Objective： To evaluate the inhibitory effects of PIN1 antiseuse gene on the proliferation of htnnan osteosarcoma cells. Methods： Different doses of antisense PIN1 gene （0,20,50,100,200,250μl） were transfected int...Objective： To evaluate the inhibitory effects of PIN1 antiseuse gene on the proliferation of htnnan osteosarcoma cells. Methods： Different doses of antisense PIN1 gene （0,20,50,100,200,250μl） were transfected into osteosarcoma MG-63 cells. The cells and the culture supernatants before and after transfection were collected. The cell growth curve was made using MTT method. The cell growth cycle and apoptosis were detected by FCM. The expression of PIN1 was detected by Western blot. The expression of PIN1 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）. Results： MTT and FCM assays indicated that the transfection of antisense PIN1 gene could inhibit profiferation of MG-63 cells and lead to cell apoptosis. Western-blot assays revealed the MG-63 cells transfected with antisense PIN1 gene had weaker expression than those without transfection with antisense PIN1 gene, and the band intensity was negatively related with doses. The cells transfected with different doses of gene （0,20,50,100,200,250μl） had different absobance rate（0.854±0.136,0.866±0.138,0.732±0.154,0.611 ± 0.121, 0. 547 ± 0. 109, 0. 398 ± 0.113, 0. 320 ± 0.151）, with significant difference assessed by F and q test （P〈0.05）. The absorbance rate of PINI mRNA was 0.983±0.125,0.988±0.127, 0.915±0.157, 0.786 ± 0.125,0.608 ± 0.124,0.433 ± 0.130,0.410 ± 0. 158 respectively （ P 〈 0.05）. Conclusion. The expression of PIN1 mRNA in MG-63 cells could be inhibited by antiseuse PIN1 gene, and then the expression of PIN1 was reduced and depressed, and so the proliferation of hmnan osteosarcoma cells MG-63 was inhibited.展开更多
文摘Objective： To evaluate the inhibitory effects of PIN1 antiseuse gene on the proliferation of htnnan osteosarcoma cells. Methods： Different doses of antisense PIN1 gene （0,20,50,100,200,250μl） were transfected into osteosarcoma MG-63 cells. The cells and the culture supernatants before and after transfection were collected. The cell growth curve was made using MTT method. The cell growth cycle and apoptosis were detected by FCM. The expression of PIN1 was detected by Western blot. The expression of PIN1 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）. Results： MTT and FCM assays indicated that the transfection of antisense PIN1 gene could inhibit profiferation of MG-63 cells and lead to cell apoptosis. Western-blot assays revealed the MG-63 cells transfected with antisense PIN1 gene had weaker expression than those without transfection with antisense PIN1 gene, and the band intensity was negatively related with doses. The cells transfected with different doses of gene （0,20,50,100,200,250μl） had different absobance rate（0.854±0.136,0.866±0.138,0.732±0.154,0.611 ± 0.121, 0. 547 ± 0. 109, 0. 398 ± 0.113, 0. 320 ± 0.151）, with significant difference assessed by F and q test （P〈0.05）. The absorbance rate of PINI mRNA was 0.983±0.125,0.988±0.127, 0.915±0.157, 0.786 ± 0.125,0.608 ± 0.124,0.433 ± 0.130,0.410 ± 0. 158 respectively （ P 〈 0.05）. Conclusion. The expression of PIN1 mRNA in MG-63 cells could be inhibited by antiseuse PIN1 gene, and then the expression of PIN1 was reduced and depressed, and so the proliferation of hmnan osteosarcoma cells MG-63 was inhibited.
