Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous R...Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous Region of China. The purpose of this study was to determine the genetic structure and genotypic diversity among the different eco-geographical populations. Based on the results from this study, 8 pairs of fluorescent-AFLP primers showed clear electrophoregram and high polymorphism amongst the 64 pairs of EcoR Ⅰ/Mse Ⅰ (Mse Ⅰ - a FAM fluorescent marked primer) primers screened. There was a significant polymorphic difference for the same primer pair in different populations and for the same population with different primer pairs. The percentage of polymorphic loci (P) at species level was higher than Kuche, Hetian, Kashi population levels, respectively. The Nei's gene diversity index (H) and Shannon's information index (I) at species level were higher than those of Kuche, Hetian, and Kashi at population level, respectively. H and I of Kuche population were the highest amongst the three populations. Apricot population genetic diversity was found mainly within the population, Genetic differentiation coefficient between populations (GST) was 0.0882. Gene flow Nm between the populations was 5.1689. Population genetic identity was between 0.9772-0.9811 and genetic distance was between 0.0191-0.0232. These results further indicated that the similarity between populations was higher and the genetic distance between populations was smaller. The UPGMA cluster analysis indicates that the geographical populations at Kuche, Kashi, Hetian were relatively independent Mendelian populations. Concurrently, there was also partial gene exchange between the populations. All the evidences indicated that the genetic diversity in Kuche population was the highest, suggesting that it could be a transition population from wild apricot to cultivated apricot. There were abundant genetic diversities in apricot cultivar populations in southern Xinjiang, China, which provide promising germplasm for further breeding and theoretical basis for biodiversity conservation and utilization for apricot population in this area.展开更多
A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot (Prunus armeniaca) were buried at the soil depths of 0-cm,...A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot (Prunus armeniaca) were buried at the soil depths of 0-cm, 4-cm, 8-cm, and 12-cm, respectively, to simulate the seed hoarding behavior of rodents in the field. The results revealed that the rates of seed germination and established seedlings from buried seeds were both the highest in 4-cm burial depth group, and then decreased with increasing soil depth. The number of rotten seeds increased in deeper burial depth. It is unfavourable for seed germination at 0-cm burial depth (i.e., seeds were laid on soil surface). There was insignificant effect of burial depth on growth of established seedlings. The results from this study indicated that proper burial depth in soil would be helpful for the seed germination and seedling growth. The seedlings derived from buried seeds at shallower depth (4 cm) in this research have advantage in their early development.展开更多
[Objective] The aim of this study was to explore the daily change of photosyntheticratefor Prunus domestica ×armeniacain different growing seasons. The study can provide theoretical basis for arid area high yield...[Objective] The aim of this study was to explore the daily change of photosyntheticratefor Prunus domestica ×armeniacain different growing seasons. The study can provide theoretical basis for arid area high yield and quality cultivation.[Method] The photosynthetic physiological properties of leaves of different types of Prunus domestica × armeniaca were measured by the Li-6400 portable photosynthesis system indifferent seasons. By this method could analysis of photosyntheticcharacteristicsfor different types of Prunus domestica×armeniaca in different seasons.[Result] Daily change of photosyntheticrate(Pn) for Prunus domestica×armeniaca in differentseasons showed a "double-peak" curve. The peak values were at 10:00 and16:00. The Pn of ‘Fengweihuanghou', ‘Konglongdan', ‘Weihou', ‘Weiwang' and‘Weidi' reached the maximum in July, theywere 13.75, 14.76, 12.96, 13.3, and 11.9μmol/(m^2·s), respectively. The Pn of Prunus domestica×armeniaca reached minimumin August, they were 9.78, 10.71, 12.02, 10.43 μmol/(m^2·s). The Pn overall average of ‘Konglongdan' was highest,it reached 12.65 μmol/(m^2·s).The Pn overall average of ‘Weiwang' was lowest, it reached 11.31μmol/(m^2·s). There were extremely significant positive correlation between the Pn and Gs(P0.01). [Conclusion] Daily change of photosyntheticrate for Prunus domestica ×armeniaca in differentseasons showed a "double-peak" curve, showing significant phenomenon of "midday depression".The photosynthesis intensity of Prunus domestica ×armeniaca was strongest in July, and the photosynthesis intensity was weakest in August. ‘Konglongdan'showed the strongest photosynthesis capacity, ‘Weihou' and ‘Weiwang', followed.There were highest correlation between the Pn and stoma conductance(Gs).展开更多
[Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] ...[Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] Taking three varieties of Prunus domestica ×armeniaca as the research object, the phenophase of blooming,morphology characteristics of floral organs and development characteristics of floral organs were observed, pollen amount, pollen viability and stigma receptivity were measured. [Result] The results showed that three varieties of Prunus domestica×armeniaca were in blossom in early April, the florescence were relatively late for 8-12 d comparing with the varieties of apricot, the florescence were relatively similar to the P. domestica L. Morphology characteristics of floral organs of three varieties of Prunus domestica×armeniaca had few differences, were relatively similar to two varieties of plum, and they all had high percent of middle-style and long-style flower.The pollen amount of three varieties of Prunus domestica ×armeniaca had a little differences comparing with two varieties of plum, but were lower than three varieties of apricot. There were some differences in pollen viability, the germination percentage of pollen of ‘Konglongdan' was relatively high, was 51.6%; The pollen amount of ‘Fengweihuanghou' was relatively high, was 880.8 per anther; ‘Weidi' had fewer pollen amount, and the pollen viability was just 2.1%; Three varieties of Prunus domestica ×armeniaca all had strong stigma receptivity on the bloom day, and the stigma receptivity continued exist until the day that 5 days after blooming. [Conclusion] The florescence of three varieties of Prunus domestica ×armeniaca were relatively concentrated, and the rate of pistil abortion was lowest, the variation intensity of stigma receptivity and sustainable time of three varieties of Prunus domestica×armeniaca almost had a little differences comparing with a variety of plum, but slightly more than three varieties of apricot.展开更多
[Objective] This study aimed to investigate the photosynthetic characteristics of different Prunus domestica × armeniaca cultivars, thereby providing some theoretical basis for high-yield and high-quality cultiva...[Objective] This study aimed to investigate the photosynthetic characteristics of different Prunus domestica × armeniaca cultivars, thereby providing some theoretical basis for high-yield and high-quality cultivation of P. domestica × armeniaca. [Method] Total three cultivars(Konglongdan, Fengwei Huanghou and Weiwang)of P. domestica × armeniaca were selected as the test materials, and their leafphotosynthetic parameters were measured with Li-6400 portable photosynthesis system.The light response curve and CO_2 response curve were drawn. [Result] There were significant differences in photosynthetic rate among the three cultivars of P. domestica × armeniaca(P〈0.01). The photosynthetic rate of Konglongdan [14.76 μmol/(m^2·s)]was highest. When the light intensity reached 1 800 μmol/(m^2·s), the maximum net photosynthetic rate of Konglongdan was up to 27.27 μmol/(m^2·s), and its apparent quantum yield reached the peak. Konglongdan showed lower light compensation point [43.069 μmol/(m^2·s)] and higher light saturation point [697.684 μmol/(m^2·s)].Weiwang showed the lowest CO2 compensation point(68.264 μmol/mol) and the highest carboxylic efficiency [0.072 μmol/(m^2·s)]. [Conclusion] Konglongdan showed the highest light utilization efficiency and photosynthetic potential, and it had the strongest utilization ability for weak light. The utilization ability of Weiwang for lowconcentration CO2 was strongest, followed by those of Fengwei Huanghou and Konglongdan.展开更多
The genus Armeniaca Scop. is well known for its popular cultivated edible fruit trees such as Armeniaca vul- garis Lam. and ornamental flowers such as A. mume Sieb. Another species, A. cathayana Fu et al., one of six ...The genus Armeniaca Scop. is well known for its popular cultivated edible fruit trees such as Armeniaca vul- garis Lam. and ornamental flowers such as A. mume Sieb. Another species, A. cathayana Fu et al., one of six important dry fruits (kernel-using apricot), is cultivated for its edible seeds in North China. In the present study, DNA from 70 individuals of A rmeniaca, including 38 of A. cathayana, 18 ofA. vulgaris, 12 ofA. sibirica, 1 ofA. dasycarpa and 1 of A. mume, was extracted and analyzed using microsatellites and capillary electrophoresis. For 20 polymorphic loci selected, 339 alleles and 140.7 effective alleles were detected. The number of alleles per locus ranged from 8 to 28, with an average of 16.95 alleles per locus. The observed heterozygosity (Ho) and the expected heterozygosity (He) ranged from 0.427 to 0.971 and from 0.737 to 0.912, respectively. The polymorphism information content varied from 0.708 to0.905, with an average of 0.827. Based on the genetic similarity among 70 individuals, a UPGMA was used to establish the phylogenetic relationships. The taxonomic positions among five species were clearly revealed, and A. cathayana was more closely related to A. vulgaris than to A. sibirica. The results will provide a scientific basis for research on the taxonomy, germplasm resources and breeding ofArmeniaca, especially for A. cathayana.展开更多
