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Ionic Liquid-Enhanced Assembly of Nanomaterials for Highly Stable Flexible Transparent Electrodes 被引量:1
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作者 Jianmin Yang Li Chang +2 位作者 Xiqi Zhang Ziquan Cao Lei Jiang 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第7期441-455,共15页
The controlled assembly of nanomaterials has demon-strated significant potential in advancing technological devices.However,achieving highly efficient and low-loss assembly technique for nanomate-rials,enabling the cr... The controlled assembly of nanomaterials has demon-strated significant potential in advancing technological devices.However,achieving highly efficient and low-loss assembly technique for nanomate-rials,enabling the creation of hierarchical structures with distinctive func-tionalities,remains a formidable challenge.Here,we present a method for nanomaterial assembly enhanced by ionic liquids,which enables the fabrication of highly stable,flexible,and transparent electrodes featuring an organized layered structure.The utilization of hydrophobic and non-volatile ionic liquids facilitates the production of stable interfaces with water,effectively preventing the sedimentation of 1D/2D nanomaterials assembled at the interface.Furthermore,the interfacially assembled nanomaterial monolayer exhibits an alternate self-climbing behavior,enabling layer-by-layer transfer and the formation of a well-ordered MXene-wrapped silver nanowire network film.The resulting composite film not only demonstrates exceptional photoelectric performance with a sheet resistance of 9.4Ωsq^(-1) and 93%transmittance,but also showcases remarkable environmental stability and mechanical flexibility.Particularly noteworthy is its application in transparent electromagnetic interference shielding materials and triboelectric nanogenerator devices.This research introduces an innovative approach to manufacture and tailor functional devices based on ordered nanomaterials. 展开更多
关键词 Ionic liquids assembly Silver nanowires MXene nanosheets Flexible transparent electrodes
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Response and assembly of abundant and rare taxa in Zaopei under different combination patterns of Daqu and pit mud:from microbial ecology to Baijiu brewing microecosystem 被引量:1
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作者 Yu Mu Jun Huang +5 位作者 Rongqing Zhou Suyi Zhang Hui Qin Hanlan Tang Qianglin Pan Huifang Tang 《Food Science and Human Wellness》 SCIE CSCD 2024年第3期1439-1452,共14页
The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Z... The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare taxa.Here,an experiment on industrial size was designed to assess the effects of 6 combinations(3 kinds of Daqu×2 kinds of PM)on the composition and assembly of different taxa,as well as the flavor profile.The results showed that Zaopei's microbiota was composed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subcommunities were significantly affected by combination patterns.The assembly processes of abundant/rare taxa and bacterial/fungal communities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria assembly.Furthermore,specific combination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare bacteria.These findings highlighted that rare bacteria might be the keystone in involving community interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial ecology. 展开更多
关键词 Bioaugmented Daqu Artificial pit mud Abundant and rare taxa Community assembly Metabolic function
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Design and assembly of a nested imaging X-ray telescope for the Hot Universe Baryon Surveyor mission 被引量:1
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作者 Jun Yu Ruohui Xian +8 位作者 Xiaoqiang Wang Yifan Wang Zhanshan Wang Wei Zhang Yibo Cai Jing Yang Xi Lu Wei Wang Wei Cui 《Astronomical Techniques and Instruments》 CSCD 2024年第3期157-165,共9页
