Marine spatial planning(MSP)is designed to divide the sea area into different types of functional zones,to implement corresponding development activities.However,the long-term impacts of anthropogenic activities assoc...Marine spatial planning(MSP)is designed to divide the sea area into different types of functional zones,to implement corresponding development activities.However,the long-term impacts of anthropogenic activities associated with MSP practice on the marine microbial biosphere are still unclear.Yalu River Estuary,a coastal region in northeast of China,has been divided into fishery&agricultural(F&A)zone,shipping&port(S&P)zone and marine protected area(MPA)zone by a local MSP guideline that has been run for decades.To examine the effects of long-term executed MSP,benthic bacterial communities from different MSP zones were obtained and compared in this study.The results revealed significant differences in the bacterial community structure and predict functions among different zones.Bacterial genera enriched in different zones were identified,including SBR1031 in MPA,Woeseia and Sva0996 in S&P,and Halioglobus in F&A.In addition,correlations between some bacterial genera and sediment pollutants were uncovered.Furthermore,bacteria related to sulphide production were more abundant in the F&A zone,which was according to the accumulation of sulphides in this area.Moreover,bacteria associated with chemoheterotrophy and fermentation were more predominant in the S&P zone,consistent with high levels of organic matter and petroleum caused by shipping.Our findings indicated benthic bacterial communities could bring to light the anthropogenic activity footprints by different activities induced by long-term MSP practice.展开更多
Sexual dimorphism of plants shapes the diff erent morphology and physiology between males and females.However,it is still unclear whether it infl uences belowground ecological processes.In this study,rhizosphere soil ...Sexual dimorphism of plants shapes the diff erent morphology and physiology between males and females.However,it is still unclear whether it infl uences belowground ecological processes.In this study,rhizosphere soil of male and female Populus deltoides and bulk soil were collected from an 18-year plantation(male and female trees mix-planted)and grouped into three soil compartments.Soil carbon(C),nitrogen(N)and phosphorus(P)levels were determined,and soil bacterial communities were analyzed by high-throughput sequencing.The results showed the less total carbon and total organic carbon,the more nutrients(available phosphorus,nitrate nitrogen and ammonium nitrogen)available in the rhizosphere soils of female poplars than soils of males.However,α-diversity indices of the rhizosphere bacterial communities under male plants were signifi-cantly higher.Principal component analysis showed that the bacterial communities were signifi cantly diff erent between the male and female soil compartments.Further,the bacterial co-occurrence network in soil under male trees had more nodes and edges than under females.BugBase analysis showed the more functional bacteria taxa related to biofi lm formation and antioxidation under males.The results indicate that soils under male poplars had more diverse and more complex co-occurrence networks of the rhizosphere bacterial community than soils under female trees,implying that male poplars might have better environmental adaptability.The study provides insight into the diff erent soil-microbe interactions of dioecious plants.More details about the infl uencing mechanism of sexual dimorphism on rhizosphere soil bacterial communities need to be further studied.展开更多
This study aimed to investigate bacterial community in an urban drinking water distribution system (DWDS) during an occurrence of colored water. Variation in the bacterial community diversity and structure was obser...This study aimed to investigate bacterial community in an urban drinking water distribution system (DWDS) during an occurrence of colored water. Variation in the bacterial community diversity and structure was observed among the different waters, with the predominance of Proteobacteria. While Verrucomicrobia was also a major phylum group in colored water. Limnobacter was the major genus group in colored water, but Undibacterium predominated in normal tap water. The coexistence of Limnobacter as well as Sediminibacterium and Aquobocterium might contribute to the formation of colored water.展开更多
The Changjiang River Estuary(CRE)in the East China Sea suffers from seasonal hypoxia in summer.The vertical distributions and seasonal changes of microbial communities in the CRE were well documented.However,little is...The Changjiang River Estuary(CRE)in the East China Sea suffers from seasonal hypoxia in summer.The vertical distributions and seasonal changes of microbial communities in the CRE were well documented.However,little is known about the diurnal changes of bacterial communities in the hypoxic zone of the CRE.Here,16 S rRNA gene analysis was used to explore the changes of bacterial communities in the oxic surface and hypoxic middle seawater layers during 24 h in the CRE.Significant differences between the hypoxic and oxic layers were observed:the phyla Cyanobacteria,Bacteroidetes and Acidimicrobiia were enriched in the oxic layer,whereas the phylum SAR406 and the class Deltaproteobacteria were more abundant in the hypoxic layer.In addition,some subtle diurnal variations of the bacterial relative abundance were found in both two layers.The relative abundance of Synechococcus increased at night,and this change was more obvious in the hypoxic layer.The similar trend was also found in some phototrophic and several heterotrophic bacteria,such as Rhodobacteraceae,OM60 and Flavobacteriaceae.Their relative abundances peaked at 16:00 in the oxic layer,while the relative abundances peaked at around 7:00 and decreased until 13:00 in the hypoxic layer.Together,the results of the present study suggest that some photosynthetic bacteria and several heterotrophic bacteria have similar diurnal variations implying the light and physicochemical heterogeneity in the course of a day are important for bacterial diurnal changes in the CRE.展开更多
Coated controlled-release fertilizers (CRFs) have been widely applied in agriculture due to their increased efficiency. However, the widespread and a lot of coated CRFs application may leave undesired coating residu...Coated controlled-release fertilizers (CRFs) have been widely applied in agriculture due to their increased efficiency. However, the widespread and a lot of coated CRFs application may leave undesired coating residues in the soil due to their slow degradation. Limited information is available on the effects of substantial residual coatings on the soil bacterial community. By adding 0, 5, 10, 20, and 50 times quantities of residual coating from conventional application amount of resin and water-soluble coated CRFs, we studied the responses of soil properties and bacterial community composition to these two residual coatings in black soil. The results showed that the resin and water-soluble coatings did not essentially alter the properties of black soil or cause dramatic changes to bacterial diversity within the test concentration range. The residual resin and water-soluble coatings also did not distinctly alter the relative abundance of the top ten bacteria at phylum level. Heatmap results suggested that the treatments were basically clustered into two groups by concentration rather than types of coating material. Pearson correlation analysis showed that the Simpson's diversity index of the bacterial community was significantly correlated with microbial biomass carbon (MBC, r=0.394, P〈0.05), and the richness index abundance-based coverage estimator (ACE) of the bacterial community was significantly correlated with microbial biomass nitrogen (MBN, t=0.407, P〈0.05). Overall, results of this study suggested that substantial residual resin and water-soluble coatings with 0-50 times quantities of residual coating from conventional application amount of coated CRFs did not generate obviously negative impacts on the bacterial community in black soil.展开更多
