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Integrative cytology and proteome alterations unravel the multitarget effect of a novel alkaloid antofine against Penicillium italicum
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作者 彭旋 万春鹏 +4 位作者 张雅婕 陈金印 杨志成 张欣雨 陈楚英 《Food Quality and Safety》 SCIE CSCD 2023年第2期370-382,共13页
Penicillium italicum is the causal agent of citrus blue mold,which is a major threat to the global citrus fruit industry.Antofine,a natural phenanthroindolizidine alkaloid,is water-soluble and exhibits a broad range o... Penicillium italicum is the causal agent of citrus blue mold,which is a major threat to the global citrus fruit industry.Antofine,a natural phenanthroindolizidine alkaloid,is water-soluble and exhibits a broad range of biological activities.However,whether it can inhibit P italicum growth and the potential inhibitory mechanism remains to be elucidated.This study aimed to investigate the antifungal mechanism of antofine against P italicum using scanning electron microscopy,transmission electron microscopy(TEM),propidium iodide staining,and tandem mass tag-labeled quantitative proteomic analysis.Antofine was found to exhibit its preeminent antifungal activity against P italicum with a minimum inhibitory concentration of 1.56 mg/L and a minimum fungicidal concentration of 6.25 mg/L.The challenge test revealed that antofine inhibited the development of citrus blue mold during a 6-d P italicum-infected period.Antofine acted on its potential multitargets to inhibit P italicum growth by synergistically activating oxidative stress through accumulating excess reactive oxygen species,impairing membrane integrity.inducing membrane lipid peroxidation,and disrupting mitochondrial function,thereby disrupting the membrane system and reducing cell via-bility.Moreover,antofine treatment downregulated most differentially expressed proteins involved in carbon metabolism,pyruvate metabolism,and the tricarboxylic acid cycle(TCA)in P italicum mycelia,which may explain the mitochondrial decomposition observed by TEM and the de-clines in ATP levels as well as the activities of TCA-related enzymes.These results indicate that antofine treatment inhibited P italicum growth by targeting the cell membrane and mitochondria. 展开更多
关键词 antofine Penicillium italicum citrus fruit antifungal mechanism quantitative proteomics
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牛心朴子草对烟草病毒病的防效试验 被引量:3
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作者 周德海 宋玉川 +1 位作者 王玉军 朱常香 《山东农业科学》 2007年第6期76-78,共3页
采用室内枯斑寄主法和田间生测试验,测定了植物源Antofine抗病毒水剂——牛心朴子草W制剂对烟草病毒病的防治效果。室内测定结果显示:将牛心朴子草W制剂与病毒汁液混合后接种,对病毒的抑制效果为70%-80%;先喷制剂,24 h后再接种病毒,对... 采用室内枯斑寄主法和田间生测试验,测定了植物源Antofine抗病毒水剂——牛心朴子草W制剂对烟草病毒病的防治效果。室内测定结果显示:将牛心朴子草W制剂与病毒汁液混合后接种,对病毒的抑制效果为70%-80%;先喷制剂,24 h后再接种病毒,对病毒抑制效果为40%-50%;先接种病毒,2 h后再喷制剂,基本无抑制效果。田间对烟草病毒病的防治效果显示:牛心朴子草W制剂对PVY、CMV和TMV的综合防治效果为40%-50%,与目前生产上用的常规药剂病毒必克和菌克毒克防效相近。 展开更多
关键词 植物源antofine抗病毒水剂 牛心朴子草W制剂 病毒病防效
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