The Hot Universe Baryon Surveyor (HUBS) mission will carry a nested X-ray telescope capable of observing an energy range from 0.5 keV to 2 keV to study hot baryon evolution. In this paper, we report the latest progres... The Hot Universe Baryon Surveyor (HUBS) mission will carry a nested X-ray telescope capable of observing an energy range from 0.5 keV to 2 keV to study hot baryon evolution. In this paper, we report the latest progress in the design and construction of nested X-ray telescopes which were designed to use a three-stage conic-approximation type assembly to simplify the manufacturing process. The mirror substrate is made using the thermal glass slumping method, with mirrors characterized by a root-mean-square roughness of 0.3 nm, with expected high reflectivity and good thermal stability. We also discuss methods of telescope construction and conduct a deformation analysis of the manufactured mirror. The in situ measurement system program is developed to guide the telescope assembly process. 展开更多
关键词 Nested X-ray telescope Thermal glass slumping Telescope assembly In situ measurement
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Self-assembly of tau fragments as a key pathologic event in tauopathies
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作者 Ly Thi Huong Luu Le Jung Hoon Lee Min Jae Lee 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第12期2565-2566,共2页
Tau protein is encoded by the microtubuleassociated protein tau(MAPT)gene and stabilizes microtubules in the neurons.Tau has unique biophysical properties that make it both highly water-soluble and positively charged.... Tau protein is encoded by the microtubuleassociated protein tau(MAPT)gene and stabilizes microtubules in the neurons.Tau has unique biophysical properties that make it both highly water-soluble and positively charged.Although tau is an intrinsically disordered protein lacking defined secondary structures,it can acquire highly organized-sheet structures and stack into a filamentous inclusion such as a paired helical filament(PHF). 展开更多
关键词 TAU PATHOLOGIC assembly
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A telomere-to-telomere genome assembly of Zhonghuang 13,a widely-grown soybean variety from the original center of Glycine max
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作者 Anqi Zhang Tangchao Kong +21 位作者 Baiquan Sun Shizheng Qiu Jiahe Guo Shuyong Ruan Yu Guo Jirui Guo Zhishuai Zhang Yue Liu Zheng Hu Tao Jiang Yadong Liu Shuqi Cao Shi Sun Tingting Wu Huilong Hong Bingjun Jiang Maoxiang Yang Xiangyu Yao Yang Hu Bo Liu Tianfu Han Yadong Wang 《The Crop Journal》 SCIE CSCD 2024年第1期142-153,共12页
Soybean(Glycine max)stands as a globally significant agricultural crop,and the comprehensive assembly of its genome is of paramount importance for unraveling its biological characteristics and evolutionary history.Nev... Soybean(Glycine max)stands as a globally significant agricultural crop,and the comprehensive assembly of its genome is of paramount importance for unraveling its biological characteristics and evolutionary history.Nevertheless,previous soybean genome assemblies have harbored gaps and incompleteness,which have constrained in-depth investigations into soybean.Here,we present Telomere-to-Telomere(T2T)assembly of the Chinese soybean cultivar Zhonghuang 13(ZH13)genome,termed ZH13-T2T,utilizing PacBio Hifi and ONT ultralong reads.We employed a multi-assembler approach,integrating Hifiasm,NextDenovo,and Canu,to minimize biases and enhance assembly accuracy.The assembly spans 1,015,024,879 bp,effectively resolving all 393 gaps that previously plagued the reference genome.Our annotation efforts identified 50,564 high-confidence protein-coding genes,707 of which are novel.ZH13-T2T revealed longer chromosomes,421 not-aligned regions(NARs),112 structure variations(SVs),and a substantial expansion of repetitive element compared to earlier assemblies.Specifically,we identified 25.67 Mb of tandem repeats,an enrichment of 5S and 48S rDNAs,and characterized their genotypic diversity.In summary,we deliver the first complete Chinese soybean cultivar T2T genome.The comprehensive annotation,along with precise centromere and telomere characterization,as well as insights into structural variations,further enhance our understanding of soybean genetics and evolution. 展开更多
关键词 SOYBEAN Telomere-to-Telomere assembly Zhonghuang 13 Structure variations
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An Improved Chromosome-Level Genome Assembly and Annotation of Belted Beard Grunt(Hapalogenys analis)
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作者 GAO Tianxiang WANG Yiting +3 位作者 SHI Huilai PING Hongling LIU Qi ZHANG Yang 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期1026-1034,共9页
Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and P... Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and PacBio sequencing technique to assemble a high-quality,chromosome-level genome for this species.The 539 Mb genome had a contig N50 with a size of 3.43 Mb,while 755 contigs clustered into 24 chromosomal groups with an anchoring rate of 99.02%.Of the total genomic sequence,132.74Mb(24.39%)were annotated as repeat elements.A total of 21360 protein-coding genes were identified,of which 20787 genes(97.32%)were successfully annotated to public databases.The BUSCO evaluation indicated that 96.90%of the total orthologous genes were matched.The phylogenetic tree representing H.analis and 14 other bony fish species indicated that the H.analis genome contained 364 expanded gene families related to olfactory receptor activity,compared with the common ancestor of H.analis and Sciaenidae.Comparative genomic analysis further identified 3584 contracted gene families.Branch-site modeling identified 277 genes experiencing positive selection,which may facilitate the adaptation to rocky reef environments.The genome reported here is helpful for ecological and evolutionary studies of H.analis. 展开更多
关键词 Hapalogenys analis de novo assembly PacBio comparative genomics
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Optimization of Fixture Number in Large Thin-Walled Parts Assembly Based on IPSO
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作者 Changhui Liu Jing Wang +3 位作者 Ying Zheng Ke Jin Jianbo Yu Jianfeng Liu 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第2期213-227,共15页
There are lots of researches on fixture layout optimization for large thin-walled parts.Current researches focus on the positioning problem,i.e.,optimizing the positions of a constant number of fixtures.However,how to... There are lots of researches on fixture layout optimization for large thin-walled parts.Current researches focus on the positioning problem,i.e.,optimizing the positions of a constant number of fixtures.However,how to determine the number of fixtures is ignored.In most cases,the number of fixtures located on large thin-walled parts is determined based on engineering experience,which leads to huge fixture number and extra waste.Therefore,this paper constructs an optimization model to minimize the number of fixtures.The constraints are set in the optimization model to ensure that the part deformation is within the surface profile tolerance.In addition,the assembly gap between two parts is also controlled.To conduct the optimization,this paper develops an improved particle swarm optimization(IPSO)algorithm by integrating the shrinkage factor and adaptive inertia weight.In the algorithm,particles are encoded according to the fixture position.Each dimension of the particle is assigned to a sub-region by constraining the optional position range of each fixture to improve the optimization efficiency.Finally,a case study on ship curved panel assembly is provided to prove that our method can optimize the number of fixtures while meeting the assembly quality requirements.This research proposes a method to optimize the number of fixtures,which can reduce the number of fixtures and achieve deformation control at the same time. 展开更多
关键词 assembly quality Large thin-walled parts Fixture layout PSO FEM
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Gapless Genome Assembly of ZH8015 and Preliminary Multi-Omics Analysis to Investigate ZH8015's Responses Against Brown Planthopper Infestation
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作者 LI Dian DUAN Wenjing +5 位作者 LIU Qun’en WU Weixun ZHAN Xiaodeng SUN Lianping ZHANG Yingxin CHENG Shihua 《Rice science》 SCIE CSCD 2024年第3期317-327,I0042-I0045,共15页
Accurate genomic information is essential for advancing genetic breeding research in specific rice varieties.This study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bi... Accurate genomic information is essential for advancing genetic breeding research in specific rice varieties.This study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bio HiFi,Hi-C,and ONT(Oxford Nanopore Technologies)ultra-long sequencing technologies,annotating 43037 gene structures.Subsequently,utilizing this genome along with transcriptomic and metabolomic techniques,we explored ZH8015's response to brown planthopper(BPH)infestation.Continuous transcriptomic sampling indicated significant changes in gene expression levels