Besides being critical components of marine food web,microorganisms play vital roles in biogeochemical cycling of nutrients and elements in the ocean.Currently little is known about microbial population structure and ...Besides being critical components of marine food web,microorganisms play vital roles in biogeochemical cycling of nutrients and elements in the ocean.Currently little is known about microbial population structure and their distributions in the eastern Indian Ocean.In this study,we applied molecular approaches including polymerase chain reaction-denaturant gradient gel electrophoresis(PCR-DGGE) and High-Throughput next generation sequencing to investigate bacterial 16S rRNA genes from the equatorial regions and the adjacent Bay of Bengal in the eastern Indian Ocean.In general,Bacteroidetes,Proteobacteria(mainly Alpha,and Gamma),Actinobacteria,Cyanobacteria and Planctomycetes dominated the microbial communities.Horizontally distinct spatial distribution of major microbial groups was observed from PCR-DGGE gel image analyses.However,further detailed characterization of community structures by pyrosequencing suggested a more pronounced stratified distribution pattern:Cyanobacteria and Actinobacteria were more predominant at surface water(25m);Bacteroidetes dominated at 25m and 150m while Proteobacteria(mainly Alphaproteobacteria) occurred more frequently at 75m water depth.With increasing water depth,the bacterial communities from different locations tended to share high similarity,indicating a niche partitioning for minor groups of bacteria recovered with high throughput sequencing approaches.This study provided the first "snapshot" on biodiversity and spatial distribution of Bacteria in water columns in the eastern Indian Ocean,and the findings further emphasized the potential functional roles of these microbes in energy and resource cycling in the eastern Indian Ocean.展开更多
Available information on the microbial mechanisms associated with heavy metal(HM)passivation during co-composting amended with phosphate rock(PR)remains limited.Thus,this study investigated the dynamic changes in bact...Available information on the microbial mechanisms associated with heavy metal(HM)passivation during co-composting amended with phosphate rock(PR)remains limited.Thus,this study investigated the dynamic changes in bacterial communities and HM-fractions(Zn,Cu,Cd,Cr and Pb)during swine manure composting with maize straw,and ascertained the bacterial influence on HM-passivation.The results demonstrated that the addition of PR improved HM-passivation,especially for Zn and Cd,with their bioavailability factors(BFs)reduced by 247.41 and 176.25%,respectively.As for bacterial communities,the proportion of Firmicutes decreased,while the proportions of Proteobacteria,Bacteroidetes,DeinococcusThermus and Gemmatimonadetes increased in all treatments.PR significantly changed the primary bacterial phyla in the thermophilic phase.Bacteroidetes were the main bacterial component controlling the passivation of Zn,Cu and Cr,while Deinococcus-Thermus mainly regulated the mobility of Zn and Pb,and Proteobacteria only dominated the transformation among Cd-fractions.These results may provide a reference for the use of HM-passivation techniques during composting.展开更多
Bacteria play a major role in metabolizing ammonia and other metabolites in recirculating aquaculture systems(RASs).To characterize and compare the bacterial communities in the biofilters of two full-scale RASs for th...Bacteria play a major role in metabolizing ammonia and other metabolites in recirculating aquaculture systems(RASs).To characterize and compare the bacterial communities in the biofilters of two full-scale RASs for the culture of puffer fish,Takifugu rubripes,at different ages and densities were studied.In overall,47807 optimized reads of the 16 S rRNA gene with V4-V5 region were obtained from four biofilm samples collected after biofilm maturation.At 97%cut-off level,these sequences were clustered into 500 operational taxonomic units,and were classified into 19 bacterial phyla and 138 genera.At the phylum level,Proteobacteria and Bacteroidetes were the most abundant,followed by Nitrospirae and Planctomycetes.At the genus level,Colwellia,Marinifilum,Oceanospirillum,Lutibacter,Winogradskyella,Pseudoalteromonas,Arcobacter,and Phaeobacter were the top members.Nitrosomonas and Nitrospira were main ammonia-and nitrite-oxidizing bacteria.Differences in bacterial communities at different sampling dates and similarities of both biofilters were revealed in the Venn diagram and cluster analysis.Maintaining a good water quality and health offarmed fish in RASs depended on the correct management of the bacterial communities.This study provides more accurate information on the bacterial communities associated with the bifilters of both RASs.展开更多
Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,C...Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.展开更多
Bacteria communities in cnidarian jellyfi sh can be harmful to many important aquaculture species,as they can be key vectors of bacterial pathogens.However,our knowledge of bacterial communities associated with jellyf...Bacteria communities in cnidarian jellyfi sh can be harmful to many important aquaculture species,as they can be key vectors of bacterial pathogens.However,our knowledge of bacterial communities associated with jellyfi sh in culture ponds and their potential roles in the regulation of aquaculture species remains unclear.In this study,sequencing based on the bacterial 16S rRNA gene was used to investigate the composition and variation of the bacterial communities associated with hydromedusa Gonionemus vertens in sea cucumber culture ponds and natural marine environment.The associated bacterial communities of G.vertens from the culture ponds in the Yellow Sea and Bohai Sea had signifi cantly diff erent compositions,when compared with those from ambient seawater environment.Furthermore,bacterial communities associated with G.vertens had similar diversity and composition in culture ponds and natural marine environment in the Yellow Sea.There were 31 unique bacterial biomarkers identifi ed in three locations.The major communities were highly abundant in Kiloniellales,Octadecabacter,Polynucleobacter,and Polaribacter,and are related to the environmental information processing.Pathogen candidates such as Vibrionales and Chlamydiales had notably low relative abundances(<1%).The venom of the jellyfi sh was considered responsible for damage to the aquaculture.This study provides important data to help assess the impact of cnidarians-associated bacterial communities on pond aquaculture and the infl uences on material cycling and energy fl ow in marine ecosystems.展开更多
Over 3 million tons of manures are produced annually in the United States and pose environmental and health risks if not remediated. Anaerobic digestion is an effective method in treating organic wastes to reduce envi...Over 3 million tons of manures are produced annually in the United States and pose environmental and health risks if not remediated. Anaerobic digestion is an effective method in treating organic wastes to reduce environmental impacts and produce methane as an alternative energy. Previous studies suggested that optimization of feed composition, hydraulic retention time, and other operational conditions can greatly improve total solids removal and increase methane productivity. These environmental factors improve functionality by altering the microbial community structure but explicit details of how the bacterial community shifts are poorly understood. Our investigations were conducted to investigate the relationship between environmental factors, microbial community structure and bioreactor efficiency by using metagenomic analysis of the microbial communities. Our results indicated that the bioreactor with the greatest methane production, digestion efficiency and reduced levels of E. coli/Shigella had a distinctive community structure at the genus level with unique and abundant uncultivated strains of Bacteroidetes. Moreover the same bioreactor was enriched in Aminomonas paucivorans and Clostridia populations that can utilize secondary metabolites produced during cellulose/hemicellulose degradation to generate hydrogen and acetate. Hence specific digestion conditions that enrich for these populations may provide a route to the optimization of co-digestion systems and control the variability in reactor performance.展开更多
Among many ecological services provided by mangrove ecosystems,soil organic carbon(SOC)storages have recently received much attention owing to the increasing atmospheric partial pressure of dissolved CO_(2)(pCO_(2)).B...Among many ecological services provided by mangrove ecosystems,soil organic carbon(SOC)storages have recently received much attention owing to the increasing atmospheric partial pressure of dissolved CO_(2)(pCO_(2)).Bacteria are fundamental to ecosystem functions and strongly influence the coupling of coastal carbon,nitrogen,and sulfur cycling in soils.The SOC storage and bacterial communities along a restored mangrove soil chronosequence in the Jiulong River Estuary were explored using the 16S rDNA sequencing technique.The results showed the SOC storage in the 100 cm soil profile was 103.31±5.87 kg C m^(−2)and 93.10±11.28 kg C m^(−2)for mangroves with afforestation ages of 36 and 60 years,respectively.The total nitrogen(TN)and total sulfur(TS)contents exhibited significant correlations with the SOC in the mangrove soils,but only TN and SOC showed significant correlation in tidal flat soils.Although the tidal flats and mangroves occupied the contiguous intertidal zone within several kilometers,the variations in the SOC storage along the restored mangrove soil chronosequence were notably higher.The Functional Annotation of Prokaryotic Taxa(FAPROTAX)database was used to annotate the metabolic functions of the bacteria in the soils.The annotation revealed that only four metabolic functions were enriched with a higher relative abundance of the corresponding bacteria,and these enriched functions were largely associated with sulfate reduction.In addition,the specifically critical bacterial taxa that were associated with the SOC accumulation and nutrient cycling,shaped the distinct metabolic functions,and consequently facilitated the SOC accumulation in the mangrove soils with various afforestation ages.The general homogenization of the microbial community and composition along the intertidal soil chronosequence was primarily driven by the reciprocating tidal flows and geographical contiguity.展开更多
