Design and Implementation of USB Key System Based on Dual-Factor Identity Authentication Protocol

With the increasing demand for information security,traditional single-factor authentication technology can no longer meet security requirements.To this end,this paper proposes a Universal Serial Bus(USB)Key hardware and software system based on a two-factor authentication protocol,aiming to improve the security and reliability of authentication.This paper first analyzes the current status and technical principles of USB Key-related research domestically and internationally and designs a two-factor authentication protocol that combines impact/response authentication and static password authentication.The system consists of a host computer and a USB Key device.The host computer interacts with the USB Key through a graphical user interface.The Secure Hash Algorithm 1(SHA-1)and MySQL database are used to implement the authentication function.Experimental results show that the designed two-factor authentication protocol can effectively prevent replay attacks and information tampering,and improve the security of authentication.If the corresponding USB Key is not inserted,the system will prompt that the device is not found.Once the USB Key is inserted,user identity is confirmed through two-factor verification,which includes impact/response authentication and static password authentication.

The molecular characteristics of soybean ARR-B transcription factors

The Type-B authentic response regulator(ARR-Bs)gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes.However,study of ARR-Bs gene family in soybean is limited.Genome-wide analysis and expression profiling of the ARR-Bs gene family were performed in the soybean genome.31 ARR-Bs genes(namely GmARR-B1-31)were identified,containing conserved catalytic domains with protein lengths and molecular weights ranging from 246 to 699 amino acids(aa)and 28.30 to 76.86 kDa,respectively.Phylogenetic analysis grouped ARR-Bs genes into three clusters—Cluster I,Cluster II,and Cluster III—which included 15,12,and 4 genes,respectively,and were asymmetrically distributed on 17 chromosomes.Tissue specific expression analysis of GmARR-Bs family revealed a high transcription level in flowers,roots and seeds.The subcellular localization of GmARR5,GmARR14 were observed in the nucleus,and the promoter region of them included low-temperature responsive element(LTR),the C-repeat/dehydration responsive element(DRE),MBS,ABRE,MYB,MEJA and TCA-elements,which possibly participate in abiotic stress and hormones responses.qRTPCR analysis showed that the expression of GmARR-B genes were affected by different abiotic stresses,especially cold stress and salt stress,and GmARR-B5 and GmARR-B14 were significantly induced by cold stress.This suggested that ARR-Bs genes were involved in multiple abiotic stress response pathways and acted as a positive factor under cold stress.