One caveat to the dinosaur’s extinction is the conclusion that avian dinosaurs survived and became ancestors of birds. Their mobility enabled them to migrate great distances and find the nutrients needed to survive. ...One caveat to the dinosaur’s extinction is the conclusion that avian dinosaurs survived and became ancestors of birds. Their mobility enabled them to migrate great distances and find the nutrients needed to survive. Given this scenario, could the current observable migration of birds (the “dinosaurian offspring”) now be related? Migration is the regular seasonal movement undertaken by many species of birds, with the most common pattern, flying north in the Northern spring to breed in the temperate or Arctic summer and returning in the Northern autumn to wintering grounds in warmer regions of the south. The primary motivation for migration appears to be food. None of the major North-South migratory pathways fly over the Caribbean but three main fly ways, past to the west of the theorized K-T impact centre. Due to their ability to fly, the “avian Dinosaurs” adapted and survived very quickly in response to the disaster that marked the K-T boundary. It is an interesting speculation that the avian migration that we witness today is rooted in an event that occurred 66 million years ago! But it does explain why the migratory birds mostly fly from Polar summer to polar summer when they could just be as easily fly from Polar zone to the warmer equatorial region and back. In the recent article in Nature by Melanie During about identifying the late spring timing of the “Astro disaster”, it can be cited as consistent with my speculation. A late April early May Impact as suggested by During would have seen these migrations completely. The western migratory routes would have been found to be “luxurious” in vegetation in that first northern autumn after the “Astro-impact” while all eastern routes would have still been barren.展开更多
The stamping-out strategy has been used to control highly pathogenic avian influenza viruses in many countries,driven by the belief that vaccination would not be successful against such viruses and fears that avian in...The stamping-out strategy has been used to control highly pathogenic avian influenza viruses in many countries,driven by the belief that vaccination would not be successful against such viruses and fears that avian influenza virus in vaccinated birds would evolve more rapidly and pose a greater risk to humans.In this review,we summarize the successes in controlling highly pathogenic avian influenza in China and make suggestions regarding the requirements for vaccine selection and effectiveness.In addition,we present evidence that vaccination of poultry not only eliminates human infection with avian influenza virus,but also significantly reduces and abolishes some harmful characteristics of avian influenza virus.展开更多
Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the domi...Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.展开更多
H7N9 subtype avian influenza virus poses a great challenge for poultry industry.Newcastle disease virus(NDV)-vectored H7N9 avian influenza vaccines(NDV_(vec)H7N9)are effective in disease control because they are prote...H7N9 subtype avian influenza virus poses a great challenge for poultry industry.Newcastle disease virus(NDV)-vectored H7N9 avian influenza vaccines(NDV_(vec)H7N9)are effective in disease control because they are protective and allow mass administration.Of note,these vaccines elicit undetectable H7N9-specific hemagglutination-inhibition(HI)but high IgG antibodies in chickens.However,the molecular basis and protective mechanism underlying this particular antibody immunity remain unclear.Herein,immunization with an NDV_(vec)H7N9 induced low anti-H7N9 HI and virus neutralization titers but high levels of hemagglutinin(HA)-binding IgG antibodies in chickens.Three residues(S150,G151 and S152)in HA of H7N9 virus were identified as the dominant epitopes recognized by the NDV_(vec)H7N9 immune serum.Passively transferred NDV_(vec)H7N9 immune serum conferred complete protection against H7N9 virus infection in chickens.The NDV_(vec)H7N9 immune serum can mediate a potent lysis of HA-expressing and H7N9 virus-infected cells and significantly suppress H7N9 virus infectivity.These activities of the serum were significantly impaired after heat-inactivation or treatment with complement inhibitor,suggesting the engagement of the complement system.Moreover,mutations in the 150-SGS-152 sites in HA resulted in significant reductions in cell lysis and virus neutralization mediated by the NDV_(vec)H7N9 immune serum,indicating the requirement of antibody-antigen binding for complement activity.Therefore,antibodies induced by the NDV_(vec)H7N9 can activate antibody-dependent complement-mediated lysis of H7N9 virus-infected cells and complement-mediated neutralization of H7N9 virus.Our findings unveiled a novel role of the complement in protection conferred by the NDV_(vec)H7N9,highlighting a potential benefit of engaging the complement system in H7N9 vaccine design.展开更多
Avian pathogenic Escherichia coli(APEC)belonging to extraintestinal pathogenic E.coli(ExPEC)can cause severe infections in extraintestinal tissues in birds and humans,such as the lungs and blood.MprA(microcin producti...Avian pathogenic Escherichia coli(APEC)belonging to extraintestinal pathogenic E.coli(ExPEC)can cause severe infections in extraintestinal tissues in birds and humans,such as the lungs and blood.MprA(microcin production regulation,locus A,herein renamed AbsR,a blood survival regulator),a member of the MarR(multiple antibiotic resistance regulator)transcriptional regulator family,governs the expression of capsule biosynthetic genes in human ExPEC and represents a promising druggable target for antimicrobials.However,a deep understanding of the AbsR regulatory mechanism as well as its regulon is lacking.In this study,we present a systems-level analysis of the APEC AbsR regulon using ChIP-Seq(chromatin immunoprecipitation sequencing)and RNA-Seq(RNA sequencing)methods.We found that AbsR directly regulates 99 genes and indirectly regulates 667 genes.Furthermore,we showed that:1)AbsR contributes to antiphagocytotic effects by macrophages and virulence in a mouse model for systemic infection by directly activating the capsular gene cluster;2)AbsR positively impacts biofilm formation via direct regulation of the T2SS(type II secretion system)but plays a marginal role in virulence;and 3)AbsR directly upregulates the acid tolerance signaling system EvgAS to withstand acid stress but is dispensable in ExPEC virulence.Finally,our data indicate that the role of AbsR in virulence gene regulation is relatively conserved in ExPEC strains.Altogether,this study provides a comprehensive analysis of the AbsR regulon and regulatory mechanism,and our data suggest that AbsR likely influences virulence primarily through the control of capsule production.Interestingly,we found that AbsR severely represses the expression of the type I-F CRISPR(clustered regularly interspaced short palindromic repeats)-Cas(CRISPR associated)systems,which could have implications in CRISPR biology and application.展开更多
Avian embryos,which develop within eggs,exhibit remarkable tolerance to extremely low temperatures.Despite being a common trait among all birds,the mechanisms underlying this cold tolerance in avian embryos remain lar...Avian embryos,which develop within eggs,exhibit remarkable tolerance to extremely low temperatures.Despite being a common trait among all birds,the mechanisms underlying this cold tolerance in avian embryos remain largely unknown.To gain a better understanding of this phenomenon and the coping mechanisms involved,we reviewed the literature on severe cold tolerance in embryos of both wild and domestic birds.We found that embryos of different bird orders exhibit tolerance to severe cold during their development.In response to cold stress,embryos slow down their heartbeat rates and metabolism.In severe cold temperatures,embryos can suspend these processes,entering a torpid-like state of cardiac arrest.To compensate for these developmental delays,embryos extend their regular incubation periods.Depending on their embryonic age,embryos of all bird species can tolerate acute severe cold regimes;only a few tolerate chronic severe cold regimes.We also discussed various extrinsic and intrinsic factors that affect the tolerance of bird embryos to low temperatures before and after incubation.Cold tolerance appears to be a heritable trait shared by wild and domestic embryos of all bird classes,regardless of egg size or development(altricial/precocial).Driven by environmental variability,cold tolerance in avian embryos is an optimal physiological and ecological strategy to mitigate the adverse effects of cold conditions on their development in response to fluctuating environmental temperatures.展开更多
