Objective:To investigate the efficacy of ethanolic extract of nilavembu kudineer choornam (EENKC) in inflammation,pain and fever using animal models to support its actions.Methods: Acute toxicity study of EENKC was pe...Objective:To investigate the efficacy of ethanolic extract of nilavembu kudineer choornam (EENKC) in inflammation,pain and fever using animal models to support its actions.Methods: Acute toxicity study of EENKC was performed in mice to fix the effective dose.The antipyretic, anti-inflammatory and analgesic activity of EENKC was evaluated in brewer’s yeast induced pyrexia in rats,carrageenan-induced inflammation in rats and acetic-acid induced writhing in mice model.Results:Acute toxicity revealed that EENKC didn’t show death and toxic signs up to 2 000 mg/kg.In brewer’s yeast induced pyrexia and carrageenan-induced inflammation EENKC at the doses of 200 and 400 mg/kg inhibited fever and inflammation significantly(P【0.01 and【0.05) compared to control animals.In mice,the number of writhing induced by acetic-acid was significantly(P【0.01) reduced after treatment with both the dose of EENKC than control animals. EENKC 200 mg/kg inhibits inflammation higher level in carrageenan-induced paw edema,but there is no significant difference when compared to indomethacin 10 mg/kg.Conclusions:The present findings revealed that EENKC possesses antipyretic,anti-inflammatory and analgesic activity which supports nilavembu kudineer choornam efficacy in chikungunya fever.展开更多
Objective: To evaluate the other pharmacological actions of silymarin in Albino rats and mice such as antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects. Methods: Rats were injected intramu...Objective: To evaluate the other pharmacological actions of silymarin in Albino rats and mice such as antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects. Methods: Rats were injected intramuscularly with pyrogenic dose of brewer's yeast for the antipyretic test of silymarin. Another group of rats injected with 0.1 mL of 1% carrageenan solution in saline at the subplanter area of the right hind paw for the anti-inflammatory test of silymarin. Another group of mice tested by hot plate method for determination of antinociceptive effect of silymarin. Hyperlipidemia was induced using high fat diet for 2 months to estimate the antihyperlipidemic activity of silymarin. Results: Silymarin showed a significant antipyretic effect of both doses(50 and 100 mg/kg) compared with control untreated group. Moreover, silymarin elucidated a significant anti-inflammatory effect of both doses reflected on the decrease of the rat paw edema every hour interval for 4 h after administration in comparison with control positive group. By the same taken, both doses of silymarine revealed a significant antinociceptive action in hot plate method at 30 and 60 min post administration. Besides, it lowered significantly the serum levels of prostaglandin E2, tumor necrosis factor alpha and interleukin 1 beta after 2 h of silymarin administration in carrageenan induced rat paw edema besides the significant decrease of total cholesterol, triglycerides, low density lipoprotein and significantly elevated high density lipoprotein after 2 weeks of silymarin administration. Conclusions: These outcomes delivered a new vision into the possible pharmacological mechanisms by which silymarin advances antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects.展开更多
OBJECTIVE： The main objectives of this study were to qualitatively evaluate the profile of phytochemical constituents present in methanolic extract of Microcos paniculata bark（BME） and fruit（FME）, as well as to e...OBJECTIVE： The main objectives of this study were to qualitatively evaluate the profile of phytochemical constituents present in methanolic extract of Microcos paniculata bark（BME） and fruit（FME）, as well as to evaluate their anti-inflammatory, analgesic and antipyretic activities.METHODS： Phytochemical constituents of BME and FME were determined by different qualitative tests such as Molisch’s test, Fehling’s test, alkaloid test, frothing test, Fe Cl3 test, alkali test, Salkowski’s test and Baljet test. The anti-inflammatory, analgesic and antipyretic activities of the extracts were evaluated through proteinase-inhibitory assay, xylene-induced ear edema test, cotton pellet-induced granuloma formation in mice, formalin test, acetic acid-induced writhing test, tail immersion test and Brewer’s yeastinduced pyrexia in mice.RESULTS： M. paniculata extracts revealed the presence of carbohydrates, alkaloids, saponins, tannins, flavonoids and triterpenoids. All of the extracts showed significant（P〈0.05, vs aspirin group） proteinaseinhibitory activity, whereas the highest effect elicited by plant extracts was exhibited by the BME（75.94% proteinase inhibition activity） with a half-maximal inhibitory concentration（IC50） of 61.31 μg/m L. Each extract at the doses of 200 and 400 mg/kg body weight showed significant（P〈0.05, vs control） percentage inhibition of ear edema and granuloma formation. These extracts significantly（P〈0.05, vs control） reduced the paw licking and abdominal writhing of mice. In addition, BME 400 mg/kg, and FME at 200 and 400 mg/kg showed significant（P〈0.05, vs control） analgesic activities at 60 min in the tail immersion test. Again, the significant（P〈0.05, vs control） post-treatment antipyretic activities were found by BME 200 and 400 mg/kg and FME 400 mg/kg respectively.CONCLUSION： Study results indicate that M. paniculata may provide a source of plant compounds with anti-inflammatory, analgesic and antipyretic activities.展开更多