around 48 h after BPH feeding.Enrichment analysis revealed particularly significant alterations in genes related to reactive oxygen species scavenging and cell wall formation.Metabolomic results demonstrated marked increases in levels of several monosaccharides,which are components of the cell wall and dramatic changes in flavonoid contents.Omics association analysis identified differentially expressed genes associated with key metabolites,shedding light on ZH8015's response to BPH infestation.In summary,this study constructed a reliable genome sequence resource for ZH8015,and the preliminary multi-omics results will guide future insect-resistant breeding research. 展开更多
关键词 brown planthopper gapless genome genome assembly multi-omics Nilaparvata lugens rice
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Engineering vascularized organotypic tissues via module assembly
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作者 Zhenzhen Zhou Changru Liu +2 位作者 Yuting Guo Yuan Pang Wei Sun 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2024年第1期155-175,共21页
Adequate vascularization is a critical determinant for the successful construction and clinical implementation of complex organotypic tissue models. Currently, low cell and vessel density and insufficient vascular mat... Adequate vascularization is a critical determinant for the successful construction and clinical implementation of complex organotypic tissue models. Currently, low cell and vessel density and insufficient vascular maturation make vascularized organotypic tissue construction difficult,greatly limiting its use in tissue engineering and regenerative medicine. To address these limitations, recent studies have adopted pre-vascularized microtissue assembly for the rapid generation of functional tissue analogs with dense vascular networks and high cell density. In this article, we summarize the development of module assembly-based vascularized organotypic tissue construction and its application in tissue repair and regeneration, organ-scale tissue biomanufacturing, as well as advanced tissue modeling. 展开更多
关键词 vascularized organotypic tissue module assembly regenerative medicine tissue engineering
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Experimental Study on the Electrochemical Performance of PEMFC under Different Assembly Forces
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作者 Tongze Su Jiaran Liu +3 位作者 Yanqiang Wei Yihuizi Li Weichao Luo Jinzhu Tan 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第3期265-274,共10页
Proton exchange membrane fuel cell(PEMFC)is of paramount significance to the development of clean energy.The components of PEMFC are assembled using many pairs of nuts and bolts.The assembly champing bolt torque is cr... Proton exchange membrane fuel cell(PEMFC)is of paramount significance to the development of clean energy.The components of PEMFC are assembled using many pairs of nuts and bolts.The assembly champing bolt torque is critical to the electrochemical performance and mechanical stability of PEMFC.In this paper,a PEMFC with the threechannel serpentine flow field was used and studied.The different assembly clamping bolt torques were applied to the PEMFC in three uniform assembly bolt torque and six non-uniform assembly bolt torque conditions,respectively.And then,the electrochemical performance experiments were performed to study the effect of the assembly bolt torque on the electrochemical performance.The test results show that the assembly bolt torque significantly affected the electrochemical performance of the PEMFC.In uniform assembly bolt torque conditions,the maximal power density increased initially as the assembly bolt torque increased,and then decreased on further increasing the assembly torque.It existed the optimum assembly torque which was found to be 3.0 N·m in this work.In non-uniform assembly clamping bolt torque conditions,the optimum electrochemical performance appeared in the condition where the assembly torque of each bolt was closer to be 3.0 N·m.This could be due to the change of the contact resistance between the gas diffusion layer and bipolar plate and mass transport resistance for the hydrogen and oxygen towards the catalyst layers.This work could optimize the assembly force conditions and provide useful information for the practical PEMFC stack assembly. 展开更多
