Responses of soil microbial activities to elevated CO, in experiment sites of Pinus sylvestriformis and Pinus koratensts seecllmgs were studied in summer in 2003. The results indicated the number of bacteria decreased...Responses of soil microbial activities to elevated CO, in experiment sites of Pinus sylvestriformis and Pinus koratensts seecllmgs were studied in summer in 2003. The results indicated the number of bacteria decreased significantly （p 〈 0.05） under elevated CO, for Pinus syivestriformis and Pinups koraiensis. Amylase and invertase activities in soil increased for Pinus syivestriformis and decreased for Pinus koraiensis with CO2 enrichment compared with those at ambient （350 pmol·mol^-1）. The size of microbial biomass C also decreased significantly at 700 μmol- mol^-1 CO2. Bacterial community structure had some evident changes under elevated CO, by DGGE （Denaturing Gradient Gel Electrophoresis） analysis of bacterial 16S rDNA gene fragments amplified by PCR from DNA extracted directly from soil. The results suggested that responses of soil microorganisms to elevated CO2 would be related to plant species exposed to elevated CO2.展开更多
基金The study was supported by Major State Basic Research Development Program of China (2002CB412502) and the Knowledge Inno-vation Project from Chinese Academy of Sciences (KZCX1-SW-01-03).
文摘Responses of soil microbial activities to elevated CO, in experiment sites of Pinus sylvestriformis and Pinus koratensts seecllmgs were studied in summer in 2003. The results indicated the number of bacteria decreased significantly （p 〈 0.05） under elevated CO, for Pinus syivestriformis and Pinups koraiensis. Amylase and invertase activities in soil increased for Pinus syivestriformis and decreased for Pinus koraiensis with CO2 enrichment compared with those at ambient （350 pmol·mol^-1）. The size of microbial biomass C also decreased significantly at 700 μmol- mol^-1 CO2. Bacterial community structure had some evident changes under elevated CO, by DGGE （Denaturing Gradient Gel Electrophoresis） analysis of bacterial 16S rDNA gene fragments amplified by PCR from DNA extracted directly from soil. The results suggested that responses of soil microorganisms to elevated CO2 would be related to plant species exposed to elevated CO2.
