Studies on the Attached Cultivation of Filamentous Oleaginous Microalga Tribonema minus

Attached cultivation is a promising method for microalgal biomass production. Filamentous oleaginous microalga Tribonema minus(hereafter T. minus) has shown a remarkable potential for biofuel production in terms of its high lipid content. However, the strain has only been cultivated in suspended cultivation systems including open pond and closed photobioreactors. Here, we attempted to study the attached cultivation of T. minus, which might be helpful for its scale-up cultivation and industrial applications. As the results, the optimal conditions for T. minus growth in the attached biofilm are 200 μmol photons m^-2 s^-1 of light intensity and 5% of CO2, and the maximum biomass density of 223 g m^-2 has been achieved under the light intensity. The non-woven fabric as substratum was found as the best substratum in thin layer attached bioreactor, on which the average biomass productivity of T. minus is about(9.73 ± 2.19) g m^-2)d^-1. Furthermore, two attached bioreactor systems, rotary drum and rotation disc, were designed following the light dilution strategy and introduced into T. minus cultivation. The highest footprint areal biomass productivity of these two systems is 33 and 47.1 g m^-2 d^-1, respectively, much higher than that in suspended cultivation system. The results shows that T. minus can be cultured with attached cultivation method to improve its biomass productivity.

Mechanism of Tourists’Happiness in Traditional Village Tourism: A Perspective Grounded in Nostalgia and Cultural Attachment

In recent years,traditional villages have emerged as popular tourist destinations due to their distinctive resource value.Typically situated in remote and less developed areas,traditional villages boast primitive natural surroundings and rich historical and cultural heritage.These characteristics offer urban dwellers a temporary escape from the complexities of modern life,allowing them to experience physical and mental rejuvenation,mental relaxation,and overall happiness during their visit.By conducting a review of the existing literature concerning tourists’happiness in traditional villages,this study establishes a framework to understand tourists’happiness in traditional village tourism.Using the Qiangang ancient village in Conghua District,Guangzhou as a case study,the research examines the current state of rural characteristics,the establishment of cultural brands,and the promotion of the active utilization of ancient villages within the context of integrating culture,sports,tourism,and agriculture.Based on this analysis,the author proposes strategies to enhance tourists’happiness,including the preservation and development of rural characteristics,the promotion and preservation of local cultural attributes,and the collaborative advancement of culture,sports,tourism,and agriculture to engage key stakeholders actively.