Simulating leaf net CO_2 assimilation rate of C_3 & C_4 plants and its response to environmental factors

Basic structure and algorithm of leaf mechanism photosynthesis model were described in first part of this study based on former researcher results. Then, considering some environmental factors influencing on leaf photosynthesis, three numerical sensitivity experiments were carried out. We simulated the sing le leaf net CO2 assimilation, which acts as a function of different light, carbo n dioxide and temperature conditions. The relationships between leaf net photosy nthetic rate of C3 and C4 plant with CO2 concentration intercellular, leaf tempe rature, and photosynthetic active radiation (PAR) were presented, respectively. The results show the numerical experiment may indicate the main characteristic o f plant photosynthesis in C3 and C4 plant, and further can be used to integrate with the regional climate model and act as land surface process scheme, and bett er understand the interaction between vegetation and atmosphere.

Simulating leaf net CO2 assimilation rate of C3 & C4 plants and its response to environmental factors

Basic structure and algorithm of leaf mechanism photosynthesis model were described in first part of this study based on former researcher results. Then, considering some environmental factors influencing on leaf photosynthesis, three numerical sensitivity experiments were carried out. We simulated the sing le leaf net CO2 assimilation, which acts as a function of different light, carbo n dioxide and temperature conditions. The relationships between leaf net photosy nthetic rate of C3 and C4 plant with CO2 concentration intercellular, leaf tempe rature, and photosynthetic active radiation (PAR) were presented, respectively. The results show the numerical experiment may indicate the main characteristic o f plant photosynthesis in C3 and C4 plant, and further can be used to integrate with the regional climate model and act as land surface process scheme, and bett er understand the interaction between vegetation and atmosphere.

Prediction of Photosynthetic Carbon Assimilation Rate of Individual Rice Leaves under Changes in Light Environment Using BLSTM-Augmented LSTM

A model to predict photosynthetic carbon assimilation rate(A)with high accuracy is important for forecasting crop yield and productivity.Long short-term memory(LSTM),a neural network suitable for time-series data,enables prediction with high accuracy but requires mesophyll variables.In addition,for practical use,it is desirable to have a technique that can predict A from easily available information.In this study,we propose a BLSTM augmented LSTM(BALSTM)model,which utilizes bi-directional LSTM(BLSTM)to indirectly reproduce the mesophyll variables required for LSTM.The most significant feature of the proposed model is that its hybrid architecture uses only three relatively easy-to-collect external environmental variables—photosynthetic photon flux density(Q_(in)),ambient CO_(2) concentration(C_(a)),and temperature(T_(air))—to generate mesophyll CO_(2) concentration(C_(i))and stomatal conductance to water vapor(g_(sw))as intermediate outputs.Then,A is predicted by applying the obtained intermediate outputs to the learning model.Accordingly,in this study,1)BALSTM(Q_(in),C_(a),T_(air))had a significantly higher A prediction accuracy than LSTM(Q_(in),C_(a),T_(air))in case of using only Q_(in),C_(a),and T_(air);2)BALSTMC_(i),g_(sw),which had C_(i) and g_(sw) as intermediate products,had the highest A prediction accuracy compared with other candidates;and 3)for samples where LSTM(Q_(in),C_(a),T_(air))had poor prediction accuracy,BALSTMC_(i),g_(sw)(Q_(in),C_(a),T_(air))clearly improved the results.However,it was found that incorrect predictions may be formed when certain factors are not reflected in the data(e.g.,timing,cultivar,and growth stage)or when the training data distribution that accounts for these factors differs from the predicted data distribution.Therefore,a robust model should be constructed in the future to improve the prediction accuracy of A by conducting gasexchange measurements(including a wide range of external environmental values)and by increasing the number of training data samples.