关键词 Proton exchange membrane fuel cell assembly force EXPERIMENT Electrochemical performance
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A telomere-to-telomere genome assembly of Hongyingzi,a sorghum cultivar used for Chinese Baijiu production
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作者 Yanqing Ding Yilin Wang +12 位作者 Jianxia Xu Feng Jiang Wenzhen Li Qiaoling Zhang Longping Yang Zhenyu Zhao Bin Cheng Ning Cao Xu Gao Xin Zhang Guihua Zou Fan Yang Liyi Zhang 《The Crop Journal》 SCIE CSCD 2024年第2期635-640,共6页
Sorghum(Sorghum bicolor(L.)Moench)is a world cereal crop used in China for producing Baijiu,a distilled spirit.We report a telomere-to-telomere genome assembly of the Baijiu cultivar Hongyingzi,HYZ-T2T,using ultralong... Sorghum(Sorghum bicolor(L.)Moench)is a world cereal crop used in China for producing Baijiu,a distilled spirit.We report a telomere-to-telomere genome assembly of the Baijiu cultivar Hongyingzi,HYZ-T2T,using ultralong reads.The 10 chromosome pairs contained 33,462 genes,of which 93%were functionally annotated.The 20 telomeres and 10 centromeric regions on the HYZ-T2T chromosomes were predicted and two consecutive large inversions on chromosome 2 were characterized.A 65-gene reconstruction of the metabolic pathway of tannins,the flavor substances in Baijiu,was performed and may advance the breeding of sorghum cultivars for Baijiu production. 展开更多
关键词 SORGHUM Telomere-to-telomere assembly Hongyingzi(HYZ) Chinese Baijiu-making cultivar
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Designing Membrane Electrode Assembly for Electrochemical CO_(2)Reduction:a Review
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作者 Xuerong Wang Shulin Zhao +4 位作者 Tao Guo Luyao Yang Qianqian Zhao Yuping Wu Yuhui Chen 《Transactions of Tianjin University》 EI CAS 2024年第2期117-129,共13页
Currently, the electrochemical CO_(2) reduction reaction (CO_(2) RR) can realize the resource conversion of CO_(2) , which is a promising approach to carbon resource use. Important advancements have been made in explo... Currently, the electrochemical CO_(2) reduction reaction (CO_(2) RR) can realize the resource conversion of CO_(2) , which is a promising approach to carbon resource use. Important advancements have been made in exploring the CO_(2) RR performance and mechanism because of the rational design of electrolyzer systems, such as H-cells, flow cells, and catalysts. Considering the future development direction of this technology and large-scale application needs, membrane electrode assembly (MEA) systems can improve energy use efficiency and achieve large-scale CO_(2) conversion, which is considered the most promising technology for industrial applications. This review will concentrate on the research progress and present situation of the MEA component structure. This paper begins with the composition and construction of a gas diff usion electrode. Then, the application of ion-exchange membranes in MEA is introduced. Furthermore, the eff ects of pH and the anion and cation of the anolyte on MEA performance are explored. Additionally, we present the anode reaction type in MEA. Finally, the challenges in this field are summarized, and upcoming trends are projected. This review should offer researchers a clearer picture of MEA systems and provide important, timely, and valuable insights into rational electrolyzer design to facilitate further development of CO_(2) electrochemical reduction. 展开更多
关键词 CO_(2)reduction ELECTROCATALYSIS Membrane electrode assembly
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Digital Twin Modeling and Simulation Optimization of Transmission Front and Middle Case Assembly Line
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作者 Xianfeng Cao Meihua Yao +2 位作者 Yahui Zhang Xiaofeng Hu Chuanxun Wu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第6期3233-3253,共21页