Background The fat deposition has a crucial role in animal meat flavor,and fat deposition-related traits are vital for breeding in the commercial duck industry.Avian fat-related traits are typical complex phenotypes,w...Background The fat deposition has a crucial role in animal meat flavor,and fat deposition-related traits are vital for breeding in the commercial duck industry.Avian fat-related traits are typical complex phenotypes,which need a large amount of data to analyze the genetic loci.Results In this study,we performed a new phenotypic analysis of fat traits and genotyped whole-genome varia-tions for 1,246 ducks,and combed with previous GWAS data to reach 1,880 ducks for following analysis.The carcass composition traits,subcutaneous fat weight(SFW),subcutaneous fat percentage(SFP),abdominal fat weight(AFW),abdominal fat percentage(AFP)and the body weight of day 42(BW42)for each duck were collected.We identified a set of new loci that affect the traits related to fat deposition in avian.Among these loci,ceroid-lipofuscinosis,neuronal 8(CLN8)is a novel candidate gene controlling fat deposition.We investigated its novel function and regulation in avian adipogenesis.Five significant SNPs(the most significant SNP,P-value=21.37E-12)and a single haplotype were detected in the upstream of CLN8 for subcutaneous fat percentage.Subsequently,luciferase assay demonstrated that 5 linked SNPs in the upstream of the CLN8 gene significantly decreased the transcriptional activity of CLN8.Further,ATAC-seq analysis showed that transcription factor binding sites were identified in a region close to the haplotype.A set of luciferase reporter gene vectors that contained different deletion fragments of the CLN8 promoter were con-structed,and the core promoter area of CLN8 was finally identified in the-1,884/-1,207 bp region of the 5′flanking sequences,which contains adipogenesis-related transcription factors binding sites.Moreover,the over-expression of CLN8 can remarkably facilitate adipocyte differentiation in ICPs.Consistent with these,the global transcriptome profiling and functional analysis of the over-expressed CLN8 in the cell line further revealed that the lipid biosynthetic process during the adipogenesis was significantly enriched.Conclusions Our results demonstrated that CLN8 is a positive regulator of avian adipocyte differentiation.These findings identify a novel function of CLN8 in adipocyte differentiation,which provides important clues for the further study of the mechanism of avian fat deposition.展开更多
Recent studies have revealed concerning declines in bird populations in Europe and other parts of the world.In understudied but biodiverse regions,especially those that are located along key migratory flyways,there is...Recent studies have revealed concerning declines in bird populations in Europe and other parts of the world.In understudied but biodiverse regions,especially those that are located along key migratory flyways,there is an unmet need to evaluate the status of resident and migratory birdlife for avian conservation ecology.This is especially urgent at a time when recent regional studies have highlighted the scale of the decline of the European and Palearctic avifauna.Rapidly growing community science datasets may help bridge the gaps in our understanding of long-term regional avifaunal trends.In this study,we used eBird data submitted by observers in Türkiye to undertake the first long-term assessment of the state of the country’s birds from 2001 to 2022.We evaluated population trends based on presence-absence data of species to determine if any birds have significantly increased or decreased in the country so far this century.We provide a list of trends for 418 bird species that had large enough sample sizes of eBird observations from Türkiye,and highlight which species have significantly declined or increased.We found that 63 species have significantly declined in detection while 120 have significantly increased during the study period.Our findings provide a baseline for the future monitoring of Türkiye’s birds and help contextualize population changes of birds in Türkiye against those occurring at the continental(Palearctic)scale.Additionally,we include movement classifications for all of the bird species in this study.We also provide a framework to effectively use eBird data to assess long-term avifaunal changes at the country-level in other understudied regions.Finally,we highlight ways in which eBirders in Türkiye and elsewhere can strengthen the quality and value of community ornithology data for population assessments and avian conservation ecology.展开更多
Avian malaria and related haemosporidian parasites can negatively impact fitness in many songbirds.Research on the malaria infection and its physiological costs on their avian hosts is heavily skewed toward native pas...Avian malaria and related haemosporidian parasites can negatively impact fitness in many songbirds.Research on the malaria infection and its physiological costs on their avian hosts is heavily skewed toward native passerines,with exotic species underrepresented.However,introduced species may carry on and spread new pathogens to native species,and play a role on parasite transmission cycle in invaded bird communities as pathogen reservoir.Here,we molecularly assess the prevalence and diversity of haemosporidian parasites in three introduced wetland passerines(the Red Avadavat Amandava amandava,the Yellow-crowned Bishop Euplectes afer,and the Common Waxbill Estrilda astrild)captured during the same season in southwestern Spain.We also explored the relation between parasite infection,body condition,haematocrit,and uropygial gland volume.We detected an overall parasite prevalence of 3.55%,where Common Waxbills showed higher prevalence(6.94%)than Red Avadavats(1.51%).None Yellow-crowned Bishops were infected with haemosporidians.Almost 60%of infections were caused by Leucocytozoon,and about 40%by Plasmodium.We identified four unique lineages of Plasmodium and three of Leucocytozoon.Moreover,91%of the identified host-parasite interactions represented new host records for these haemosporidian parasites.Parasite infection was not related to body condition,haematocrit,and uropygial gland volume of the wetland passerines.Haematocrit values varied seasonally among bird species.Additionally,haematocrit was positively related to body condition in the Yellow-crowned Bishops,but not in the other species.Red Avadavats had higher haematocrit levels than Yellow-crowned Bishops,whereas Common Waxbills showed the lower haematocrit values.The uropygial gland volume was positively correlated with body condition in all bird species.Common Waxbills showed higher uropygial gland volumes related to their body size than birds from other two species.These outcomes highlight the importance of exotic invasive species in the transmission dynamics of haemosporidian parasites.展开更多
