objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. co...objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. coli 026:B6 LPS was injected at a dosage of 12. 5 mg/kg through the artery to reproduce endo toxic shock. BPIP at a dosage of 5 mg/kg (BPIP-treated group) or equal volume of normal saline (control group) were injected immediately after the injection of LPS. Results: ①Survival time of the shocked animals was prolonged and the 24 h survival rate was also significantly increased in BPIP-treated group as compared with the control group. ②The mean arterial pressure, left intraventricular systolic pressure, isovolemic ven tricular pressure and ±dp/dtmax. were significantly higher in BPIP-treated group than in control group. ③ Plasma levels of glutamic-pyruvic transaminase and urea nitrogen were markedly higher but those of endotox in and TNFα were lower in BPIP-treated group than in control group. Conclusion: BPIP can exert significant protective effects on cardiac, hepatic and renal functions in rats after endotoxic shock, indicating that BPIP might be a good choice in treatment of sepsis/septic shock.展开更多
Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that...Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that BPI administration could improve hepatic and renal functions after hemorrhagic shock, andenhance the survival rate of the rats with shock (BPI group: 81 % vs physiological saline group: 44 %, P <0.05). In BPI group, the plasma endotoxin level was not significantly changed (0. 20± 0. 04 at the end of resuscitation vs 0. 24± 0. 05 U/ml before shock). Tumor necrosis factor. and interleukin-6 levels in BPI group, although higher after shock and resuscitation, were significantly lower than those in physiologia saline group.It is suggested that BPI exerts a protective effects on rats with hernorrhagic shock, which might be due to itsaction against hemorrhage-induced endotoxin translocation and its inhibition of cytokine responses in shock,展开更多
The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and...The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and selection by methotrexate,the cell lines expressing the fusion protein were obtained.The recombinant protein was purified using cation\|exchange chromatography and its bioactivity was proved with bactericidal assays.展开更多
文摘objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. coli 026:B6 LPS was injected at a dosage of 12. 5 mg/kg through the artery to reproduce endo toxic shock. BPIP at a dosage of 5 mg/kg (BPIP-treated group) or equal volume of normal saline (control group) were injected immediately after the injection of LPS. Results: ①Survival time of the shocked animals was prolonged and the 24 h survival rate was also significantly increased in BPIP-treated group as compared with the control group. ②The mean arterial pressure, left intraventricular systolic pressure, isovolemic ven tricular pressure and ±dp/dtmax. were significantly higher in BPIP-treated group than in control group. ③ Plasma levels of glutamic-pyruvic transaminase and urea nitrogen were markedly higher but those of endotox in and TNFα were lower in BPIP-treated group than in control group. Conclusion: BPIP can exert significant protective effects on cardiac, hepatic and renal functions in rats after endotoxic shock, indicating that BPIP might be a good choice in treatment of sepsis/septic shock.
文摘Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that BPI administration could improve hepatic and renal functions after hemorrhagic shock, andenhance the survival rate of the rats with shock (BPI group: 81 % vs physiological saline group: 44 %, P <0.05). In BPI group, the plasma endotoxin level was not significantly changed (0. 20± 0. 04 at the end of resuscitation vs 0. 24± 0. 05 U/ml before shock). Tumor necrosis factor. and interleukin-6 levels in BPI group, although higher after shock and resuscitation, were significantly lower than those in physiologia saline group.It is suggested that BPI exerts a protective effects on rats with hernorrhagic shock, which might be due to itsaction against hemorrhage-induced endotoxin translocation and its inhibition of cytokine responses in shock,
文摘The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and selection by methotrexate,the cell lines expressing the fusion protein were obtained.The recombinant protein was purified using cation\|exchange chromatography and its bioactivity was proved with bactericidal assays.
