Improved observation of colonized roots reveals the regulation of arbuscule development and senescence by drought stress in the arbuscular mycorrhizae of citrus

Citrus is the typical mycorrhizal fruit tree species establishing symbiosis with arbuscular mycorrhizal (AM) fungi. However, arbuscule development and senescence in colonized citrus roots, especially in response to drought stress, remain unclear, which is mainly due to the difficulty in clearing and staining lignified roots with the conventional method. Here, we improved the observation of colonized roots of citrus plants with the sectioning method, which enabled the clear observation of AM fungal structures. Furthermore, we investigated the effects of one week of drought stress on arbuscule development and senescence with the sectioning method. Microscopy observations indicated that drought stress significantly decreased mycorrhizal colonization (F%and M%) although it did not affect plant growth performance. Fluorescence probes (WGA 488 and/or Nile red) revealed that drought stress inhibited arbuscule development by increasing the percentage of arbuscules at the early stage and decreasing the percentages of arbuscules at the midterm and mature stages. Meanwhile, drought stress accelerated arbuscule senescence, which was characterized by the increased accumulation of neutral lipids. Overall, the sectioning method developed in this study enables the in-depth investigation of arbuscule status, and drought stress can inhibit arbuscule development but accelerate arbuscule senescence in the colonized roots of citrus plants. This study paves the way to elaborately dissecting the arbuscule dynamics in the roots of fruit tree species in response to diverse abiotic stresses.

General and specialized metabolites in peanut roots regulate arbuscular mycorrhizal symbiosis

Arbuscular mycorrhizae(AM)fungi form symbiotic associations with plant roots,providing nutritional benefits and promoting plant growth and defenses against various stresses.Metabolic changes in the roots during AM fungal colonization are key to understanding the development and maintenance of these symbioses.Here,we investigated metabolic changes in the roots of peanut(Arachis hypogaea L.)plants during the colonization and development of AM symbiosis,and compared them to uncolonized roots.The primary changes during the initial stage of AM colonization were in the contents and compositions of phenylpropanoid and flavonoid compounds.These compounds function in signaling pathways that regulate recognition,interactions,and pre-colonization between roots and AM fungi.Flavonoid compounds decreased by 25%when the symbiosis was fully established compared to the initial colonization stage.After AM symbiosis was established,general metabolism strongly shifted toward the formation of lipids,amino acids,carboxylic acids,and carbohydrates.Lipid compounds increased by 8.5%from the pre-symbiotic stage to well-established symbiosis.Lyso-phosphatidylcholines,which are signaling compounds,were only present in AM roots,and decreased in content after the symbiosis was established.In the initial stage of AM establishment,the content of salicylic acid increased two-fold,whereas jasmonic acid and abscisic acid decreased compared to uncolonized roots.The jasmonic acid content decreased in roots after the symbiosis was well established.AM symbiosis was associated with high levels of calcium,magnesium,and D-(+)-mannose,which stimulated seedling growth.Overall,specific metabolites that favor the establishment of AM symbiosis were common in the roots,primarily during early colonization,whereas general metabolism was strongly altered when AM symbiosis was well-established.In conclusion,specialized metabolites function as signaling compounds to establish AM symbiosis.These compounds are no longer produced after the symbiosis between the roots and AM becomes fully established.

Soil Physico-Chemical Properties and Different Altitudes Affect Arbuscular Mycorrhizal Fungi Abundance and Colonization in Cacao Plantations of Cameroon

This study aims to investigate the abundance of AMF according to soil properties and altitudes in different cacao plantations of Cameroon. Physico-chemical analyses were made on soil samples collected from three agro-ecological zones. Soil samples were also used to evaluate directly the AMF abundance following the various altitudes and after trapping by sorghum plant. The results showed that soil properties, AMF spore abundances and colonization fluctuated significantly at different altitudes. The most represented texture was sandy loam. The bimodal zone presented a homogeneous texture (sandy loam) in all its localities. Cacao soil chemical characteristics showed that, the highest nitrogen rate (0.47%;p 0.05, Scott-Knott test) was recorded at Melong in a monomodal zone while Tonga in the Western highlands displayed the lowest rate (0.13%). Soil P concentration was significantly high in monomodal zones (Mbanga and Melong). Soil pH level indicated that the soil from Tonga in the Western highlands was neutral (pH = 6.67), and soils of other localities under study were acidic with the lowest (4.75) pH level recorded at Melong in a monomodal zone. In soil samples, the highest spore density (1.03 spores/g soil) was observed at Ntui in Bimodal zone, while the lowest spore density (0.26 spores/g soil) was observed at Bafang in the Western highlands. Root colonization showed that the sample from Bokito in a bimodal zone displayed the best frequency of mycorrhization (86.11%) while the sample from Bafang in the Western highlands recorded the lowest (27.11%). The PCA analysis highlighted that available phosphorus, pH and altitude all strongly correlated with AMF root colonization ability and can be used as a predictor of AMF colonization ability in cacao rhizosphere.

