Polysaccharides are widely present in herbs with multiple activities,especially immunity regulation and metabolic benefits for metabolic disorders.However,the underlying mechanisms are not well under-stood.Functional ...Polysaccharides are widely present in herbs with multiple activities,especially immunity regulation and metabolic benefits for metabolic disorders.However,the underlying mechanisms are not well under-stood.Functional metabolomics is increasingly used to investigate systemic effects on the host by iden-tifying metabolites with particular functions.This study explores the mechanisms underlying the metabolic benefits of Astragalus polysaccharides(APS)by adopting a functional metabolomics strategy.The effects of APS were determined in eight-week high-fat diet(HFD)-fed obese mice.Then,gas chromatography–time-of-flight mass spectrometry(GC–TOFMS)-based untargeted metabolomics was performed for an analysis of serum and liver tissues,and liquid chromatography–tandem mass spectrom-etry(LC–MS/MS)-based targeted metabolomics was performed.The potential functions of the metabo-lites were tested with in vitro and in vivo models of metabolic disorders.Our results first confirmed the metabolic benefits of APS in obese mice.Then,metabolomics analysis revealed that APS supplemen-tation reversed the HFD-induced metabolic changes,and identified 2-hydroxybutyric acid(2-HB)as a potential functional metabolite for APS activity that was significantly decreased by a HFD and reversed by APS.Further study indicated that 2-HB inhibited oleic acid(OA)-induced triglyceride(TG)accumula-tion.It was also found to stimulate the expression of proteins in lipid degradation in hepatocytes and TG lipolysis in 3T3-L1 cells.Moreover,it was found to reduce serum TG and regulate the proteins involved in lipid degradation in high-fat and high-sucrose(HFHS)-fed mice.In conclusion,our study demonstrates that the metabolic benefits of APS are at least partially due to 2-HB generation,which modulated lipid metabolism both in vitro and in vivo.Our results also highlight that functional metabolomics is practical for investigating the mechanism underlying the systemic benefits of plant polysaccharides.展开更多
[Objectives]To explore the extraction and in vitro antioxidant effects of astragalus polysaccharides(APS)and Ganoderma lucidum mycelia polysaccharides(GLMPS).[Methods]By studying the polysaccharides of the herbal medi...[Objectives]To explore the extraction and in vitro antioxidant effects of astragalus polysaccharides(APS)and Ganoderma lucidum mycelia polysaccharides(GLMPS).[Methods]By studying the polysaccharides of the herbal medicinal material Astragalus membranaceus and the fungal medicinal material Ganoderma lucidum mycelia,two polysaccharides were mixed according to different proportions and concentrations by using the principle of traditional Chinese medicine compound combination.The effect of polysaccharides on the scavenging ability of hydroxyl radical system was determined by salicylic acid method.[Results]When the compound ratios of GLMPS and APS were 1∶1,1∶4,1∶5,4∶1,and 5∶1,the scavenging effect of compound polysaccharides was better than that of single-component polysaccharides,and with the increase of concentration,the scavenging effect increased.When the ratio of GLMPS and APS was 5∶1,the hydroxyl radical scavenging rate of the compound polysaccharide reached 59.77%,which was 18.72%higher than that of single GLMPS and 28.58%higher than that of single APS.The scavenging effect of compound polysaccharide is closely related to the compound ratio and concentration.[Conclusions]APS and GLMPS can obtain better hydroxyl radical scavenging ability than single-component polysaccharides through compounding in appropriate proportions.In addition,within a suitable concentration range,as the concentration increases,the scavenging ability also increases.展开更多
Adenophora polysaccharides (AP), is an active principle extracted from the root of Adenophorae Potaninii Korsh originated in Gansu Province and isolated with boiling water(1). AP is isolated and purified from the crud...Adenophora polysaccharides (AP), is an active principle extracted from the root of Adenophorae Potaninii Korsh originated in Gansu Province and isolated with boiling water(1). AP is isolated and purified from the crude drug by DEAE-cellulose and Sephadex G-200 column, with a white powder and mean molecular weight of 8. 3×104 , and [α]D20 of AP is +68(1). AP is only composed of glucose judging展开更多
