膀胱肿瘤抗原(BTA)、尿核基质蛋白22(NMP22)、细胞角蛋白-19(CK-19)对膀胱癌的诊断价值。方法选取53例膀胱癌患者作为膀胱癌组,49例泌尿系良性疾病患者作为对照组。比较两组患者CK-19、NMP22及BTA水平,分析三者水平与膀胱癌患... 目的分析膀胱肿瘤抗原(BTA)、尿核基质蛋白22(NMP22)、细胞角蛋白-19(CK-19)对膀胱癌的诊断价值。方法选取53例膀胱癌患者作为膀胱癌组,49例泌尿系良性疾病患者作为对照组。比较两组患者CK-19、NMP22及BTA水平,分析三者水平与膀胱癌患者临床特征的关系,分析CK-19、NMP22及BTA单独及联合检测对膀胱癌的诊断价值。结果膀胱癌组患者尿液NMP22、BTA及血清CK-19水平均明显高于对照组,差异均有统计学意义(P﹤0.01)。肿瘤分期为Ⅲ~Ⅳ期的膀胱癌患者尿液NMP22、BTA及血清CK-19水平均明显高于Ⅰ~Ⅱ期患者,差异均有统计学意义(P﹤0.01)。CK-19、NMP22及BTA联合检测诊断膀胱癌的灵敏度和特异度分别为0.805和0.869,曲线下面积(AUC)为0.854(95%CI:0.774~0.934),均高于CK-19、NMP22及BTA单独检测。结论膀胱癌患者CK-19、NMP22及BTA水平显著升高,三者水平升高与肿瘤分期相关,密切监测三者水平变化可为临床诊断膀胱癌提供依据。 展开更多
关键词 膀胱癌 膀胱肿瘤抗原 尿核基质蛋白22 细胞角蛋白-19 诊断价值
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Combined Detection of Serum Heat Shock Protein-90<i>α</i>and Prostate Specific Antigen for Prostate Cancer Diagnosis
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作者 Shichang Zhang Jiang Zheng 《Yangtze Medicine》 2020年第4期284-292,共9页
<strong>Objective:</strong> To explore the relationship between heat shock protein-90<i>α</i> (HSP-90<i>α</i>) and occurrence of prostate cancer, and clinical value of combined de... <strong>Objective:</strong> To explore the relationship between heat shock protein-90<i>α</i> (HSP-90<i>α</i>) and occurrence of prostate cancer, and clinical value of combined detection of serum HSP-90<i>α</i> and prostate specific antigen (PSA) in the diagnosis of prostate cancer. <strong>Method:</strong> A total of 30 patients with prostate cancer, 30 patients with benign prostatic hyperplasia (BPH) and 30 healthy men (control group) were selected from September 2018 to September 2019, then to detect levels of serum HSP-90<i>α</i>, total PSA and free PSA (FPSA) by ELISA, serum testosterone level by radioimmunoassay, prostate cancer tissue was removed by operation, and relative expression of tissue HSP-90<i>α</i> protein by Western blot. <strong>Results:</strong> The levels of serum HSP-90<i>α</i> and total PSA in prostate cancer group were significantly higher than other two groups, and testosterone level was lower than other two groups (P < 0.05);there was no difference of serum FPSA level between the three groups (P > 0.05). It was found by Pearson test that serum HSP-90<i>α</i> was positively correlated with total PSA level (r = 0.659, P = 0.005), while negatively correlated with testosterone level (r = -0.549, P = 0.006). According to TNM stage of prostate cancer, there were 17 cases of stage I - II, 13 cases of stage III - IV, 6 cases of Gleason score 1 - 4, 13 cases of 5 - 7, 11 cases of 8 - 10, tumor diameter range from 0.8 to 6.2 cm, with