Physiological Performances of Temperate Vegetables with Response to Chronic and Acute Heat Stress

In face of climate change catastrophes, understanding the thermal limits and optimal physiological thermal window food crop is of particular urgency. This research aims to evaluate: 1) how physiological performances of plant will change with increasing chronic and acute heat stress;2) if the examined parameters form a hierarchy in terms of thermal tolerance;and 3) the optimal thermal window and critical temperatures of the examined plants with response to chronic and acute heat stress. Six temperate vegetables were subjected to chronic and acute heat stress and a suite of physiological parameters were evaluated. Dose responses were observed in shoot fresh weight, photosynthetic gas exchange, photosynthetic oxygen evolution, electron transfer rate, photo- and non-photochemical quenching with significant drop in performance as early as 28°C for selected species. Conversely, ratio of variable to maximum fluorescence (Fv/Fm) was not affected by heat stress until 46°C in chronic heat stress. Examining the temperature at which a measured parameter’s performance dropped by 50% compared to control (LT50), a distinct hierarchy of the indices was observed for Canasta, recombinant inbred line 141, Lactuca serriola and Lactuca sativa (L. “Salinas”): shoot fresh weight, representing the highest integrated level of photosynthesis was the most sensitive to thermal stress (28°C - 30°C), followed by oxygen evolution (35°C - 45°C) while non-photochemical and photochemical quenching which is subcellular function of stress alleviation had a much higher capacity failure temperature (47°C - 60°C). It is expected that Fv/Fm ratio, a measurement of sub-cellular structural integrity, will approach that of non-photochemical and photochemical quenching, if not exceeding it. By examining the photosynthetic parameters via their hierarchy of biological organization, it can be inferred that plants like Arugula and recombinant inbred line 192 are already operating near their thermal limit and have less energetic investment into heat stress mediation whereas L. serriola prioritizes thermal tolerance at the expense of photosynthesis efficiency.

Analysis on Dry Matter Production Characteristics of Super Hybrid Rice

Six middle-season indica hybrid rice combinations, including five super hybrid rice combinations with the high yield about 10.5 t/ha and a check hybrid rice combination Shanyou 63 with a yield potential about 9.5 t/ha, were used as materials to study the dry matter production characteristics. The super hybrid rice showed a high ability in dry matter production and accumulation and its yield enhanced with the increase of dry matter accumulation. The advantage period of dry matter production in the super hybrid rice was mainly at the middle and late growth stages compared with the check. The grain yield had no significant correlation with the dry matter accumulation before the elongation stage while had a significantly positive correlation with the dry matter accumulation from the elongation to maturity stages in super hybrid rice. There were more dry matter in vegetative organs at the heading stage in the super hybrid rice but its contribution to yield （apparent conversion percentage） was averagely 4.3 percent points lower than that in the check. For crop growth rate （CGR）, the comparative advantage of super hybrid rice was at the middle and late stages, especially after flowering. Moreover, as the rising of leaf area index （LAI） and leaf area duration （LAD）, CGR enhanced. The total LAD and the mean of lAD per day of super hybrid rice was about 14.79% and 10.31% higher than those of the check, respectively. The results indicate that the high yield of super hybrid rice mostly comes from the products of photosynthesis after heading, which is shown by the increased CGR at middle and later stages. It is suggested that LAD character might be used to better explain the advantage in the dry matter production of super hybrid rice than LAI.

Timing of N Application Affects Net Primary Production of Soybean with Different Planting Densities

Understanding the relationship between the timing of N fertilizer applications and crop primary production is crucial for achieving high yield and N use efifciency in agriculture. This study investigated the effects of starting-N plus topdressing N applications （as compared to the common practice of all basal application） on soybean photosynthetic capacity under different planting densities. A ifeld experiment was conducted in two growing seasons （2011 and 2012）, and the soybean （Glycine max L. Merrill） cultivar was Dongnong 52, three planting densities （20, 25 and 30 plants m-2）, and four N fertilizer application patterns （all N fertilizer of 6 g N m-2 as basal fertilizer, all N fertilizer as topdressing at beginning pod stage （R3）, 1.8 g N m-2 as basal fertilizer and 4.2 g N m-2 as topdressing at stage R3 and full pod stage （R4）, respectively）. The results indicated that under the same planting density, compared to applying all N as basal fertilizer, the application of starter-N plus topdressing N substantially reduced the rate of pod abscission, and enhanced leaf area index （LAI） signiifcantly at beginning seed stage （R5） （P〈0.05）, net assimilation rate （NAR） during stages R4-full seed stage （R6） （P〈0.05）, contribution rate of post-seed iflling assimilate to seed （CPA） （P〈0.05）, and yield （P〈0.05）. Applying topdressing N at stage R4 resulted in higher net primary production and yield than applying topdressing N at stage R3. When applying starter-N plus topdressing N at planting density of 25 plants m-2, LAI after stage R5 and NAR after stage R4 were increased by 5.92-16.3%（P〈0.05） and 13.7-26.6%（P〈0.05） with the planting density of 20 plants m-2, respectively, and yield was 8.46-14.0%（P〈0.05） higher than that under 20 plants m-2. When planting density increased to 30 plants m-2, only LAI during stages R4-R5 and NAR during stages R4-R5 increased by applying starter-N plus topdressing N, while the other indexes declined. Overall, results of this study demonstrated that applying starter-N plus topdressing N could signiifcantly enhance soybean photosynthetic capacity after stage R5 at planting density of 25 plants m-2.

