Effect of culture methods on individual variation in the growth of sea cucumber Apostichopus japonicus within a cohort and family

There is substantial individual variation in the growth rates of sea cucumber Apostiehopus japonicus individuals. This necessitates additional work to grade the seed stock and lengthens the production period. We evaluated the influence of three culture methods （free-mixed, isolated-mixed, isolated-alone） on individual variation in growth and assessed the relationship between feeding, energy conversion efficiency, and individual growth variation in individually cultured sea cucumbers. Of the different culture methods, animals grew best when reared in the isolated-mixed treatment （i.e., size classes were held separately）, though there was no difference in individual variation in growth between rearing treatment groups. The individual variation in growth was primarily attributed to genetic factors. The difference in food conversion efficiency caused by genetic differences among individuals was thought to be the origin of the variance. The level of individual growth variation may be altered by interactions among individuals and environmental heterogeneity. Our results suggest that, in addition to traditional seed grading, design of a new kind of substrate that changes the spatial distribution of sea cucumbers would effectively enhance growth and reduce individual variation in growth of sea cucumbers in culture.

The Effect of Three Culture Methods on Intensive Culture System of Pacific White Shrimp (Litopenaeus vannamei)

Different culture methods may affect the intensive culture system of Pacific white shrimp(Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84 d.The three methods involved shrimp cultivation in different tanks,i.e.,outdoor tanks with cement bottom(mode-C),greenhouse tanks with cement bottom(mode-G) and outdoor tanks with mud-substrate(mode-M).Results showed that water temperature was significantly higher in mode-G than that in mode-C(P < 0.05).In contrast to the other two treatments,mode-M had stable pH after 50 d cultivation of shrimps.In the mid-late period,the average concentrations of TAN,NO2-N,DIP and COD were significantly lower in mode-M and mode-G compared with those in mode-C(P < 0.05).Despite lack of differences in the final shrimp weight among different treatments(P > 0.05),mode-M had significantly higher shrimp yield,survival rate and feed conversion rate(P < 0.05) than other modes.There were significant differences in revenue and net return among different treatments(P < 0.05).These demonstrated that the treatments of mode-G and mode-M were conductive to the intensive culture system of L.vannamei.

Study and promotion of safety culture using mixed methods research

Objective:With a positive safety culture,institutions offer the best quality and safe care to their patients.The objective of this study was to analyze patient safety culture from the perspective of the multidisciplinary team,to identify factors that influence patient safety culture,and to create/promote-jointly with the study participants-strategies for improving processes of change.Methods:The study design represented a mixed methods research approach,with a sequential explanatory design.A multidisciplinary team of workers at a general hospital was eligible for the study.To collect quantitative data,we administered the Safety Attitudes Questionnaire(SAQ).The qualitative phase was accomplished via focus groups(FGs),with participants from the first phase of the study using the principles of deliberative dialogue(DD)as a knowledge-translation strategy.The STROBE guideline was used to develop the study.Results:The overall SAQ score was positive(75.1±10.4).Negative scores were found in the fields of Safety Climate,Working Conditions,and Stress Recognition.Focus group discussions identified the aspects that create a negative impact on safety culture,such as ineffective communication,punitive approach in the event of errors,the lack of commitment and adherence to the protocols,and the non-recognition of the stress and the mistakes.Actions for the promotion of safety culture were developed and implemented during the study.Conclusions:The use of the principles of DD as a strategy for knowledge translation(KT)made it possible to identify and plan for joint actions to generate improvements in safety culture.

On C-E Translation Strategies and Methods of Zhiba Culture for Global Communication

Zhiba culture is a profound culture with a long history,but there are still many problems in the C-E translation of it,such as low popularity,few recipients,and so on,which hinder its communication and development.Therefore,it’s necessary to improve the quality of C-E translation of Zhiba culture.Based on the perspective of communication studies,combining with the principle of“Information First”,the principle of“The Audience First”,the paper explores the existing problems of Zhiba culture C-E translation and hopes to help to improve the present situation,to truly show the charm of Zhiba culture,to make it better accepted by the international community and optimize the global communication effect.