Communities of bacteria,especially symbionts,are vital for the growth and development of insects and other arthropods,including Bemisia tabaci Mediterranean(MED),a destructive and invasive insect pest.However,the infe...Communities of bacteria,especially symbionts,are vital for the growth and development of insects and other arthropods,including Bemisia tabaci Mediterranean(MED),a destructive and invasive insect pest.However,the infection density patterns and influence factors of bacteria in whiteflies,which mainly include symbionts,remain largely unclear.To reveal the different density patterns of Cardinium in B.tabaci MED popula-tions and the impacts of high temperatures on whiteflies with different Cardinium density infection patterns,2 isofemale lines isolated from B.tabaci MED from the same geo-graphical population of China and from B.tabaci MED collected from other countries and locations were examined using several techniques and methods,including fluorescence in situ hybridization(FISH),quantitative real-time polymerase chain reaction(qPCR),16S rRNA gene sequencing,and 2b-RAD sequencing.The results showed that there were 2 different infection density patterns of Cardinium in B.tabaci MED(including 1 high-density pattern and 1 low-density pattern).For whiteflies with low-density Cardinium,conventional PCR could not detect Cardinium,but the other techniques confirmed that there was a low level of Cardinium within hosts.High temperature significantly decreased the diversity of bacterial communities:the relative titer of Cardinium increased but the density of Rickettsia decreased in the isofemale line with high-density Cardinium.How-ever,high temperature did not influence the diversity and symbiont density in the line with low-density Cardinium.Moreover,high temperature influenced the functions of bacterial communities in whiteflies with high-density Cardinium but did not affect the bacterial functions in whiteflies with low-density Cardinium.Our results provide novel insights into the complex associations between symbionts and host insects.展开更多
To study the effects of combined Cd and Cu pollution on rhizosphere bacterial community.High-throughput sequencing was used to examine the response of rhizosphere bacterial communities to heavy-metal stress under sing...To study the effects of combined Cd and Cu pollution on rhizosphere bacterial community.High-throughput sequencing was used to examine the response of rhizosphere bacterial communities to heavy-metal stress under single and mixed pollution of cadmium(Cd)and copper(Cu).With additions of Cd and Cu,the mean diversity index of rhizosphere bacterial community was in the order Cu alone>Cd-Cu mixtures>Cd alone.In all Cd and Cu treatments,the dominant phyla were Proteobacteria,Actinobacteria,Chloroflexi and Acidobacteria.In the additions with different concentrations of Cd-Cu mixtures,LEfSe indicated that there were differences in the predominant species of rhizosphere bacterial communities.Some genera such as Streptomyces and Microbacterium belonging to Actinobacteria as biomarkers were significantly enriched in both control and treatments,while some genera such as Pseudoxanthomonas and Rhodopseudomonas belonging to Proteobacteria as biomarkers were observed to be enriched in the additions with single and mixture of Cd and Cu.According to the Nonmetric multidimensional scaling(NMDS)analysis,the structure of rhizosphere bacterial community was different between treatments and the CK.Principal Component Analysis(PCA)and permutational multivariate analysis of variance(PERMANOVA)showed that there were significant differences among treatments(p<0.01),and that the addition of Cu might be the primary factor affecting the composition of rhizosphere bacterial communities.展开更多
Background Oils are important sources of energy in pig diets.The combination of oils with different degree of saturation contributes to improve the utilization efficiency of the mixed oils and may reduce the cost of o...Background Oils are important sources of energy in pig diets.The combination of oils with different degree of saturation contributes to improve the utilization efficiency of the mixed oils and may reduce the cost of oil supplemented.An experiment was conducted to evaluate the effects of oils with different degree of saturation on the fat digestibility and corresponding additivity and bacterial community in growing pigs.Methods Eighteen crossbred(Duroc×Landrace×Yorkshire)barrows(initial body weight:29.3±2.8 kg)were surgically fitted with a T-cannula in the distal ileum.The experimental diets included a fat-free basal diet and 5 oil-added diets.The 5 oil-added diets were formulated by adding 6%oil with different ratio of unsaturated to saturated fatty acids(U:S)to the basal diet.The 5 oils were palm oil(U:S=1.2),canola oil(U:S=12.0),and palm oil and canola oil were mixed in different proportions to prepare a combination of U:S of 2.5,3.5 and 4.5,respectively.Results The apparent and standardized ileal digestibility(AID and SID)of fat and fatty acids increased linearly(P<0.05)as the U:S of dietary oils increased except for SID of fat and C18:2.The AID and SID of fat and fatty acids differed among the dietary treatments(P<0.05)except for SID of unsaturated fatty acids(UFA)and C18:2.Fitted one-slope broken-line analyses for the SID of fat,saturated fatty acids(SFA)and UFA indicated that the breakpoint for U:S of oil was 4.14(R^(2)=0.89,P<0.01),2.91(R^(2)=0.98,P<0.01)and 3.84(R^(2)=0.85,P<0.01),respectively.The determined SID of fat,C18:1,C18:2 and UFA in the mixtures was not different from the calculated SID of fat,C18:1,C18:2 and UFA.However,the determined SID of C16:0,C18:0 and SFA in the mixtures were greater than the calculated SID values(P<0.05).The abundance of Romboutsia and Turicibacter in pigs fed diet containing palm oil was greater than that in rapeseed oil treatment group,and the two bacteria were negatively correlated with SID of C16:0,C18:0 and SFA(P<0.05).Conclusions The optimal U:S for improving the utilization efficiency of mixed oil was 4.14.The SID of fat and UFA for palm oil and canola oil were additive in growing pigs,whereas the SID of SFA in the mixture of two oils was greater than the sum of the values of pure oils.Differences in fat digestibility caused by oils differing in degree of saturation has a significant impact on bacterial community in the foregut.展开更多
The micro-sprinkler irrigation mulched(MSM)has been suggested as a novel water-saving approach in con-trolled environment agriculture.However,the effects of microbial community structure and enzyme activity in the rhi...The micro-sprinkler irrigation mulched(MSM)has been suggested as a novel water-saving approach in con-trolled environment agriculture.However,the effects of microbial community structure and enzyme activity in the rhizosphere soil on crop growth under MSM remain unclear.This study conducted a randomized experimen-tal design using greenhouse tomatoes to investigate changes in bacterial community structure and enzyme activity in rhizosphere soil under different irrigation frequencies(F)and amounts(I)of MSM.Thefindings revealed that with the increase of F or I,The total count of soil bacteria in tomatoesfirst rose and then fell in terms of Opera-tional Taxonomic Units(OTUs)classification.Compared to other F,the most abundance of nitrogen and phos-phorus metabolism genes and enzyme activities were observed with a 5-day F.Moreover,the diversity of soil bacterial community structure initially rose before eventually declining with the increase of the I.Applying 1.00 Epan(cumulative evaporation of a 20 cm standard pan)under MSM helped boost the abundance of nitrogen and phosphorus metabolism functional genes in soil bacteria,ensuring higher enzyme activities related to nitro-gen,carbon,and phosphorus metabolism in the rhizosphere soil of tomatoes.Tomatoes’yield initially rose before eventually declining with the increase in F or I,whereas I had a more significant effect on yield.A 1.00%increase in I yielded a minimum of 39.24%increase in tomato yield.The study showed a positive correlation between soil bacterial community,soil enzyme activity,and greenhouse tomato yield under MSM.Considering the results comprehensively,the combined irrigation mode of F of 5 d and I of 1.00 Epan was recommended for greenhouse tomatoes under MSM.This conclusion provides theoretical support for water-saving practices and yield improve-ment in facility agriculture,especially tomato cultivation.展开更多
