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Partition coefficient prediction of Baker's yeast invertase in aqueous two phase systems using hybrid group method data handling neural network 被引量:1
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作者 Carlos Eduardo de Araújo Padilha Sérgio Dantas de Oliveira Júnior +3 位作者 Domingos Fabiano de Santana Souza Jackson Araújo de Oliveira Gorete Ribeiro de Macedo Everaldo Silvino dos Santos 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2017年第5期652-657,共6页
A hybrid GMDH neural network model has been developed in order to predict the partition coefficients of invertase from Baker's yeast. ATPS experiments were carried out changing the molar average mass of PEG(1500–... A hybrid GMDH neural network model has been developed in order to predict the partition coefficients of invertase from Baker's yeast. ATPS experiments were carried out changing the molar average mass of PEG(1500–6000 Da), p H(4.0–7.0), percentage of PEG(10.0–20.0 w/w), percentage of MgSO_4(8.0–16.0 w/w), percentage of the cell homogenate(10.0–20.0 w/w) and the percentage of MnSO_4(0–5.0 w/w) added as cosolute. The network evaluation was carried out comparing the partition coefficients obtained from the hybrid GMDH neural network with the experimental data using different statistical metrics. The hybrid GMDH neural network model showed better fitting(AARD = 32.752%) as well as good generalization capacity of the partition coefficients of the ATPS than the original GMDH network approach and a BPANN model. Therefore hybrid GMDH neural network model appears as a powerful tool for predicting partition coefficients during downstream processing of biomolecules. 展开更多
关键词 Partitioning Invertase aqueous two phase System GMDH Neural network
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Novel Vesicular Aqueous Two-Phase Systems 被引量:2
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作者 Guo Xi ZHAO Wang Lin YU +1 位作者 Yu Jun GONG Bu Yao ZHU (Institute of Physical Chemistry, Peking University, Beijing 100871) 《Chinese Chemical Letters》 SCIE CAS CSCD 1998年第11期0-0,0-0,共4页
The interesting phenopmena of two aqueous phases coexisting in dilute aqueous solutions of sodium undecenoate-dodecyltrimethylammo brodride ndxture and sodium laurate-dodecyltrimethylammonium brondde mixture were inve... The interesting phenopmena of two aqueous phases coexisting in dilute aqueous solutions of sodium undecenoate-dodecyltrimethylammo brodride ndxture and sodium laurate-dodecyltrimethylammonium brondde mixture were investigated. Vesicles existing in both phases were shown by TEM images. The vesicles are dispersed in lower phase and flocculated in upper phase. Multilamellar structure of vesicles was found in the upper phase of sodium laurate-dodecyltrimethylammonium bromide system. 展开更多
关键词 Vesicular aqueous two phase catanionic surfactants sodium carboxylates alkyltrimethylarnmonium bromide
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Extraction of Phenylalanine Phase Systems Containing Enantiomers by Aqueous Two Combinatorial Chiral Selector 被引量:1
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作者 陈晓青 刘莉 +1 位作者 焦飞鹏 王珍 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2012年第4期965-969,共5页
In order to obtain a better enantioselectivity of phenylalanine enantiomers and establish the optimal chiral ex- traction conditions, the distribution behavior was investigated in aqueous two-phase systems which were ... In order to obtain a better enantioselectivity of phenylalanine enantiomers and establish the optimal chiral ex- traction conditions, the distribution behavior was investigated in aqueous two-phase systems which were composed of polyethylene glycol and ammonium sulfate containing combinatorial chiral selector: β-cyclodextrin and HP-β-cyclodextrin. The influence of the molar concentration ratio of combinatorial chiral selectors, the total molar concentration of combinatorial chiral selectors, pH value, buffer type and its concentration were thoroughly studied, respectively. The results show that the enantioselectivity reaches 1.53 under the optimal chiral extraction conditions This extraction is a potential economical and effective way for chiral resolution. 展开更多
关键词 aqueous two phase extraction combinatorial chiral selectors chiral resolution ENANTIOSELECTIVITY phenylalanine enantiomers
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