OBJECTIVE To isolate and characterize bioactive compound from traditionally important medicinal plant Mikania cordata,and to investigate muti-faced pharmacological activities of the isolated compound.METHODS A triterp...OBJECTIVE To isolate and characterize bioactive compound from traditionally important medicinal plant Mikania cordata,and to investigate muti-faced pharmacological activities of the isolated compound.METHODS A triterpeenoid,16-hydroxy betulinic acid(16 HBA) was isolated from Mikania cordata leaf and the structure of the compound was determined by NMR spectroscopic means.Antimicrobial activity of 16 HBA was tested by disc diffusion method and minimum inhibitory concentration(MIC) against the tested microorganisms was determined.The analgesic property of 16 HBA was tested using acetic acid-induced writhes in mice and hot plate thermal stimulation in rats.The anti-inflammatory activity was studied using carrageenin-induced paw edema method.The antipyretic potential of 16 HBA was evaluated by using yeast-induced hyperthermia in rats.RESULTS The triterpenoid 16 HBA showed potent antibacterial activity with inhibition zone of diameter ranging from 12.0~17.5 mm and antifungal activity with mycelial growth inhibition ranges from 37.6%~54.5%.The MIC values for antibacterial and antifungal activities ranged from 31.5~125 and 250~1000 μg·mL-1 respectively.The compound(50 and100 mg·kg^(-1) body weight) showed potent peripheral and central analgesic activity having 55.19% and41% of writhing inhibition at 90 min after administration of the compound and the highest 55.98%,79.18% elongation of reaction time,respectively.In anti-inflammatory activity screening,the compound(100 mg·kg^(-1)) revealed the highest 77.08% edema inhibition at 4 h after administration of carrageenan.In antipyretic assay,16 HBA exhibited a strong antipyretic effect in yeast-induced rats.CONCLUSION The present study confirmed that 16-hydroxy betulinic acid isolated from Mikania cordata has potent antimicrobial,analgesic,anti-inflammatory and antipyretic properties.Our results can be seen as scientific support for the traditional and folklore usage of Mikania cordata in Bangladesh for the treatment of different ailments and provide opportunities to explore this plant as a source bioactive compounds for biochemical and pharmaceutical industries.展开更多
文摘Objective:To investigate the efficacy of ethanolic extract of nilavembu kudineer choornam (EENKC) in inflammation,pain and fever using animal models to support its actions.Methods: Acute toxicity study of EENKC was performed in mice to fix the effective dose.The antipyretic, anti-inflammatory and analgesic activity of EENKC was evaluated in brewer’s yeast induced pyrexia in rats,carrageenan-induced inflammation in rats and acetic-acid induced writhing in mice model.Results:Acute toxicity revealed that EENKC didn’t show death and toxic signs up to 2 000 mg/kg.In brewer’s yeast induced pyrexia and carrageenan-induced inflammation EENKC at the doses of 200 and 400 mg/kg inhibited fever and inflammation significantly(P【0.01 and【0.05) compared to control animals.In mice,the number of writhing induced by acetic-acid was significantly(P【0.01) reduced after treatment with both the dose of EENKC than control animals. EENKC 200 mg/kg inhibits inflammation higher level in carrageenan-induced paw edema,but there is no significant difference when compared to indomethacin 10 mg/kg.Conclusions:The present findings revealed that EENKC possesses antipyretic,anti-inflammatory and analgesic activity which supports nilavembu kudineer choornam efficacy in chikungunya fever.
文摘Objective: To evaluate the other pharmacological actions of silymarin in Albino rats and mice such as antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects. Methods: Rats were injected intramuscularly with pyrogenic dose of brewer's yeast for the antipyretic test of silymarin. Another group of rats injected with 0.1 mL of 1% carrageenan solution in saline at the subplanter area of the right hind paw for the anti-inflammatory test of silymarin. Another group of mice tested by hot plate method for determination of antinociceptive effect of silymarin. Hyperlipidemia was induced using high fat diet for 2 months to estimate the antihyperlipidemic activity of silymarin. Results: Silymarin showed a significant antipyretic effect of both doses(50 and 100 mg/kg) compared with control untreated group. Moreover, silymarin elucidated a significant anti-inflammatory effect of both doses reflected on the decrease of the rat paw edema every hour interval for 4 h after administration in comparison with control positive group. By the same taken, both doses of silymarine revealed a significant antinociceptive action in hot plate method at 30 and 60 min post administration. Besides, it lowered significantly the serum levels of prostaglandin E2, tumor necrosis factor alpha and interleukin 1 beta after 2 h of silymarin administration in carrageenan induced rat paw edema besides the significant decrease of total cholesterol, triglycerides, low density lipoprotein and significantly elevated high density lipoprotein after 2 weeks of silymarin administration. Conclusions: These outcomes delivered a new vision into the possible pharmacological mechanisms by which silymarin advances antipyretic, anti-inflammatory, antinociceptive and antihyperlipidemic effects.