Microbiota in the gastrointestinal tract (GIT) of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play...Microbiota in the gastrointestinal tract (GIT) of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play an important role in attenuating such changes caused by weaning stress. Therefore, ileal and colonic contents of weaned piglets were used as inocula, mannan oligosaccharide (MOS) or sugar beet pulp (SBP) was supplied as single energy sources to investigate effects of MOS or SBP on the shifts of gastro-intestinal microflora and lactobacilli populations. The universal bacteria- and lactobacilli-specific PCR/denaturing gradient gel electrophoresis (DGGE), cloning and sequencing techniques were used. DGGE profiles of the universal bacteria showed that great changes were found in the position, numbers and intensity of dominant bands after fermentation. The similarity of bacterial community between ileum and colon was increased to 85-97% by MOS or SBP treatment after fermentation from the similarity with 20% before fermentation. MOS treatment significantly increased the bacterial diversity and band number in both ileal and colonic fermentation (P〈0.05). SBP treatment significantly increased the bacterial diversity and band number in colon (P〈0.05). It implies that some species were enriched by the addition of MOS or SBP to increase the similarity and diversity of bacterial community in weaned piglets. Five specific bands appearing in MOS or SBP treatment group after fermentation were cloned and sequenced, the changes of species related to Prevotella and Ruminococcus were observed. Two bands related to uncultured bacterium with 98% similarity were detected by MOS or SBP treatment. However, there were no effects on the similarity, diversity index and lactobacilli species revealed by MOS or SBP treatment. These results imply that MOS or SBP could have beneficial effects on the weaning piglets by stablizing microbiota in the GIT microflora.展开更多
Continuous landscape components along the lateral riverside are affected by both hydrologic connectivity and disconnectivity.In recent years,anthropogenic activities and climate changes have caused wetland shrinkage a...Continuous landscape components along the lateral riverside are affected by both hydrologic connectivity and disconnectivity.In recent years,anthropogenic activities and climate changes have caused wetland shrinkage and land degradation along the lateral riverside of many arid and semiarid regions.Since microorganisms are major drivers of soil biochemical cycling,it is essential to examine soil microbial communities along the lateral landscape continuum to understand their ecosystem functioning and predict future land changes.Here,we collected samples along a lateral riverbed center-riverbed edge-oxbow lake-floodplain-terrace continuum(i.e.,landward distribution)in the Xilin River Basin,Inner Mongolia,China.The floodplain had the highest microbial diversity and heterogeneity,with Bacteroidetes,β-andγ-Proteobacteria being the most abundant taxa.In contrast,the terrace had the lowest microbial diversity and heterogeneity,with Acidobacteria,Actinobacteria,Verrucomicrobia,Gemmatimonadetes,andα-Proteobacteria as the most abundant taxa.Silt particle,salinity,and moisture were the most influential factors for landward variation of bacterial communities along the riverside continuum.Altogether,we demonstrate that dominant bacterial lineages,soil particles,and moisture-related factors are valuable indicators of this continuum,which can be leveraged for the early prediction of drought-induced wetland shrinkage and grassland desertification.展开更多
Pyrene, a representative polycyclic aromatic hydrocarbon (PAH) compound produced mainly from incomplete combustion of fossil fuels, is hazardous to ecosystem health. However, long-term exposure studies did not detec...Pyrene, a representative polycyclic aromatic hydrocarbon (PAH) compound produced mainly from incomplete combustion of fossil fuels, is hazardous to ecosystem health. However, long-term exposure studies did not detect any significant effects of pyrene on soil microorganism. In this study, short-term microcosm experiments were conducted to identify the immediate effect of pyrene on soil bacterial communities. A freshly- collected pristine red soil was spiked with pyrene at 0, 10, 100, 200, and 500 mg.kg-~ and incubated for one day and seven days. The bacterial communities in the incubated soils were analyzed using 16S rRNA sequencing and terminal restriction fragment length polymorphism (T- RFLP) methods. The results revealed high bacterial diversity in both unspiked and pyrene-spiked soils. Only at the highest pyrene-spiking rate of 500 mg.kg-~, two minor bacteria groups of the identified 14 most abundant bacteria groups were completely suppressed. Short-term exposure to pyrene resulted in dominance of Proteobac- teria in soil, followed by Acidobacteria, Firmutes, and Bacteroidetes. Our findings showed that bacterial commu- nity structure did respond to the presence of pyrene but recovered rapidly from the perturbation. The intensity of impact and the rate of recovery showed some pyrene dosage-dependent trends. Our results revealed that differ- ent levels of pyrene may affect the bacterial community structure by suppressing or selecting certain groups of bacteria. It was also found that the bacterial community was most susceptible to pyrene within one day of the chemical addition.展开更多
Boreal forests commonly suff er from nitrogen defi ciency due to low rate of nitrogen mineralization.Biochar may promote soil organic matter decomposition and accelerate nitrogen mineralization.In this study,Illumina ...Boreal forests commonly suff er from nitrogen defi ciency due to low rate of nitrogen mineralization.Biochar may promote soil organic matter decomposition and accelerate nitrogen mineralization.In this study,Illumina NovaSeq sequencing combined with functional annotation of prokaryotic taxa(FAPROTAX)analysis was used to investigate the eff ect of biochar pyrolysis temperatures,the amount of applied biochar,and the period since the biochar application(2-and 3-year)on soil bacterial communities.The results show that biochar pyrolysis temperatures(500℃ and 650℃)and the amount of applied biochar(0.5 kg m^(−2)and 1.0 kg m^(−2))did not change soil properties.Nevertheless,the interaction of biochar pyrolysis temperature and the amount had signifi cant eff ects on bacterial species richness and evenness(P<0.05).The application of biochar produced at 500℃ had a lower abundance of Actinobacteria and Verrucomicrobia,while that produced at 650℃ had a higher abundance of Conexibacter and Phenylobacterium.When biochar produced at 650℃ was applied,applying 0.5 kg m^(−2)had a higher abundance of Cyanobacteria,Conexibacter,and Phenylobacterium than that of 1.0 kg m^(−2)(P<0.05).Functionally,the abundance of the aromatic compound degradation group increased with the extension of application time and increase of pyrolysis temperature.The time since application played an important role in the formation of soil bacterial communities and their functional structure.Long-term studies are necessary to understand the consequence of biochar on bacterial communities in boreal forests.展开更多
基金The National Key Research and Development Program of China under contract No.2020 YFA0607600。
文摘Marine spatial planning(MSP)is designed to divide the sea area into different types of functional zones,to implement corresponding development activities.However,the long-term impacts of anthropogenic activities associated with MSP practice on the marine microbial biosphere are still unclear.Yalu River Estuary,a coastal region in northeast of China,has been divided into fishery&agricultural(F&A)zone,shipping&port(S&P)zone and marine protected area(MPA)zone by a local MSP guideline that has been run for decades.To examine the effects of long-term executed MSP,benthic bacterial communities from different MSP zones were obtained and compared in this study.The results revealed significant differences in the bacterial community structure and predict functions among different zones.Bacterial genera enriched in different zones were identified,including SBR1031 in MPA,Woeseia and Sva0996 in S&P,and Halioglobus in F&A.In addition,correlations between some bacterial genera and sediment pollutants were uncovered.Furthermore,bacteria related to sulphide production were more abundant in the F&A zone,which was according to the accumulation of sulphides in this area.Moreover,bacteria associated with chemoheterotrophy and fermentation were more predominant in the S&P zone,consistent with high levels of organic matter and petroleum caused by shipping.Our findings indicated benthic bacterial communities could bring to light the anthropogenic activity footprints by different activities induced by long-term MSP practice.
