[ Objective] To prepare inactivated emulsion vaccine against Newcastle disease, infectious bronchitis and H9 subtype avian influenza. [ Method] Antigen fluid of Newcastle disease virus （NDV） La Sota strain, infectio...[ Objective] To prepare inactivated emulsion vaccine against Newcastle disease, infectious bronchitis and H9 subtype avian influenza. [ Method] Antigen fluid of Newcastle disease virus （NDV） La Sota strain, infectious bronchitis virus （IBV） M41 strain and HgN2 subtype avian in- fluenza virus （AIV） WD strain was prepared by propagation in chicken embryos, respectively. The antigen fluid was concentrated with FILTRON Cassette ultra-filtration system and inactivated by formalin. The antigen fluid of NDV, IBV and AIV was mixed at a volume ratio of 1：1：1. Then the mixture was emulsified by Span-80 and Tween-80 and added medical white oil as adjuvant. The sterility and physical characteristics of the prepared ND-IB-AI combined vaccine were detected. [ Result] The three batches of ND-IB-AI combined vaccine were germ-free, milky white, with water-in- oil pattern and with viscosity of 6.3 -6.8 s. The water and oil were not separated after rest at 37 ~C for 21 d or centrifugation. [ Conclusion] The three batches of ND-IB-AI combined vaccine were germ-free and reached the standard for physical characteristics of vaccines.展开更多
The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacte...The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacterium paragal-linarum occurred in the Qinling area of China,resulting in clinical symptoms of facial swelling in several bird species,including Golden pheasant,Temminck's tragopan,and Peafowls,and three Golden pheasants died due to prolonged infection.Specific PCR results confrmed the presence of the pathogen in the infected birds.The report describes the clinical symptoms and pathological changes observed in the affected birds,as well as the isolation and identification of Avibacterium paragallinarum.Whole-genome sequencing and phylogenetic anal sis y were performed,and this is the frst report of inter-and intra-species transmission of infectious coryza among wild birds in China.展开更多
文摘[ Objective] To prepare inactivated emulsion vaccine against Newcastle disease, infectious bronchitis and H9 subtype avian influenza. [ Method] Antigen fluid of Newcastle disease virus （NDV） La Sota strain, infectious bronchitis virus （IBV） M41 strain and HgN2 subtype avian in- fluenza virus （AIV） WD strain was prepared by propagation in chicken embryos, respectively. The antigen fluid was concentrated with FILTRON Cassette ultra-filtration system and inactivated by formalin. The antigen fluid of NDV, IBV and AIV was mixed at a volume ratio of 1：1：1. Then the mixture was emulsified by Span-80 and Tween-80 and added medical white oil as adjuvant. The sterility and physical characteristics of the prepared ND-IB-AI combined vaccine were detected. [ Result] The three batches of ND-IB-AI combined vaccine were germ-free, milky white, with water-in- oil pattern and with viscosity of 6.3 -6.8 s. The water and oil were not separated after rest at 37 ~C for 21 d or centrifugation. [ Conclusion] The three batches of ND-IB-AI combined vaccine were germ-free and reached the standard for physical characteristics of vaccines.
基金This study has been supported by Shaanxi Special Fund Project of Giant Panda International Cooperation(Panda Protection Letter[2019]No.93)the Project of Course Case Library Construction(Grant No.Z1050221011).
文摘The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacterium paragal-linarum occurred in the Qinling area of China,resulting in clinical symptoms of facial swelling in several bird species,including Golden pheasant,Temminck's tragopan,and Peafowls,and three Golden pheasants died due to prolonged infection.Specific PCR results confrmed the presence of the pathogen in the infected birds.The report describes the clinical symptoms and pathological changes observed in the affected birds,as well as the isolation and identification of Avibacterium paragallinarum.Whole-genome sequencing and phylogenetic anal sis y were performed,and this is the frst report of inter-and intra-species transmission of infectious coryza among wild birds in China.