average of (3.9 ± 1.5) cm. The relative expression of HSP-90<i>α</i> protein in tumor tissue was closely related to TNM stage, Gleason score and tumor diameter (P < 0.05). By ROC analysis, it was found that accuracy of combined detection of serum HSP-90<i>α</i> and PSA levels for prostate cancer diagnosis was 0.896, and that of single PSA detection was 0.852. <strong>Conclusion:</strong> Higher expressions of HSP-90<i>α</i> in prostate cancer tissue and serum may be closely related to occurrence and development of prostate cancer, and combined detections of serum HSP-90<i>α</i> and PSA levels are of great significance in improving early diagnosis of prostate cancer. 展开更多
关键词 Heat Shock protein-90α Prostate Specific antigen Prostate Cancer
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临床特征联合血清CA19-9、HE4对子宫内膜异位症相关卵巢癌症的诊断价值
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作者 陈丽 赵威 +2 位作者 李珊珊 梁爽 丁瑞敏 《海南医学》 CAS 2024年第1期76-79,共4页
目的 探讨临床特征联合血清糖类抗原(CA) 19-9、人附睾蛋白4 (HE4)对子宫内膜异位症相关卵巢癌症(EAOC)的诊断价值。方法 选择2020年1月至2022年12月在河南中医药大学第三附属医院接受手术且经术后病理确诊为EAOC的43例患者作为观察组,... 目的 探讨临床特征联合血清糖类抗原(CA) 19-9、人附睾蛋白4 (HE4)对子宫内膜异位症相关卵巢癌症(EAOC)的诊断价值。方法 选择2020年1月至2022年12月在河南中医药大学第三附属医院接受手术且经术后病理确诊为EAOC的43例患者作为观察组,按照1∶2的比例抽取同期在我院手术且经术后证实为卵巢子宫内膜异位症(OEM)的86例患者作为对照组。比较两组患者的临床资料及血清CA125、CA19-9和HE4水平,采用多因素Logistic回归分析影响EAOC的独立风险因素,并采用受试者工作特征曲线(ROC)分析临床特征、CA19-9和HE4诊断EAOC的价值。结果 观察组患者的CA19-9和HE4水平分别为[20.99 (17.06,32.40)] U/mL和[64.47 (55.93,72.01)] U/mL,明显高于对照组的[4.98(2.18,10.86)] U/mL和[43.39(34.61,52.40)] U/mL,差异均有统计学意义(P<0.05);观察组患者的CA125水平为[59.85 (39.51,92.26)] pmol/L,明略高于对照组的[56.58 (39.80,80.68)] pmol/L,但差异无统计学意义(P>0.05)。经多因素Logistic回归分析结果显示,CA19-9、HE4、年龄、肿瘤最长径是影响EAOC的风险因素(P<0.05)。经ROC分析结果显示,年龄、肿瘤最长径、CA19-9、HE4联合检测的曲线下面积(AUC)为0.958,明显高于单独检测(年龄:0.857;肿瘤最长径:0.767;CA19-9:0.767;HE4:0.808)(P<0.05)。结论 CA19-9、HE4、年龄、肿瘤最长径可用于预测EAOC,联合检测可提高诊断效能。 展开更多
关键词 子宫内膜异位症 子宫内膜异位症相关卵巢癌症 糖类抗原19-9 人附睾蛋白4 诊断价值
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血清HE4、CA125、TK1水平在卵巢癌患者中的相关性分析
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作者 蔡清华 王兴祖 董翠莲 《齐齐哈尔医学院学报》 2024年第15期1441-1444,共4页
目的探讨人附睾蛋白4(HE4)、糖类抗原125(CA125)和胸苷激酶1(TK1)联合检测在卵巢癌患者中的相关性。方法选择2022年1月—2023年11月本院收治的通过影像及病理活检被明确诊断为卵巢癌的53例患者(卵巢癌组)和良性肿瘤的29例患者(良性对照... 目的探讨人附睾蛋白4(HE4)、糖类抗原125(CA125)和胸苷激酶1(TK1)联合检测在卵巢癌患者中的相关性。方法选择2022年1月—2023年11月本院收治的通过影像及病理活检被明确诊断为卵巢癌的53例患者(卵巢癌组)和良性肿瘤的29例患者(良性对照组)作为研究对象;另选同期健康管理中心接受体检的100名健康妇女作为健康对照组。采用化学发光法检测血清中HE4、CA125和免疫印迹法检测TK1水平,分别比较三组及卵巢癌不同分期血清HE4、CA125、TK1的水平以及ROC曲线对血清HE4、CA125、TK1及联合检测对卵巢癌诊断效能的价值分析。结果卵巢癌患者血清中HE4、CA125、TK1水平均明显高于良性对照组和健康对照组(P<0.05);卵巢癌组中Ⅲ~Ⅳ期患者血清中HE4、CA125、TK1水平均明显高于Ⅰ~Ⅱ期患者(P<0.05),TK1水平在卵巢癌分组中差异无统计学意义(P>0.05);经ROC曲线分析发现,血清中HE4、CA125、TK1联合检测对卵巢癌诊断效能优于三项单独检测。结论卵巢癌患者血清中HE4、CA125、TK1的水平异常表达,且HE4、CA125、TK1三者联合检测的诊断价值效能明显优于单独检测,有助于提高临床对卵巢癌早期筛查的准确率以及协助卵巢癌患者预后的评估,以期提高患者的生存率。 展开更多
