Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regen...Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.展开更多
In order to improve the efficiency of automatic warehouse control system,the experimental platform of stereoscopic warehouse with s7-1500plc is designed.The manipulator is driven by stepper motor and servo motor to re...In order to improve the efficiency of automatic warehouse control system,the experimental platform of stereoscopic warehouse with s7-1500plc is designed.The manipulator is driven by stepper motor and servo motor to realize x,y and Z three-axis space motion.The material transmission system is built by general-purpose G120 inverter.HMI KTP700 realizes control and status monitoring.The materials are identified and classified by RFID sensor and other sensors.TIAV15 software build PROFINET communication and PROFIBUS communication network.Using the GRAPH language programming can improve the visualization degree of application and solve the complex problems of program design and debugging of the warehouse control system.Through the design of hardware and software,a set of complete control system design scheme is formed,which has high practical value and provides an excellent teaching and experiment platform for the intelligent storage system.展开更多
A novel technique for automatic seismic data processing using both integral and local feature of seismograms was presented in this paper. Here, the term integral feature of seismograms refers to feature which may depi...A novel technique for automatic seismic data processing using both integral and local feature of seismograms was presented in this paper. Here, the term integral feature of seismograms refers to feature which may depict the shape of the whole seismograms. However, unlike some previous efforts which completely abandon the DIAL approach, i.e., signal detection, phase identifi- cation, association, and event localization, and seek to use envelope cross-correlation to detect seismic events directly, our technique keeps following the DIAL approach, but in addition to detect signals corresponding to individual seismic phases, it also detects continuous wave-trains and explores their feature for phase-type identification and signal association. More concrete ideas about how to define wave-trains and combine them with various detections, as well as how to measure and utilize their feature in the seismic data processing were expatiated in the paper. This approach has been applied to the routine data processing by us for years, and test results for a 16 days＇ period using data from the Xinjiang seismic station network were presented. The automatic processing results have fairly low false and missed event rate simultaneously, showing that the new technique has good application prospects for improvement of the automatic seismic data processing.展开更多
The closed-loop reservoir management technique enables a dynamic and real-time optimal production schedule under the existing reservoir conditions to be achieved by adjusting the injection and production strategies. T...The closed-loop reservoir management technique enables a dynamic and real-time optimal production schedule under the existing reservoir conditions to be achieved by adjusting the injection and production strategies. This is one of the most effective ways to exploit limited oil reserves more economically and efficiently. There are two steps in closed-loop reservoir management： automatic history matching and reservoir production opti- mization. Both of the steps are large-scale complicated optimization problems. This paper gives a general review of the two basic techniques in closed-loop reservoir man- agement; summarizes the applications of gradient-based algorithms, gradient-free algorithms, and artificial intelligence algorithms; analyzes the characteristics and application conditions of these optimization methods; and finally discusses the emphases and directions of future research on both automatic history matching and reservoir production optimization.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82271397(to MG),82001293(to MG),82171355(to RX),81971295(to RX)and 81671189(to RX)。
文摘Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.
文摘In order to improve the efficiency of automatic warehouse control system,the experimental platform of stereoscopic warehouse with s7-1500plc is designed.The manipulator is driven by stepper motor and servo motor to realize x,y and Z three-axis space motion.The material transmission system is built by general-purpose G120 inverter.HMI KTP700 realizes control and status monitoring.The materials are identified and classified by RFID sensor and other sensors.TIAV15 software build PROFINET communication and PROFIBUS communication network.Using the GRAPH language programming can improve the visualization degree of application and solve the complex problems of program design and debugging of the warehouse control system.Through the design of hardware and software,a set of complete control system design scheme is formed,which has high practical value and provides an excellent teaching and experiment platform for the intelligent storage system.
文摘A novel technique for automatic seismic data processing using both integral and local feature of seismograms was presented in this paper. Here, the term integral feature of seismograms refers to feature which may depict the shape of the whole seismograms. However, unlike some previous efforts which completely abandon the DIAL approach, i.e., signal detection, phase identifi- cation, association, and event localization, and seek to use envelope cross-correlation to detect seismic events directly, our technique keeps following the DIAL approach, but in addition to detect signals corresponding to individual seismic phases, it also detects continuous wave-trains and explores their feature for phase-type identification and signal association. More concrete ideas about how to define wave-trains and combine them with various detections, as well as how to measure and utilize their feature in the seismic data processing were expatiated in the paper. This approach has been applied to the routine data processing by us for years, and test results for a 16 days＇ period using data from the Xinjiang seismic station network were presented. The automatic processing results have fairly low false and missed event rate simultaneously, showing that the new technique has good application prospects for improvement of the automatic seismic data processing.
基金the Important National Science & Technology Specific Projects of China (Grant No. 2011ZX05024-004)the Natural Science Foundation for Distinguished Young Scholars of Shandong Province, China (Grant No. JQ201115)+2 种基金the Program for New Century Excellent Talents in University (Grant No. NCET-11-0734)the Fundamental Research Funds for the Central Universities (Grant No. 13CX05007A, 13CX05016A)the Program for Changjiang Scholars and Innovative Research Team in University (IRT1294)
文摘The closed-loop reservoir management technique enables a dynamic and real-time optimal production schedule under the existing reservoir conditions to be achieved by adjusting the injection and production strategies. This is one of the most effective ways to exploit limited oil reserves more economically and efficiently. There are two steps in closed-loop reservoir management： automatic history matching and reservoir production opti- mization. Both of the steps are large-scale complicated optimization problems. This paper gives a general review of the two basic techniques in closed-loop reservoir man- agement; summarizes the applications of gradient-based algorithms, gradient-free algorithms, and artificial intelligence algorithms; analyzes the characteristics and application conditions of these optimization methods; and finally discusses the emphases and directions of future research on both automatic history matching and reservoir production optimization.