[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca ( apricot ) Katy and...[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca ( apricot ) Katy and Prunus salicina (plum) Moriteni were analyzed by means of RAPD and SSR molecular markers. [ Result] We found the differences in the electrophoresis bands between progenies and their parents, indicating that hybrid seedlings obtained in this study were real. [ Conclusion ] The combination of the above methods is effective and fast to identify the hybrids, and can shorten the breeding period.展开更多
Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of ...Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.展开更多
[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. O...[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 (34) was conducted with four factors (Mg2+ , dNTPs, primers, Taq DNA polymerase) in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established : the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.展开更多
[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,an...[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.展开更多
基金This work was supported by the National Natural Science Foundation (No. 30471196)the Special Program for Doctorial Site of Universities (No. 200404344011).
文摘Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous Region of China. The purpose of this study was to determine the genetic structure and genotypic diversity among the different eco-geographical populations. Based on the results from this study, 8 pairs of fluorescent-AFLP primers showed clear electrophoregram and high polymorphism amongst the 64 pairs of EcoR Ⅰ/Mse Ⅰ (Mse Ⅰ - a FAM fluorescent marked primer) primers screened. There was a significant polymorphic difference for the same primer pair in different populations and for the same population with different primer pairs. The percentage of polymorphic loci (P) at species level was higher than Kuche, Hetian, Kashi population levels, respectively. The Nei's gene diversity index (H) and Shannon's information index (I) at species level were higher than those of Kuche, Hetian, and Kashi at population level, respectively. H and I of Kuche population were the highest amongst the three populations. Apricot population genetic diversity was found mainly within the population, Genetic differentiation coefficient between populations (GST) was 0.0882. Gene flow Nm between the populations was 5.1689. Population genetic identity was between 0.9772-0.9811 and genetic distance was between 0.0191-0.0232. These results further indicated that the similarity between populations was higher and the genetic distance between populations was smaller. The UPGMA cluster analysis indicates that the geographical populations at Kuche, Kashi, Hetian were relatively independent Mendelian populations. Concurrently, there was also partial gene exchange between the populations. All the evidences indicated that the genetic diversity in Kuche population was the highest, suggesting that it could be a transition population from wild apricot to cultivated apricot. There were abundant genetic diversities in apricot cultivar populations in southern Xinjiang, China, which provide promising germplasm for further breeding and theoretical basis for biodiversity conservation and utilization for apricot population in this area.
基金supported by National Basic Research Program of China (No. 2007CB109106)by the Zoology Key Subject Fund of Henan Province
文摘A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot (Prunus armeniaca) were buried at the soil depths of 0-cm, 4-cm, 8-cm, and 12-cm, respectively, to simulate the seed hoarding behavior of rodents in the field. The results revealed that the rates of seed germination and established seedlings from buried seeds were both the highest in 4-cm burial depth group, and then decreased with increasing soil depth. The number of rotten seeds increased in deeper burial depth. It is unfavourable for seed germination at 0-cm burial depth (i.e., seeds were laid on soil surface). There was insignificant effect of burial depth on growth of established seedlings. The results from this study indicated that proper burial depth in soil would be helpful for the seed germination and seedling growth. The seedlings derived from buried seeds at shallower depth (4 cm) in this research have advantage in their early development.
