Objective:To investigate the effect of adjuvant salvianolate therapy on plaque stability, cell apoptosis and coagulation indexes in patients with unstable angina pectoris.Methods: 92 patients with unstable angina pect...Objective:To investigate the effect of adjuvant salvianolate therapy on plaque stability, cell apoptosis and coagulation indexes in patients with unstable angina pectoris.Methods: 92 patients with unstable angina pectoris treated in our hospital between May 2011 and August 2015 were collected, and after the treatment process and auxiliary examination results were retrospectively analyzed, they were divided into the control group (n=45) who accepted conventional treatment and the observation group (n=47) who accepted adjuvant salvianolate treatment. Before and after treatment, diasonograph was used to evaluate the plaque stability parameters of two groups of patients;ELISA was used to detect apoptosis-related molecule levels;immunoturbidimetry was used to detect blood coagulation indexes.Results: Before treatment, differences in plaque stability parameters, cell apoptosis molecules and coagulation indexes were not statistically significant between two groups of patients (P>0.05). After treatment, the plaque stability parameters plaque thickness, enhanced intensity, rise time and time to peak of observation group were significantly lower than those of control group (P<0.05);serum sFas, sFasL, fibrinogen (Fib), platelet (PLT), and D-Dimer (D-D) levels of observation group were significantly lower than those of control group while Bcl-2, prothrombin time (PT) and activated partial thromboplastin time (APTT) levels were significantly higher than those of control group (P<0.05).Conclusions: Adjuvant salvianolate treatment can increase the plaque stability, also inhibit myocardial cell apoptosis and improve the coagulation function in patients with unstable angina pectoris.展开更多
Aquaporin 5 has been recently found as an important oncogenic marker whose expression levels seem to be determined by the level of cellular differentiation. Despite aquaporin volume decrease (AVD) being the most conse...Aquaporin 5 has been recently found as an important oncogenic marker whose expression levels seem to be determined by the level of cellular differentiation. Despite aquaporin volume decrease (AVD) being the most conserved earliest event in apoptosis, there is still a paucity of studies exploring on aquaporin expression and its relationship with apoptosis in cancer. The aim of this study was to investigate the expression of aquaporin 5 channel protein and to explore on its relationship with apoptosis in well and poorly differentiated non-small cell lung carcinoma both in-vivo and in-vitro. Findings from the study showed that the expression of AQP5 both in-vivo and in-vitro was dependent on the type and degree of tumour differentiation. In-vivo, an increase in aquaporin 5 expression was associated with an increased apoptosis in both poorly and highly differentiated adenocarcinoma (AC) while there was no association between aquaporin 5 expression and apoptosis in both poorly and highly differentiated squamous cell carcinoma (SCC). In vitro, differentiation therapy in the form of ATRA decreased both cell proliferation and increased the expression of AQP5 in A549 cells. The cytomorphological changes, expression of differentiation markers and flow cytometry apoptotic results were dependent on the dose of ATRA treatment. In conclusion, a higher expression of aquaporin 5 was found to promote the rate of the apoptotic process in lung adenocarcinoma (AC).展开更多
Treatment-induced apoptosis of cancer cells is one goal of cancer therapy.Interestingly,more heat is generated by mitochondria during apoptosis,especially the uncoupled apoptotic state,^(1,2) compared to the resting s...Treatment-induced apoptosis of cancer cells is one goal of cancer therapy.Interestingly,more heat is generated by mitochondria during apoptosis,especially the uncoupled apoptotic state,^(1,2) compared to the resting state.In this case study,we explore these thermal effects by longitudinally measuring temperature variations in a breast lesion of a pathological complete responder during neoadjuvant chemotherapy(NAC).Diffuse Optical Spectroscopic Imaging(DOSI)was employed to derive absolute deep tissue temperature using subtle spectral features of the water peak at 975 nm.^(3)A significant temperature increase was observed in time windows during the anthracycline and cyclophosphamide(AC)regimen but not in the paclitaxel and bevacizumab regimen.Hemoglobin concentration changes generally did not follow temperature,suggesting the measured temperature increases were likely due to mitochondrial uncoupling rather than a direct vascular effect.A simultaneous increase of tissue oxygen saturation with temperature was observed,suggesting that oxidative stress also contributes to apoptosis.Although preliminary,this study indicates longitudinal DOSI tissue temperature monitoring provides information that can improve our understanding of the mechanisms of tissue response during NAC.展开更多