文摘OBJECTIVE： The main objectives of this study were to qualitatively evaluate the profile of phytochemical constituents present in methanolic extract of Microcos paniculata bark（BME） and fruit（FME）, as well as to evaluate their anti-inflammatory, analgesic and antipyretic activities.METHODS： Phytochemical constituents of BME and FME were determined by different qualitative tests such as Molisch’s test, Fehling’s test, alkaloid test, frothing test, Fe Cl3 test, alkali test, Salkowski’s test and Baljet test. The anti-inflammatory, analgesic and antipyretic activities of the extracts were evaluated through proteinase-inhibitory assay, xylene-induced ear edema test, cotton pellet-induced granuloma formation in mice, formalin test, acetic acid-induced writhing test, tail immersion test and Brewer’s yeastinduced pyrexia in mice.RESULTS： M. paniculata extracts revealed the presence of carbohydrates, alkaloids, saponins, tannins, flavonoids and triterpenoids. All of the extracts showed significant（P〈0.05, vs aspirin group） proteinaseinhibitory activity, whereas the highest effect elicited by plant extracts was exhibited by the BME（75.94% proteinase inhibition activity） with a half-maximal inhibitory concentration（IC50） of 61.31 μg/m L. Each extract at the doses of 200 and 400 mg/kg body weight showed significant（P〈0.05, vs control） percentage inhibition of ear edema and granuloma formation. These extracts significantly（P〈0.05, vs control） reduced the paw licking and abdominal writhing of mice. In addition, BME 400 mg/kg, and FME at 200 and 400 mg/kg showed significant（P〈0.05, vs control） analgesic activities at 60 min in the tail immersion test. Again, the significant（P〈0.05, vs control） post-treatment antipyretic activities were found by BME 200 and 400 mg/kg and FME 400 mg/kg respectively.CONCLUSION： Study results indicate that M. paniculata may provide a source of plant compounds with anti-inflammatory, analgesic and antipyretic activities.
基金supported by Centre for Biomedical Research,University of Dhaka and Department of Applied Chemistry＆Chemical Technology,Islamic University,Kushtia,Bangladesh
文摘OBJECTIVE To isolate and characterize bioactive compound from traditionally important medicinal plant Mikania cordata,and to investigate muti-faced pharmacological activities of the isolated compound.METHODS A triterpeenoid,16-hydroxy betulinic acid(16 HBA) was isolated from Mikania cordata leaf and the structure of the compound was determined by NMR spectroscopic means.Antimicrobial activity of 16 HBA was tested by disc diffusion method and minimum inhibitory concentration(MIC) against the tested microorganisms was determined.The analgesic property of 16 HBA was tested using acetic acid-induced writhes in mice and hot plate thermal stimulation in rats.The anti-inflammatory activity was studied using carrageenin-induced paw edema method.The antipyretic potential of 16 HBA was evaluated by using yeast-induced hyperthermia in rats.RESULTS The triterpenoid 16 HBA showed potent antibacterial activity with inhibition zone of diameter ranging from 12.0~17.5 mm and antifungal activity with mycelial growth inhibition ranges from 37.6%~54.5%.The MIC values for antibacterial and antifungal activities ranged from 31.5~125 and 250~1000 μg·mL-1 respectively.The compound(50 and100 mg·kg^(-1) body weight) showed potent peripheral and central analgesic activity having 55.19% and41% of writhing inhibition at 90 min after administration of the compound and the highest 55.98%,79.18% elongation of reaction time,respectively.In anti-inflammatory activity screening,the compound(100 mg·kg^(-1)) revealed the highest 77.08% edema inhibition at 4 h after administration of carrageenan.In antipyretic assay,16 HBA exhibited a strong antipyretic effect in yeast-induced rats.CONCLUSION The present study confirmed that 16-hydroxy betulinic acid isolated from Mikania cordata has potent antimicrobial,analgesic,anti-inflammatory and antipyretic properties.Our results can be seen as scientific support for the traditional and folklore usage of Mikania cordata in Bangladesh for the treatment of different ailments and provide opportunities to explore this plant as a source bioactive compounds for biochemical and pharmaceutical industries.