基金supported by the National Natural Science Foundation of China(32071751)the National key research and development program(2021YFD220120102)+1 种基金the Natural Science Foundation of Shandong Province(ZR2018ZC08N3)the funds of the Shandong Double Tops Program(Grant No.SYL2017XTTD03).
文摘Sexual dimorphism of plants shapes the diff erent morphology and physiology between males and females.However,it is still unclear whether it infl uences belowground ecological processes.In this study,rhizosphere soil of male and female Populus deltoides and bulk soil were collected from an 18-year plantation(male and female trees mix-planted)and grouped into three soil compartments.Soil carbon(C),nitrogen(N)and phosphorus(P)levels were determined,and soil bacterial communities were analyzed by high-throughput sequencing.The results showed the less total carbon and total organic carbon,the more nutrients(available phosphorus,nitrate nitrogen and ammonium nitrogen)available in the rhizosphere soils of female poplars than soils of males.However,α-diversity indices of the rhizosphere bacterial communities under male plants were signifi-cantly higher.Principal component analysis showed that the bacterial communities were signifi cantly diff erent between the male and female soil compartments.Further,the bacterial co-occurrence network in soil under male trees had more nodes and edges than under females.BugBase analysis showed the more functional bacteria taxa related to biofi lm formation and antioxidation under males.The results indicate that soils under male poplars had more diverse and more complex co-occurrence networks of the rhizosphere bacterial community than soils under female trees,implying that male poplars might have better environmental adaptability.The study provides insight into the diff erent soil-microbe interactions of dioecious plants.More details about the infl uencing mechanism of sexual dimorphism on rhizosphere soil bacterial communities need to be further studied.
基金financially supported by State Environmental Protection Key Laboratory of Microorganism Application and Risk Control(No.MARC2012D010)National Water Special Program(No.2012ZX07404-002)International Science&Technology Cooperation Program of China(No.2010DFA91830)
文摘This study aimed to investigate bacterial community in an urban drinking water distribution system (DWDS) during an occurrence of colored water. Variation in the bacterial community diversity and structure was observed among the different waters, with the predominance of Proteobacteria. While Verrucomicrobia was also a major phylum group in colored water. Limnobacter was the major genus group in colored water, but Undibacterium predominated in normal tap water. The coexistence of Limnobacter as well as Sediminibacterium and Aquobocterium might contribute to the formation of colored water.
基金The National Key R&D Program of China under contract No.2019YFD0901305the Science and Technology Program of Zhoushan under contract No.2019C21011+4 种基金the National Natural Science Foundation of China under contract Nos31270160 and J1310037the Natural Science Foundation of Zhejiang ProvinceChina under contract No.LY12C03003the Zhejiang Public Welfare Technology Application Research Project under contract No.2016C33084the Research Project of Ecological Environment Protection and Restoration of Yangtze River in Zhoushan under contract No.SZGXZS2020068。
文摘The Changjiang River Estuary(CRE)in the East China Sea suffers from seasonal hypoxia in summer.The vertical distributions and seasonal changes of microbial communities in the CRE were well documented.However,little is known about the diurnal changes of bacterial communities in the hypoxic zone of the CRE.Here,16 S rRNA gene analysis was used to explore the changes of bacterial communities in the oxic surface and hypoxic middle seawater layers during 24 h in the CRE.Significant differences between the hypoxic and oxic layers were observed:the phyla Cyanobacteria,Bacteroidetes and Acidimicrobiia were enriched in the oxic layer,whereas the phylum SAR406 and the class Deltaproteobacteria were more abundant in the hypoxic layer.In addition,some subtle diurnal variations of the bacterial relative abundance were found in both two layers.The relative abundance of Synechococcus increased at night,and this change was more obvious in the hypoxic layer.The similar trend was also found in some phototrophic and several heterotrophic bacteria,such as Rhodobacteraceae,OM60 and Flavobacteriaceae.Their relative abundances peaked at 16:00 in the oxic layer,while the relative abundances peaked at around 7:00 and decreased until 13:00 in the hypoxic layer.Together,the results of the present study suggest that some photosynthetic bacteria and several heterotrophic bacteria have similar diurnal variations implying the light and physicochemical heterogeneity in the course of a day are important for bacterial diurnal changes in the CRE.
基金supported by the International Scientific and Technological Cooperation Projects of China (2015DFA20790)the National Natural Science Foundation of China (21577172,41501322)the National Basic Research Program (973 program) of China (2013CB127406)
文摘Coated controlled-release fertilizers (CRFs) have been widely applied in agriculture due to their increased efficiency. However, the widespread and a lot of coated CRFs application may leave undesired coating residues in the soil due to their slow degradation. Limited information is available on the effects of substantial residual coatings on the soil bacterial community. By adding 0, 5, 10, 20, and 50 times quantities of residual coating from conventional application amount of resin and water-soluble coated CRFs, we studied the responses of soil properties and bacterial community composition to these two residual coatings in black soil. The results showed that the resin and water-soluble coatings did not essentially alter the properties of black soil or cause dramatic changes to bacterial diversity within the test concentration range. The residual resin and water-soluble coatings also did not distinctly alter the relative abundance of the top ten bacteria at phylum level. Heatmap results suggested that the treatments were basically clustered into two groups by concentration rather than types of coating material. Pearson correlation analysis showed that the Simpson's diversity index of the bacterial community was significantly correlated with microbial biomass carbon (MBC, r=0.394, P〈0.05), and the richness index abundance-based coverage estimator (ACE) of the bacterial community was significantly correlated with microbial biomass nitrogen (MBN, t=0.407, P〈0.05). Overall, results of this study suggested that substantial residual resin and water-soluble coatings with 0-50 times quantities of residual coating from conventional application amount of coated CRFs did not generate obviously negative impacts on the bacterial community in black soil.
