The arginine-glycine-aspartic （RGD） acid peptide was grafted to the surface of apatitewollastonite （AW） ceramic in an effort to improve its cell adhesion, proliferation and osteoinduction. RGD peptide was covalent...The arginine-glycine-aspartic （RGD） acid peptide was grafted to the surface of apatitewollastonite （AW） ceramic in an effort to improve its cell adhesion, proliferation and osteoinduction. RGD peptide was covalently immobilized onto the surface of AW ceramic via the synthetic cross linker AA.PTS-E and 1-ethyl-3-（3-dimethylaminopropyl）carbodiimide （EDC）. The modified surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy （ATR-FTIR） and X-ray photoelectron spectroscopy （XPS）. The chemical analysis indicated that RGD peptide had been immobilized onto the AW surface successfully. The growth of osteoblast-like cells （MG63） showed that modifying the AW surface with RGD peptide enhanced the cell adhesion and proliferation. And the histological evaluation of RGD-AW showed that the bone regeneration and remodeling process were significantly enhanced compared to the original AW ceramics after 2, 4 and 8 weeks implantation in rabbit＇s femoral condyles.展开更多
In this study, lysine was introduced into the surface of apatite-wollastonite glass ceramic （AW-GC） to improve its cytocompatibility by two steps reaction procedure. Firstly, lysine connected to N-β-（aminoethyl）-...In this study, lysine was introduced into the surface of apatite-wollastonite glass ceramic （AW-GC） to improve its cytocompatibility by two steps reaction procedure. Firstly, lysine connected to N-β-（aminoethyl）-y-aminopropyl trimethoxy silane （A-1120） by covalent binding of amide group. Secondly, the lysine-functionalized A-1120 was deposited on the surface of AW-GC through a silanization reaction involving a covalent attachment. FTIR spectra indicated that lysine had been immobilized onto the surface of AW-GC successfully. Bioactivity of the surface modified AW-GC was investigated by simulated body fluid （SBF）, and the in vitro cytocompatibility was evaluated by coculturing with human osteosarcoma cell MG63. The results showed that the process of hydroxyapatite layer formed on the modified material was similar to AW-GC while the mode of hydroxyapatite deposition was changed. The growth of MG63 cells showed that modifying the AW-GC surface with lysine enhances the cell adhesion and proliferation.展开更多
基金Supported in part by the Specialized Research Fund for theDoctoral Program of Higher Education of China(No.20060610024)
文摘The arginine-glycine-aspartic （RGD） acid peptide was grafted to the surface of apatitewollastonite （AW） ceramic in an effort to improve its cell adhesion, proliferation and osteoinduction. RGD peptide was covalently immobilized onto the surface of AW ceramic via the synthetic cross linker AA.PTS-E and 1-ethyl-3-（3-dimethylaminopropyl）carbodiimide （EDC）. The modified surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy （ATR-FTIR） and X-ray photoelectron spectroscopy （XPS）. The chemical analysis indicated that RGD peptide had been immobilized onto the AW surface successfully. The growth of osteoblast-like cells （MG63） showed that modifying the AW surface with RGD peptide enhanced the cell adhesion and proliferation. And the histological evaluation of RGD-AW showed that the bone regeneration and remodeling process were significantly enhanced compared to the original AW ceramics after 2, 4 and 8 weeks implantation in rabbit＇s femoral condyles.
文摘In this study, lysine was introduced into the surface of apatite-wollastonite glass ceramic （AW-GC） to improve its cytocompatibility by two steps reaction procedure. Firstly, lysine connected to N-β-（aminoethyl）-y-aminopropyl trimethoxy silane （A-1120） by covalent binding of amide group. Secondly, the lysine-functionalized A-1120 was deposited on the surface of AW-GC through a silanization reaction involving a covalent attachment. FTIR spectra indicated that lysine had been immobilized onto the surface of AW-GC successfully. Bioactivity of the surface modified AW-GC was investigated by simulated body fluid （SBF）, and the in vitro cytocompatibility was evaluated by coculturing with human osteosarcoma cell MG63. The results showed that the process of hydroxyapatite layer formed on the modified material was similar to AW-GC while the mode of hydroxyapatite deposition was changed. The growth of MG63 cells showed that modifying the AW-GC surface with lysine enhances the cell adhesion and proliferation.
