Direct measurement of the three-dimensional distribution of leaf area density and light conditions in a mature oak stand by the cube method

Although the distributions of foliage and light play major roles in various forest functions,accurate,nondestructive measurement of these distributions is difficult due to the complexity of the canopy structure.To evaluate the foliage and light distributions directly and nondestructively in a mature oak stand,we used the cube method by dividing the forest canopy into small cubes(50 cm per side)and directly measured leaf area density(LAD,the total one-sided leaf area per unit volume,i.e.,cube)and relative irradiance(RI)within each cube.The distribution of LAD and of RI was highly heterogeneous,even at the same canopy height.This heterogeneity reflected the presence of foliage clusters associated with multiple forking branches.The relationship between cumulative LAD at the canopy surface and average RI followed the Beer-Lambert law.The mean light extinction coefficient(K)was 0.32.However,K was overestimated by more than double(0.80)when calculated based on the classical method using RI at the forest floor.This overestimation was caused by the lower RI due to light absorption by nonleaf plant parts below the canopy.Our findings on the complex foliage and light distributions in canopy layers should help improve the accuracy of RI and K measurements and thus more accurate predictions of environmental responses and forest functions.

Effect of layer thickness and voxel size inversion on leaf area density based on the voxel-based canopy profiling method

Voxel-based canopy profiling is commonly used to determine small-scale leaf area.Layer thickness and voxel size impact accuracy when using this method.Here,we determined the optimal combination of layer thickness and voxel size to estimate leaf area density accurately.Terrestrial LiDAR Stonex X300 was used to generate point cloud data for Masson pines(Pinus massoniana).The canopy layer was stratified into 0.10-1.00-m-thick layers,while voxel size was 0.01-0.10 m.The leaf area density of individual trees was estimated using leaf area indices for the upper,middle,and lower canopy and the overall canopy.The true leaf area index,obtained by layered harvesting,was used to verify the inversion results.Leaf area density was inverted by nine combinations of layer thickness and voxel size.The average relative accuracy and mean estimated accuracy of these combined inversion results exceeded 80%.When layer thickness was 1.00 m and voxel size 0.05 m,inversion was closest to the true value.The average relative accuracy was 92.58%,mean estimated accuracy 98.00%,and root mean square error 0.17.The combination of leaf area density and index was accurately retrieved.In conclusion,nondestructive voxel-based canopy profiling proved suitable for inverting the leaf area density of Masson pine in Hetian Town,Fujian Province.

Visual rating and the use of image analysis for assessing canopy density in a pecan provenance collection during leaf fall

A collection representing the native range of pecan was planted at the US DA-ARS Southeastern Fruit and Tree Nut Research Station,Byron,GA.The collection(867 trees)is a valuable genetic resource for characterizing important horticultural traits.Canopy density during leaf fall is important as the seasonal canopy dynamics provides insights to environmental cues and breeding potential of germplasm.The ability of visual raters to estimate canopy density on a subset of the provenance collection(76 trees)as an indicator of leaf shed during autumn along with image analysis values was explored.Mean canopy density using the image analysis software was less compared to visual estimates(11.9%vs 18.4%,respectively).At higher canopy densities,the raters overestimated foliage density,but overall agreement between raters and measured values was good(ρc=0.849 to 0.915),and inter-rater reliability was high(R^(2)=0.910 to 0.953).The provenance from Missouri(MOL),the northernmost provenance,had the lowest canopy density in November,and results show that the higher the latitude of the provenance,the lower the canopy density.Based on regression,the source provenance latitude explained 0.609 of the variation using image analysis,and0.551 to 0.640 when based on the rater estimates of canopy density.Visual assessment of pecan canopy density due to late season leaf fall for comparing pecan genotypes provides accurate and reliable estimates and could be used in future studies of the whole provenance collection.

Microvessel changes in the gerbil hippocampus after cerebral ischemia and reperfusion by Buyang Huanwu decoction pretreatment

Buyang Huanwu decoction （BYHWD） is a classic recipe for the prevention and treatment of ischemic cerebrovascular disease. Gerbils were pretreated with BYHWD, and then subjected to cerebral ischemia and reperfusion. Microvascular changes were determined with laser Doppler monitoring, tannic acid-ferric chloride mordant, and electron microscopy. Results showed that BYHWD pretreatment could enhance the function of hippocampal microvessels, prevent injury, and increase microvasular density and microvasular area density. Thus, these results suggest that BYHWD pretreatment could prevent microvascular occlusion, enhance the capacity of microvascular reperfusion, increase cerebral blood flow, and inhibit neuronal damage, and may be an effective therapy against brain ischemic injury.

Timing of N Application Affects Net Primary Production of Soybean with Different Planting Densities

Understanding the relationship between the timing of N fertilizer applications and crop primary production is crucial for achieving high yield and N use efifciency in agriculture. This study investigated the effects of starting-N plus topdressing N applications （as compared to the common practice of all basal application） on soybean photosynthetic capacity under different planting densities. A ifeld experiment was conducted in two growing seasons （2011 and 2012）, and the soybean （Glycine max L. Merrill） cultivar was Dongnong 52, three planting densities （20, 25 and 30 plants m-2）, and four N fertilizer application patterns （all N fertilizer of 6 g N m-2 as basal fertilizer, all N fertilizer as topdressing at beginning pod stage （R3）, 1.8 g N m-2 as basal fertilizer and 4.2 g N m-2 as topdressing at stage R3 and full pod stage （R4）, respectively）. The results indicated that under the same planting density, compared to applying all N as basal fertilizer, the application of starter-N plus topdressing N substantially reduced the rate of pod abscission, and enhanced leaf area index （LAI） signiifcantly at beginning seed stage （R5） （P〈0.05）, net assimilation rate （NAR） during stages R4-full seed stage （R6） （P〈0.05）, contribution rate of post-seed iflling assimilate to seed （CPA） （P〈0.05）, and yield （P〈0.05）. Applying topdressing N at stage R4 resulted in higher net primary production and yield than applying topdressing N at stage R3. When applying starter-N plus topdressing N at planting density of 25 plants m-2, LAI after stage R5 and NAR after stage R4 were increased by 5.92-16.3%（P〈0.05） and 13.7-26.6%（P〈0.05） with the planting density of 20 plants m-2, respectively, and yield was 8.46-14.0%（P〈0.05） higher than that under 20 plants m-2. When planting density increased to 30 plants m-2, only LAI during stages R4-R5 and NAR during stages R4-R5 increased by applying starter-N plus topdressing N, while the other indexes declined. Overall, results of this study demonstrated that applying starter-N plus topdressing N could signiifcantly enhance soybean photosynthetic capacity after stage R5 at planting density of 25 plants m-2.

Discussion on Common Problems in Risk Assessment of Lightning Disaster

The problems in calculating parameters of equivalent collection area,earth resistivity and lightning protection category as well as their effects on lightning disaster risk assessment were analyzed,and practical examples proved the effects of those differences on lightning protection identification,intercept efficiency calculation in evaluating lightning disaster risk. In the meantime,several new concepts,such as the height of buildings for lightning protection were defined,and a fixed radius value was set to the ground flash density for calculation,establishing the ground flash density formula to solve the problems in parameter calculation,which would be beneficial to promote the standardization of lightning disaster risk assessment.