On Translation Methods of Culture Compensation

In the various methods of English Chinese translation,cultural differences will cause the loss of information,which willgreatly affect the quality of translation.Therefore,in order to express the meaning accurately,the translator must have a comprehen-sive understanding of the two cultures.The purpose of this paper is to help translators effectively reduce the loss of information,ex-press the original meaning completely and accurately,and improve the quality of translation.

Analysis of Landscape Vitality of Historical and Cultural Blocks Based on AHPFuzzy Comprehensive Evaluation Method:A Case Study of Daopashi Street in Anqing City

Historical and cultural blocks are witnesses of history and inheritors of culture. As one of the main spaces for outdoor interaction in historical and cultural blocks, the improvement of its vitality is of great significance for the improvement of residential environment and the better inheritance of history and culture. Taking Daopashi Street in Anqing City as an example, an evaluation model of landscape spatial vitality of historical and cultural blocks was constructed from three aspects of viewing function, store status and service facilities, and analytic hierarchy process was used to determine the index weight and vaguely evaluate the landscape spatial vitality of historical and cultural blocks. The results show that through the comparison of weight, architectural style(0.317), the practicability of service facilities(0.168) and plant landscape(0.165) had a significant impact on the landscape spatial vitality of historical and cultural blocks,and the landscape spatial vitality of historical and cultural blocks in Daopashi Street in Anqing City was at a good level.

Effects of nitrogen specification and culture method on growth of Enteromorpha prolifera

Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO3-N concentration was consistent with that estimated using the Monod equation （R2=0.9713, P〈0.01）. In the N O3-N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 μmol/L. Our results show that E. prolijkra can effectively utilize NH4^＋-N, NO3^-N, and NO^2-N and urea-N in the range of 5 to 50 μmol/L. NH4^＋-N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth.

Research on Protection Methods of Historic and Cultural Villages in Zhejiang Province

By the investigation of a few historical and cultural villages in Zhejiang Province,some problems during the process of protecting these villages were found,like similar construction pattern,coarse quality of construction,etc. which caused that villages were losing their characters. For this situation,the protection method of historical cultural villages was put forward,which was that to connect excavating character of different village with maintaining spatial fabrics of historical villages,and that different classes of building should have corresponding protection approach. Some advices were proposed for repairing and promoting historic and cultural elements,natural environment,and constructing infrastructure in the village,which will provide a reference for the construction of other historical and cultural villages.

Study on Surface Sterilization Method for Tissue Culture of Haloxylon ammodendron(C.A.Mey.) Bunge Seeds

[ Objective ] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron （ C. A. Mey. ）. [Method] H. ammodendron （C. A. Mey. ） seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings. [ Result] Sterilization of H. ammodendron （ C.A. Mey. ） seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min. Seeds with diameter φ 〉 2.0 mm were used as explants and achieved relatively high surviv- al rate of aseptic seedlings, which reached above 65%. [ Conclusion] This study established a surface sterilization method for tissue culture of H. ammodendron （ C. A. Mey. ） Bunge seeds.

On the Application of “Red Culture” in Urban Parks

In recent years, with the support of national policies and the people’s demand for cultural life, a wave of red tourism has been in full swing throughout the country. The revolutionary martyrs opened up new heavens and earth with their flesh and blood, and the great spirit of the Red Revolution carried forward the precious material and spiritual wealth of our socialist country. As the successor of socialism in the new era, inheriting the great spirit of the Chinese revolution and praising the red culture are important tasks for our contemporary people. Therefore, in the development and construction of urban parks, the red cultural connotation of urban parks should be given, which plays a very important role in improving the quality of the city and enhancing the charm of the city.

