AIM To investigate the structural and functional characte-ristics of palmar hypodermal tissue vascularization in Dupuytren's contracture patients of different agegroups.METHODS Eighty-seven Dupuytren's contrac...AIM To investigate the structural and functional characte-ristics of palmar hypodermal tissue vascularization in Dupuytren's contracture patients of different agegroups.METHODS Eighty-seven Dupuytren's contracture patients underwent partial fasciectomy. Twenty-two of them were less than 55 years old(Y-group, n = 22); the others were 55 and older(O-group, n = 65). In surgically excised representative tissue samples, a histomorphometric analysis of the perforating arteries of the palmar aponeurosis and stereologic analysis of hypodermis vascularity were performed. The method of laser flowmetry estimated the microcirculation of the skin of the palm.RESULTS Frequency of cases with rapid development of contracture(less than 5 years) was 13.6% in the Y-group and 40% in the O-group, P < 0.05. The external and luminal diameters of perforating arteries in palmar fascia were decreased more severely in Y. The thickness of intima increased three times compared with healthy control, and the intima/media relation also increased, especially in O. Increased numerical and volumetric micro-vessel densities in hypodermis, percentage of large vessels(more than 12 μm in diameter), and percentage of vessels with signs of periadventitial inflammatory infiltration were noted in Y. The percentage of vessels with adventitial fibrosis was greater in O than in Y. Base capillary flow in Y was increased compared to healthy control subjects and to O, and peak capillary flow was increased in comparison with control.CONCLUSION Compared to the O-group, Y-group patients exhibited more severe constrictive remodeling of palmar fascia perforating arteries supplying hypodermis but more expressed compensatory changes of its capillarization.展开更多
目的研究四肢发生制动性挛缩后,深筋膜组织内Ⅰ型胶原表达的变化以及肢体不易被拉伸的原因。方法将兔的右侧股骨干造成重叠短缩型骨折作为实验组,未作处理的左后肢作为对照组。1个月和2个月后分别切取双后肢股部外侧中段深筋膜,利用天...目的研究四肢发生制动性挛缩后,深筋膜组织内Ⅰ型胶原表达的变化以及肢体不易被拉伸的原因。方法将兔的右侧股骨干造成重叠短缩型骨折作为实验组,未作处理的左后肢作为对照组。1个月和2个月后分别切取双后肢股部外侧中段深筋膜,利用天狼猩红染色、免疫组化染色来观察含有Ⅰ型胶原的纤维束的显微形态,采用Western blot法检测Ⅰ型胶原的表达、实时荧光定量PCR法测定Ⅰ型胶原相关基因的表达的情况。结果实验组骨折后1个月和2个月的Ⅰ型胶原纤维长度均明显小于对照组[(58.3±18.5)μm vs (81.7±13.4)μm,(51.1±16.2)μm vs (83.5±14.6)μm,P<0.01]。与对照组比较,实验组骨折后1个月和2个月的Ⅰ型胶原表达量明显减少(P<0.05),Ⅰ型胶原蛋白m RNA的相对表达量明显下降(P<0.05)。结论肢体挛缩后深筋膜内Ⅰ型胶原含量降低并导致肢体软组织延展性下降。展开更多
基金Supported by The RF Ministry of Health within Government Mandated Program for FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"(RISC"RTO") for Scientific Research in 2018-2020 Yr./Yrs.No.АААА-А18-118011190119-5 from 11/01/2018
文摘AIM To investigate the structural and functional characte-ristics of palmar hypodermal tissue vascularization in Dupuytren's contracture patients of different agegroups.METHODS Eighty-seven Dupuytren's contracture patients underwent partial fasciectomy. Twenty-two of them were less than 55 years old(Y-group, n = 22); the others were 55 and older(O-group, n = 65). In surgically excised representative tissue samples, a histomorphometric analysis of the perforating arteries of the palmar aponeurosis and stereologic analysis of hypodermis vascularity were performed. The method of laser flowmetry estimated the microcirculation of the skin of the palm.RESULTS Frequency of cases with rapid development of contracture(less than 5 years) was 13.6% in the Y-group and 40% in the O-group, P < 0.05. The external and luminal diameters of perforating arteries in palmar fascia were decreased more severely in Y. The thickness of intima increased three times compared with healthy control, and the intima/media relation also increased, especially in O. Increased numerical and volumetric micro-vessel densities in hypodermis, percentage of large vessels(more than 12 μm in diameter), and percentage of vessels with signs of periadventitial inflammatory infiltration were noted in Y. The percentage of vessels with adventitial fibrosis was greater in O than in Y. Base capillary flow in Y was increased compared to healthy control subjects and to O, and peak capillary flow was increased in comparison with control.CONCLUSION Compared to the O-group, Y-group patients exhibited more severe constrictive remodeling of palmar fascia perforating arteries supplying hypodermis but more expressed compensatory changes of its capillarization.
文摘目的研究四肢发生制动性挛缩后,深筋膜组织内Ⅰ型胶原表达的变化以及肢体不易被拉伸的原因。方法将兔的右侧股骨干造成重叠短缩型骨折作为实验组,未作处理的左后肢作为对照组。1个月和2个月后分别切取双后肢股部外侧中段深筋膜,利用天狼猩红染色、免疫组化染色来观察含有Ⅰ型胶原的纤维束的显微形态,采用Western blot法检测Ⅰ型胶原的表达、实时荧光定量PCR法测定Ⅰ型胶原相关基因的表达的情况。结果实验组骨折后1个月和2个月的Ⅰ型胶原纤维长度均明显小于对照组[(58.3±18.5)μm vs (81.7±13.4)μm,(51.1±16.2)μm vs (83.5±14.6)μm,P<0.01]。与对照组比较,实验组骨折后1个月和2个月的Ⅰ型胶原表达量明显减少(P<0.05),Ⅰ型胶原蛋白m RNA的相对表达量明显下降(P<0.05)。结论肢体挛缩后深筋膜内Ⅰ型胶原含量降低并导致肢体软组织延展性下降。
