Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The pr...Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15 -89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol (TC),TG,high-density lipoprotein cholesterol(HDL-C), ApoA1 and ApoB were higher in drinkers than in nondrinkers (P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs) were not different between the two groups.The levels of TG in non-drinkers, and TC,TG,low-density lipoprotein cholesterol (LDL-C)and ApoB in drinkers were different among the three -1131T】C genotypes(P【0.05-0.001).The -1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all), but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types (P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T, G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of all haplotypes in the both populations.The ApoA5 haplotypes were significantly(P【0.05) associated at the global level with TC,TG,HDL-C, LDL-C,Apo1,and ApoB,even after correction for multiple testing with permutation test.In particular,carriers of haplo-type G-G-C had significantly higher TC,TG,LDL-C,ApoB than noncarriers,whereas carriers of haplotype C-A-T had significantly lower TC,LDL-C,ApoAl and ApoB,and higher HDL-C than noncarriers.Serum TC levels in nondrinkers were correlated with -1131T】C genotype and allele(P【0.05 for each),whereas serum TC,TG and LDL-C levels in drinkers were associated with -1131 T】C and C.553G】T genotypes,or c.457G】A alleles(P【0.05-0.001).Serum lipid parameters were also correlated with several environmental factors in the both groups.Conclusions The differences in serum lipid profiles between the drinkers and nondrinkers might partly result from different interactions of ApoA5 gene polymor phisms and alcohol consumption.genotypes and -1131T】C, c.553G】T and c.457G】A to detect the interactions of the ApoA5展开更多
Objectives Both apolipoprotein(Apo)C-Ⅲgene polymorphism and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but their interactions on serum TG levels are not well known.The prese...Objectives Both apolipoprotein(Apo)C-Ⅲgene polymorphism and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but their interactions on serum TG levels are not well known.The present study was undertaken to detect the interactions of the ApoC-Ⅲ3238C】G(rs5128) polymorphism and alcohol consumption on serum TG levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15-89 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the ApoC-Ⅲ3238C】G was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Interactions of the ApoC-Ⅲ3238C】G genotype and alcohol consumption was assessed by using a cross-product term between genotypes and the afore-mentioned factor.Results Serum total cholesterol(TC), TG,high-density lipoprotein cholesterol(HDL-C),ApoA-I and ApoB levels were higher in drinkers than in nondrinkers (P【0.05-0.001).There was no significant difference in the genotypic and allelic frequencies between the two groups. Serum TG levels in nondrinkers were higher in CG genotype than in CC genotype(P【0,01).Serum TC,TG,low-density lipoprotein cholesterol(LDL-C) and ApoB levels in drinkers were higher in GG genotype than in CC or CG genotype(P【0.01 for all).Serum HDL-C levels in drinkers were higher in CG genotype than in CC genotype(P【0.01).Serum TC, TG,HDL-C and ApoA-I levels in CC genotype,TC,HDL-C, ApoA-I levels and the ratio of ApoA-I to ApoB in CG genotype, and TC,TG,LDL-C,ApoA-I and ApoB levels in GG genotype were higher in drinkers than in nondrinkers(P【0.05-0.01).But the ratio of ApoA-I to ApoB in GG genotype was lower in drinkers than in nondrinkers(P【0.01). Multivariate logistic regression analysis showed that the levels of TC,TG and ApoB were correlated with genotype in non drinkers(P【0.05 for all).The levels of TC,LDL-C and ApoB were associated with genotype in drinkers(P【0.01 for all). Serum lipid parameters were also correlated with age,sex,alcohol consumption,cigarette smoking,blood pressure,body weight,and body mass index in both groups.Conclusions This study suggests that the ApoC-Ⅲ3238CG heterozygotes benefited more from alcohol consumption than CC and GG homozygotes in increasing serum levels of HDL-C,ApoA-I, and the ratio of ApoA-I to ApoB,and lowering serum levels of TC and TG.展开更多
文摘Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15 -89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol (TC),TG,high-density lipoprotein cholesterol(HDL-C), ApoA1 and ApoB were higher in drinkers than in nondrinkers (P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs) were not different between the two groups.The levels of TG in non-drinkers, and TC,TG,low-density lipoprotein cholesterol (LDL-C)and ApoB in drinkers were different among the three -1131T】C genotypes(P【0.05-0.001).The -1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all), but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types (P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T, G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of all haplotypes in the both populations.The ApoA5 haplotypes were significantly(P【0.05) associated at the global level with TC,TG,HDL-C, LDL-C,Apo1,and ApoB,even after correction for multiple testing with permutation test.In particular,carriers of haplo-type G-G-C had significantly higher TC,TG,LDL-C,ApoB than noncarriers,whereas carriers of haplotype C-A-T had significantly lower TC,LDL-C,ApoAl and ApoB,and higher HDL-C than noncarriers.Serum TC levels in nondrinkers were correlated with -1131T】C genotype and allele(P【0.05 for each),whereas serum TC,TG and LDL-C levels in drinkers were associated with -1131 T】C and C.553G】T genotypes,or c.457G】A alleles(P【0.05-0.001).Serum lipid parameters were also correlated with several environmental factors in the both groups.Conclusions The differences in serum lipid profiles between the drinkers and nondrinkers might partly result from different interactions of ApoA5 gene polymor phisms and alcohol consumption.genotypes and -1131T】C, c.553G】T and c.457G】A to detect the interactions of the ApoA5
文摘Objectives Both apolipoprotein(Apo)C-Ⅲgene polymorphism and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but their interactions on serum TG levels are not well known.The present study was undertaken to detect the interactions of the ApoC-Ⅲ3238C】G(rs5128) polymorphism and alcohol consumption on serum TG levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15-89 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the ApoC-Ⅲ3238C】G was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Interactions of the ApoC-Ⅲ3238C】G genotype and alcohol consumption was assessed by using a cross-product term between genotypes and the afore-mentioned factor.Results Serum total cholesterol(TC), TG,high-density lipoprotein cholesterol(HDL-C),ApoA-I and ApoB levels were higher in drinkers than in nondrinkers (P【0.05-0.001).There was no significant difference in the genotypic and allelic frequencies between the two groups. Serum TG levels in nondrinkers were higher in CG genotype than in CC genotype(P【0,01).Serum TC,TG,low-density lipoprotein cholesterol(LDL-C) and ApoB levels in drinkers were higher in GG genotype than in CC or CG genotype(P【0.01 for all).Serum HDL-C levels in drinkers were higher in CG genotype than in CC genotype(P【0.01).Serum TC, TG,HDL-C and ApoA-I levels in CC genotype,TC,HDL-C, ApoA-I levels and the ratio of ApoA-I to ApoB in CG genotype, and TC,TG,LDL-C,ApoA-I and ApoB levels in GG genotype were higher in drinkers than in nondrinkers(P【0.05-0.01).But the ratio of ApoA-I to ApoB in GG genotype was lower in drinkers than in nondrinkers(P【0.01). Multivariate logistic regression analysis showed that the levels of TC,TG and ApoB were correlated with genotype in non drinkers(P【0.05 for all).The levels of TC,LDL-C and ApoB were associated with genotype in drinkers(P【0.01 for all). Serum lipid parameters were also correlated with age,sex,alcohol consumption,cigarette smoking,blood pressure,body weight,and body mass index in both groups.Conclusions This study suggests that the ApoC-Ⅲ3238CG heterozygotes benefited more from alcohol consumption than CC and GG homozygotes in increasing serum levels of HDL-C,ApoA-I, and the ratio of ApoA-I to ApoB,and lowering serum levels of TC and TG.
