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In vitro study on the transmission of multidrug-resistant bacteria from textiles to pig skin
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作者 Pavlina Lena Spyridon Karageorgos +5 位作者 Maria Liatsou Aris P Agouridis Nikolaos Spernovasilis Demetris Lamnisos Panagiotis Papageorgis Constantinos Tsioutis 《World Journal of Experimental Medicine》 2023年第5期134-141,共8页
BACKGROUND The survival of microorganisms on textiles and specifically on healthcare profes-sionals’(HCP)attire has been demonstrated in several studies.The ability of microorganisms to adhere and remain on textiles ... BACKGROUND The survival of microorganisms on textiles and specifically on healthcare profes-sionals’(HCP)attire has been demonstrated in several studies.The ability of microorganisms to adhere and remain on textiles for up to hours or days raises questions as to their possible role in transmission from textile to skin via HCP to patients.AIM To evaluate the presence,survival and transmission of different multidrug-resistant bacteria(MDRB)from HCP attire onto skin.METHODS Three MDRB[methicillin-resistant Staphylococcus aureus(MRSA);vancomycin-resistant Enterococcus faecium(VRE);carbapenem-resistant Klebsiella pneumoniae,(CRKP)]were inoculated on textiles from scrubs(60%cotton-40%polyester)and white coat(100%cotton)at concentrations of 108 colony-forming units(CFU),105 CFU,and 103 CFU per mL.The inoculation of swatches was divided in time intervals of 1 min,5 min,15 min,30 min,1 h,2 h,3 h,4 h,5 h,and 6 h.At the end of each period,textiles were imprinted onto pig skins and each skin square was inverted onto three different selective chromogenic media.Growth from the pig skin squares was recorded for the 3 MDRB at the three above concentrations,for the whole length of the 6-h experiment.RESULTS MRSA was recovered from pig skins at all concentrations for the whole duration of the 6-h study.VRE was recovered from the concentration of 108 CFU/mL for 6 h and from 105 CFU/mL for up to 3 h,while showing no growth at 103 CFU/mL.CRKP was recovered from 108 CFU/mL for 6 h,up to 30 min from 105 CFU/mL and for 1 min from the concentration of 103 CFU/mL.CONCLUSION Evidence from the current study shows that MRSA can persist on textiles and transmit to skin for 6 h even at low concentrations.The fact that all MDRB can be sustained and transferred to skin even at lower concentrations,supports that textiles are implicated as vectors of bacterial spread. 展开更多
关键词 TEXTILES attire Multidrug-resistant bacteria Methicillin-resistant Staphylococcus aureus Vancomycin-resistant Enterococcus faecium Extended-spectrum b-lactamase Pig skin SKIN TRANSMISSION
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Elizabeth I:Gender and Paradigm
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作者 Rebecca Dean 《Journal of Literature and Art Studies》 2018年第12期1713-1720,共8页
Elizabeth I:Gender and Paradigm explores how Elizabeth I combined elements:male discourse,Elizabethan theatre,and the new Anglican church to maintain her power and rule for 45 years.The historical figure,Elizabeth,and... Elizabeth I:Gender and Paradigm explores how Elizabeth I combined elements:male discourse,Elizabethan theatre,and the new Anglican church to maintain her power and rule for 45 years.The historical figure,Elizabeth,and her dramatic double,Rosalind,provide feminist studies with an insight into how British women of the 16th century used contemporary notions of masculinity and femininity to their advantage.Elizabethan studies and its contemporary plays offer examples of fluid gender identity used by females for safety,free speech,and self-determination.Elizabeth used the new Anglican church as divine validation for her rule and the fact that Elizabeth spoke directly to her people and her parliament and conducted international relations in at least six languages.Elizabeth I,and the theatre she so enjoyed,offer a dialectic for tracing the lineage of what is now referred to as feminism. 展开更多
关键词 Elizabeth I GENDER identity attire SHAKESPEARE FEMINISM DISCOURSE women LEADERSHIP
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生长素结合蛋白AtTIR1和IAA28的原核表达及纯化 被引量:1
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作者 赵欣 刘会珍 +2 位作者 罗为桂 苏益 蔺万煌 《湖南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2013年第6期609-614,共6页
以pGEX–KG为骨架,运用分子克隆方法构建了生长素结合蛋白AtTIR1和IAA28的原核表达载体,通过转化不同表达菌株探索了2种蛋白的诱导表达条件。结果表明:在0.4 mmol/L IPTG诱导下,GST–IAA28融合蛋白在表达菌株Tuner中的表达量最高,其适... 以pGEX–KG为骨架,运用分子克隆方法构建了生长素结合蛋白AtTIR1和IAA28的原核表达载体,通过转化不同表达菌株探索了2种蛋白的诱导表达条件。结果表明:在0.4 mmol/L IPTG诱导下,GST–IAA28融合蛋白在表达菌株Tuner中的表达量最高,其适宜诱导温度为16℃;在0.6 mmol/L IPTG诱导下,BL21(DE3)中GST–IAA28的表达量最高;在各种浓度的IPTG诱导下,Rosetta中的GST–IAA28的产量均不高;在25℃和0.4mmol/L IPTG诱导条件下,Rosetta中的GST–AtTIR1表达总量最高,每克细菌可诱导出约0.2 mg的目标蛋白,但BL21(DE3)菌种中的GST–AtTIR1表达量很低。利用GST SefiroseTM resin亲和树脂对表达的蛋白进行纯化,获得了较纯的AtTIR1和IAA28蛋白。 展开更多
关键词 生长素结合蛋白 AtTIR1基因 IAA28基因 原核表达 蛋白质纯化
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Between“Guandai”(冠带)and“Yidi”(夷狄):Sima Qian's National Consciousness
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作者 Zijin WANG Ruli WANG 《Journal of the Chinese Nation Studies》 2023年第1期151-176,共26页
During the reign of Emperor Wu of Han Dynasty,the ethnic relations in China entered a new historical stage.Records of the Historian written during this period gives a basically factual account of the ethnic history in... During the reign of Emperor Wu of Han Dynasty,the ethnic relations in China entered a new historical stage.Records of the Historian written during this period gives a basically factual account of the ethnic history in China since ancient epochs.It is the first specialized work on ethnic history.The national consciousness that Sima Qian presents in this work is distinctively different from the Confucian tradition,which draws a strict line between Hua(华,China)and Yi(夷,the barbarians).His attention on the commonality of the cultural genes between the farming peoples of the Central-Plains and the nomadic hunting peoples of the borderlands,his recognition of the integrated impact that multiple ethnic groups have on the rise and development of Huaxia(华夏,Chinese)civilization,his respect for"differences among barbarians of the four directions"(四夷各异),and his approval of the“merits of Hu attire”(胡服之功)are all embodiment of his liberal views.To explore the formation and development of the cultural community of the Chinese nation and to understand and interpret this historical process of ethnic integration,we should attach great importance to the positive significance of Sima Qian's national consciousness. 展开更多
关键词 Records of the Historian Guandai Yidi cultural genes the merits of Hu attire Li Ling differences among barbarians of the four directions
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