基金The Program for New Century Excellent Talents in University under contract No.NCET-12-1065the National Natural Science Foundation of China under contract Nos 41276124 and 41176136+1 种基金the Science Fund for University Creative Research Groups in Tianjin under contract No.TD12-5003the Program for Changjiang Scholars to J Sun
文摘Besides being critical components of marine food web,microorganisms play vital roles in biogeochemical cycling of nutrients and elements in the ocean.Currently little is known about microbial population structure and their distributions in the eastern Indian Ocean.In this study,we applied molecular approaches including polymerase chain reaction-denaturant gradient gel electrophoresis(PCR-DGGE) and High-Throughput next generation sequencing to investigate bacterial 16S rRNA genes from the equatorial regions and the adjacent Bay of Bengal in the eastern Indian Ocean.In general,Bacteroidetes,Proteobacteria(mainly Alpha,and Gamma),Actinobacteria,Cyanobacteria and Planctomycetes dominated the microbial communities.Horizontally distinct spatial distribution of major microbial groups was observed from PCR-DGGE gel image analyses.However,further detailed characterization of community structures by pyrosequencing suggested a more pronounced stratified distribution pattern:Cyanobacteria and Actinobacteria were more predominant at surface water(25m);Bacteroidetes dominated at 25m and 150m while Proteobacteria(mainly Alphaproteobacteria) occurred more frequently at 75m water depth.With increasing water depth,the bacterial communities from different locations tended to share high similarity,indicating a niche partitioning for minor groups of bacteria recovered with high throughput sequencing approaches.This study provided the first "snapshot" on biodiversity and spatial distribution of Bacteria in water columns in the eastern Indian Ocean,and the findings further emphasized the potential functional roles of these microbes in energy and resource cycling in the eastern Indian Ocean.
基金the National Key Research and Development Program of China(2018YFD0500205)the Strategic Priority Research Program of the Chinese Academy Sciences(XDA23070502)。
文摘Available information on the microbial mechanisms associated with heavy metal(HM)passivation during co-composting amended with phosphate rock(PR)remains limited.Thus,this study investigated the dynamic changes in bacterial communities and HM-fractions(Zn,Cu,Cd,Cr and Pb)during swine manure composting with maize straw,and ascertained the bacterial influence on HM-passivation.The results demonstrated that the addition of PR improved HM-passivation,especially for Zn and Cd,with their bioavailability factors(BFs)reduced by 247.41 and 176.25%,respectively.As for bacterial communities,the proportion of Firmicutes decreased,while the proportions of Proteobacteria,Bacteroidetes,DeinococcusThermus and Gemmatimonadetes increased in all treatments.PR significantly changed the primary bacterial phyla in the thermophilic phase.Bacteroidetes were the main bacterial component controlling the passivation of Zn,Cu and Cr,while Deinococcus-Thermus mainly regulated the mobility of Zn and Pb,and Proteobacteria only dominated the transformation among Cd-fractions.These results may provide a reference for the use of HM-passivation techniques during composting.
基金Supported by the National Key R&D Program of China(No.2017YFD0701700)National Natural Science Foundation of China(Nos.31472312,31672673)。
文摘Bacteria play a major role in metabolizing ammonia and other metabolites in recirculating aquaculture systems(RASs).To characterize and compare the bacterial communities in the biofilters of two full-scale RASs for the culture of puffer fish,Takifugu rubripes,at different ages and densities were studied.In overall,47807 optimized reads of the 16 S rRNA gene with V4-V5 region were obtained from four biofilm samples collected after biofilm maturation.At 97%cut-off level,these sequences were clustered into 500 operational taxonomic units,and were classified into 19 bacterial phyla and 138 genera.At the phylum level,Proteobacteria and Bacteroidetes were the most abundant,followed by Nitrospirae and Planctomycetes.At the genus level,Colwellia,Marinifilum,Oceanospirillum,Lutibacter,Winogradskyella,Pseudoalteromonas,Arcobacter,and Phaeobacter were the top members.Nitrosomonas and Nitrospira were main ammonia-and nitrite-oxidizing bacteria.Differences in bacterial communities at different sampling dates and similarities of both biofilters were revealed in the Venn diagram and cluster analysis.Maintaining a good water quality and health offarmed fish in RASs depended on the correct management of the bacterial communities.This study provides more accurate information on the bacterial communities associated with the bifilters of both RASs.
基金supported by the Major Research Plan of Tianjin (No.16YFXTSF00460)the National Natural Science Foundation of China (No.21878220)
文摘Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.
基金Supported by the National Key Research and Development Program of China(No.2018YFC1406501)the National Natural Science Foundation of China(Nos.U2106208,42003064,41876138)+1 种基金the Key Research and Development Program of Yantai(No.2020MSGY056)the Shandong Key Laboratory of Marine Ecological Restoration(No.201921)。
文摘Bacteria communities in cnidarian jellyfi sh can be harmful to many important aquaculture species,as they can be key vectors of bacterial pathogens.However,our knowledge of bacterial communities associated with jellyfi sh in culture ponds and their potential roles in the regulation of aquaculture species remains unclear.In this study,sequencing based on the bacterial 16S rRNA gene was used to investigate the composition and variation of the bacterial communities associated with hydromedusa Gonionemus vertens in sea cucumber culture ponds and natural marine environment.The associated bacterial communities of G.vertens from the culture ponds in the Yellow Sea and Bohai Sea had signifi cantly diff erent compositions,when compared with those from ambient seawater environment.Furthermore,bacterial communities associated with G.vertens had similar diversity and composition in culture ponds and natural marine environment in the Yellow Sea.There were 31 unique bacterial biomarkers identifi ed in three locations.The major communities were highly abundant in Kiloniellales,Octadecabacter,Polynucleobacter,and Polaribacter,and are related to the environmental information processing.Pathogen candidates such as Vibrionales and Chlamydiales had notably low relative abundances(<1%).The venom of the jellyfi sh was considered responsible for damage to the aquaculture.This study provides important data to help assess the impact of cnidarians-associated bacterial communities on pond aquaculture and the infl uences on material cycling and energy fl ow in marine ecosystems.
文摘Over 3 million tons of manures are produced annually in the United States and pose environmental and health risks if not remediated. Anaerobic digestion is an effective method in treating organic wastes to reduce environmental impacts and produce methane as an alternative energy. Previous studies suggested that optimization of feed composition, hydraulic retention time, and other operational conditions can greatly improve total solids removal and increase methane productivity. These environmental factors improve functionality by altering the microbial community structure but explicit details of how the bacterial community shifts are poorly understood. Our investigations were conducted to investigate the relationship between environmental factors, microbial community structure and bioreactor efficiency by using metagenomic analysis of the microbial communities. Our results indicated that the bioreactor with the greatest methane production, digestion efficiency and reduced levels of E. coli/Shigella had a distinctive community structure at the genus level with unique and abundant uncultivated strains of Bacteroidetes. Moreover the same bioreactor was enriched in Aminomonas paucivorans and Clostridia populations that can utilize secondary metabolites produced during cellulose/hemicellulose degradation to generate hydrogen and acetate. Hence specific digestion conditions that enrich for these populations may provide a route to the optimization of co-digestion systems and control the variability in reactor performance.
基金the National Natural Science Foundation of China(42230407)the National Key R&D Program of China(2016YFC0502900)the Scientific Research Project of Anhui Institute of Environmental Sciences(HKYKY2020-02).