Morphological Characterization of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere According to the Age of Xanthosoma sagittifolium L. Schott Plants in the Field

The objective of this work was to carry out a morphological characterization of arbuscular mycorrhizal fungi in the rhizosphere of Xanthosoma sagittifolium L. Schott plants. The plant material used was the white and red cultivars of X. sagittifolium, belonging to age intervals of 3 - 6, 6 - 9, and 9 - 12 months. Three harvest sites were chosen in the Central Region of Cameroon. In each site, soil from the rhizosphere and plant roots was collected in a randomized manner. In the field, the agronomic parameters were evaluated. The physicochemical characteristics of the soils, the mycorrhization index, and the morphological characterization of the mycorrhizal types of each site were carried out. The results obtained show that the agronomic growth parameters varied significantly using the Student Newman and Keuls Test depending on the harvest sites. The soils’ pH in all sites was acidic and ranged between 4.6 and 5.8. The Nkometou site has a loamy texture while the Olembe and Soa sites have loam-clay-sandy and loam-clay textures respectively. The highest mycorrhization frequencies appeared at the Nkometou site, with 75 and 87.33% of the white and red cultivars plant roots at 6 - 9 and 3 - 6 months. The relative abundance of AMF arbuscular mycorrhizal fungal spores in the rhizosphere of X. sagittifolium plants varied with age and cultivar. There were 673 spores between 9 - 12 months in Nkometou in the red cultivar. Six AMF genera were identified in all the different soils collected: Acaulospora sp., Funneliformis sp., Gigaspora sp., Glomus sp., Scutellospora sp., and Septoglomus sp. The genus Glomus sp. was the most present at all age intervals in both cultivars.

Diversity of Arbuscular Mycorrhizal Fungi Associated with Six Rice Cultivars in Italian Agricultural Ecosystem Managed with Alternate Wetting and Drying

Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was proposed to utilize water and nutrients more sustainable.In this study,we selected six rice cultivars(Centauro,Loto,Selenio,Vialone nano,JSendra and Puntal)grown under AWD conditions,and investigated their responsiveness to AM colonization and how they select diverse AM taxa.In order to investigate root-associated AM fungus communities,molecular cloning-Sanger sequencing on small subunit rDNA data were obtained from five out of the six rice cultivars and compared with Next Generation Sequencing(NGS)data,which were previously obtained in Vialone nano.The results showed that all the cultivars were responsive to AM colonization with the development of AM symbiotic structures,even if with differences in the colonization and arbuscule abundance in the root systems.We identified 16 virtual taxa(VT)in the soil compartment and 7 VT in the root apparatus.We emphasized that the NGS analysis gives additional value to the results thanks to a more in-depth reading of the less represented AM fungus taxa.

Moso bamboo expansion decreased soil heterotrophic respiration but increased arbuscular mycorrhizal mycelial respiration in a subtropical broadleaved forest

Moso bamboo(Phyllostachys Pubescens)expansion into adjacent forests has been widely reported to affect plant diversity and its association with mycorrhizal fungi in subtropical China,which will likely have significant impacts on soil respiration.However,there is still limited information on how Moso bamboo expansion changes soil respiration components and their linkage with microbial community composition and activity.Based on a mesh exclusion method,soil respirations derived from roots,arbuscular mycorrhizal(AM)mycelium,and free-living microbes were investigated in a pure Moso bamboo forest(expanded),an adjacent broadleaved forest(nonexpanded),and a mixed bamboo-broadleaved forest(expanding).Our results showed that bamboo expansion decreased the cumulative CO_(2)effluxes from total soil respiration,root respiration and soil heterotrophic respiration(by 19.01%,30.34%,and 29.92%on average),whereas increased those from AM mycelium(by 78.67%in comparison with the broadleaved forests).Bamboo expansion significantly decreased soil organic carbon(C)content,bacterial and fungal abundances,and enzyme activities involved in C,N and P cycling whereas enhanced the interactive relationships among bacterial communities.In contrast,the ingrowth of AM mycelium increased the activities ofβ-glucosidase and N-acetyl-β-glucosaminidase and decreased the interactive relationships among bacterial communities.Changes in soil heterotrophic respiration and AM mycelium respiration had positive correlations with soil enzyme activities and fungal abundances.In summary,our findings suggest that bamboo expansion decreased soil heterotrophic respiration by decreasing soil microbial activity but increased the contribution of AM mycelial respiration to soil C efflux,which may potentially increase soil C loss from AM mycelial pathway.