BACKGROUND Most of the first symptoms of avian influenza are respiratory symptoms,and cases with occipital neuralgia as the first manifestation are rarely reported.CASE SUMMARY A middle-aged patient complaining of par...BACKGROUND Most of the first symptoms of avian influenza are respiratory symptoms,and cases with occipital neuralgia as the first manifestation are rarely reported.CASE SUMMARY A middle-aged patient complaining of paroxysmal pain behind the ear was admitted to our hospital.The patient’s condition changed rapidly,and high fever,unexpected respiratory failure,and multiple organ failure developed rapidly.The patient was diagnosed with H7N9 avian influenza based on etiology.CONCLUSION We believe that the etiology of occipital neuralgia is complex and could be the earliest manifestation of severe diseases.The possibility of an infectious disease should be considered when occipital neuralgia is accompanied by fever.Avian influenza is one of these causative agents.展开更多
Among the avian species, understanding the roles of activin happen to be a dominant challenge in genetic evolution due to its complexity in nature. A case study of the activin gene in avian species was carried out usi...Among the avian species, understanding the roles of activin happen to be a dominant challenge in genetic evolution due to its complexity in nature. A case study of the activin gene in avian species was carried out using bioinformatics. As a sedentary bird, guinea fowl is more susceptible to local selection processes and needs a proper genetic study for conservation. The present study provides the basis for the use of activin or its target genes for the improvement of impaired wound healing, and activin antagonists for the prevention and treatment of fibrosis and the end of malignant tumors that over-express activin. The information provided will serve as a basic tool for broader genetic diversity studies to identify valuable poultry genetic resources and major genes for the development of breeding programs. This study was done by retrieving hundred (100) nucleotides and amino acid sequences of the activin gene belonging to guinea fowl and other avians from the GeneBank, aligning the sequences using BlastP determined the percent identity and phylogenetic relationship of the activin gene of guinea fowl and other avians. The shortest activin nucleotide sequence (467 bp) was observed in chicken and the longest (39896445 bp) in duck. Using the comparative sequence analysis, it was observed that the activin gene of chickens, turkeys and guinea fowl shared percent identity ranging from 91% to 95%. The percent identity reflects the degree of relatedness of species. Although closely related (90%) in ancestral line, the activin gene of guinea fowl and quail cannot be compared with guinea fowl-turkey (95%) nor guinea fowl-chicken (90%), in both biological functions and evolutionary relationship. Finally, the percent identity and similarity in function of the activin gene of guinea fowl, turkey, and chicken were in the range of 93% - 100%, indicating that the activin gene of avians possesses similar functions, well conserved and is very effective in performing functions like increasing FSH bindings, FSH-induced aromatization, improves wound healing and enhances scar formation, regulates morphogenesis of branching organs, and enhances ovarian folliculogenesis. The study, therefore, recommends farmers select and breed for activin genes in order to promote reproductive efficiency, thereby barricading species extinction.展开更多
Global climate change affects many facets of avian ecology, such as shifts in breeding phenology and migration patterns. Migrating bird species respond to changes in climate by shifting their temporal patterns of spri...Global climate change affects many facets of avian ecology, such as shifts in breeding phenology and migration patterns. Migrating bird species respond to changes in climate by shifting their temporal patterns of spring migration. However, variation in species’ responses exists based on various life history traits, which exposes some species to an increased risk of phenological mismatch. This study examined the spring arrival dates of 115 migrating species over 127 years (1889-2015) using archival sources in West Virginia, USA, making this research unique in the length of study, the high number of species studied, and the historical crowd-sourced observations analyzed. Of the 115 taxa, 45 showed significant negative slopes of spring arrival dates (arriving earlier in the spring) plotted against the year. In contrast, only nine species showed positive slopes (arriving later in the spring), albeit non-significant. The average advance of spring arrival date for all species was 1.7 days per decade, and an advance of 2.6 days per decade in species that showed significance. Arrival dates were associated with increasing spring temperatures—for each 1˚C increase, the arrival date advanced by 0.81 days/decade. Several life history traits were linked to species that advanced their first arrival dates, including a shorter distance migrated to reach wintering grounds, increasing populations, and foraging habitat. Most avian species are advancing their spring arrival dates in response to climate change. However, the implications of earlier spring arrival are unclear. We draw attention to shifts in arrival dates and wintering ranges, leading to a possible increase in overwintering in the mid-latitudes of North America.展开更多
This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated...This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an Mqq- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MFIC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV wet enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on HgN2 infection was evaluated by an M]q- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to PIgN2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces HgN2 AIV replication and promotes early humoral immune responses in young chickens.展开更多
A novel avian influenza A (H7N9) virus was discovered in February 2013 in China and has resulted in more than 100 comfirmed human infections including 26 fatal cases as of May 2, 2013. The situation raises many ur- ...A novel avian influenza A (H7N9) virus was discovered in February 2013 in China and has resulted in more than 100 comfirmed human infections including 26 fatal cases as of May 2, 2013. The situation raises many ur- gent questions and global public health concerns. In this study, epidemiologic characteristics of infected human cases in Jiangsu province were analyzed and risk assessment was undertaken based on the information available. Briefly, it is highly unlikely that a pandemic of human infection with avian influenza A (HTN9) virus will happen in Jiangsu Province in the near future. Iia the end, some measures are recommended to prevent the situation from becoming worse.展开更多
216 avian pathogenic Escherichia coli (APEC) isolates were obtained from poultry with colibacillosis in different areas of China. Among them, 195 were serotyped as 078, 088, and 093. Thirteen virulence-associated ge...216 avian pathogenic Escherichia coli (APEC) isolates were obtained from poultry with colibacillosis in different areas of China. Among them, 195 were serotyped as 078, 088, and 093. Thirteen virulence-associated genes, including fimC, iucD, iss, tsh, fyuA, irp2, eaeA, hlyE, colV, papC, stx2f, vat, and astA, were submitted to PCR amplification. The fimC gene was the most prevalent with a detection rate of 93.6%, followed by iucD (70.8%), iss (58.8%), and tsh (51.4%) in APEC isolates. The detection rate of high pathogenicity islands (HPI)-associatedfyuA and irp2 genes were both 44.9%, with no LEE (the locus of enterocyte effacement) island-associated gene eaeA detected. In terms of distribution patterns of the 13 virulence-associated genes, 5 isolates harborbed 10 genes, 19 isolates contained onlyfimC gene, and only 4 isolates had no virulence-associated gene detected. Different correlations of the virulence-associated genes with O serotypes were also investigated and 50% 078 isolates had a gene distribution patterns of fimC^+iucD^+irp2^+fyuA^+iss^+colV^+tsh^+.展开更多