基金Supported by High-quality and High-efficient Cultivation Technology Demonstration and Promotion of Apricot and Plum(ZYLYKJTG2015020)~~
文摘[Objective] The aim of this study was to explore the daily change of photosyntheticratefor Prunus domestica ×armeniacain different growing seasons. The study can provide theoretical basis for arid area high yield and quality cultivation.[Method] The photosynthetic physiological properties of leaves of different types of Prunus domestica × armeniaca were measured by the Li-6400 portable photosynthesis system indifferent seasons. By this method could analysis of photosyntheticcharacteristicsfor different types of Prunus domestica×armeniaca in different seasons.[Result] Daily change of photosyntheticrate(Pn) for Prunus domestica×armeniaca in differentseasons showed a "double-peak" curve. The peak values were at 10:00 and16:00. The Pn of ‘Fengweihuanghou', ‘Konglongdan', ‘Weihou', ‘Weiwang' and‘Weidi' reached the maximum in July, theywere 13.75, 14.76, 12.96, 13.3, and 11.9μmol/(m^2·s), respectively. The Pn of Prunus domestica×armeniaca reached minimumin August, they were 9.78, 10.71, 12.02, 10.43 μmol/(m^2·s). The Pn overall average of ‘Konglongdan' was highest,it reached 12.65 μmol/(m^2·s).The Pn overall average of ‘Weiwang' was lowest, it reached 11.31μmol/(m^2·s). There were extremely significant positive correlation between the Pn and Gs(P0.01). [Conclusion] Daily change of photosyntheticrate for Prunus domestica ×armeniaca in differentseasons showed a "double-peak" curve, showing significant phenomenon of "midday depression".The photosynthesis intensity of Prunus domestica ×armeniaca was strongest in July, and the photosynthesis intensity was weakest in August. ‘Konglongdan'showed the strongest photosynthesis capacity, ‘Weihou' and ‘Weiwang', followed.There were highest correlation between the Pn and stoma conductance(Gs).
基金Supported by Highly-qualified and Highly-efficient Cultivation Technology Demonstration adn Promotion of Apricot and Plum~~
文摘[Objective] The aims of this study were to understand the flowering biological characteristics of Prunus domestica ×armeniaca and analyze its differences with other apricot varieties and plum varieties. [Method] Taking three varieties of Prunus domestica ×armeniaca as the research object, the phenophase of blooming,morphology characteristics of floral organs and development characteristics of floral organs were observed, pollen amount, pollen viability and stigma receptivity were measured. [Result] The results showed that three varieties of Prunus domestica×armeniaca were in blossom in early April, the florescence were relatively late for 8-12 d comparing with the varieties of apricot, the florescence were relatively similar to the P. domestica L. Morphology characteristics of floral organs of three varieties of Prunus domestica×armeniaca had few differences, were relatively similar to two varieties of plum, and they all had high percent of middle-style and long-style flower.The pollen amount of three varieties of Prunus domestica ×armeniaca had a little differences comparing with two varieties of plum, but were lower than three varieties of apricot. There were some differences in pollen viability, the germination percentage of pollen of ‘Konglongdan' was relatively high, was 51.6%; The pollen amount of ‘Fengweihuanghou' was relatively high, was 880.8 per anther; ‘Weidi' had fewer pollen amount, and the pollen viability was just 2.1%; Three varieties of Prunus domestica ×armeniaca all had strong stigma receptivity on the bloom day, and the stigma receptivity continued exist until the day that 5 days after blooming. [Conclusion] The florescence of three varieties of Prunus domestica ×armeniaca were relatively concentrated, and the rate of pistil abortion was lowest, the variation intensity of stigma receptivity and sustainable time of three varieties of Prunus domestica×armeniaca almost had a little differences comparing with a variety of plum, but slightly more than three varieties of apricot.