Herpesviral hematopoietic necrosis disease caused by cyprinid herpesvirus 2(CyHV-2)results in huge economic losses for the gibel carp(Carassius auratus gibelio)aquaculture industry.A vaccination strategy is a feasible...Herpesviral hematopoietic necrosis disease caused by cyprinid herpesvirus 2(CyHV-2)results in huge economic losses for the gibel carp(Carassius auratus gibelio)aquaculture industry.A vaccination strategy is a feasible method to prevent CyHV-2 infection and the resulting economic losses.In this study,inactivators(includingβ-propiolactone,formaldehyde and binary ethylenimine)were used to prepare an inactivated vaccine.The optimal inactivated CyHV-2 vaccine(0.2%β-propiolactone inactivates CyHV-2 for 48 h)mixed withβ-glucan,anisodamine,chitosan,and astragalus polysaccharide(APS)adjuvants were injected into gibel carp.Theβ-glucan and APS combined with inactivated vaccine significantly improved the relative immune protection rate of gibel carp against CyHV-2 infection.The highest specific antibody levels inβ-glucan and APS groups were found in serum by ELISA assay and antibody neutralization titer.Furthermore,adjuvant addition groups produced better results for lysozyme,total superoxide dismutase,and complement C3 in serum.β-glucan and APS combined with vaccine treatment effectively enhanced mRNA expressions of important immune genes(IL-1β,IgM,IL-2 and IFN-γ2).Minimal tissue lesions(gill,spleen,and head kidney)and virus replication were seen in theβ-glucan and APS groups by histopathological examination and expression analysis of CyHV-2 TK gene.These results indicated that the combination of inactivated vaccine and adjuvantβ-glucan or APS is an effective method against CyHV-2 infection and provided a case study reference for prevention of fish viral diseases using inactivated vaccines.展开更多
基金funded by the National Natural Science Foundation of China (81673662 and 81873059)the Program for Professor of Special Appointment (Eastern Scholar)&Shuguang Scholar (16SG36) at the Shanghai Institutions of Higher Learning from Shanghai Municipal Education
文摘Polysaccharides are widely present in herbs with multiple activities,especially immunity regulation and metabolic benefits for metabolic disorders.However,the underlying mechanisms are not well under-stood.Functional metabolomics is increasingly used to investigate systemic effects on the host by iden-tifying metabolites with particular functions.This study explores the mechanisms underlying the metabolic benefits of Astragalus polysaccharides(APS)by adopting a functional metabolomics strategy.The effects of APS were determined in eight-week high-fat diet(HFD)-fed obese mice.Then,gas chromatography–time-of-flight mass spectrometry(GC–TOFMS)-based untargeted metabolomics was performed for an analysis of serum and liver tissues,and liquid chromatography–tandem mass spectrom-etry(LC–MS/MS)-based targeted metabolomics was performed.The potential functions of the metabo-lites were tested with in vitro and in vivo models of metabolic disorders.Our results first confirmed the metabolic benefits of APS in obese mice.Then,metabolomics analysis revealed that APS supplemen-tation reversed the HFD-induced metabolic changes,and identified 2-hydroxybutyric acid(2-HB)as a potential functional metabolite for APS activity that was significantly decreased by a HFD and reversed by APS.Further study indicated that 2-HB inhibited oleic acid(OA)-induced triglyceride(TG)accumula-tion.It was also found to stimulate the expression of proteins in lipid degradation in hepatocytes and TG lipolysis in 3T3-L1 cells.Moreover,it was found to reduce serum TG and regulate the proteins involved in lipid degradation in high-fat and high-sucrose(HFHS)-fed mice.In conclusion,our study demonstrates that the metabolic benefits of APS are at least partially due to 2-HB generation,which modulated lipid metabolism both in vitro and in vivo.Our results also highlight that functional metabolomics is practical for investigating the mechanism underlying the systemic benefits of plant polysaccharides.