Growth and yield of rice as affected by transplanting dates and seedlings per hill under high temperature of Dera Ismail Khan, Pakistan

Studies were initiated for two consecutive years to find out the effect of time of transplanting and seedlings hill^-l on the productivity of rice in Dera Ismail Khan district of North West Frontier Province （NWFP）, Pakistan. The experiment was laid out in a randomized complete block design with split plot arrangements. Main plots consisted of four transplanting dates viz. 20th and 27th of June and 4th and 1 lth of July while sub-plots contained 1, 2, 3 or 4 seedlings hill^-1. Among transplanting dates, June 20th planted crop gave highest paddy yield and net return with I seedling hill^-1. It explains that the use of more seedlings hill^-1 not only adds to cost but is also a mere wastage of natural resources. Based on research findings, we conclude that the use of I seedling hill^-1 is most appropriate for timely sowing otherwise 4 seedlings hill^-1 should be used to compensate for the yield gap in late transplanted rice.

Study on intermittent irrigation for Paddy Rice: Ⅱ. Crop Responses

Effect of intermittent irrigation on the production of paddy rice was studied in a well-puddled paddy field with four treatments and 2 replicates: continuous flooding irrigation (CFI), and intermittent irrigation Ⅱ-O, Ⅱ-1 and Ⅱ-2, in which plants were re-irrigated when the soil water potential fell below 0, -10, and -20 kPa, respectively, at soil depth of about 5 cm. Results showed that the reduction in soil water potential to about -10 or -20 kPa did not significantly affect the number of grains and the percentage of ripened grains. While, a lower crop growth rate (CGR) resulted from a decrease in the net assimilation rate (NAR) during intermittent irrigation Ⅱ-1 and Ⅱ-2, and there was also a reduction in the leaf area index (LAI) during intermittent irrigation Ⅱ-2. Senescence of lower leaves on stems was promoted in treatments Ⅱ-1 and Ⅱ-2 at the ripening stage. Early senescence at ripening stage and water stress around midday decreased the rate of photosynthesis in leaves, causing the lower NAR. These physiological responses of the plants were responsible for the reduction in the dry matter production and grain yield in the intermittent irrigation treatments.

Effect of Establishment Methods and Weed Management Practices on Some Growth Attributes of Rice

Studies were carried out for two years to evaluate the effect of methods of sowing and weed control practices on the productivity of transplanted and direct wet-seeded rice in Dera Ismail Khan, NWFP, Pakistan. The experiment was laid out in a randomized complete block design with a split plot arrangement. The planting techniques viz. transplanting and direct seeding were maintained in main plots while weed control practices included the use of granular herbicide Sunstar 15WG （ethoxy sulfuron）, Machete 60EC （butachlor）, conventional hand weeding, and the weedy check （control） were assigned to the sub-plots. Data were recorded on weed parameters like weed density and dry weed biomass 60 and 90 days after sowing （DAS）; agronomic parameters including plant population, number of panicles and paddy yield and physiological parameters like leaf area index and net assimilation rate 45 and 90 DAS. The planting methods and weed management significantly influenced most of the parameters studied. The data revealed that the paddy yield and its components were significantly higher in the transplanted method than that in direct-seeded method, while the weed density and biomass were lower in the transplanted plots than the direct-seeded plots. Among weed management tools, the maximum paddy yield was obtained in hand weeding, closely followed by herbicide application Machete 60EC during both cropping seasons.