基金Supported by Demonstration and Extension Project for High-quality and High-efficiency Cultivation Techniques of Prunus domestica×armeniaca(ZYLYKJTG2015020)~~
文摘[Objective] This study aimed to investigate the photosynthetic characteristics of different Prunus domestica × armeniaca cultivars, thereby providing some theoretical basis for high-yield and high-quality cultivation of P. domestica × armeniaca. [Method] Total three cultivars(Konglongdan, Fengwei Huanghou and Weiwang)of P. domestica × armeniaca were selected as the test materials, and their leafphotosynthetic parameters were measured with Li-6400 portable photosynthesis system.The light response curve and CO_2 response curve were drawn. [Result] There were significant differences in photosynthetic rate among the three cultivars of P. domestica × armeniaca(P〈0.01). The photosynthetic rate of Konglongdan [14.76 μmol/(m^2·s)]was highest. When the light intensity reached 1 800 μmol/(m^2·s), the maximum net photosynthetic rate of Konglongdan was up to 27.27 μmol/(m^2·s), and its apparent quantum yield reached the peak. Konglongdan showed lower light compensation point [43.069 μmol/(m^2·s)] and higher light saturation point [697.684 μmol/(m^2·s)].Weiwang showed the lowest CO2 compensation point(68.264 μmol/mol) and the highest carboxylic efficiency [0.072 μmol/(m^2·s)]. [Conclusion] Konglongdan showed the highest light utilization efficiency and photosynthetic potential, and it had the strongest utilization ability for weak light. The utilization ability of Weiwang for lowconcentration CO2 was strongest, followed by those of Fengwei Huanghou and Konglongdan.
基金financially supported by 948 Project(No.2011-4-37)Standardization Project(No.2013-LY-082) of The State Forestry Administration of China
文摘The genus Armeniaca Scop. is well known for its popular cultivated edible fruit trees such as Armeniaca vul- garis Lam. and ornamental flowers such as A. mume Sieb. Another species, A. cathayana Fu et al., one of six important dry fruits (kernel-using apricot), is cultivated for its edible seeds in North China. In the present study, DNA from 70 individuals of A rmeniaca, including 38 of A. cathayana, 18 ofA. vulgaris, 12 ofA. sibirica, 1 ofA. dasycarpa and 1 of A. mume, was extracted and analyzed using microsatellites and capillary electrophoresis. For 20 polymorphic loci selected, 339 alleles and 140.7 effective alleles were detected. The number of alleles per locus ranged from 8 to 28, with an average of 16.95 alleles per locus. The observed heterozygosity (Ho) and the expected heterozygosity (He) ranged from 0.427 to 0.971 and from 0.737 to 0.912, respectively. The polymorphism information content varied from 0.708 to0.905, with an average of 0.827. Based on the genetic similarity among 70 individuals, a UPGMA was used to establish the phylogenetic relationships. The taxonomic positions among five species were clearly revealed, and A. cathayana was more closely related to A. vulgaris than to A. sibirica. The results will provide a scientific basis for research on the taxonomy, germplasm resources and breeding ofArmeniaca, especially for A. cathayana.
基金Supported by the Natural Science Foundation of Shandong Province(Q2008D01)Program for Science Research Development of Higher Education Institutions of Shandong Province(J09LC54)
文摘[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca ( apricot ) Katy and Prunus salicina (plum) Moriteni were analyzed by means of RAPD and SSR molecular markers. [ Result] We found the differences in the electrophoresis bands between progenies and their parents, indicating that hybrid seedlings obtained in this study were real. [ Conclusion ] The combination of the above methods is effective and fast to identify the hybrids, and can shorten the breeding period.
基金This work was supported by National Natural Science Foundation of China(No.81360684)Guangxi Key Research and Development Plan Project(Gui Ke AB18221095)+3 种基金Teaching Teacher Training Project from Youjiang Medical University for Nationalities-National Teaching Teacher Training Project(You Hospital Zi[2018]No.98)High-level Talent Research Projects from Youjiang Medical University For Nationalities(No.01002018079)China National and Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599022)China Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599073).
文摘Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.
基金Supported by Start-up Fund for High-level Scientific Research Personnel(11GK30)
文摘[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 (34) was conducted with four factors (Mg2+ , dNTPs, primers, Taq DNA polymerase) in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established : the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.
文摘[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.