Effect of adjuvant ozone autohemotherapy combined with naloxone therapy on respiratory function and neurological function in COPD patients with pulmonary encephalopathy

Objective:To study the effect of adjuvant ozone autohemotherapy combined with naloxone therapy on respiratory function and neurological function in COPD patients with pulmonary encephalopathy.Methods: A total of 30 COPD patients with pulmonary encephalopathy who were treated in our hospital between May 2013 and January 2016 were collected and divided into the control group and observation group (n=15) according to the random number table. Control group of patients received conventional treatment + naloxone therapy, observation group of patients received conventional treatment + ozone autohemotherapy combined with naloxone therapy, and the therapies lasted for 10 d. Differences in the levels of arterial blood gas indexes, peripheral blood nerve injury indexes and cerebrospinal fluid nerve autophagy indexes were compared between two groups of patients before and after treatment.Results:Before treatment, differences in the levels of arterial blood gas indexes, peripheral blood nerve injury indexes and cerebrospinal fluid nerve autophagy indexes were not statistically significant between two groups of patients. After treatment, the arterial blood gas pH value and PaO2 level of observation group were higher than those of control group while PaCO2 level was lower than that of control group;peripheral blood nerve injury indexes S100β, NSE and MBP contents were lower than those of control group;cerebrospinal fluid neural apoptosis indexesβ-GBA and Atg7 contents were lower than those of control group.Conclusion: Adjuvant ozone autohemotherapy combined with naloxone therapy can optimize the respiratory function and reduce the nerve injury in COPD patients with pulmonary encephalopathy.

Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice

Objective: To evaluate the therapeutic effects of acupoint autohemotherapy(A-AHT) on 1-chloro-2,4-dinitrobenzene(DNCB)-induced atopic dermatitis(AD) in mice focusing on regulating T helper 1/T helper 2(Th1/Th2) immune responses. Methods: Thirty BALB/c mice were divided into 5 groups by a random number table, including normal control(NC), AD model(AD), A-AHT, sham A-AHT(sA-AHT), and acupoint injection of normal saline(A-NS) groups, 6 mice per group. Mice were challenged by DNCB for the establishment of experimental AD model. On the 8th day, except for the NC and AD groups, the mice in the other groups received management once every other day for a total of 28 days. For the A-AHT and sA-AHT groups, 0.05 mL of autologous whole blood(AWB) was injected into bilateral Zusanli(ST 36) and Quchi(LI 11) and sham-acupoints(5 mm lateral to ST 36 and LI 11), respectively. The A-NS group was administrated with 0.05 m L of normal saline by acupoint injection into ST 36 and LI 11. Dermatitis severity for dorsal skin of mice was determined using the Severity Scoring of Atopic Dermatitis(SCORAD) every week. The total immunoglobulin E(IgE), interleukin-4(IL-4), and interferon-gamma(IFN-γ) cytokine levels in serum were examined by enzyme-linked immunosorbent assay(ELISA). Spleen Th1/Th2 expression were analyzed via flow cytometry and immunohistochemical assay was used to detect T-box expressed in T cell(T-bet) and GATA-binding protein 3(GATA3) expressions in skin lesions of mice. Results: Compared with the AD group, both A-AHT and sA-AHT reduced the SCORAD index and serum IgE level(P<0.05 or P<0.01);A-AHT, sA-AHT and A-NS down-regulated serum IL-4 level and upregulated IFN-γ/IL-4 ratio(P<0.05 or P<0.01);A-AHT regulated the Th1/Th2 shift specifically and increased the related transcription factors such as T-bet expression and T-bet/GATA3 ratio(P<0.05). Conclusion: A-AHT showed significant effectiveness on the AD model mice, through regulating Th1/Th2 immune responses.

Successfully treated recalcitrant atopic eczema with acupoint autohemotherapy:A case report and hypothesized mechanism of the therapy

Acupoint autohemotherapy at bilateral Zusanli(ST36)and Xuehai(SP10)was used to treat a 26-year-old female patient who had suffered from recalcitrant atopic eczema(AE)for five years.The treatment was applied at a frequency of once per week for the first month,followed by a three-month period of once every other week.At the end of treatment,the patient’s AE symptoms were entirely resolved,and by the end of a six-month follow-up her immunoglobulin E level had returned to the normal range.Further,there was no relapse of AE symptoms during the six-month follow-up.Therefore,we hypothesized that after the repeated treatments the local inflammatory reaction induced by autologous blood injection triggered a local immune response,followed by a systemic immune response after the repeated treatment,finally leading to the anti-inflammation and immunomodulation effects.This case suggests that acupoint autohemotherapy could be used as an effective complementary treatment for recalcitrant AE,especially in cases where other treatments have failed.Further comparative studies are needed to corroborate the value and mechanisms of this therapy.

Ozone Therapy: A Critical Physiological and Diverse Clinical Evaluation with Regard to Immune Modulation, Anti-Infectious Properties, Anti-Cancer Potential, and Impact on Anti-Oxidant Enzymes

Ozone therapy has been marred by conventional science for years due to many flawed experimental designs or small sample sizes of the population in which it intends to study. For this reason, many physicians have dismissed ozone therapy and limited funds have been delegated to furthering the knowledge of its therapeutic effects. However, there is evidence that suggests that ozone does have various therapeutic effects that range from disinfection of pathogens, anticancer properties, and treatment of back ailments. In this paper, we have collected the more promising studies that suggest the efficacy and safety of ozone therapy primarily focusing on autohemotherapy. This paper is designed to promote awareness of ozone therapy and to show some supporting evidence of its efficacy. Clinically, ozone therapy is often used adjunctively and combined with other treatment modalities to enhance or encourage a desired mechanism of action. Since the efficacy of ozone alone is still contentious, it is important to note that ozone therapy should be used in conjunction with other various treatments with very few exceptions.