Alterations in Growth and Yield of Camelina Induced by Different Planting Densities under Water Deficit Stress

Camelina(Camelina sativa L.)is famous for its oil quality and unique fatty acid pattern.Growth and yield of crops reduced under water deficit conditions.Environmental threat such as drought or water deficit condition is the emerging problem which creates the negative impact on the growth of plants.Based upon the current situation a pot study was performed in rain out-shelter to explore the effect of different plant densities(15,10 and 5 plants per pot)on growth and seed yield of two camelina genotypes under normal(100%WHC)and water deficit(60%WHC)conditions by using completely randomized design with factorial arrangement having three replicates.Results indicated that individual effects of plant densities and water deficit stress levels considerably influenced the growth and seed yield of camelina but interaction effects did not indicate any significant variation.Maximum values of leaf area index(LAI)and crop growth rate(CGR)were recorded in P_(3) treatment(15 plants per pot).However,maximum values of leaf area duration(LAD),net assimilation rate(NAR),yield and yield components were observed in the treatment P_(1)(5 plants per pot).Water deficit condition(60%WHC)significantly minimized the growth,seed yield(0.82 g/m^(2))and yield components of camelina genotypes.Both camelina genotypes(611 and 618)did not differ significantly under water deficit conditions.

Short-term responses to salinity of seabuckthorn (Hippophae rhamnoides L.) seedlings in the extremely cold and saline Qinghai region of China

The investigation of the mechanisms of plant adaptation to stressor action is one of the leading directions of current biological studies. To understand the mechanism of salt tolerance of seabuckthom （Hippophae rhamnoides L.） and identify its ability to cope with the salinity effect in the saline and extremely cold region of Qinghai, China, a test was conducted with two-year-old seedlings subjected to 0, 200, 400 and 600 mmol·L^-1 NaClsolutions for 30 d. The results show that with an increase in salinity, the biomass of H. rhamnoides seedlings clearly decreased. Leaf water potential （ψw） and relative water content （RWC） were significantly reduced under salinity, with severe water shortages appearing in leaves. At the same time, the total chlorophyll content declined markedly. When salinity increased and stress time prolonged, the net CO2 assimilation rate （A） significantly declined. Intercellular CO2 concentration （Ci） declined at first and was then followed by an increase over the stress time. We conclude that H. rhamnoides grown in the extremely cold and saline region of Qinghai has a certain resistance to salt, which can be planted at appropriate salinity levels.

Effects of Root-Zone Temperature on Photosynthesis, Productivity and Nutritional Quality of Aeroponically Grown Salad Rocket (Eruca sativa) Vegetable

