The purpose of this research was the bioconversion of biodiesel-derived glycerol to arabitol through the adoption of sustainable bioprocessing.Arabitol production by Debaryomyces prosopidis FMCC Y69 was significantly ...The purpose of this research was the bioconversion of biodiesel-derived glycerol to arabitol through the adoption of sustainable bioprocessing.Arabitol production by Debaryomyces prosopidis FMCC Y69 was significantly affected by initial glycerol concentrations.The highest arabitol concentration of 45.5 g/L was achieved in the case of 120 g/L initial glycerol combined with the highest yield(0.379 g arabitol/g initial glycerol).The yeast strain showed a non-typical oleaginous behavior with low accumulation of lipids(up to 20.9%w/w)despite nitrogen-limited condition that prevailed in the fermentation media.Endopolysaccharides(IPs)were considerably high with a yield on the produced dry cell weight(YIPs/X)(=40.9%w/w)when 85 g/L of glycerol was utilized as the starting material.The fermentation efficiency was further improved when the optimal carbon to nitrogen(C/N)ratio was applied.More specifically,arabitol production was maximized(reaching 56.5 g/L)at a C/N ratio of 158 moles/moles while yield and productivity were also enhanced with respective values of 0.48 g arabitol/g consumed glycerol and 0.112 g/L.h.The increase in C/N ratios led to a decrease in cell dry weight(DCW)concentrations,which is rationally associated with decreased nitrogen availability in the growth medium.YIPs/X values were remarkably high(23.1–26.1%w/w)at the end of each fermentation with the highest concentration in absolute values of 7.3 g/L achieved at the lowest C/N ratio employed(i.e.,79 moles/moles).This study demonstrated the efficient arabitol production along with other value-added metabolites,such as IPs and cellular lipids,implementing biotechnology and renewable resources and thus contributing to the development of circular economy technologies.展开更多
The partial genomic library of Acetobacter suboxydans was constructed using Yeast\| E.coli shuttle plasmid YEp352 as vector.Two positive transformants,designated as DH5α(pAD91) and DH5α(pAD98),were obtained by scree...The partial genomic library of Acetobacter suboxydans was constructed using Yeast\| E.coli shuttle plasmid YEp352 as vector.Two positive transformants,designated as DH5α(pAD91) and DH5α(pAD98),were obtained by screening the growth of transformants on the agar plate in which D\|arabitol was used as the sole carbon source.The results of Southern blot and restriction endonuclease analysis showed that the two recombinants are identical.The insert is about 2.3kb.Arabitol dehydrogenase activity assay indicated that the transformants could produce D\|xylulose\|forming D\|arabitol dehydrogenase.Hence,the gene encoding D\|arabitol dehydrogenase exists in the cloned DNA fragment.展开更多
基金the project entitled“Research Infrastructure for Waste Valorization and Sustainable Management of Resources-INVALOR”(MIS 5002495)which is implemented under the Action“Reinforcement of the Research and Innovation Infrastructure”,funded by the Operational Program“Competitiveness,Entrepreneurship and Innovation”(NSRF 2014-2020)co-financed by Greece and the European Union(European Regional Development Fund).
文摘The purpose of this research was the bioconversion of biodiesel-derived glycerol to arabitol through the adoption of sustainable bioprocessing.Arabitol production by Debaryomyces prosopidis FMCC Y69 was significantly affected by initial glycerol concentrations.The highest arabitol concentration of 45.5 g/L was achieved in the case of 120 g/L initial glycerol combined with the highest yield(0.379 g arabitol/g initial glycerol).The yeast strain showed a non-typical oleaginous behavior with low accumulation of lipids(up to 20.9%w/w)despite nitrogen-limited condition that prevailed in the fermentation media.Endopolysaccharides(IPs)were considerably high with a yield on the produced dry cell weight(YIPs/X)(=40.9%w/w)when 85 g/L of glycerol was utilized as the starting material.The fermentation efficiency was further improved when the optimal carbon to nitrogen(C/N)ratio was applied.More specifically,arabitol production was maximized(reaching 56.5 g/L)at a C/N ratio of 158 moles/moles while yield and productivity were also enhanced with respective values of 0.48 g arabitol/g consumed glycerol and 0.112 g/L.h.The increase in C/N ratios led to a decrease in cell dry weight(DCW)concentrations,which is rationally associated with decreased nitrogen availability in the growth medium.YIPs/X values were remarkably high(23.1–26.1%w/w)at the end of each fermentation with the highest concentration in absolute values of 7.3 g/L achieved at the lowest C/N ratio employed(i.e.,79 moles/moles).This study demonstrated the efficient arabitol production along with other value-added metabolites,such as IPs and cellular lipids,implementing biotechnology and renewable resources and thus contributing to the development of circular economy technologies.
文摘The partial genomic library of Acetobacter suboxydans was constructed using Yeast\| E.coli shuttle plasmid YEp352 as vector.Two positive transformants,designated as DH5α(pAD91) and DH5α(pAD98),were obtained by screening the growth of transformants on the agar plate in which D\|arabitol was used as the sole carbon source.The results of Southern blot and restriction endonuclease analysis showed that the two recombinants are identical.The insert is about 2.3kb.Arabitol dehydrogenase activity assay indicated that the transformants could produce D\|xylulose\|forming D\|arabitol dehydrogenase.Hence,the gene encoding D\|arabitol dehydrogenase exists in the cloned DNA fragment.