Design Method of Cultural Derivative Products of Museums from the Perspective of Regional Culture

To perfect innovative design of cultural derivative products of museums,promote the dissemination of museum collection culture,and enhance product connotation and cultural value,based on the research on regional culture,the relationship between regional culture and cultural derivative products of museums is analyzed,and design method of cultural derivative products of museums is summarized.

A Study of Educational Change as Culture Change--a Comparison of the Grammar-Translation Method and Communicative Language Teaching Method

The world is complex and increasingly changing.The old education theory and the education model are greatly impacted,and it is difficult to meet the challenge of social change.To adapt to the changing society,education must conform to the social changes,grasp the direction of change,to seek a new position.This paper uses the theory of"educational culture"to explain the change from the Grammar Translation Method(GTM)to the Communicative Language Teaching(CLT)method.Two different teaching methods are two different concepts,representing two different cultural connotations.

View Chinese & American Culture by VOM

The value orientation method provides a way to understand core culture differences well. Chinese culture and American culture are usually regarded as the representation of the eastern and western culture respectively. This paper tries to view Chinese and American Culture by Value Orientations Method to view these two cultures in details.

A Comparison of Cultures between China and the West——Taking Chinese Clothing and British Clothing as an Example

This paper attempts to do study on the comparison of differences between Chinese and western cultures by taking the Chinese and British clothing as an example. This comparison is made form two aspects about clothing culture. One is the separate characteristics of the Chinese and British clothing. The other is the common features of the Chinese and British clothing.The paper aims at making the Chinese confidently promote the clothing culture of China to make efforts to realize the great rejuvenation of China and enrich the culture of all mankind.

Studies on Genetic Transformatiom of Embryogenic Suspension Cultures of Sweetpotato

Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang was conducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase(GUS)and neomycin phosphotransferase(NPTⅡ)genes. The results indicated that embryogenic suspension cultures precultured for 1 - 3 d were suitable for the transformation. The optimal cocultivation time was 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1 for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100 mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.

Trans-Cultural Comparative Literature Method:Using Grammar Translation Techniques and Communicative Language Teaching Effectively

Trans-Cultural Comparative Literature Method originated from the discovery of common themes and viewpoints as comparing literary texts in different languages. This article explains how the method combine GT and CLT techniques in a lesson plan that engage students with activities that compare and contrast themes and cultural aspects found in two literary texts. It explores worldviews using information and knowledge of literary structures that motivate Chinese students.

A Phenotyping Protocol for Detailed Evaluation of Apple Root Resistance Responses Utilizing Tissue Culture Micropropagated Apple Plants

In the post-genomics era, reliable phenotypes are considered the bottleneck for unraveling the genetic control over the biology of interest. Phenotyping resistance response of roots to infection by soilborne pathogen is more challenging compared to that of plant aerial parts. In additional to the hidden nature and small stature of fine roots where infection occurs, extra obstacles exist for rosaceae tree crops such as apple. Due to self-incompatible reproduction and high-level heterozygosity of apple genome, genetically identical apple plants cannot be produced through apple seed germination. Here we report an established phenotyping protocol which includes a streamlined tissue culture procedure for micropropagation of uniform apple plants, standardized inoculation procedure using Pythium ultimum, and multilayered evaluating methods on apple root resistance traits. Because of the implementation of tissue culture based micropropagation procedure, constant availability of the uniform plants with defined genetic background, equivalent age and non-contaminated roots overcame a longstanding barrier of systematic and detailed phenotypic characterization of apple root resistance traits. Repeated infection assays by root-dipping inoculation demonstrated the reproducible and wide-range plant survival rates, from single-digit to over 90% survived plants for a given genotype. Genotype-specific values due to P. ultimum inoculation on shoot and root biomass reduction, maximum root lengths, leaf number and cumulative leaf areas were quantified between mock-inoculated and P. ultimum infected plants. Use of a glass-box container offered enhanced accessibility and minimized invasiveness for continuous and non-disruptive observation on the necrosis progression patterns along inoculated roots. With the assistance of a dissecting microscope, the genotype-specific resistance responses along the infected apple roots were captured and analyzed in detail. This reported phenotyping protocol represents a major development and should be easily adopted for other rosacea tree fruit crops with minor modifications.

