Objective： To investigate the immune regulation of flavonoid of Astragalus membranaceus's stem and leaf(FAM-sl). Methods： Changes of total T cell count and subsets in mice were determined by monoclonal antibody ...Objective： To investigate the immune regulation of flavonoid of Astragalus membranaceus's stem and leaf(FAM-sl). Methods： Changes of total T cell count and subsets in mice were determined by monoclonal antibody assay before and after treatment with FAM-sl, and the lymphokine activated killer cell (LAK) activity was tested simultaneously by isotope label method.Results： FAM-sl could promote the proliferation of lymphocytes induced by ConA, raise the total T cell count and regulate the T cell subsets disturbance, and elevate the LAK activity induced by recombinant interleukin-2 (rIL-2).Conclusion： FAM-sl possesses effects of immune stimulation and immune regulation in treating immunosuppressive mice. This study provides experimental evidence for clinical application of FAM-sl.展开更多
Objective: To investigate the immune regulation of flavonoid of Astragalus membranaceus′ s stem and leaves (FAM). Methods: Changes of total T cell count and subsets in mice were determined by monoclonal antibody a...Objective: To investigate the immune regulation of flavonoid of Astragalus membranaceus′ s stem and leaves (FAM). Methods: Changes of total T cell count and subsets in mice were determined by monoclonal antibody assay before and after treatment with FAM, and the lymphokine activated killer(LAK) activity was tested simultaneously by isotope technique. Results: FAM could promote the proliferation of lymphocytes induced by ConA, raise the T cell count and regulate the T cell subsets disorder, elevate the LAK activity induced by IL 2. Conclusion: FAM possesses the effect of immune stimulation and immune regulation in treating immunosuppressive mice. This study provides the experimental basis for clinical application of FAM.展开更多
文摘Objective： To investigate the immune regulation of flavonoid of Astragalus membranaceus's stem and leaf(FAM-sl). Methods： Changes of total T cell count and subsets in mice were determined by monoclonal antibody assay before and after treatment with FAM-sl, and the lymphokine activated killer cell (LAK) activity was tested simultaneously by isotope label method.Results： FAM-sl could promote the proliferation of lymphocytes induced by ConA, raise the total T cell count and regulate the T cell subsets disturbance, and elevate the LAK activity induced by recombinant interleukin-2 (rIL-2).Conclusion： FAM-sl possesses effects of immune stimulation and immune regulation in treating immunosuppressive mice. This study provides experimental evidence for clinical application of FAM-sl.
文摘Objective: To investigate the immune regulation of flavonoid of Astragalus membranaceus′ s stem and leaves (FAM). Methods: Changes of total T cell count and subsets in mice were determined by monoclonal antibody assay before and after treatment with FAM, and the lymphokine activated killer(LAK) activity was tested simultaneously by isotope technique. Results: FAM could promote the proliferation of lymphocytes induced by ConA, raise the T cell count and regulate the T cell subsets disorder, elevate the LAK activity induced by IL 2. Conclusion: FAM possesses the effect of immune stimulation and immune regulation in treating immunosuppressive mice. This study provides the experimental basis for clinical application of FAM.
