Defects in the function and development of GABAergic interneurons have been linked to psychiatric disorders, so preservation of these interneurons in brain slices is important for successful electrophysiological recor...Defects in the function and development of GABAergic interneurons have been linked to psychiatric disorders, so preservation of these interneurons in brain slices is important for successful electrophysiological recording in various ex vivo methods. However, it is difficult to maintain the activity and morphology of neurons in slices from mice of 〉30 days old. Here we evaluated the N-methyI-D-glucamine （NMDG）- based artificial cerebrospinal fluid （aCSF） method for the preservation of interneurons in slices from mice of up to -6 months old and discussed the steps that may affect their quality during slicing. We found that the NMDG-aCSF method rescued more cells than sucrose-aCSF and successfully preserved different types of interneurons including parvalbumin- and somatostatin-positive interneurons. In addition, both the chemical and electrical synaptic signaling of interneurons were maintained. These results demonstrate that the NMDG-aCSF method is suitable for the preservation of interneurons, especially in studies of gap junctions.展开更多
目的比较不同种类的冲洗液以及冲洗液温度对慢性硬膜下血肿(cSDH)颅骨钻孔冲洗引流术后复发的影响。方法检索PubMed、Embase、Cochrane Library、Web of science、CNKI、维普数据库,查找颅骨钻孔冲洗引流术治疗cSDH的研究,应用Meta方法...目的比较不同种类的冲洗液以及冲洗液温度对慢性硬膜下血肿(cSDH)颅骨钻孔冲洗引流术后复发的影响。方法检索PubMed、Embase、Cochrane Library、Web of science、CNKI、维普数据库,查找颅骨钻孔冲洗引流术治疗cSDH的研究,应用Meta方法分析不同种类的冲洗液以及冲洗液温度对cSDH颅骨钻孔冲洗引流术后复发的影响。结果共纳入9项研究2033例病人,其中有6项研究1175例病人涉及复发情况,3项研究858例病人涉及冲洗液温度对cSDH复发的影响。Meta分析显示,人工脑脊液组(n=576)术后复发率低于生理盐水组(n=599)(OR=0.41,95%CI=0.20~0.85,P=0.016),体温组(n=432)术后复发率明显低于室温组(n=426)(OR=2.92,95%CI=1.92~4.42,P<0.001)。结论cSDH颅骨钻孔冲洗引流术中,人工脑脊液体温冲洗能降低cSDH术后复发率。展开更多
Previous studies have demonstrated that differentiated neural stem cells （NSCs） are more suitable for transplantation than non-differentiated NSCs. In this study, NSCs were expanded in vitro for two passages, induce...Previous studies have demonstrated that differentiated neural stem cells （NSCs） are more suitable for transplantation than non-differentiated NSCs. In this study, NSCs were expanded in vitro for two passages, induced with retinoic acid to differentiate, and harvested between 1 6 days later. They were subsequently cultured in artificial cerebrospinal fluid for an additional 3 days, dudng which their growth and morphology was monitored. NSCs induced for 4 days exhibited a peak rate of cells differentiating into neurons and robust growth. Our results indicate that the optimal time point for transplanting NSCs is following a 4-day period of induced differentiation.展开更多
基金supported by grants from National Funds for Distinguished Young Scientists of China (81225007)the Funds for Creative Research Groups of China (81221003)
文摘Defects in the function and development of GABAergic interneurons have been linked to psychiatric disorders, so preservation of these interneurons in brain slices is important for successful electrophysiological recording in various ex vivo methods. However, it is difficult to maintain the activity and morphology of neurons in slices from mice of 〉30 days old. Here we evaluated the N-methyI-D-glucamine （NMDG）- based artificial cerebrospinal fluid （aCSF） method for the preservation of interneurons in slices from mice of up to -6 months old and discussed the steps that may affect their quality during slicing. We found that the NMDG-aCSF method rescued more cells than sucrose-aCSF and successfully preserved different types of interneurons including parvalbumin- and somatostatin-positive interneurons. In addition, both the chemical and electrical synaptic signaling of interneurons were maintained. These results demonstrate that the NMDG-aCSF method is suitable for the preservation of interneurons, especially in studies of gap junctions.
文摘目的比较不同种类的冲洗液以及冲洗液温度对慢性硬膜下血肿(cSDH)颅骨钻孔冲洗引流术后复发的影响。方法检索PubMed、Embase、Cochrane Library、Web of science、CNKI、维普数据库,查找颅骨钻孔冲洗引流术治疗cSDH的研究,应用Meta方法分析不同种类的冲洗液以及冲洗液温度对cSDH颅骨钻孔冲洗引流术后复发的影响。结果共纳入9项研究2033例病人,其中有6项研究1175例病人涉及复发情况,3项研究858例病人涉及冲洗液温度对cSDH复发的影响。Meta分析显示,人工脑脊液组(n=576)术后复发率低于生理盐水组(n=599)(OR=0.41,95%CI=0.20~0.85,P=0.016),体温组(n=432)术后复发率明显低于室温组(n=426)(OR=2.92,95%CI=1.92~4.42,P<0.001)。结论cSDH颅骨钻孔冲洗引流术中,人工脑脊液体温冲洗能降低cSDH术后复发率。
基金the National Natural Science Foundation of China, No. 30570628, 30770751
文摘Previous studies have demonstrated that differentiated neural stem cells （NSCs） are more suitable for transplantation than non-differentiated NSCs. In this study, NSCs were expanded in vitro for two passages, induced with retinoic acid to differentiate, and harvested between 1 6 days later. They were subsequently cultured in artificial cerebrospinal fluid for an additional 3 days, dudng which their growth and morphology was monitored. NSCs induced for 4 days exhibited a peak rate of cells differentiating into neurons and robust growth. Our results indicate that the optimal time point for transplanting NSCs is following a 4-day period of induced differentiation.
