Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation. Methods:The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by lip...Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation. Methods:The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by liposome mediation for transient expression. After AP-2α transfected SW620 cells, the exogenous AP-2α mRNA and protein express were determined by the method of Real-time PCR and Western blot. In order to elucidate the effect of expression of exogenous AP-2α gene on the colorectal cancer cell SW620, the proliferation rates were analyzed by growth curves for cells including SW620, pcDNA3.1(+)/SW620 and pcDNA3.1(+)-AP-2α/SW620. At same time, the apoptotic rate of cells and the cell cycle analysis were also tested by flow cytometry. Results: The mRNA and protein expressions of AP-2α gene could be enhanced by transfecting of pcDNA3.1(+)-AP-2α recombinant plasmid into SW620 cell. The cell growth rates of SW620 cells transfected with pcDNA3.1(+)/AP-2α were slower than those transfected with pcDNA3.1(+) or untransfected. The apoptotic rates were increased compared with pcDNA3.1(+)/SW620 and SW620 (P<0.05), which indicated that over-expression of AP-2α gene could efficiently inhibit the growth of SW620 cells and induce apoptosis. FCM analyses indicated that the cells being arrested in G1 phase. Conclusion: AP-2α gene can be efficiently transfected into SW620 cells and over-expression AP-2α protein in the transfected cells. AP-2α induces G1 arrest and apoptosis, suppressive effect on cell growth.展开更多
Objective: To investigate the correlation of AP-2 with trophoblast apoptosis and invasion in placental tissues of preeclampsia. Methods: A total of 68 puerperae with preeclampsia who gave birth in our hospital between...Objective: To investigate the correlation of AP-2 with trophoblast apoptosis and invasion in placental tissues of preeclampsia. Methods: A total of 68 puerperae with preeclampsia who gave birth in our hospital between November 2015 and October 2017 were selected as preeclampsia group, and 60 healthy puerperae who gave birth in our hospital during the same period were selected as normal control group. The differences in expression of AP-2 gene, apoptosis-related genes and invasion-related genes in placenta tissue were compared between the two groups, and the inner link of AP-2 gene expression with trophoblast apoptosis and invasion activity in placental tissues of preeclampsia was further analyzed. Results: AP-2 mRNA expression in placental tissue of preeclampsia group was higher than that of control group;apoptosis-related genes Bcl and Cx43 mRNA expression were lower than those of control group whereas Bax, Caspase7 and metastin mRNA expression were higher than those of control group;invasion-related genes HIF-1α, GPR30, MEST and Snail mRNA expression were lower than those of control group whereas PEDF and RECK mRNA expression were higher than those of control group. Pearson test showed that the AP-2 gene expression in the placental tissues of preeclampsia was directly correlated with the expression of trophoblast apoptosis-related genes and invasion-related genes. Conclusion: AP-2 gene expression significantly increases in the placental tissue of puerperae with preeclampsia, and it can positively regulate the trophoblast apoptosis activity and negatively regulate its invasion activity.展开更多
Objective: To study the correlation of AP-4 and EZH2 gene expression with apoptosis and epithelial-mesenchymal transition in endometrial cancer lesions. Methods: Patients with endometrial cancer who received surgical ...Objective: To study the correlation of AP-4 and EZH2 gene expression with apoptosis and epithelial-mesenchymal transition in endometrial cancer lesions. Methods: Patients with endometrial cancer who received surgical resection in Xiaogan First People's Hospital between February 2015 and January 2017 were selected, right amount of endometrial cancer lesion and adjacent lesion was collected to extract RNA, and then the expression of AP-4 and EZH2 gene as well as apoptosis genes and epithelial-mesenchymal transition genes were determined. Results: AP-4 and EZH2 gene mRNA expression in endothelial cancer lesion were greatly higher than those in adjacent lesion;SRPX2, RLIP76, ZEB1, SALL4, TET1, RANKL and N-cadherin mRNA expression in endometrial cancer lesion were greatly higher than those in adjacent lesions whereas Fat-1, ITLN-1, Caspase-3 and -catenin mRNA expression were greatly lower than those in adjacent lesions;SRPX2 and RLIP76 mRNA expression in endometrial cancer lesion with high AP-4 expression were greatly higher than those in endometrial cancer lesion with low AP-4 expression whereas Fat-1, ITLN-1 and Caspase-3 mRNA expression were greatly lower than those in endometrial cancer lesion with low AP-4 expression;ZEB1, SALL4, TET1, RANKL and N-cadherin mRNA expression in endometrial cancer lesion with high EZH2 expression were greatly higher than those in endometrial cancer lesion with low EZH2 expression whereas α-catenin mRNA expression was greatly lower than that in endometrial cancer lesion with low EZH2 expression. Conclusion: The AP-4 and EZH2 gene highly expressed in endometrial cancer lesion can inhibit the apoptosis of cancer cells and promote the epithelial-mesenchymal transition of cancer cells.展开更多
Prostaglandin J2 (PG J2) and its metabolites are naturally occurring derivatives of Prostaglandin D2 (PG D2). The pathway for formation of these compounds involves sequential conversion of PG D2 to PG J2, and 15-deoxy...Prostaglandin J2 (PG J2) and its metabolites are naturally occurring derivatives of Prostaglandin D2 (PG D2). The pathway for formation of these compounds involves sequential conversion of PG D2 to PG J2, and 15-deoxy-△12,14-prostaglandin-J2 (15d-PGJ2). 15d-PGJ2 is a high-affinity ligand for peroxisome proliferation-activated receptor gamma (PPAR7), it represses several genes in different cell lines. Our previous studies have demonstrated that 15d-PGJ2 induced apoptosis in ECV304 endothelial cells. However, the mechanism remains unclear. In this paper, our aim was to explore the molecular mechanism of 15d-PGJ2 on apoptosis in ECV304 endothelial cells. Hoechst 33258 staining, terminal deoxynucleotidyl transferase-展开更多