Although tropical high ambient temperature and humidity severely reduced the productivity of temperate plants, temperate vegetable crops such as lettuce have been successfully grown in Singapore by only cooling its root-zone. In this paper, a cool Meditteranean vegetable, Eruca sativa, was studied to understand how different RZTs can impact its shoot productivity, photosynthesis and nutritional quality. All plants were cultivated using aeroponic systems in a tropical greenhouse under hot ambient conditions where roots were subjected to four different root-zone temperatures (RZTs) of 20°C-RZT, 25°C-RZT, 30°C-RZT and fluctuating ambient temperatures ranged from 25°C to 38°C [25°C/38°C (ambient)]-RZT. Parameters studied include shoot fresh weight (FW), photosynthetic gas exchange, midday chlorophyll (Chl) fluorescence Fv/Fm ratio, Chl fluorescence photochemical quenching (qP), non-photochemical quenching (qN) and electron transport rate (ETR), total phenolic compounds and mineral content such as potassium (K), calcium (Ca), magnesium (Mg) and iron (Fe). Among the 4 different RZT treatments, E. sativa plants grown under ambient-RZT (25/38°C-RZT) had the lowest shoot and root FW while those plants grown under 20°C-RZT had highest productivity of shoot and root. However, there were no significant differences in shoot and root FW in plants grown at 25°C- and 30°C-RZT. Compared to plants grown under 25°C/38°C (ambient-RZT), light-saturated photosynthetic CO2 assimilation rate (Asat) and stomatal conductance (gssat) were similarly higher in 20°C-, 25°C- and 30°C-RZT. All plants had midday Chl fluorescence Fv/Fm ratio lower than <0.8 ranged from 0.785 to 0.606 with the highest and lowest ratios recorded in 20°C-RZT and ambient-RZT plants, respectively. These results indicate that cooling the RZ of E. sativa plants protected their PS II from photoinactivation during midday in the greenhouse. There were no significant differences observed in photochemical quenching (qP), non-photochemical quenching (qN) and electron transport rate among plants grown under 20°C-, 25°C- and 30°C-RZT. However, plants grown under ambient-RZT had lower qP, qN and ETR compared to all other plants. E. sativa at 20°C-RZT with the best developed roots had the highest dietary mineral (K, Mg, Ca and Fe) contents but lower total phenolics content. In contrast, ambient-RZT, plants with poorly developed roots had the lowest mineral content but highest total phenolic content. The results of this study suggest that cooling of roots is a feasible method for the cultivation of E. sativa in the tropic, which enhances the content of dietary minerals in shoots.

Carbon Dioxide Concentrations and Light Levels on Growth and Mineral Nutrition of Juvenile Cacao Genotypes

In many countries cacao (Theobroma cacao L.) is invariably grown as an understory crop in agroforestry types of cropping systems and subjected to low levels photosynthetic photon flux density (PPFD) due to presence of large number of upper story shade trees with poorly managed canopy structure. In recent years carbon dioxide concentration in the atmosphere is steadily increasing and it is unclear what impact this will have on performance of cacao grown under shade of upper story shade trees. A climatically controlled greenhouse experiment was undertaken to evaluate the effects of ambient and elevated carbon dioxide (400 and 700 μmol·mol-1) and three levels of PPFD (100, 200, and 400 μmol·m-2·s-1) on growth, and macro- and micronutrient use efficiency of three genetically contrasting cacao genotypes (CCN 51, VB 1117 and NO 81). Intraspecific variations were observed in cacao genotypes for growth parameters at ambient to elevated carbon dioxide and low to adequate levels of PPFD. With the exceptions of total root length and leaf area, irrespective of carbon dioxide and PPFD levels, all three genotypes showed significant differences in all the growth parameters. For all the cacao genotypes, increasing PPFD from 100 to 400 μmol·m-2·s-1 and carbon dioxide from 400 to 700 μmol·mol-1 increased overall growth parameters such as leaf, shoot and root biomass accumulation, stem height, leaf area, relative growth rate and net assimilation rate. Irrespective of carbon dioxide and PPFD, invariably genotypes differed significantly in macro-micronutrient uptake parameters such as concentration, uptake, influx, transport and use efficiency. With few exceptions, raising PPFD from 100 to 400 μmol·m-2·s-1 and carbon dioxide from 400 to 700 μmol·mol-1 increased nutrient use efficiency for all the cacao genotypes. Elevated carbon dioxide and adequate PPFD are beneficial in improving cacao growth and mineral nutrient uptake and use efficiency.

Photosynthetic characteristics of intercropped winter wheat under limited supplemental irrigation in the semiarid northwestern China

A field experiment was conducted for intercropped winter wheat （Triticum aestivum） in 2002/2003 to evaluate the effects of limited supplemental irrigation on photosynthetic characteristics of intercropped winter wheat in semiarid environment. The result indicated that significances occurred in grain yield between the intercropped wheat treatments and sole wheat control （CKW）, and in yield between the irrigated intercropped wheat plots （WC2W, WC3W, WC5W） and not irrigated （WC1W） except for WC4W plots with nearly the same yield as WC1W. In comparison with CKW, 11.8%, 18.5%, 23.6%, 11.5%, and 30.7% of yield increase in the intercropped wheat plots were obtained in WCIW, WC2W, WC3W, WC4W, and WC5W respectively. Compared to the intercropped wheat plots without irrigation, yields in WC2W, WC3W, and WC5W were improved by 5.9%, 10.5%, and 16.9%, respectively. The dynamics of CGR and NAR in both intercropped （WC1W-WC5W） and solely cropped wheat （CKW） showed a type of ＂single peak＂ curves, with both the maximum CGR and NAR occurred during jointing to heading （14/4-6/5） of wheat. In addition, soil water potential （SWP） fluctuated as a function of the precipitation and limited supplemental irrigation.