Effect of arbuscular mycorrhiza on the growth of Camptotheca acuminata seedlings

Camptotheca acuminata seeds were sown in sterilized sands in the greenhouse in February of 2005. After 90-day growth, seedlings were inoculated with three species of arbuscular mycorrhizal fungi （AMF）, Acaulospora mellea, Glomus diaphanum and Sclerocystis sinuosa.. The height, biomass, and absorptions of nitrogen and phosphorus of C. acuminata seedlings inoculated with AMF were investigated. The results showed that the formation of AM promoted the height growth and biomass accumulation of seedlings significantly and improved the absorption of phosphorus in seedlings. The height and biomass of mycorrhizal seedlings were 1.2 and 1.6 times higher than those of the non-mycorrhizal seedlings. The absorption of nitrogen was less influenced by the formation of AM. The nitrogen content in mycorrhizal seedling was equal to that of non-mycorrhizal seedlings. Compared with non-mycorrhizal seedlings, the nitrogen content of mycorrhizal seedlings inoculated with A. mellea changed considerably in the root, stem and leaves. The difference in nitrogen content was not significant between mycorrhizal seedlings inoculated with G. diaphanum and S. sinuosa. The AM formation stimulated the absorption of phosphorus, especially in roots, and also changed the allocation of nitrogen and phosphorus in different organs of seedlings. Compared with non-mycorrhizal seedlings, the ratio of nitrogen and phosphorus in mycorrhizal roots increased, but reduced in stem and leaves.

Effects of Arbuscular Mycorrhizal Fungi on the Physiology and Saponin Synthesis of Paris polyphylla var. yunnanensis at Different Nitrogen Levels

Arbuscular mycorrhizal fungi(AMF)are important members of the plant microbiome and affect the uptake and transfer of mineral elements by forming a symbiotic relationship with plant roots.Nitrogen(N),as an important min­eral element,can directly affect plant growth and development at different N levels.It has been confirmed that inoc­ulation with AMF can improve the efficiency of N utilization by plants.However,there are still fewer reports on the dynamic relationship between arbuscular mycorrhizal and plant secondary metabolites at different nitrogen levels.In this experiment,the physiological indexes and genes related to saponin synthesis were determined by applying differ­ent concentration gradients of nitrogen to the medicinal plant P.polyphylla var.yunnanensis infested by AMF as the test material.It was found that nitrogen addition increased the biomass,chlorophyll content,and nutrient content of above-and below-ground plant parts and increased the content of saponin content of P.polyphylla var.yunnanensis to some extent,but AMF inoculation increased the saponin content of P.polyphylla var.yunnanensis more significantly.AMF inoculation also promoted the expression of genes related to the saponin synthesis pathway,including 3-hy­droxy-3-methylglutaryl coenzyme A synthase(HMGS),squalene epoxidase 1(SE1),and cycloartenol synthase(CAS),which is in according with the accumulation of saponin in plants.It also may increase the saponin content of AMF plants by altering the expression of P450s and UGTs related to saponin synthesis.

Effect of Arbuscular Mycorrhiza on the Content of Nitrogen and Nitrogenous Matter in Amur Corktree Seedlings

[Objective] This study aimed to explore the effect of arbuscular mycorrhiza on the content of nitrogen and nitrogenous matter in amur corktree（Phellodendron amurense Rupr.）seedlings. [Method] The annual seedlings of Phellodendron amurense Rupr. were inoculated with four arbuscular mycorrhiza fungi in a pot experiment to study the influences of arbuscular mycorrhiza on the content of nitrogen and nitrogenous matter in Phellodendron amurense Rupr. [Result] After inoculation with arbuscular mycorrhiza fungi, the Phellodendron amurense Rupr. seedlings developed arbuscular mycorrhiza, leading to an enhancement of photosynthetic capacity. The leaf nitrogen content of those inoculated with Glomus mosseae increased to 1.28- 1.60 times as compared with the control. The chlorophyll content and chlorophyll a/b ratio were also raised, with an increase over 25% of chlorophyll a content. In addition, IAA content in plants increased to 1.65-2.41 times; and nitrate reductase activity was also enhanced, as well as soluble protein content, 1.67-2.49 times as high as the control, which improved the nitrogen metabolic ability, and promoted the plant growth, as well as the secondary metabolic ability. [Conclusion] This study provides a theoretical basis for the application of arbuscular mycorrhiza on Phellodendron amurense Rupr.