基金Supported by the Project of"Striving to Be First-classImproving Weak LinksBuilding Strong Feature"in 2019–Tropical and South China Sea Biological Resources Comprehensive Utilization Program(000301900410)。
文摘[Objectives]To explore the extraction and in vitro antioxidant effects of astragalus polysaccharides(APS)and Ganoderma lucidum mycelia polysaccharides(GLMPS).[Methods]By studying the polysaccharides of the herbal medicinal material Astragalus membranaceus and the fungal medicinal material Ganoderma lucidum mycelia,two polysaccharides were mixed according to different proportions and concentrations by using the principle of traditional Chinese medicine compound combination.The effect of polysaccharides on the scavenging ability of hydroxyl radical system was determined by salicylic acid method.[Results]When the compound ratios of GLMPS and APS were 1∶1,1∶4,1∶5,4∶1,and 5∶1,the scavenging effect of compound polysaccharides was better than that of single-component polysaccharides,and with the increase of concentration,the scavenging effect increased.When the ratio of GLMPS and APS was 5∶1,the hydroxyl radical scavenging rate of the compound polysaccharide reached 59.77%,which was 18.72%higher than that of single GLMPS and 28.58%higher than that of single APS.The scavenging effect of compound polysaccharide is closely related to the compound ratio and concentration.[Conclusions]APS and GLMPS can obtain better hydroxyl radical scavenging ability than single-component polysaccharides through compounding in appropriate proportions.In addition,within a suitable concentration range,as the concentration increases,the scavenging ability also increases.
基金This project was supported by Natural Science Founda-tion of China(No:39730480)
文摘Adenophora polysaccharides (AP), is an active principle extracted from the root of Adenophorae Potaninii Korsh originated in Gansu Province and isolated with boiling water(1). AP is isolated and purified from the crude drug by DEAE-cellulose and Sephadex G-200 column, with a white powder and mean molecular weight of 8. 3×104 , and [α]D20 of AP is +68(1). AP is only composed of glucose judging
基金supported by Fundamental Research Funds for the Central Universities (2662021SCPY006)National Key Research and Development Program of China (2018YFD0900504).
文摘Herpesviral hematopoietic necrosis disease caused by cyprinid herpesvirus 2(CyHV-2)results in huge economic losses for the gibel carp(Carassius auratus gibelio)aquaculture industry.A vaccination strategy is a feasible method to prevent CyHV-2 infection and the resulting economic losses.In this study,inactivators(includingβ-propiolactone,formaldehyde and binary ethylenimine)were used to prepare an inactivated vaccine.The optimal inactivated CyHV-2 vaccine(0.2%β-propiolactone inactivates CyHV-2 for 48 h)mixed withβ-glucan,anisodamine,chitosan,and astragalus polysaccharide(APS)adjuvants were injected into gibel carp.Theβ-glucan and APS combined with inactivated vaccine significantly improved the relative immune protection rate of gibel carp against CyHV-2 infection.The highest specific antibody levels inβ-glucan and APS groups were found in serum by ELISA assay and antibody neutralization titer.Furthermore,adjuvant addition groups produced better results for lysozyme,total superoxide dismutase,and complement C3 in serum.β-glucan and APS combined with vaccine treatment effectively enhanced mRNA expressions of important immune genes(IL-1β,IgM,IL-2 and IFN-γ2).Minimal tissue lesions(gill,spleen,and head kidney)and virus replication were seen in theβ-glucan and APS groups by histopathological examination and expression analysis of CyHV-2 TK gene.These results indicated that the combination of inactivated vaccine and adjuvantβ-glucan or APS is an effective method against CyHV-2 infection and provided a case study reference for prevention of fish viral diseases using inactivated vaccines.