Nitrogen Fertilization on Maize Sowing:Plant Growth and Seed Vigor

The management of fertilization in maize plants is a preponderant factor in the search for higher yields and obtaining quality seeds. In this sense, the study of nitrogen (N) management is predominant in improving the efficiency of its application. The objective of this study was to evaluate the growth, partition assimilation and vigor expression of maize seeds submitted at different doses of nitrogen applied at sowing. The treatments were constituted by different doses of nitrogen fertilization at sowing, being: 25%, 50%, 75% and 100% of the recommended dose for the crop, according to the recommendation of nitrogen fertilization. The plants were collected at regular intervals of 21 days after sowing until the end of the growth cycle. For the analysis of growth and partition of dry matter between the organs, the accumulation of dry matter and leaf area was measured. The experimental design was randomized blocks in a 4 × 7 factorial scheme (four N rates at sowing and seven collection times with three replications). The maize plants under the influence of nitrogen fertilization at sowing presented differential growth and distinct responses in the assimilated partition between their structures, with the intensity dependent on the dose used. Plants grown using 75% of the recommended dose of N at sowing had a higher relative growth rate, with more pronounced effects compared to the other doses. The expression of maize seed vigor increases to an increase of 82.5% N at sowing.

comSeedling growth of five tropical dry forest tree species in relation to light and nitrogen gradients

Aims Increasing anthropogenic nitrogen(N)deposition has been claimed to induce changes in species composition and community dynamics.A greenhouse experiment was conducted to examine the effect of increased N availability on growth and functional attributes of seedlings of five tree species with different life history characteristics under varying irradiances.The following questions have been addressed:(i)how do the pioneer and non-pioneer species respond in absolute growth and relative growth rate(RGR)to the interaction of light and nitrogen?(ii)how does the interaction between irradiance and nitrogen availability modulate growth attributes(i.e.functional attributes)?(iii)is there any variation in growth responses between leguminous and non-leguminous species along the light and nitrogen gradients?Methods Seedlings of five tree species(Acacia catechu,Bridelia retusa,Dalbergia sissoo,Lagerstroemia parviflora and Terminalia arjuna)were subjected to twelve combinations of irradiance and N levels.Various growth traits,including height(HT),basal area(BA),whole plant dry biomass(MD),leaf mass per unit area(LMA),leaf area ratio(LAR),net assimilation rate(NAR),RGR,biomass fractions,root-toshoot ratio(R:S)and leaf nitrogen content,were studied to analyse intra-and inter-specific responses to interacting light and N gradients.Important Findings Significant interactions for irradiance and N availability for majority of growth attributes indicates that growth and biomass allocation of seedlings were more responsive to N availability under high irradiance.However,species responded differentially to N addition and they did not follow successional status.Slow growers(B.retusa,a shade-tolerant species and L.parviflora,a light demander)exhibited greater response to N enrichment than the fast growers(A.catechu,D.sissoo and T.arjuna).However,N-mediated increment in growth traits was greater in non-legumes(B.retusa,L.parviflora and T.arjuna)compared with that of legumes(A.catechu and D.sissoo).Allocation of biomass to root was strongly suppressed at the highest N supply across species;however,at high irradiance and high N availability,a greater suppression in R:S ratio was observed for B.retusa.NAR was a stronger determinant of RGR relative to LAR,suggesting its prominent role in increased RGR along increasing irradiances.Overall,a higher growth response of slow-growing species to elevated N levels,particularly the non-pioneers(B.retusa and L.parviflora)suggests that future N deposition may lead to perturbations in competition hierarchies and species composition,ultimately affecting community dynamics in nutrient-poor tropical dry forests.