This research extensively evaluates three leading mathematical software packages: Python, MATLAB, and Scilab, in the context of solving nonlinear systems of equations with five unknown variables. The study’s core obj...This research extensively evaluates three leading mathematical software packages: Python, MATLAB, and Scilab, in the context of solving nonlinear systems of equations with five unknown variables. The study’s core objectives include comparing software performance using standardized benchmarks, employing key performance metrics for quantitative assessment, and examining the influence of varying hardware specifications on software efficiency across HP ProBook, HP EliteBook, Dell Inspiron, and Dell Latitude laptops. Results from this investigation reveal insights into the capabilities of these software tools in diverse computing environments. On the HP ProBook, Python consistently outperforms MATLAB in terms of computational time. Python also exhibits a lower robustness index for problems 3 and 5 but matches or surpasses MATLAB for problem 1, for some initial guess values. In contrast, on the HP EliteBook, MATLAB consistently exhibits shorter computational times than Python across all benchmark problems. However, Python maintains a lower robustness index for most problems, except for problem 3, where MATLAB performs better. A notable challenge is Python’s failure to converge for problem 4 with certain initial guess values, while MATLAB succeeds in producing results. Analysis on the Dell Inspiron reveals a split in strengths. Python demonstrates superior computational efficiency for some problems, while MATLAB excels in handling others. This pattern extends to the robustness index, with Python showing lower values for some problems, and MATLAB achieving the lowest indices for other problems. In conclusion, this research offers valuable insights into the comparative performance of Python, MATLAB, and Scilab in solving nonlinear systems of equations. It underscores the importance of considering both software and hardware specifications in real-world applications. The choice between Python and MATLAB can yield distinct advantages depending on the specific problem and computational environment, providing guidance for researchers and practitioners in selecting tools for their unique challenges.展开更多
文摘Objective:To investigate the effect of adjuvant salvianolate therapy on plaque stability, cell apoptosis and coagulation indexes in patients with unstable angina pectoris.Methods: 92 patients with unstable angina pectoris treated in our hospital between May 2011 and August 2015 were collected, and after the treatment process and auxiliary examination results were retrospectively analyzed, they were divided into the control group (n=45) who accepted conventional treatment and the observation group (n=47) who accepted adjuvant salvianolate treatment. Before and after treatment, diasonograph was used to evaluate the plaque stability parameters of two groups of patients;ELISA was used to detect apoptosis-related molecule levels;immunoturbidimetry was used to detect blood coagulation indexes.Results: Before treatment, differences in plaque stability parameters, cell apoptosis molecules and coagulation indexes were not statistically significant between two groups of patients (P>0.05). After treatment, the plaque stability parameters plaque thickness, enhanced intensity, rise time and time to peak of observation group were significantly lower than those of control group (P<0.05);serum sFas, sFasL, fibrinogen (Fib), platelet (PLT), and D-Dimer (D-D) levels of observation group were significantly lower than those of control group while Bcl-2, prothrombin time (PT) and activated partial thromboplastin time (APTT) levels were significantly higher than those of control group (P<0.05).Conclusions: Adjuvant salvianolate treatment can increase the plaque stability, also inhibit myocardial cell apoptosis and improve the coagulation function in patients with unstable angina pectoris.
