Objective:To establish a collagen type II-induced rat model of rheumatoid arthritis(RA)presenting characteristics of the human form of the traditional Chinese syndrome pattern of toxic heat-stasis painful obstruction(...Objective:To establish a collagen type II-induced rat model of rheumatoid arthritis(RA)presenting characteristics of the human form of the traditional Chinese syndrome pattern of toxic heat-stasis painful obstruction(bi zheng;arthromyodynia)as well as pathologic features of active RA.The Chinese herbal medicine Tengmei decoction was used to validate the animal model.Methods:Ninety specific pathogen free Sprague-Dawley rats were randomly divided into a normal group of 6 rats and a model group of 84 rats.To establish the rat model of collageninduced arthritis(CIA),bovine type II collagen in complete Freund’s adjuvant was injected into the model group rats as a priming dose(Day 0)and boosting dose(Day 9).Changes in arthritic index(AI)scores,including limb swelling,were monitored.Thereafter,24 successfullyestablished CIA rats were randomly assigned to 4 groups with 6 animals each:model,positive control drug,high-dose traditional Chinese herbal medicine,and traditional Chinese herbal medicine.A blank control group of 6 rats was included.After 12 weeks of intervention with Tengmei decoction,articular synovial tissue and serum specimens were collected to detect interleukin-2(IL-2)and IL-17 transcription and protein expression levels.展开更多
Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and ther...Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and therefore early diagnosis and evaluation of the disease is usually difficult. In this study, an arthritis model was successfully induced by injecting complete Freund’s adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injection of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accompanied by more sensitivity to thermal stimulation. Using the ultraweak bio-photon imaging system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7 days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats showed significantly higher biophoton emissions compared with the control group. The results of this study may provide a basis for further research and devel-opment of early diagnosis and assessment of lesion progression of rheumatoid arthritis.展开更多
目的利用完全弗氏佐剂(CFA)建立雌性SD大鼠类风湿关节炎(RA)模型,并考察不同给药方式的塞来昔布对RA的治疗效果。方法将24只雌性SD大鼠随机分成正常对照组和低、中、高剂量模型组(n=6),低、中、高剂量模型组分别用0.05、0.1、0.2 mL CFA...目的利用完全弗氏佐剂(CFA)建立雌性SD大鼠类风湿关节炎(RA)模型,并考察不同给药方式的塞来昔布对RA的治疗效果。方法将24只雌性SD大鼠随机分成正常对照组和低、中、高剂量模型组(n=6),低、中、高剂量模型组分别用0.05、0.1、0.2 mL CFA(10 mg/mL)于大鼠右后肢足跖部注射诱导构建RA模型,筛选出合适的剂量。另取24只雌性SD大鼠分为正常对照组、模型对照组、塞来昔布口服组、塞来昔布注射组(n=6),后3组利用最佳致炎剂量CFA建立RA模型。塞来昔布口服组给予市售塞来昔布胶囊,每天灌胃1次,每次20 mg;塞来昔布注射组给予用原料药配制的塞来昔布混悬液,每周于大鼠右后肢足跖部注射给药1次,剂量20 mg/kg。测量各组大鼠足容积,并进行临床评分。通过ELISA检测各组大鼠血清中3种炎症因子(IL-1β、IL-6、TNF-α)的浓度,取大鼠踝关节组织进行H-E染色以观察组织病变情况。结果在雌性SD大鼠中,足跖部注射0.1 mL CFA(10 mg/mL)可较好地模拟RA中度炎症反应,注射CFA后逐渐出现足爪红肿,全身临床评分及足部关节炎指数评分均有升高,炎症高峰期在免疫后19 d。塞来昔布口服组和注射组足容积较模型对照组减小(P均<0.05),全身临床评分及足部关节炎指数评分均较模型对照组降低(P均<0.01),血清IL-1β、IL-6、TNF-α浓度均较模型对照组降低(P均<0.05),踝关节组织病理改变均较模型对照组减轻;其中塞来昔布注射组的全身临床评分及足部关节炎指数评分低于口服组(P均<0.05),踝关节组织病理改变轻于口服组。结论足跖部注射0.1 mL CFA(10 mg/mL)可致SD雌性大鼠中度炎症反应,能较好地模拟RA的病理过程。每周经足跖部注射1次塞来昔布混悬液对模型大鼠RA的疗效优于每天口服1次塞来昔布。展开更多
