[ Objective] To develop an efficient and practical method for detecting avermectin residue in animal-derived food. [ Method ] Acetonitrile was used as the extraction fluid. After the removal of moisture, trifluoroacet...[ Objective] To develop an efficient and practical method for detecting avermectin residue in animal-derived food. [ Method ] Acetonitrile was used as the extraction fluid. After the removal of moisture, trifluoroacetic anhydride and N-methyl imidazole were used for derivatization and mixed well. After complete derivatization, high-performance liquid chromatography （HPLC） with C18 column was used for accurate qualitative and quantitative analysis of the detected substance using methanol aqueous solution （92： 8, V/ V）. [ Result] The detection limit of HPLC connected with a fluorescence detector was up to 0.1 ug/L. When the standard was added to the substrate at a final concentration in the range of 0.1 -20.0 lag/L, the recoveries were in the range of 80% -108% and the relative deviation was in the range of 4.1% -11.4%. [ Conclusion] The developed HPLC con- nected with a fluorescence detector is simple, practical, highly sensitive and resistant to interference, and it thus can meet the needs for determina- tion of biological toxin residues of avermectins in animal-derived food in laboratory.展开更多
文摘[ Objective] To develop an efficient and practical method for detecting avermectin residue in animal-derived food. [ Method ] Acetonitrile was used as the extraction fluid. After the removal of moisture, trifluoroacetic anhydride and N-methyl imidazole were used for derivatization and mixed well. After complete derivatization, high-performance liquid chromatography （HPLC） with C18 column was used for accurate qualitative and quantitative analysis of the detected substance using methanol aqueous solution （92： 8, V/ V）. [ Result] The detection limit of HPLC connected with a fluorescence detector was up to 0.1 ug/L. When the standard was added to the substrate at a final concentration in the range of 0.1 -20.0 lag/L, the recoveries were in the range of 80% -108% and the relative deviation was in the range of 4.1% -11.4%. [ Conclusion] The developed HPLC con- nected with a fluorescence detector is simple, practical, highly sensitive and resistant to interference, and it thus can meet the needs for determina- tion of biological toxin residues of avermectins in animal-derived food in laboratory.