文摘Aquaporin 5 has been recently found as an important oncogenic marker whose expression levels seem to be determined by the level of cellular differentiation. Despite aquaporin volume decrease (AVD) being the most conserved earliest event in apoptosis, there is still a paucity of studies exploring on aquaporin expression and its relationship with apoptosis in cancer. The aim of this study was to investigate the expression of aquaporin 5 channel protein and to explore on its relationship with apoptosis in well and poorly differentiated non-small cell lung carcinoma both in-vivo and in-vitro. Findings from the study showed that the expression of AQP5 both in-vivo and in-vitro was dependent on the type and degree of tumour differentiation. In-vivo, an increase in aquaporin 5 expression was associated with an increased apoptosis in both poorly and highly differentiated adenocarcinoma (AC) while there was no association between aquaporin 5 expression and apoptosis in both poorly and highly differentiated squamous cell carcinoma (SCC). In vitro, differentiation therapy in the form of ATRA decreased both cell proliferation and increased the expression of AQP5 in A549 cells. The cytomorphological changes, expression of differentiation markers and flow cytometry apoptotic results were dependent on the dose of ATRA treatment. In conclusion, a higher expression of aquaporin 5 was found to promote the rate of the apoptotic process in lung adenocarcinoma (AC).
基金This work was supported by NIH R01-CA75124,R01-EB002109Susan G.Komen for the Cure Postdoctoral Fellowship provided to University of Pennsylvania,and P41-RR01192,U54-CA105480,U54CA136400,P30-CA62203 provided to University of California,Irvine.
文摘Treatment-induced apoptosis of cancer cells is one goal of cancer therapy.Interestingly,more heat is generated by mitochondria during apoptosis,especially the uncoupled apoptotic state,^(1,2) compared to the resting state.In this case study,we explore these thermal effects by longitudinally measuring temperature variations in a breast lesion of a pathological complete responder during neoadjuvant chemotherapy(NAC).Diffuse Optical Spectroscopic Imaging(DOSI)was employed to derive absolute deep tissue temperature using subtle spectral features of the water peak at 975 nm.^(3)A significant temperature increase was observed in time windows during the anthracycline and cyclophosphamide(AC)regimen but not in the paclitaxel and bevacizumab regimen.Hemoglobin concentration changes generally did not follow temperature,suggesting the measured temperature increases were likely due to mitochondrial uncoupling rather than a direct vascular effect.A simultaneous increase of tissue oxygen saturation with temperature was observed,suggesting that oxidative stress also contributes to apoptosis.Although preliminary,this study indicates longitudinal DOSI tissue temperature monitoring provides information that can improve our understanding of the mechanisms of tissue response during NAC.
文摘This research extensively evaluates three leading mathematical software packages: Python, MATLAB, and Scilab, in the context of solving nonlinear systems of equations with five unknown variables. The study’s core objectives include comparing software performance using standardized benchmarks, employing key performance metrics for quantitative assessment, and examining the influence of varying hardware specifications on software efficiency across HP ProBook, HP EliteBook, Dell Inspiron, and Dell Latitude laptops. Results from this investigation reveal insights into the capabilities of these software tools in diverse computing environments. On the HP ProBook, Python consistently outperforms MATLAB in terms of computational time. Python also exhibits a lower robustness index for problems 3 and 5 but matches or surpasses MATLAB for problem 1, for some initial guess values. In contrast, on the HP EliteBook, MATLAB consistently exhibits shorter computational times than Python across all benchmark problems. However, Python maintains a lower robustness index for most problems, except for problem 3, where MATLAB performs better. A notable challenge is Python’s failure to converge for problem 4 with certain initial guess values, while MATLAB succeeds in producing results. Analysis on the Dell Inspiron reveals a split in strengths. Python demonstrates superior computational efficiency for some problems, while MATLAB excels in handling others. This pattern extends to the robustness index, with Python showing lower values for some problems, and MATLAB achieving the lowest indices for other problems. In conclusion, this research offers valuable insights into the comparative performance of Python, MATLAB, and Scilab in solving nonlinear systems of equations. It underscores the importance of considering both software and hardware specifications in real-world applications. The choice between Python and MATLAB can yield distinct advantages depending on the specific problem and computational environment, providing guidance for researchers and practitioners in selecting tools for their unique challenges.