The tools available for genome engineering have significantly improved over the last 5 years, allowing scientist to make precise edits to the genome. Along with the development of these new genome editing tools has co...The tools available for genome engineering have significantly improved over the last 5 years, allowing scientist to make precise edits to the genome. Along with the development of these new genome editing tools has come advancements in technologies used to deliver them. In mammals genome engineering tools are typically delivered into in vitro fertilized single cell embryos which are subsequently cultured and then implanted into a recipient animal.In avian species this is not possible, so other methods have been developed for genome engineering in birds. The most common involves in vitro culturing of primordial germ cells(PGCs), which are cells that migrate through the embryonic circulatory system to the developing gonad and colonize the gonad, eventually differentiating into the gonadocytes which produce either sperm or ova. While in culture the PGCs can be modified to carry novel transgenes or gene edits, the population can be screened and enriched, and then transferred into a recipient embryo. The largest drawback of PGC culture is that culture methods do not transfer well across avian species, thus there are reliable culture methods for only a few species including the chicken. Two newer technologies that appear to be more easily adapted in a wider range of avian species are direct injection and sperm transfection assisted gene editing(STAGE).The direct injection method involves injecting genome engineering tools into the circulatory system of the developing embryo just prior to the developmental time point when the PGCs are migrating to the gonads. The genome engineering tools are complexed with transfection reagents, allowing for in vivo transfection of the PGCs. STAGE utilizes sperm transfection to deliver genome engineering tools directly to the newly fertilized embryo. Preliminary evidence indicates that both methodologies have the potential to be adapted for use in birds species other than the chicken, however further work is needed in this area.展开更多
In recent years,the avian influenza has brought not only serious economic loss to the poultry industry in China but also a serious threat to human health because of the avian influenza virus(AIV) gene recombination an...In recent years,the avian influenza has brought not only serious economic loss to the poultry industry in China but also a serious threat to human health because of the avian influenza virus(AIV) gene recombination and reassortment.Until now,traditional RT-PCR,fluorescence RT-PCR and virus isolation identification have been developed and utilized to detect AIV,but these methods require high-level instruments and experimental conditions,not suitable for the rapid detection in field and farms.In order to develop a rapid,sensitive and practical method to detect and identify AIV subtypes,4 specific primers to the conserved region of AIV M gene were designed and a loop-mediated isothermal amplification(RT-LAMP) method was established.Using this method,the M gene of H1–H16 subtypes of AIV were amplified in 30 min with a water bath and all 16 H subtypes of AIV were able to be visually identified in presence of fluorescein,without cross reaction with other susceptible avian viruses.In addition,the detection limit of the common H1,H5,H7,and H9 AIV subtypes with the RT-LAMP method was 0.1 PFU(plaque-forming unit),which was 10 times more sensitive than that using the routine RT-PCR.Further comparative tests found that the positivity rate of RT-LAMP on detecting clinical samples was 4.18%(14/335) comparing with 3.58%(12/335) from real-time RT-PCR.All these results suggested that the RT-LAMP method can specifically detect and identify AIV with high sensitivity and can be considered as a fast,convenient and practical method for the clinic test and epidemiological investigation of AIV.展开更多
Visual attention is a mechanism that enables the visual system to detect potentially important objects in complex environment. Most computational visual attention models are designed with inspirations from mammalian v...Visual attention is a mechanism that enables the visual system to detect potentially important objects in complex environment. Most computational visual attention models are designed with inspirations from mammalian visual systems.However, electrophysiological and behavioral evidences indicate that avian species are animals with high visual capability that can process complex information accurately in real time. Therefore,the visual system of the avian species, especially the nuclei related to the visual attention mechanism, are investigated in this paper. Afterwards, a hierarchical visual attention model is proposed for saliency detection. The optic tectum neuron responses are computed and the self-information is used to compute primary saliency maps in the first hierarchy. The "winner-takeall" network in the tecto-isthmal projection is simulated and final saliency maps are estimated with the regularized random walks ranking in the second hierarchy. Comparison results verify that the proposed model, which can define the focus of attention accurately, outperforms several state-of-the-art models.This study provides insights into the relationship between the visual attention mechanism and the avian visual pathways. The computational visual attention model may reveal the underlying neural mechanism of the nuclei for biological visual attention.展开更多
A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate (A/Chicken/ Hebei/WD/98, abbreviated as WD98) by comparing with other reference strains. I...A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate (A/Chicken/ Hebei/WD/98, abbreviated as WD98) by comparing with other reference strains. I-Method3 Eight complete genes were amplified by RT-PCR and sequenced. The homology and genetic evolution relationship were analyzed between these sequences and that of the seven reference strains. [Result] The whole genomic sequence of WD98 strain was 91.1% -95.8% homologous to that of seven reference strains tested. This isolate shared the highest homology (95.8%) to D/HK/Y280/97 and the lowest homology (91.1% ) to C/Pak/2/99. The HA cleavage site of the WD98 strain was R-S-S-R G, and the 226th amino acid at receptor-binding site was Gin. [ Condmion] WD98 strain belongs to mildly pathogenic avian in- fluenza virus and may not infect human. The genetic relationship is the closest between A/Chicken/Hebei/wD/98 and A/duck/HongKong/Y280/ 97, both of which belong to the sub-line of A/Chicken/Beijing/1/94 in Eurasian line. And A/Chicken/Hebei/WD/98 and A/Chicken/Beijing/1/94 are genetically distant within the same sub-line.展开更多
文摘One caveat to the dinosaur’s extinction is the conclusion that avian dinosaurs survived and became ancestors of birds. Their mobility enabled them to migrate great distances and find the nutrients needed to survive. Given this scenario, could the current observable migration of birds (the “dinosaurian offspring”) now be related? Migration is the regular seasonal movement undertaken by many species of birds, with the most common pattern, flying north in the Northern spring to breed in the temperate or Arctic summer and returning in the Northern autumn to wintering grounds in warmer regions of the south. The primary motivation for migration appears to be food. None of the major North-South migratory pathways fly over the Caribbean but three main fly ways, past to the west of the theorized K-T impact centre. Due to their ability to fly, the “avian Dinosaurs” adapted and survived very quickly in response to the disaster that marked the K-T boundary. It is an interesting speculation that the avian migration that we witness today is rooted in an event that occurred 66 million years ago! But it does explain why the migratory birds mostly fly from Polar summer to polar summer when they could just be as easily fly from Polar zone to the warmer equatorial region and back. In the recent article in Nature by Melanie During about identifying the late spring timing of the “Astro disaster”, it can be cited as consistent with my speculation. A late April early May Impact as suggested by During would have seen these migrations completely. The western migratory routes would have been found to be “luxurious” in vegetation in that first northern autumn after the “Astro-impact” while all eastern routes would have still been barren.