文摘Among many ecological services provided by mangrove ecosystems,soil organic carbon(SOC)storages have recently received much attention owing to the increasing atmospheric partial pressure of dissolved CO_(2)(pCO_(2)).Bacteria are fundamental to ecosystem functions and strongly influence the coupling of coastal carbon,nitrogen,and sulfur cycling in soils.The SOC storage and bacterial communities along a restored mangrove soil chronosequence in the Jiulong River Estuary were explored using the 16S rDNA sequencing technique.The results showed the SOC storage in the 100 cm soil profile was 103.31±5.87 kg C m^(−2)and 93.10±11.28 kg C m^(−2)for mangroves with afforestation ages of 36 and 60 years,respectively.The total nitrogen(TN)and total sulfur(TS)contents exhibited significant correlations with the SOC in the mangrove soils,but only TN and SOC showed significant correlation in tidal flat soils.Although the tidal flats and mangroves occupied the contiguous intertidal zone within several kilometers,the variations in the SOC storage along the restored mangrove soil chronosequence were notably higher.The Functional Annotation of Prokaryotic Taxa(FAPROTAX)database was used to annotate the metabolic functions of the bacteria in the soils.The annotation revealed that only four metabolic functions were enriched with a higher relative abundance of the corresponding bacteria,and these enriched functions were largely associated with sulfate reduction.In addition,the specifically critical bacterial taxa that were associated with the SOC accumulation and nutrient cycling,shaped the distinct metabolic functions,and consequently facilitated the SOC accumulation in the mangrove soils with various afforestation ages.The general homogenization of the microbial community and composition along the intertidal soil chronosequence was primarily driven by the reciprocating tidal flows and geographical contiguity.
基金supported by the National Natural Science Foundation of China(31872030)the Taishan Scholar Foundation of Shandong Province of China,the First Class Grassland Science Discipline Program of Shandong Province,China,and the Qingdao Agricultural University High-level Talent Fund(663-1121025).
文摘Communities of bacteria,especially symbionts,are vital for the growth and development of insects and other arthropods,including Bemisia tabaci Mediterranean(MED),a destructive and invasive insect pest.However,the infection density patterns and influence factors of bacteria in whiteflies,which mainly include symbionts,remain largely unclear.To reveal the different density patterns of Cardinium in B.tabaci MED popula-tions and the impacts of high temperatures on whiteflies with different Cardinium density infection patterns,2 isofemale lines isolated from B.tabaci MED from the same geo-graphical population of China and from B.tabaci MED collected from other countries and locations were examined using several techniques and methods,including fluorescence in situ hybridization(FISH),quantitative real-time polymerase chain reaction(qPCR),16S rRNA gene sequencing,and 2b-RAD sequencing.The results showed that there were 2 different infection density patterns of Cardinium in B.tabaci MED(including 1 high-density pattern and 1 low-density pattern).For whiteflies with low-density Cardinium,conventional PCR could not detect Cardinium,but the other techniques confirmed that there was a low level of Cardinium within hosts.High temperature significantly decreased the diversity of bacterial communities:the relative titer of Cardinium increased but the density of Rickettsia decreased in the isofemale line with high-density Cardinium.How-ever,high temperature did not influence the diversity and symbiont density in the line with low-density Cardinium.Moreover,high temperature influenced the functions of bacterial communities in whiteflies with high-density Cardinium but did not affect the bacterial functions in whiteflies with low-density Cardinium.Our results provide novel insights into the complex associations between symbionts and host insects.
文摘To study the effects of combined Cd and Cu pollution on rhizosphere bacterial community.High-throughput sequencing was used to examine the response of rhizosphere bacterial communities to heavy-metal stress under single and mixed pollution of cadmium(Cd)and copper(Cu).With additions of Cd and Cu,the mean diversity index of rhizosphere bacterial community was in the order Cu alone>Cd-Cu mixtures>Cd alone.In all Cd and Cu treatments,the dominant phyla were Proteobacteria,Actinobacteria,Chloroflexi and Acidobacteria.In the additions with different concentrations of Cd-Cu mixtures,LEfSe indicated that there were differences in the predominant species of rhizosphere bacterial communities.Some genera such as Streptomyces and Microbacterium belonging to Actinobacteria as biomarkers were significantly enriched in both control and treatments,while some genera such as Pseudoxanthomonas and Rhodopseudomonas belonging to Proteobacteria as biomarkers were observed to be enriched in the additions with single and mixture of Cd and Cu.According to the Nonmetric multidimensional scaling(NMDS)analysis,the structure of rhizosphere bacterial community was different between treatments and the CK.Principal Component Analysis(PCA)and permutational multivariate analysis of variance(PERMANOVA)showed that there were significant differences among treatments(p<0.01),and that the addition of Cu might be the primary factor affecting the composition of rhizosphere bacterial communities.
基金supported by National Natural Science Foundation of China(31972597 and 32302793)。
文摘Background Oils are important sources of energy in pig diets.The combination of oils with different degree of saturation contributes to improve the utilization efficiency of the mixed oils and may reduce the cost of oil supplemented.An experiment was conducted to evaluate the effects of oils with different degree of saturation on the fat digestibility and corresponding additivity and bacterial community in growing pigs.Methods Eighteen crossbred(Duroc×Landrace×Yorkshire)barrows(initial body weight:29.3±2.8 kg)were surgically fitted with a T-cannula in the distal ileum.The experimental diets included a fat-free basal diet and 5 oil-added diets.The 5 oil-added diets were formulated by adding 6%oil with different ratio of unsaturated to saturated fatty acids(U:S)to the basal diet.The 5 oils were palm oil(U:S=1.2),canola oil(U:S=12.0),and palm oil and canola oil were mixed in different proportions to prepare a combination of U:S of 2.5,3.5 and 4.5,respectively.Results The apparent and standardized ileal digestibility(AID and SID)of fat and fatty acids increased linearly(P<0.05)as the U:S of dietary oils increased except for SID of fat and C18:2.The AID and SID of fat and fatty acids differed among the dietary treatments(P<0.05)except for SID of unsaturated fatty acids(UFA)and C18:2.Fitted one-slope broken-line analyses for the SID of fat,saturated fatty acids(SFA)and UFA indicated that the breakpoint for U:S of oil was 4.14(R^(2)=0.89,P<0.01),2.91(R^(2)=0.98,P<0.01)and 3.84(R^(2)=0.85,P<0.01),respectively.The determined SID of fat,C18:1,C18:2 and UFA in the mixtures was not different from the calculated SID of fat,C18:1,C18:2 and UFA.However,the determined SID of C16:0,C18:0 and SFA in the mixtures were greater than the calculated SID values(P<0.05).The abundance of Romboutsia and Turicibacter in pigs fed diet containing palm oil was greater than that in rapeseed oil treatment group,and the two bacteria were negatively correlated with SID of C16:0,C18:0 and SFA(P<0.05).Conclusions The optimal U:S for improving the utilization efficiency of mixed oil was 4.14.The SID of fat and UFA for palm oil and canola oil were additive in growing pigs,whereas the SID of SFA in the mixture of two oils was greater than the sum of the values of pure oils.Differences in fat digestibility caused by oils differing in degree of saturation has a significant impact on bacterial community in the foregut.
基金funded by the Natural Science Foundation of China(No.41807041)the Science and Technology Research Project of Henan Province(242102111101)the Mechanical Design,Manufacturing,and Automation Key Discipline of Henan Province(JG[2018]No.119).