As the take-off of China’s macro economy,as well as the rapid development of infrastructure construction,real estate industry,and highway logistics transportation industry,the demand for heavy vehicles is increasing ... As the take-off of China’s macro economy,as well as the rapid development of infrastructure construction,real estate industry,and highway logistics transportation industry,the demand for heavy vehicles is increasing rapidly,the competition is becoming increasingly fierce,and the digital transformation of the production line is imminent.As one of themost important components of heavy vehicles,the transmission front andmiddle case assembly lines have a high degree of automation,which can be used as a pilot for the digital transformation of production.To ensure the visualization of digital twins(DT),consistent control logic,and real-time data interaction,this paper proposes an experimental digital twin modeling method for the transmission front and middle case assembly line.Firstly,theDT-based systemarchitecture is designed,and theDT model is created by constructing the visualization model,logic model,and data model of the assembly line.Then,a simulation experiment is carried out in a virtual space to analyze the existing problems in the current assembly line.Eventually,some improvement strategies are proposed and the effectiveness is verified by a new simulation experiment. 展开更多
关键词 Transmission front and middle case assembly line digital twin(DT) simulating optimization intelligent manufacturing
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Evaluating Methods of Visual Assistance for Workers to Improve Quality and Usability in Individualized Kitchen Cabinet Assembly
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作者 Albert Eff Michael S. J. Walter 《Journal of Computer and Communications》 2024年第2期156-172,共17页
In times of digitalisation, visual assistance systems in assembly are increasingly important. The design of these assembly systems needs to be highly complex to meet the requirements. Due to the increasing number of v... In times of digitalisation, visual assistance systems in assembly are increasingly important. The design of these assembly systems needs to be highly complex to meet the requirements. Due to the increasing number of variants in production processes, as well as shorter innovation and product life cycles, assistance systems should improve quality and reduce complexity of assembly processes. However, many large kitchen manufacturers still assemble kitchen cabinets manually, due to the high variety of components, such as rails and fittings. This paper focuses on the analysis and evaluation of virtual assistance systems to improve quality and usability in individualised kitchen cabinet assembly processes at a large German manufacturer. A solution is identified and detailed. 展开更多
关键词 Digitalisation assembly Process Visual Assistance USABILITY Human-Centred assembly Design
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Target-induced Trivalent G-quadruplex/hemin DNAzyme for Colorimetric Detection of Hg^(2+) Based on an Exonuclease III Assisted Catalytic Hairpin Assembly
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作者 Zhenghua LIU Zhonghai LI 《Agricultural Biotechnology》 2024年第1期51-57,共7页
Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly fo... Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal output.The developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)detection.Such a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal ions.This strategy shows the great potential for visual detection with portable type. 展开更多
关键词 G-quadruplex/hemin DNAzyme Multivalence Catalytic hairpin assembly Exonuclease III Signal amplification Colorimetric detection
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利用Gibson Assembly技术构建乙型肝炎病毒1.1倍体复制子 被引量:1
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作者 方琳琳 宋浏伟 +3 位作者 杨林 吴勇 袁权 夏宁邵 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2015年第3期324-330,共7页
乙型肝炎病毒(HBV)基因组各开放阅读框高度重叠,因此构建可在体外细胞模型中复制的HBV DNA质粒至少要包含1.1倍以上的HBV基因组.构建HBV1.1倍体复制子的传统克隆方法涉及多步DNA酶切/连接,操作复杂,耗时较长.本研究发展了基于新型克隆技... 乙型肝炎病毒(HBV)基因组各开放阅读框高度重叠,因此构建可在体外细胞模型中复制的HBV DNA质粒至少要包含1.1倍以上的HBV基因组.构建HBV1.1倍体复制子的传统克隆方法涉及多步DNA酶切/连接,操作复杂,耗时较长.本研究发展了基于新型克隆技术Gibson Assembly构建HBV1.1倍体复制子质粒的新方法,在获得HBV基因组DNA后仅需1步体外装配即可完成,利用这一方法,本研究成功从4份HBV感染者标本中获得HBV1.1倍体复制子,并通过瞬时转染Huh7细胞,证明其能够支持HBV的体外复制.本研究为从临床标本中快速获得HBV复制子克隆以研究病毒表型功能提供了更为高效的新方法. 展开更多
关键词 乙型肝炎病毒 GIBSON assembly 1.1倍体
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应用Gibson Assembly方法构建狂犬病病毒SRV9株重组感染性cDNA克隆 被引量:1
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作者 王伟 魏玉圆 +3 位作者 翟少华 毛丽萍 皮文辉 简子健 《动物医学进展》 北大核心 2017年第11期11-17,共7页