Effect of Different Arbuscular Mycorrhizal Fungi Strains on Mineral Nutrition and Antioxidant Enzymes of Chrysanthemum morifolium

[Objective] The purpose was to study the effects of different arbuscular mycorrhizal fungi strains（AMF）on the mineral nutrition and antioxidant enzymes of Chrysanthemum morifolium.[Method] The pot experiment was conducted in the greenhouse,C.morifolium ＇Jinba＇ was inoculated with five kinds of AMF,N,P,K,malondial dehyde（MDA）content,as well as the superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT） activity in roots,leaves and petals of C.morifolium were measured at seedling and flowering stages.[Result] The G.i,G.e and G.m treatments could promote mineral nutrient absorption,increase N,P,K content in roots,leaves and petals of C.morifolium compared with the control without inoculation.The G.d、G.e and G.m treatments could significantly reduce MDA content in roots and petals,thus alleviating membrane permeability and lipid peroxidation.The G.i treatments could also improve the SOD,POD and CAT activities of C.morifolium,thereby increasing the capability of scavenging oxygen free radicals.[Conclusion] According to the comprehensive analysis,G.i was screened out as the best strain to improve mineral nutrition and antioxidant enzyme activities of C.morifolium.

Research Progress on the Process and Mechanism of Arbuscular Mycorrhizal Fungi Colonizing Roots

The arbuscular mycorrhiza （AM） is a kind of fungi-plant associated sym- biont formed by the arbuscular mycorrhizal fungi and plants in soil. Present study was limited to the population and community level, mainly in horticulture, land recla- mation, forest and environmental restoration. Research progress was also made at the cellular level and molecular level. Process and related mechanism of mycorrhizal fungi infecting root were reviewed, and future study on the mechanism of arbuscular mycorrhizal fungi infecting root should be continued.

Effects of Arbuscular Mycorrhizal Fungi(AMF) on Growth of Upland Rice under Soil Pb Contamination

[Objective] This study aimed to investigate the effects of arbuscular mycor-rhizal fungi （AMF） on growth of upland rice under soil Pb contamination. [Method] Using potting method, the effects of Glomus mosseae on the growth of Oryzal sati-va L. under different soil Pb concentrations （0, 300, 600 mg/kg） were investigated. [Result] According to the results, the mycorrhizal colonization rate of upland rice in-oculated with Glomus mosseae was significantly reduced （P〈0.05） with the increase of Pb concentration in soil. Compared with non-inoculation treatment, inoculation of arbuscular mycorrhizal fungi significantly improved the biomass of upland rice and Pb concentration of upland rice roots with addition of 300 mg/kg Pb but significantly reduced Pb concentration of upland rice shoots, which was consistent with the re-duced R/S （P〈0.05）; with addition of 600 mg/kg Pb, inoculation of arbuscular mycor-rhizal fungi significantly improved the biomass of upland rice roots （P〈0.05） but sig-nificantly reduced Pb concentration of upland rice shoots and roots （P〈0.05）; there was no significant difference in R/S between inoculation treatment and non-inocula-tion treatment. [Conclusion] This study indicated that inoculating Glomus mosseae under certain Pb concentrations could to some extent al eviate the toxic effects of Pb on Oryzal sativa L.

Effects of Arbuscular Mycorrhizal Fungi on Upland Rice Oxidative Stress Induced by Cu and Pb Contamination in Soil

[Objective] This study aimed to investigate the effect of arbuscular mycor-rhizal fungi on upland rice oxidative stress induced by Cu and Pb contamination in soil. [Method] The upland rice seeds were sowed in pots, in which the soil was previously mixed with a certain amount of Glomus mosseae and 0, 100 and 200 mg/kg Cu, or 0, 300 and 600 mg/kg Pb. In the control treatment, Glomus mosseae was inactivated before mixed into the soil. Then, the physiological and chemical properties of the aboveground parts of rice plants were measured at mature stage. [Result] Compared with the control treatment （NM）, Glomus mosseae （GM） treat-ment inhibited the POD, CAT and SOD activity while increased the soluble protein content under 100 mg/kg Cu and 300 mg/kg Pb treatment, improved the POD and CAT activity and soluble protein content while decreased SOD activity under 200 mg/kg Cu. SOD and POD activity showed no significant difference between NM and GM treatment under 600 mg/kg Pb, but the CAT activity was enhanced and soluble protein content was decreased. [Conclusion] This study wil provide theoretical refer-ence for bioremediation of soil heavy metal pol ution.