文摘The micro-sprinkler irrigation mulched(MSM)has been suggested as a novel water-saving approach in con-trolled environment agriculture.However,the effects of microbial community structure and enzyme activity in the rhizosphere soil on crop growth under MSM remain unclear.This study conducted a randomized experimen-tal design using greenhouse tomatoes to investigate changes in bacterial community structure and enzyme activity in rhizosphere soil under different irrigation frequencies(F)and amounts(I)of MSM.Thefindings revealed that with the increase of F or I,The total count of soil bacteria in tomatoesfirst rose and then fell in terms of Opera-tional Taxonomic Units(OTUs)classification.Compared to other F,the most abundance of nitrogen and phos-phorus metabolism genes and enzyme activities were observed with a 5-day F.Moreover,the diversity of soil bacterial community structure initially rose before eventually declining with the increase of the I.Applying 1.00 Epan(cumulative evaporation of a 20 cm standard pan)under MSM helped boost the abundance of nitrogen and phosphorus metabolism functional genes in soil bacteria,ensuring higher enzyme activities related to nitro-gen,carbon,and phosphorus metabolism in the rhizosphere soil of tomatoes.Tomatoes’yield initially rose before eventually declining with the increase in F or I,whereas I had a more significant effect on yield.A 1.00%increase in I yielded a minimum of 39.24%increase in tomato yield.The study showed a positive correlation between soil bacterial community,soil enzyme activity,and greenhouse tomato yield under MSM.Considering the results comprehensively,the combined irrigation mode of F of 5 d and I of 1.00 Epan was recommended for greenhouse tomatoes under MSM.This conclusion provides theoretical support for water-saving practices and yield improve-ment in facility agriculture,especially tomato cultivation.
基金funded by the National Basic Research Program of China (973 Program,2004CB117500)
文摘Microbiota in the gastrointestinal tract (GIT) of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play an important role in attenuating such changes caused by weaning stress. Therefore, ileal and colonic contents of weaned piglets were used as inocula, mannan oligosaccharide (MOS) or sugar beet pulp (SBP) was supplied as single energy sources to investigate effects of MOS or SBP on the shifts of gastro-intestinal microflora and lactobacilli populations. The universal bacteria- and lactobacilli-specific PCR/denaturing gradient gel electrophoresis (DGGE), cloning and sequencing techniques were used. DGGE profiles of the universal bacteria showed that great changes were found in the position, numbers and intensity of dominant bands after fermentation. The similarity of bacterial community between ileum and colon was increased to 85-97% by MOS or SBP treatment after fermentation from the similarity with 20% before fermentation. MOS treatment significantly increased the bacterial diversity and band number in both ileal and colonic fermentation (P〈0.05). SBP treatment significantly increased the bacterial diversity and band number in colon (P〈0.05). It implies that some species were enriched by the addition of MOS or SBP to increase the similarity and diversity of bacterial community in weaned piglets. Five specific bands appearing in MOS or SBP treatment group after fermentation were cloned and sequenced, the changes of species related to Prevotella and Ruminococcus were observed. Two bands related to uncultured bacterium with 98% similarity were detected by MOS or SBP treatment. However, there were no effects on the similarity, diversity index and lactobacilli species revealed by MOS or SBP treatment. These results imply that MOS or SBP could have beneficial effects on the weaning piglets by stablizing microbiota in the GIT microflora.
基金the National Natural Science Foundation of China to J.Yu(41361053),X.Tanggood(31660724)and Y.Yang(41825016)the Natural Science Foundation of Inner Mongolia to J.Yu(2011MS0603,2016MS0331)and X.Tanggood(2015MS0306)the National College Students Innovation and Entrepreneurship Training Program of Inner Mongolia University to C.H.Li,X.Li,W.Z.Wu,W.Dong and Y.Jia(201810126043).
文摘Continuous landscape components along the lateral riverside are affected by both hydrologic connectivity and disconnectivity.In recent years,anthropogenic activities and climate changes have caused wetland shrinkage and land degradation along the lateral riverside of many arid and semiarid regions.Since microorganisms are major drivers of soil biochemical cycling,it is essential to examine soil microbial communities along the lateral landscape continuum to understand their ecosystem functioning and predict future land changes.Here,we collected samples along a lateral riverbed center-riverbed edge-oxbow lake-floodplain-terrace continuum(i.e.,landward distribution)in the Xilin River Basin,Inner Mongolia,China.The floodplain had the highest microbial diversity and heterogeneity,with Bacteroidetes,β-andγ-Proteobacteria being the most abundant taxa.In contrast,the terrace had the lowest microbial diversity and heterogeneity,with Acidobacteria,Actinobacteria,Verrucomicrobia,Gemmatimonadetes,andα-Proteobacteria as the most abundant taxa.Silt particle,salinity,and moisture were the most influential factors for landward variation of bacterial communities along the riverside continuum.Altogether,we demonstrate that dominant bacterial lineages,soil particles,and moisture-related factors are valuable indicators of this continuum,which can be leveraged for the early prediction of drought-induced wetland shrinkage and grassland desertification.
文摘Pyrene, a representative polycyclic aromatic hydrocarbon (PAH) compound produced mainly from incomplete combustion of fossil fuels, is hazardous to ecosystem health. However, long-term exposure studies did not detect any significant effects of pyrene on soil microorganism. In this study, short-term microcosm experiments were conducted to identify the immediate effect of pyrene on soil bacterial communities. A freshly- collected pristine red soil was spiked with pyrene at 0, 10, 100, 200, and 500 mg.kg-~ and incubated for one day and seven days. The bacterial communities in the incubated soils were analyzed using 16S rRNA sequencing and terminal restriction fragment length polymorphism (T- RFLP) methods. The results revealed high bacterial diversity in both unspiked and pyrene-spiked soils. Only at the highest pyrene-spiking rate of 500 mg.kg-~, two minor bacteria groups of the identified 14 most abundant bacteria groups were completely suppressed. Short-term exposure to pyrene resulted in dominance of Proteobac- teria in soil, followed by Acidobacteria, Firmutes, and Bacteroidetes. Our findings showed that bacterial commu- nity structure did respond to the presence of pyrene but recovered rapidly from the perturbation. The intensity of impact and the rate of recovery showed some pyrene dosage-dependent trends. Our results revealed that differ- ent levels of pyrene may affect the bacterial community structure by suppressing or selecting certain groups of bacteria. It was also found that the bacterial community was most susceptible to pyrene within one day of the chemical addition.
基金funded by The Foundation for Research of Natural Resources in Finland(2016085)supported by the Academy of Finland(286685,294600,307222,277623)the FCoE of atmospheric sciences(Center of Excellence(1118615)).
文摘Boreal forests commonly suff er from nitrogen defi ciency due to low rate of nitrogen mineralization.Biochar may promote soil organic matter decomposition and accelerate nitrogen mineralization.In this study,Illumina NovaSeq sequencing combined with functional annotation of prokaryotic taxa(FAPROTAX)analysis was used to investigate the eff ect of biochar pyrolysis temperatures,the amount of applied biochar,and the period since the biochar application(2-and 3-year)on soil bacterial communities.The results show that biochar pyrolysis temperatures(500℃ and 650℃)and the amount of applied biochar(0.5 kg m^(−2)and 1.0 kg m^(−2))did not change soil properties.Nevertheless,the interaction of biochar pyrolysis temperature and the amount had signifi cant eff ects on bacterial species richness and evenness(P<0.05).The application of biochar produced at 500℃ had a lower abundance of Actinobacteria and Verrucomicrobia,while that produced at 650℃ had a higher abundance of Conexibacter and Phenylobacterium.When biochar produced at 650℃ was applied,applying 0.5 kg m^(−2)had a higher abundance of Cyanobacteria,Conexibacter,and Phenylobacterium than that of 1.0 kg m^(−2)(P<0.05).Functionally,the abundance of the aromatic compound degradation group increased with the extension of application time and increase of pyrolysis temperature.The time since application played an important role in the formation of soil bacterial communities and their functional structure.Long-term studies are necessary to understand the consequence of biochar on bacterial communities in boreal forests.