基金This work was supported by the National Key Research andDevelopment Programof China(2021YFD1800200 and2021YFC2301700).
文摘The stamping-out strategy has been used to control highly pathogenic avian influenza viruses in many countries,driven by the belief that vaccination would not be successful against such viruses and fears that avian influenza virus in vaccinated birds would evolve more rapidly and pose a greater risk to humans.In this review,we summarize the successes in controlling highly pathogenic avian influenza in China and make suggestions regarding the requirements for vaccine selection and effectiveness.In addition,we present evidence that vaccination of poultry not only eliminates human infection with avian influenza virus,but also significantly reduces and abolishes some harmful characteristics of avian influenza virus.
基金supported by the National Key Research and Development Program of China (2022YFD1800604)the China Agricultural Research System (CARS-41)the Heilongjiang Touyan Innovation Team Program of China
文摘Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.
基金supported by the earmarked fund for China Agriculture Research System(CARS-40)the Key Research and Development Project of Yangzhou(Modern Agriculture),China(YZ2022052)the‘‘High-end Talent Support Program’’of Yangzhou University,China。
文摘H7N9 subtype avian influenza virus poses a great challenge for poultry industry.Newcastle disease virus(NDV)-vectored H7N9 avian influenza vaccines(NDV_(vec)H7N9)are effective in disease control because they are protective and allow mass administration.Of note,these vaccines elicit undetectable H7N9-specific hemagglutination-inhibition(HI)but high IgG antibodies in chickens.However,the molecular basis and protective mechanism underlying this particular antibody immunity remain unclear.Herein,immunization with an NDV_(vec)H7N9 induced low anti-H7N9 HI and virus neutralization titers but high levels of hemagglutinin(HA)-binding IgG antibodies in chickens.Three residues(S150,G151 and S152)in HA of H7N9 virus were identified as the dominant epitopes recognized by the NDV_(vec)H7N9 immune serum.Passively transferred NDV_(vec)H7N9 immune serum conferred complete protection against H7N9 virus infection in chickens.The NDV_(vec)H7N9 immune serum can mediate a potent lysis of HA-expressing and H7N9 virus-infected cells and significantly suppress H7N9 virus infectivity.These activities of the serum were significantly impaired after heat-inactivation or treatment with complement inhibitor,suggesting the engagement of the complement system.Moreover,mutations in the 150-SGS-152 sites in HA resulted in significant reductions in cell lysis and virus neutralization mediated by the NDV_(vec)H7N9 immune serum,indicating the requirement of antibody-antigen binding for complement activity.Therefore,antibodies induced by the NDV_(vec)H7N9 can activate antibody-dependent complement-mediated lysis of H7N9 virus-infected cells and complement-mediated neutralization of H7N9 virus.Our findings unveiled a novel role of the complement in protection conferred by the NDV_(vec)H7N9,highlighting a potential benefit of engaging the complement system in H7N9 vaccine design.
基金supported by the National Natural Science Foundation of China Young Scholars Project(31902242)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences(2017–2020)。
文摘Avian pathogenic Escherichia coli(APEC)belonging to extraintestinal pathogenic E.coli(ExPEC)can cause severe infections in extraintestinal tissues in birds and humans,such as the lungs and blood.MprA(microcin production regulation,locus A,herein renamed AbsR,a blood survival regulator),a member of the MarR(multiple antibiotic resistance regulator)transcriptional regulator family,governs the expression of capsule biosynthetic genes in human ExPEC and represents a promising druggable target for antimicrobials.However,a deep understanding of the AbsR regulatory mechanism as well as its regulon is lacking.In this study,we present a systems-level analysis of the APEC AbsR regulon using ChIP-Seq(chromatin immunoprecipitation sequencing)and RNA-Seq(RNA sequencing)methods.We found that AbsR directly regulates 99 genes and indirectly regulates 667 genes.Furthermore,we showed that:1)AbsR contributes to antiphagocytotic effects by macrophages and virulence in a mouse model for systemic infection by directly activating the capsular gene cluster;2)AbsR positively impacts biofilm formation via direct regulation of the T2SS(type II secretion system)but plays a marginal role in virulence;and 3)AbsR directly upregulates the acid tolerance signaling system EvgAS to withstand acid stress but is dispensable in ExPEC virulence.Finally,our data indicate that the role of AbsR in virulence gene regulation is relatively conserved in ExPEC strains.Altogether,this study provides a comprehensive analysis of the AbsR regulon and regulatory mechanism,and our data suggest that AbsR likely influences virulence primarily through the control of capsule production.Interestingly,we found that AbsR severely represses the expression of the type I-F CRISPR(clustered regularly interspaced short palindromic repeats)-Cas(CRISPR associated)systems,which could have implications in CRISPR biology and application.