EFFECT OF HOST PLANTS AND INOCULUM FORMS ON THE INOCULUM POTENTIAL OF ARBUSCULAR MYCORRHIZAL FUNGI

The effects of inoculum forms (single-spore, multi-spores, or colonized root pieces) and host plants (Nicotiana tabacum L., Sorghum sudanense (Piper) Stapf, and Trifolium repens L.) on the development and inoculum potential (IP) of the arbuscular mycorrhizal fungi (AMF) :Glo-mus macrocarpum Tul & Tul, donuis mosseae (Nicol & Gerd.) Gerdemann & Trappe, Glomus ver-siforme (Karsten) Berch, and Sclerocystis sinu/osa Gerdemann & Bakhi cultured in pots were investigated. The lag phase of treatment with 50 spores or 0.5 g (fresh weight) of colonized root pieces was 4 weeks, much shorter than that of the treatment with 1 spore (8 weeks); the value of IP (VIP) and percentage of root colonization (PRC) of the former were greater than those of the latter. Only on the early stages of colonization was there difference between the 50 spores and the 0.5 g (fresh weight) of colonized root piece inoculation treatments. The EP per plant inoculated with 0.5 g (fresh weight) of colonized root pieces of AMF was greater than that of the other two treatments except G. versiforme on Nicotiana tabacum, while the PRC of the plants inoculated with 50 spores and 0.5 g (fresh weight) of colonized root pieces of AMF was higher than that of the 1 spore inoculation after 10 weeks. Trie VIP of AMF on Trifolium repens was significantly higher than that on the other two hosts. The VIP of G. mosseae, G. versiforme, and S. sinuosa was respectively greater than that of G. macrocarpum. This suggested that different species of AMF produced different VIP of the inoculum . Nicotiana tabacum was much better than the other host plants which used to be inoculated with single spore, and to produce inocula of AMF.

Review of Research Methods on Biodiversity of Arbuscular Mycorrhizal Fungi

Arbuscular mycorrhizal fungi （AMF） are essential functional microbiology in natural ecosystems. It is very important to research community composition and di- versity of AMF for achieving sustainable development of ecosystems. The paper described several methods of researching the diversity of AMF, especially for molecular techniques, reviewed the application status of these methods in AMF research, and pointed out that the effective combination of morphological and molecular methods could better reveal the biodiversity and ecological functions of AMF in natural ecosystems.

Effects of Arbuscular Mycorrhizal Fungi (AMF) on Distribution of Pb Forms in Rhizosphere of Upland Rice

[Objective] The aim was to study the effects of AMF on distribution of Pb in different chemical forms in rhizosphere soil of upland rice. [Method] A pot experiment was conducted to explore effects of AMF inoculation on distribution of Pb in different forms in rhizosphere of rice (Oryzal sativa L.) with Pb in different concentrations (0, 300 and 600 mg/kg). [Result] With inoculation adopted, mycorrzhial colonization rate of upland rice under Pb pdlution root declined substantially with Pb increasing in soils (P<0.05). Compared with non-inoculation, rhizosphere pH significantly enhanced by inoculation; when Pb was at 300 mg/kg, glomalin content in soils improved significantly by inoculation; when Pb was at 600 mg/kg, glomalin content in soils declined substantially (P<0.05). In addition, inoculation significantly improved contents of Pb in exchangeable and organic forms, but lowered Pb in carbonate bound and Fe-Mn oxides bound forms (P<0.05). [Conclusion] The research indicated that AMF inoculation would change distribution of Pb in different forms in rhizosphere soils of upland rice.

Effects of Seedling-cake Integrated with Arbuscular Mycorrhizal Fungi on Resistance of Bacterial Wilt in Pepper (Capsicum annuum L.)