应用Gibson Assembly连接法精确快速构建狂犬病病毒SRV9株重组感染性cDNA克隆,为狂犬病病毒感染性cDNA克隆的构建提供新的方法。应用Gibson Assembly连接法在同一反应体系内,将多个片段和经限制性内切酶线性化的载体,按设计的顺序进行连... 应用Gibson Assembly连接法精确快速构建狂犬病病毒SRV9株重组感染性cDNA克隆,为狂犬病病毒感染性cDNA克隆的构建提供新的方法。应用Gibson Assembly连接法在同一反应体系内,将多个片段和经限制性内切酶线性化的载体,按设计的顺序进行连接,实现多个片段的一步组装。设计带有20bp^30bp重叠序列的PCR引物,扩增得到带有重叠序列的狂犬病病毒5个结构蛋白基因和增强型荧光蛋白基因。扩增的片段和线性化的载体经胶回收纯化后,与Gibson连接液混合后,50℃反应60min,连接产物转化Stbl3感受态细胞,提取重组质粒,经PCR、酶切和测序鉴定,缺失了病毒伪基因Ψ区和糖蛋白基因的跨膜区并且将增强型荧光蛋白基因插入糖蛋白基因终止密码子前,构建了全长17 600bp的重组质粒,完成了重组全长感染性cDNA克隆的构建。 展开更多
关键词 狂犬病病毒 GIBSON assembly连接法 构建 感染性cDNA克隆
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Haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy with surface heparinization using electrostatic self assembly technology 被引量:8
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作者 余森 于振涛 +3 位作者 韩建业 WANG Gui 牛金龙 Matthew S.Dargusch 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第12期3046-3052,共7页
The haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy was studied after surface heparinization. A layer of sol-gel TiO2 films was applied on the alloy samples followed by active treatment in the bio-functiona... The haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy was studied after surface heparinization. A layer of sol-gel TiO2 films was applied on the alloy samples followed by active treatment in the bio-functionalized solution for introducing the OH- and groups, and then the heparin was immobilized on the active TiO2 films through the electrostatic self assembly technology. It is shown that the heparinized films are mainly composed of anatase and rutile with smooth and dense surface. In vitro blood compatibility was evaluated by haemolysis test, clotting time and platelet adhesion behavior tests. The results show that the haemocompatibility of the alloy could be significantly improved by surface heparinization. 展开更多
关键词 Ti-3Zr-2Sn-3Mo-25Nb alloy HAEMOCOMPATIBILITY HEPARINIZATION surface modification electrostatic self assembly
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CNC SYSTEM OF FLEXIBLE FIXTURE IN AIRCRAFT COMPONENT MANUFACTURING AND ASSEMBLY 被引量:2
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作者 郇极 靳阳 肖文磊 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2012年第1期54-61,共8页
The computer numerical control(CNC) system is suited to control varied types of flexible fixtures in aircraft component manufacturing and assembly. The mechanisms and control requirements of flexible fixtures are pr... The computer numerical control(CNC) system is suited to control varied types of flexible fixtures in aircraft component manufacturing and assembly. The mechanisms and control requirements of flexible fixtures are presented and analyzed. The hardware and software architecture and implementation of CNC system are pro- posed. The flexible fixture mechanism is described using configuration parameters. According to the parameters, the CNC system automatically generates the control feature and the human machine interface (HMI) operation function. The CNC system is implemented in a flexible fixture for skin-strlnger assembly, and results show the effectiveness of the system. 展开更多
关键词 computer numerical control(CNC) motion control aircraft assembly flexible fixture
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A STEP Based Integrated System for Assembly Planning and Design
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作者 殷晨波 钟秉林 易红 《Journal of Southeast University(English Edition)》 EI CAS 1999年第1期94-100,共7页
An integrated system for assembly planning and design (INSAPS) is presented in which product data can be exchanged between assembly planning and assembly design on the basis of standard for exchange of product data mo... An integrated system for assembly planning and design (INSAPS) is presented in which product data can be exchanged between assembly planning and assembly design on the basis of standard for exchange of product data model (STEP). The system consists of CAD system, product modeling system, assembly planning system. The product model is organized according to the STEP, uses mostly the entities of IR (integrated resources) and partly self defined entities which is necessary for assembly planning. A simple method of assembly sequence generation is presented which is suitable for complex assembly planning. The generated assembly sequences are evaluated considering the optimization of total assembly time. The results of assembly planning are feedback to the stage of assembly design to improve design. 展开更多
关键词 assembly model assembly planning production schedule STEP
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