基金supported by the National Natural Science Foundation of China(NSFC,31971413)granted to DLthe Second Tibetan Plateau Scientific Expedition and Research Program(STEP,2019QZKK0501)granted to DL。
文摘Avian embryos,which develop within eggs,exhibit remarkable tolerance to extremely low temperatures.Despite being a common trait among all birds,the mechanisms underlying this cold tolerance in avian embryos remain largely unknown.To gain a better understanding of this phenomenon and the coping mechanisms involved,we reviewed the literature on severe cold tolerance in embryos of both wild and domestic birds.We found that embryos of different bird orders exhibit tolerance to severe cold during their development.In response to cold stress,embryos slow down their heartbeat rates and metabolism.In severe cold temperatures,embryos can suspend these processes,entering a torpid-like state of cardiac arrest.To compensate for these developmental delays,embryos extend their regular incubation periods.Depending on their embryonic age,embryos of all bird species can tolerate acute severe cold regimes;only a few tolerate chronic severe cold regimes.We also discussed various extrinsic and intrinsic factors that affect the tolerance of bird embryos to low temperatures before and after incubation.Cold tolerance appears to be a heritable trait shared by wild and domestic embryos of all bird classes,regardless of egg size or development(altricial/precocial).Driven by environmental variability,cold tolerance in avian embryos is an optimal physiological and ecological strategy to mitigate the adverse effects of cold conditions on their development in response to fluctuating environmental temperatures.
基金supported by the National Key R&D Program of China(2022YFF1000100)National Waterfowl-Industry Technology Research System(CARS-42–09)+1 种基金National Nature Science Foundation of China(31972525,31572388)Beijing Joint Research Program for Germplasm Innovation and New Variety Breeding(G20220628007).
文摘Background The fat deposition has a crucial role in animal meat flavor,and fat deposition-related traits are vital for breeding in the commercial duck industry.Avian fat-related traits are typical complex phenotypes,which need a large amount of data to analyze the genetic loci.Results In this study,we performed a new phenotypic analysis of fat traits and genotyped whole-genome varia-tions for 1,246 ducks,and combed with previous GWAS data to reach 1,880 ducks for following analysis.The carcass composition traits,subcutaneous fat weight(SFW),subcutaneous fat percentage(SFP),abdominal fat weight(AFW),abdominal fat percentage(AFP)and the body weight of day 42(BW42)for each duck were collected.We identified a set of new loci that affect the traits related to fat deposition in avian.Among these loci,ceroid-lipofuscinosis,neuronal 8(CLN8)is a novel candidate gene controlling fat deposition.We investigated its novel function and regulation in avian adipogenesis.Five significant SNPs(the most significant SNP,P-value=21.37E-12)and a single haplotype were detected in the upstream of CLN8 for subcutaneous fat percentage.Subsequently,luciferase assay demonstrated that 5 linked SNPs in the upstream of the CLN8 gene significantly decreased the transcriptional activity of CLN8.Further,ATAC-seq analysis showed that transcription factor binding sites were identified in a region close to the haplotype.A set of luciferase reporter gene vectors that contained different deletion fragments of the CLN8 promoter were con-structed,and the core promoter area of CLN8 was finally identified in the-1,884/-1,207 bp region of the 5′flanking sequences,which contains adipogenesis-related transcription factors binding sites.Moreover,the over-expression of CLN8 can remarkably facilitate adipocyte differentiation in ICPs.Consistent with these,the global transcriptome profiling and functional analysis of the over-expressed CLN8 in the cell line further revealed that the lipid biosynthetic process during the adipogenesis was significantly enriched.Conclusions Our results demonstrated that CLN8 is a positive regulator of avian adipocyte differentiation.These findings identify a novel function of CLN8 in adipocyte differentiation,which provides important clues for the further study of the mechanism of avian fat deposition.
文摘Recent studies have revealed concerning declines in bird populations in Europe and other parts of the world.In understudied but biodiverse regions,especially those that are located along key migratory flyways,there is an unmet need to evaluate the status of resident and migratory birdlife for avian conservation ecology.This is especially urgent at a time when recent regional studies have highlighted the scale of the decline of the European and Palearctic avifauna.Rapidly growing community science datasets may help bridge the gaps in our understanding of long-term regional avifaunal trends.In this study,we used eBird data submitted by observers in Türkiye to undertake the first long-term assessment of the state of the country’s birds from 2001 to 2022.We evaluated population trends based on presence-absence data of species to determine if any birds have significantly increased or decreased in the country so far this century.We provide a list of trends for 418 bird species that had large enough sample sizes of eBird observations from Türkiye,and highlight which species have significantly declined or increased.We found that 63 species have significantly declined in detection while 120 have significantly increased during the study period.Our findings provide a baseline for the future monitoring of Türkiye’s birds and help contextualize population changes of birds in Türkiye against those occurring at the continental(Palearctic)scale.Additionally,we include movement classifications for all of the bird species in this study.We also provide a framework to effectively use eBird data to assess long-term avifaunal changes at the country-level in other understudied regions.Finally,we highlight ways in which eBirders in Türkiye and elsewhere can strengthen the quality and value of community ornithology data for population assessments and avian conservation ecology.