Different Preservation Methods for Long Term Maintenance of <i>Haemophilus influenzae</i>

Haemophilus species are Gram-negative coccobacilli that require factor X and factor V for growth. Beyond this, it is a finicky bacterium to culture, and any modification of culture procedures greatly reduces isolation rates. Poor quality of laboratories in developing countries results in its poor isolation rates. This study was done with the objective of finding out the optimal cultural environment and media so that it could be maintained for a longer period in economical settings like ours which was done using H. influenzae ATCC 49,766. In this study, several culture media were tested as a means to preserve H. influenzae ATCC like TSB + glycerol + sheep blood, BHI broth, BHI broth + glycerol, BHI broth+ glycerol + sheep blood, Chocolate agar slant and satellitism plate. Three sets of respective media were inoculated with 18 - 24 hours growth of H. influenzae. They were incubated at 37?oC 48 hours in a candle extinction jar. The media were checked for growth by subculturing them on chocolate agar plates and identified by biochemical reactions. Each set was maintained at 2 oC - 8?oC, -20?oC and at room temperature and checked for the viability 24 hourly by subculturing them on chocolate agar. Results showed best growth of H. influenza on chocolate agar slants for 15 - 20 days, followed by BHI + glycerol + sheep blood broth and satellitism plate for 4 - 6 days followed by BHI broth for 2 - 4 days. There was no growth in TSB + glycerol + sheep blood broth and BHI + glycerol broth media. Present study showed similar results as done by NS Srikanth et al. 2003 with growth on chocolate agar & satellitism plate for 3 - 5 days but no growth in TSB + Glycerol + Sheep blood broth media. Chocolate agar slant is by far the most long term preserving media for H. influenzae. However, growth on BHI broth with various modifications is also showed a good preservation for 3 - 5 days, so with further experiments we can hope to maintain the organism in these media also.

Methods to produce induced pluripotent stem cell-derived mesenchymal stem cells: Mesenchymal stem cells from induced pluripotent stem cells

Mesenchymal stem cells(MSCs)have received significant attention in recent years due to their large potential for cell therapy.Indeed,they secrete a wide variety of immunomodulatory factors of interest for the treatment of immune-related disorders and inflammatory diseases.MSCs can be extracted from multiple tissues of the human body.However,several factors may restrict their use for clinical applications:the requirement of invasive procedures for their isolation,their limited numbers,and their heterogeneity according to the tissue of origin or donor.In addition,MSCs often present early signs of replicative senescence limiting their expansion in vitro,and their therapeutic capacity in vivo.Due to the clinical potential of MSCs,a considerable number of methods to differentiate induced pluripotent stem cells(iPSCs)into MSCs have emerged.iPSCs represent a new reliable,unlimited source to generate MSCs(MSCs derived from iPSC,iMSCs)from homogeneous and well-characterized cell lines,which would relieve many of the above mentioned technical and biological limitations.Additionally,the use of iPSCs prevents some of the ethical concerns surrounding the use of human embryonic stem cells.In this review,we analyze the main current protocols used to differentiate human iPSCs into MSCs,which we classify into five different categories:MSC Switch,Embryoid Body Formation,Specific Differentiation,Pathway Inhibitor,and Platelet Lysate.We also evaluate common and method-specific culture components and provide a list of positive and negative markers for MSC characterization.Further guidance on material requirements to produce iMSCs with these methods and on the phenotypic features of the iMSCs obtained is added.The information may help researchers identify protocol options to design and/or refine standardized procedures for large-scale production of iMSCs fitting clinical demands.