[Objective] The aim was to investigated the effects of seedling-cake with AMF on resistance of pepper （Capsicum annuum L.） to bacterial wilt. [Methods] A pot experiment was conducted to evaluate the dynamics of mycorrhizal colonization, disease index, morbidity rate, control effect and several agronomic traits of mycorrhizal seedlings after inoculation with RS in two kinds of seeding-cakes with AMF. [Results] Two kinds of seedling-cakes formed steadily mycorrhizal colonization after inoculation, which enhanced disease-resistance and decreased morbidity rate and disease index in different degrees. What’s more, the performance of self-made seedling-cakes was obviously better than that of commercial seedling-cakes. [Conclusions] Self-made seedling-cakes with AMF have superior performance on bacterial wilt resistance of pepper, which should be made use of in other crops extensively.

Arsenic uptake by arbuscular mycorrhizal maize (Zea mays L.) grown in an arsenic-contaminated soil with added phosphorus

The effects of arbuscular mycorrhizal （AM） fungus （Glomus mosseae） and phosphorus （P） addition （100 mg/kg soil） on arsenic （As） uptake by maize plants （Zea mays L.） from an As-contaminated soil were examined in a glasshouse experiment. Non-mycorrhizal and zero-P addition controls were included. Plant biomass and concentrations and uptake of As, P, and other nutrients, AM colonization, root lengths, and hyphal length densities were determined. The results indicated that addition of P significantly inhibited root colonization and development of extraradical mycelium. Root length and dry weight both increased markedly with mycorrhizal colonization under the zero-P treatments, but shoot and root biomass of AM plants was depressed by P application. AM fungal inoculation decreased shoot As concentrations when no P was added, and shoot and root As concentrations of AM plants increased 2.6 and 1.4 times with P addition, respectively. Shoot and root uptake of P, Mn, Cu, and Zn increased, but shoot Fe uptake decreased by 44.6%, with inoculation, when P was added. P addition reduced shoot P, Fe, Mn, Cu, and Zn uptake of AM plants, but increased root Fe and Mn uptake of the nonmycorrhizal ones. AM colonization therefore appeared to enhance plant tolerance to As in low P soil, and have some potential for the phytostabilization of As-contaminated soil, however, P application may introduce additional environmental risk by increasing soil As mobility.

The role of arbuscular mycorrhiza on change of heavy metal speciation in rhizosphere of maize in wastewater irrigated agriculture soil

To understand the roles of mycorrhiza in metal speciation in the rhizosphere and the impact on increasing host plant tolerance against excessive heavy metals in soil, maize(Zea mays L.) inoculated with arbuscular mycorrhizal fungus(Glomus mosseae) was cultivated in heavy metal contaminated soil. Speciations of copper, zinc and lead in the soil were analyzed with the technique of sequential extraction. The results showed that,in comparison to the bolked soil, the exchangeable copper increased from 26% to 43% in non-infected and AM-infected rhizoshpere respectively; while other speciation (organic, carbonate and Fe-Mn oxide copper) remained constant and the organic bound zinc and lead also increased but the exchangeable zinc and lead were undetectable. The organic bound copper, zinc and lead were higher by 15%, 40% and 20%, respectively, in the rhizosphere of arbuscular mycorrhiza infected maize in comparison to the non-infected maize. The results might indicate that mycorrhiza could protect its host plants from the phytotoxicity of excessive copper, zinc and lead by changing the speciation from bio-available to the non-bio-available form. The fact that copper and zinc accumulation in the roots and shoots of mycorrhia infected plants were significantly lower than those in the non-infected plants might also suggest that mycorrhiza efficiently restricted excessive copper and zinc absorptions into the host plants. Compared to the non-infected seedlings, the lead content of infected seedlings was 60% higher in shoots. This might illustrate that mycorrhiza have a different mechanism for protecting its host from excessive lead phytotoxicity by chelating lead in the shoots.

Effect of Arbuscular Mycorrhizal Inoculation on Plant Growth and Phthalic Ester Degradation in Two Contaminated Soils

A 60-day pot experiment was carried out using di-(2-ethylhexyl) phthalate (DEHP) as a typical organic pollutant phthalic ester and cowpea (Vigna sinensis) as the host plant to determine the effect of arbuscular mycorrhizal inoculation on plant growth and degradation of DEHP in two contaminated soils, a yellow-brown soil and a red soil. The air-dried soils were uniformly sprayed with different concentrations of DEHP, inoculated or left uninoculated with an arbuscular mycorrhizal (AM) fungus, and planted with…