Brassica rapa cuhivars Qinghaidahuang and Menyuan small rape were used as female parents and separately interspecifically hybridized with Zhongchijie- lan to artificially synthesize a number of Brasica napus resources...Brassica rapa cuhivars Qinghaidahuang and Menyuan small rape were used as female parents and separately interspecifically hybridized with Zhongchijie- lan to artificially synthesize a number of Brasica napus resources. Observation of meiosis, pollen fertility and pollen tube germination and analysis of self-compati- bility index of these artificial B. napus varieties were conducted. Results showed that pollen mother cells of artificial B. napus lines from two cross combinations all had normal chromosome behavior and high pollen fertility, but the selfing seed-setting and self-compatibility index varied extremely significantly; B. napus lines from cross combination Qinghaidahuang x Zhongchijidan are highly self-compatible, while B. napus lines from cross combination Menyuan small rape x Zhongchijielan are highly self-incompatible. This study analyzed the heredity laws of self-incompatibility trait in artificially synthesized lines and discussed applica- tion prospects of artificially synthesized self-incompatible B. napus lines in rapeseed production.展开更多
基金Supported by National Natural Science Foundation of China(30960200)National Science and Technology Support Program of China(2011BAD35B04)Applied Basic Research Project of Qinghai Province(2011-Z-701)
文摘Brassica rapa cuhivars Qinghaidahuang and Menyuan small rape were used as female parents and separately interspecifically hybridized with Zhongchijie- lan to artificially synthesize a number of Brasica napus resources. Observation of meiosis, pollen fertility and pollen tube germination and analysis of self-compati- bility index of these artificial B. napus varieties were conducted. Results showed that pollen mother cells of artificial B. napus lines from two cross combinations all had normal chromosome behavior and high pollen fertility, but the selfing seed-setting and self-compatibility index varied extremely significantly; B. napus lines from cross combination Qinghaidahuang x Zhongchijidan are highly self-compatible, while B. napus lines from cross combination Menyuan small rape x Zhongchijielan are highly self-incompatible. This study analyzed the heredity laws of self-incompatibility trait in artificially synthesized lines and discussed applica- tion prospects of artificially synthesized self-incompatible B. napus lines in rapeseed production.