文摘Objective:To establish a collagen type II-induced rat model of rheumatoid arthritis(RA)presenting characteristics of the human form of the traditional Chinese syndrome pattern of toxic heat-stasis painful obstruction(bi zheng;arthromyodynia)as well as pathologic features of active RA.The Chinese herbal medicine Tengmei decoction was used to validate the animal model.Methods:Ninety specific pathogen free Sprague-Dawley rats were randomly divided into a normal group of 6 rats and a model group of 84 rats.To establish the rat model of collageninduced arthritis(CIA),bovine type II collagen in complete Freund’s adjuvant was injected into the model group rats as a priming dose(Day 0)and boosting dose(Day 9).Changes in arthritic index(AI)scores,including limb swelling,were monitored.Thereafter,24 successfullyestablished CIA rats were randomly assigned to 4 groups with 6 animals each:model,positive control drug,high-dose traditional Chinese herbal medicine,and traditional Chinese herbal medicine.A blank control group of 6 rats was included.After 12 weeks of intervention with Tengmei decoction,articular synovial tissue and serum specimens were collected to detect interleukin-2(IL-2)and IL-17 transcription and protein expression levels.
文摘Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and therefore early diagnosis and evaluation of the disease is usually difficult. In this study, an arthritis model was successfully induced by injecting complete Freund’s adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injection of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accompanied by more sensitivity to thermal stimulation. Using the ultraweak bio-photon imaging system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7 days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats showed significantly higher biophoton emissions compared with the control group. The results of this study may provide a basis for further research and devel-opment of early diagnosis and assessment of lesion progression of rheumatoid arthritis.
文摘目的利用完全弗氏佐剂(CFA)建立雌性SD大鼠类风湿关节炎(RA)模型,并考察不同给药方式的塞来昔布对RA的治疗效果。方法将24只雌性SD大鼠随机分成正常对照组和低、中、高剂量模型组(n=6),低、中、高剂量模型组分别用0.05、0.1、0.2 mL CFA(10 mg/mL)于大鼠右后肢足跖部注射诱导构建RA模型,筛选出合适的剂量。另取24只雌性SD大鼠分为正常对照组、模型对照组、塞来昔布口服组、塞来昔布注射组(n=6),后3组利用最佳致炎剂量CFA建立RA模型。塞来昔布口服组给予市售塞来昔布胶囊,每天灌胃1次,每次20 mg;塞来昔布注射组给予用原料药配制的塞来昔布混悬液,每周于大鼠右后肢足跖部注射给药1次,剂量20 mg/kg。测量各组大鼠足容积,并进行临床评分。通过ELISA检测各组大鼠血清中3种炎症因子(IL-1β、IL-6、TNF-α)的浓度,取大鼠踝关节组织进行H-E染色以观察组织病变情况。结果在雌性SD大鼠中,足跖部注射0.1 mL CFA(10 mg/mL)可较好地模拟RA中度炎症反应,注射CFA后逐渐出现足爪红肿,全身临床评分及足部关节炎指数评分均有升高,炎症高峰期在免疫后19 d。塞来昔布口服组和注射组足容积较模型对照组减小(P均<0.05),全身临床评分及足部关节炎指数评分均较模型对照组降低(P均<0.01),血清IL-1β、IL-6、TNF-α浓度均较模型对照组降低(P均<0.05),踝关节组织病理改变均较模型对照组减轻;其中塞来昔布注射组的全身临床评分及足部关节炎指数评分低于口服组(P均<0.05),踝关节组织病理改变轻于口服组。结论足跖部注射0.1 mL CFA(10 mg/mL)可致SD雌性大鼠中度炎症反应,能较好地模拟RA的病理过程。每周经足跖部注射1次塞来昔布混悬液对模型大鼠RA的疗效优于每天口服1次塞来昔布。