关键词 卵巢癌 HE4 CA125 TK1 联合检测
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血清S100A11、S100A14、CA125与ⅡB~Ⅳ期上皮性卵巢癌患者减瘤术结局的关系及其预测价值分析
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作者 陆泓 尹翼 +1 位作者 李铃铃 李咏 《国际检验医学杂志》 CAS 2024年第19期2372-2376,2381,共6页
目的 探讨ⅡB~Ⅳ期上皮性卵巢癌(EOC)患者血清S100钙结合蛋白A(S100A)11、S100A14和糖类抗原125(CA125)水平与肿瘤细胞减灭术(简称减瘤术)结局的关系及其预测价值。方法 选取2018年6月至2021年6月于该院接受初始减瘤术的ⅡB~Ⅳ期的124例... 目的 探讨ⅡB~Ⅳ期上皮性卵巢癌(EOC)患者血清S100钙结合蛋白A(S100A)11、S100A14和糖类抗原125(CA125)水平与肿瘤细胞减灭术(简称减瘤术)结局的关系及其预测价值。方法 选取2018年6月至2021年6月于该院接受初始减瘤术的ⅡB~Ⅳ期的124例EOC患者作为EOC组及70例卵巢良性病变患者作为病例对照组,并选取同期于该院体检的体检健康者70例作为健康对照组。检测各组血清S100A11、S100A14、CA125水平。根据EOC患者减瘤术结局是否满意,分为满意组(70例)和不满意组(54例)。分析ⅡB~Ⅳ期EOC患者减瘤术结局不满意的影响因素,并评估血清S100A11、S100A14、CA125预测ⅡB~Ⅳ期EOC患者减瘤术结局不满意的临床价值。结果 EOC组血清S100A11、S100A14、CA125水平高于病例对照组及健康对照组,差异有统计学意义(P<0.05)。不满意组美国麻醉医师协会(ASA)分级Ⅲ级、腹水、国际妇产科联盟(FIGO)分期ⅢC~Ⅳ期比例及血清CA125、S100A11、S100A14水平均高于满意组,差异有统计学意义(P<0.05)。FIGO分期ⅢC~Ⅳ期及血清CA125、S100A11、S100A14水平升高是影响EOC患者减瘤术结局不满意的独立危险因素(P<0.05)。血清CA125、S100A11、S100A14及三者联合预测EOC患者减瘤术结局不满意的曲线下面积(95%CI)分别为0.727(0.521~0.910)、0.747(0.507~0.961)、0.755(0.553~0.954)、0.825(0.743~0.913),三者联合检测预测减瘤术结局不满意的价值优于血清CA125、S100A11、S100A14单独检测。结论 ⅡB~Ⅳ期EOC患者血清S100A11、S100A14、CA125水平升高,与减瘤术结局不满意有关,血清CA125、S100A11、S100A14联合检测对减瘤术结局不满意具有较高的预测价值。 展开更多
关键词 上皮性卵巢癌 肿瘤细胞减灭术 S100钙结合蛋白A11 S100钙结合蛋白A14 糖类抗原125
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结直肠癌组织中HMGB2、MAGE-A9、MMR蛋白表达及其临床意义
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作者 祁晓星 徐丹丹 卞建强 《实用癌症杂志》 2024年第3期421-424,共4页
目的探讨结直肠癌组织中高迁移率族蛋白B2(HMGB2)、黑色素瘤相关抗原-A9(MAGE-A9)、DNA错配修复基因(MMR)蛋白表达情况,以及其在评估患者预后中的临床价值。方法选取52例结直肠癌患者作为研究对象,术中采集所有患者肿瘤标本及癌旁组织标... 目的探讨结直肠癌组织中高迁移率族蛋白B2(HMGB2)、黑色素瘤相关抗原-A9(MAGE-A9)、DNA错配修复基因(MMR)蛋白表达情况,以及其在评估患者预后中的临床价值。方法选取52例结直肠癌患者作为研究对象,术中采集所有患者肿瘤标本及癌旁组织标本,检测HMGB2、MAGE-A9、MMR蛋白表达水平,并分析各指标表达情况与病理特征、预后间的关系。结果肿瘤组织内HMGB2、MAGE-A9、MMR阳性表达高于癌旁组织,差异有统计学意义(P<0.05)。肿瘤分期Ⅲ期、分化程度低分化、淋巴结转移患者HMGB2、MAGE-A9、MMR阳性表达率高于Ⅰ期、Ⅱ期、中高分化、无淋巴结转移患者,差异有统计学意义(P<0.05)。随访3年,52例患者存活率为71.15%(37/52);HMGB2阳性组存活率[61.76%(21/34)]低于HMGB2阴性组[88.89%(16/18)],差异有统计学意义(χ^(2)=4.219,P=0.040);MAGE-A9阳性组存活率[58.62%(17/29)]低于MAGE-A9阴性组[86.96%(20/23)],差异有统计学意义(χ^(2)=5.018,P=0.025);MMR阳性组存活率[52.00%(13/25)]低于MMR阴性组[88.89%(24/27)],差异有统计学意义(χ^(2)=8.606,P=0.003)。结论HMGB2、MAGE-A9、MMR在结直肠癌中呈高表达状态,其表达与肿瘤分期、分化程度、淋巴结转移关系密切,且不同表达患者存活率存在较大差异,可作为评估预后的重要指标。 展开更多
关键词 结直肠癌 黑色素瘤相关抗原-A9 高迁移率族蛋白B2 DHA错配修复基因 临床病理特征 预后
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