基金support provided by Facility of Bioscience Applied Techniques of SAIUEx(financed by UEX,Junta de Extremadura,MICINN,FEDER and FSE)funded by Consejería de Economía e Infraestructura of the Junta de Extremadura and the European Regional Development Fund,a Way to Make Europe(research projects IB16121 and IB20089)+1 种基金supported by a postdoctoral grant from the Juan de la Cierva Subprogram(FJCI 2017-34109,MICINN)a postdoctoral contract for scientific excellence in the development of the Plan Propio de I+D+i of the UCLM(co-funded by the European Social Fund Plus(ESF+))。
文摘Avian malaria and related haemosporidian parasites can negatively impact fitness in many songbirds.Research on the malaria infection and its physiological costs on their avian hosts is heavily skewed toward native passerines,with exotic species underrepresented.However,introduced species may carry on and spread new pathogens to native species,and play a role on parasite transmission cycle in invaded bird communities as pathogen reservoir.Here,we molecularly assess the prevalence and diversity of haemosporidian parasites in three introduced wetland passerines(the Red Avadavat Amandava amandava,the Yellow-crowned Bishop Euplectes afer,and the Common Waxbill Estrilda astrild)captured during the same season in southwestern Spain.We also explored the relation between parasite infection,body condition,haematocrit,and uropygial gland volume.We detected an overall parasite prevalence of 3.55%,where Common Waxbills showed higher prevalence(6.94%)than Red Avadavats(1.51%).None Yellow-crowned Bishops were infected with haemosporidians.Almost 60%of infections were caused by Leucocytozoon,and about 40%by Plasmodium.We identified four unique lineages of Plasmodium and three of Leucocytozoon.Moreover,91%of the identified host-parasite interactions represented new host records for these haemosporidian parasites.Parasite infection was not related to body condition,haematocrit,and uropygial gland volume of the wetland passerines.Haematocrit values varied seasonally among bird species.Additionally,haematocrit was positively related to body condition in the Yellow-crowned Bishops,but not in the other species.Red Avadavats had higher haematocrit levels than Yellow-crowned Bishops,whereas Common Waxbills showed the lower haematocrit values.The uropygial gland volume was positively correlated with body condition in all bird species.Common Waxbills showed higher uropygial gland volumes related to their body size than birds from other two species.These outcomes highlight the importance of exotic invasive species in the transmission dynamics of haemosporidian parasites.
文摘BACKGROUND Most of the first symptoms of avian influenza are respiratory symptoms,and cases with occipital neuralgia as the first manifestation are rarely reported.CASE SUMMARY A middle-aged patient complaining of paroxysmal pain behind the ear was admitted to our hospital.The patient’s condition changed rapidly,and high fever,unexpected respiratory failure,and multiple organ failure developed rapidly.The patient was diagnosed with H7N9 avian influenza based on etiology.CONCLUSION We believe that the etiology of occipital neuralgia is complex and could be the earliest manifestation of severe diseases.The possibility of an infectious disease should be considered when occipital neuralgia is accompanied by fever.Avian influenza is one of these causative agents.
文摘Among the avian species, understanding the roles of activin happen to be a dominant challenge in genetic evolution due to its complexity in nature. A case study of the activin gene in avian species was carried out using bioinformatics. As a sedentary bird, guinea fowl is more susceptible to local selection processes and needs a proper genetic study for conservation. The present study provides the basis for the use of activin or its target genes for the improvement of impaired wound healing, and activin antagonists for the prevention and treatment of fibrosis and the end of malignant tumors that over-express activin. The information provided will serve as a basic tool for broader genetic diversity studies to identify valuable poultry genetic resources and major genes for the development of breeding programs. This study was done by retrieving hundred (100) nucleotides and amino acid sequences of the activin gene belonging to guinea fowl and other avians from the GeneBank, aligning the sequences using BlastP determined the percent identity and phylogenetic relationship of the activin gene of guinea fowl and other avians. The shortest activin nucleotide sequence (467 bp) was observed in chicken and the longest (39896445 bp) in duck. Using the comparative sequence analysis, it was observed that the activin gene of chickens, turkeys and guinea fowl shared percent identity ranging from 91% to 95%. The percent identity reflects the degree of relatedness of species. Although closely related (90%) in ancestral line, the activin gene of guinea fowl and quail cannot be compared with guinea fowl-turkey (95%) nor guinea fowl-chicken (90%), in both biological functions and evolutionary relationship. Finally, the percent identity and similarity in function of the activin gene of guinea fowl, turkey, and chicken were in the range of 93% - 100%, indicating that the activin gene of avians possesses similar functions, well conserved and is very effective in performing functions like increasing FSH bindings, FSH-induced aromatization, improves wound healing and enhances scar formation, regulates morphogenesis of branching organs, and enhances ovarian folliculogenesis. The study, therefore, recommends farmers select and breed for activin genes in order to promote reproductive efficiency, thereby barricading species extinction.
文摘Global climate change affects many facets of avian ecology, such as shifts in breeding phenology and migration patterns. Migrating bird species respond to changes in climate by shifting their temporal patterns of spring migration. However, variation in species’ responses exists based on various life history traits, which exposes some species to an increased risk of phenological mismatch. This study examined the spring arrival dates of 115 migrating species over 127 years (1889-2015) using archival sources in West Virginia, USA, making this research unique in the length of study, the high number of species studied, and the historical crowd-sourced observations analyzed. Of the 115 taxa, 45 showed significant negative slopes of spring arrival dates (arriving earlier in the spring) plotted against the year. In contrast, only nine species showed positive slopes (arriving later in the spring), albeit non-significant. The average advance of spring arrival date for all species was 1.7 days per decade, and an advance of 2.6 days per decade in species that showed significance. Arrival dates were associated with increasing spring temperatures—for each 1˚C increase, the arrival date advanced by 0.81 days/decade. Several life history traits were linked to species that advanced their first arrival dates, including a shorter distance migrated to reach wintering grounds, increasing populations, and foraging habitat. Most avian species are advancing their spring arrival dates in response to climate change. However, the implications of earlier spring arrival are unclear. We draw attention to shifts in arrival dates and wintering ranges, leading to a possible increase in overwintering in the mid-latitudes of North America.
基金supported by funds provided by South China Agricultural University and Guangzhou work team project(No 2011A020102009)
文摘This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an Mqq- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MFIC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV wet enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on HgN2 infection was evaluated by an M]q- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to PIgN2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces HgN2 AIV replication and promotes early humoral immune responses in young chickens.
基金supported by the Jiangsu Province Health Development Project with Science and Education (No.ZX201109 and RC2011085)the Research Projects of Jiangsu Preventive Medicine (No.YZ201020)
文摘A novel avian influenza A (H7N9) virus was discovered in February 2013 in China and has resulted in more than 100 comfirmed human infections including 26 fatal cases as of May 2, 2013. The situation raises many ur- gent questions and global public health concerns. In this study, epidemiologic characteristics of infected human cases in Jiangsu province were analyzed and risk assessment was undertaken based on the information available. Briefly, it is highly unlikely that a pandemic of human infection with avian influenza A (HTN9) virus will happen in Jiangsu Province in the near future. Iia the end, some measures are recommended to prevent the situation from becoming worse.