文摘Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation. Methods:The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by liposome mediation for transient expression. After AP-2α transfected SW620 cells, the exogenous AP-2α mRNA and protein express were determined by the method of Real-time PCR and Western blot. In order to elucidate the effect of expression of exogenous AP-2α gene on the colorectal cancer cell SW620, the proliferation rates were analyzed by growth curves for cells including SW620, pcDNA3.1(+)/SW620 and pcDNA3.1(+)-AP-2α/SW620. At same time, the apoptotic rate of cells and the cell cycle analysis were also tested by flow cytometry. Results: The mRNA and protein expressions of AP-2α gene could be enhanced by transfecting of pcDNA3.1(+)-AP-2α recombinant plasmid into SW620 cell. The cell growth rates of SW620 cells transfected with pcDNA3.1(+)/AP-2α were slower than those transfected with pcDNA3.1(+) or untransfected. The apoptotic rates were increased compared with pcDNA3.1(+)/SW620 and SW620 (P<0.05), which indicated that over-expression of AP-2α gene could efficiently inhibit the growth of SW620 cells and induce apoptosis. FCM analyses indicated that the cells being arrested in G1 phase. Conclusion: AP-2α gene can be efficiently transfected into SW620 cells and over-expression AP-2α protein in the transfected cells. AP-2α induces G1 arrest and apoptosis, suppressive effect on cell growth.
文摘Objective: To investigate the correlation of AP-2 with trophoblast apoptosis and invasion in placental tissues of preeclampsia. Methods: A total of 68 puerperae with preeclampsia who gave birth in our hospital between November 2015 and October 2017 were selected as preeclampsia group, and 60 healthy puerperae who gave birth in our hospital during the same period were selected as normal control group. The differences in expression of AP-2 gene, apoptosis-related genes and invasion-related genes in placenta tissue were compared between the two groups, and the inner link of AP-2 gene expression with trophoblast apoptosis and invasion activity in placental tissues of preeclampsia was further analyzed. Results: AP-2 mRNA expression in placental tissue of preeclampsia group was higher than that of control group;apoptosis-related genes Bcl and Cx43 mRNA expression were lower than those of control group whereas Bax, Caspase7 and metastin mRNA expression were higher than those of control group;invasion-related genes HIF-1α, GPR30, MEST and Snail mRNA expression were lower than those of control group whereas PEDF and RECK mRNA expression were higher than those of control group. Pearson test showed that the AP-2 gene expression in the placental tissues of preeclampsia was directly correlated with the expression of trophoblast apoptosis-related genes and invasion-related genes. Conclusion: AP-2 gene expression significantly increases in the placental tissue of puerperae with preeclampsia, and it can positively regulate the trophoblast apoptosis activity and negatively regulate its invasion activity.
文摘Objective: To study the correlation of AP-4 and EZH2 gene expression with apoptosis and epithelial-mesenchymal transition in endometrial cancer lesions. Methods: Patients with endometrial cancer who received surgical resection in Xiaogan First People's Hospital between February 2015 and January 2017 were selected, right amount of endometrial cancer lesion and adjacent lesion was collected to extract RNA, and then the expression of AP-4 and EZH2 gene as well as apoptosis genes and epithelial-mesenchymal transition genes were determined. Results: AP-4 and EZH2 gene mRNA expression in endothelial cancer lesion were greatly higher than those in adjacent lesion;SRPX2, RLIP76, ZEB1, SALL4, TET1, RANKL and N-cadherin mRNA expression in endometrial cancer lesion were greatly higher than those in adjacent lesions whereas Fat-1, ITLN-1, Caspase-3 and -catenin mRNA expression were greatly lower than those in adjacent lesions;SRPX2 and RLIP76 mRNA expression in endometrial cancer lesion with high AP-4 expression were greatly higher than those in endometrial cancer lesion with low AP-4 expression whereas Fat-1, ITLN-1 and Caspase-3 mRNA expression were greatly lower than those in endometrial cancer lesion with low AP-4 expression;ZEB1, SALL4, TET1, RANKL and N-cadherin mRNA expression in endometrial cancer lesion with high EZH2 expression were greatly higher than those in endometrial cancer lesion with low EZH2 expression whereas α-catenin mRNA expression was greatly lower than that in endometrial cancer lesion with low EZH2 expression. Conclusion: The AP-4 and EZH2 gene highly expressed in endometrial cancer lesion can inhibit the apoptosis of cancer cells and promote the epithelial-mesenchymal transition of cancer cells.
文摘Prostaglandin J2 (PG J2) and its metabolites are naturally occurring derivatives of Prostaglandin D2 (PG D2). The pathway for formation of these compounds involves sequential conversion of PG D2 to PG J2, and 15-deoxy-△12,14-prostaglandin-J2 (15d-PGJ2). 15d-PGJ2 is a high-affinity ligand for peroxisome proliferation-activated receptor gamma (PPAR7), it represses several genes in different cell lines. Our previous studies have demonstrated that 15d-PGJ2 induced apoptosis in ECV304 endothelial cells. However, the mechanism remains unclear. In this paper, our aim was to explore the molecular mechanism of 15d-PGJ2 on apoptosis in ECV304 endothelial cells. Hoechst 33258 staining, terminal deoxynucleotidyl transferase-