基金the National Natural Science Foundation of China (30800822)Jiangsu Prov-ince Natural Science Foundation (BK2006070)Jiangsu Education Department (VK0410190)
文摘216 avian pathogenic Escherichia coli (APEC) isolates were obtained from poultry with colibacillosis in different areas of China. Among them, 195 were serotyped as 078, 088, and 093. Thirteen virulence-associated genes, including fimC, iucD, iss, tsh, fyuA, irp2, eaeA, hlyE, colV, papC, stx2f, vat, and astA, were submitted to PCR amplification. The fimC gene was the most prevalent with a detection rate of 93.6%, followed by iucD (70.8%), iss (58.8%), and tsh (51.4%) in APEC isolates. The detection rate of high pathogenicity islands (HPI)-associatedfyuA and irp2 genes were both 44.9%, with no LEE (the locus of enterocyte effacement) island-associated gene eaeA detected. In terms of distribution patterns of the 13 virulence-associated genes, 5 isolates harborbed 10 genes, 19 isolates contained onlyfimC gene, and only 4 isolates had no virulence-associated gene detected. Different correlations of the virulence-associated genes with O serotypes were also investigated and 50% 078 isolates had a gene distribution patterns of fimC^+iucD^+irp2^+fyuA^+iss^+colV^+tsh^+.
文摘The tools available for genome engineering have significantly improved over the last 5 years, allowing scientist to make precise edits to the genome. Along with the development of these new genome editing tools has come advancements in technologies used to deliver them. In mammals genome engineering tools are typically delivered into in vitro fertilized single cell embryos which are subsequently cultured and then implanted into a recipient animal.In avian species this is not possible, so other methods have been developed for genome engineering in birds. The most common involves in vitro culturing of primordial germ cells(PGCs), which are cells that migrate through the embryonic circulatory system to the developing gonad and colonize the gonad, eventually differentiating into the gonadocytes which produce either sperm or ova. While in culture the PGCs can be modified to carry novel transgenes or gene edits, the population can be screened and enriched, and then transferred into a recipient embryo. The largest drawback of PGC culture is that culture methods do not transfer well across avian species, thus there are reliable culture methods for only a few species including the chicken. Two newer technologies that appear to be more easily adapted in a wider range of avian species are direct injection and sperm transfection assisted gene editing(STAGE).The direct injection method involves injecting genome engineering tools into the circulatory system of the developing embryo just prior to the developmental time point when the PGCs are migrating to the gonads. The genome engineering tools are complexed with transfection reagents, allowing for in vivo transfection of the PGCs. STAGE utilizes sperm transfection to deliver genome engineering tools directly to the newly fertilized embryo. Preliminary evidence indicates that both methodologies have the potential to be adapted for use in birds species other than the chicken, however further work is needed in this area.
基金supported by the Special Foundation for State Basic Research Program of China(2013FY113300-8)the National Key R&D Program of China(2016YFD0500800)
文摘In recent years,the avian influenza has brought not only serious economic loss to the poultry industry in China but also a serious threat to human health because of the avian influenza virus(AIV) gene recombination and reassortment.Until now,traditional RT-PCR,fluorescence RT-PCR and virus isolation identification have been developed and utilized to detect AIV,but these methods require high-level instruments and experimental conditions,not suitable for the rapid detection in field and farms.In order to develop a rapid,sensitive and practical method to detect and identify AIV subtypes,4 specific primers to the conserved region of AIV M gene were designed and a loop-mediated isothermal amplification(RT-LAMP) method was established.Using this method,the M gene of H1–H16 subtypes of AIV were amplified in 30 min with a water bath and all 16 H subtypes of AIV were able to be visually identified in presence of fluorescein,without cross reaction with other susceptible avian viruses.In addition,the detection limit of the common H1,H5,H7,and H9 AIV subtypes with the RT-LAMP method was 0.1 PFU(plaque-forming unit),which was 10 times more sensitive than that using the routine RT-PCR.Further comparative tests found that the positivity rate of RT-LAMP on detecting clinical samples was 4.18%(14/335) comparing with 3.58%(12/335) from real-time RT-PCR.All these results suggested that the RT-LAMP method can specifically detect and identify AIV with high sensitivity and can be considered as a fast,convenient and practical method for the clinic test and epidemiological investigation of AIV.
基金supported by Natural Science Foundation of China(61425008,61333004,61273054)
文摘Visual attention is a mechanism that enables the visual system to detect potentially important objects in complex environment. Most computational visual attention models are designed with inspirations from mammalian visual systems.However, electrophysiological and behavioral evidences indicate that avian species are animals with high visual capability that can process complex information accurately in real time. Therefore,the visual system of the avian species, especially the nuclei related to the visual attention mechanism, are investigated in this paper. Afterwards, a hierarchical visual attention model is proposed for saliency detection. The optic tectum neuron responses are computed and the self-information is used to compute primary saliency maps in the first hierarchy. The "winner-takeall" network in the tecto-isthmal projection is simulated and final saliency maps are estimated with the regularized random walks ranking in the second hierarchy. Comparison results verify that the proposed model, which can define the focus of attention accurately, outperforms several state-of-the-art models.This study provides insights into the relationship between the visual attention mechanism and the avian visual pathways. The computational visual attention model may reveal the underlying neural mechanism of the nuclei for biological visual attention.
基金supported by subproject of National Program on Key Basic Research Project (973 Program )(2005CB523001)
文摘A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate (A/Chicken/ Hebei/WD/98, abbreviated as WD98) by comparing with other reference strains. I-Method3 Eight complete genes were amplified by RT-PCR and sequenced. The homology and genetic evolution relationship were analyzed between these sequences and that of the seven reference strains. [Result] The whole genomic sequence of WD98 strain was 91.1% -95.8% homologous to that of seven reference strains tested. This isolate shared the highest homology (95.8%) to D/HK/Y280/97 and the lowest homology (91.1% ) to C/Pak/2/99. The HA cleavage site of the WD98 strain was R-S-S-R G, and the 226th amino acid at receptor-binding site was Gin. [ Condmion] WD98 strain belongs to mildly pathogenic avian in- fluenza virus and may not infect human. The genetic relationship is the closest between A/Chicken/Hebei/wD/98 and A/duck/HongKong/Y280/ 97, both of which belong to the sub-line of A/Chicken/Beijing/1/94 in Eurasian line. And A/Chicken/Hebei/WD/98 and A/Chicken/Beijing/1/94 are genetically distant within the same sub-line.