The Acute Toxicity Test in Mice and Bacteriostasis in vitro of Yandureqing and Its Microemulsion

[Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing(AEE)and its microemulsion(AEE-ME).[Method]The acute toxicity was tested in mice by intragastric administration,and median lethal dose(LD50)as well as its 95% confidence interval was calculated by modified Karber method;the bacteriostasis was investigated by cylinder plate method.[Result]LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg;and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4.388-6.566 g/kg.The MICs of AEE to Escherichia coli O149 from swine,Staphylococcus aureus,Salmonella pullorum and Streptococcus agalactiae were 10.00,20.00,20.00 and 10.00 mg/ml,respectively;while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00,10.00,5.00 and 5.00 mg/ml in turn,and that to Pseudomonas aeruginosa is 20.00 mg/ml.[Conclusion]AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation.AEE and AEE-ME have obvious bacteriostasis,in which AEE-ME is superior to AEE.

Study on In vitro Bacteriostasis of the Different Extract from Ilex Kudingcha C.J.Tseng

[ Objective ] The aim was to study bacteriostatic activity of the different extract from Ilex Kudingcha C. J. Tseng. [ Method ] By using test tube 2 -fold dilution method and Kirby-Baueer Disc Diffusionto,we conducted inhibitory test on S. aureus and E, coil and determined the minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC）and the diameter of inhibition zone. [ Result ] The extract 1 had fairly strong in vitro bacteriostasis activities and than the extract2 and extract3. The MIC and MBC was 3.91 mg/ml and 31.25 mg/ml on S. aureus and E. coil [ Conclusion] The result showed that the different extract from Ilex Kudingcha C. J. Tseng had varying amount of different bacteriostatic activities,the Ilex Kudingcha C. J. Tseng was developed new and safe bacteriostatic reagent to provide reference.

Research Progress on Bacteriostasis of Nano Selenium

Nano selenium which has the advantages of low toxicity,strong activity and high biocompatibility has broad-spectrum antibacterial activity to bacteria,fungi,viruses and parasites,and has a broad application prospect in the field of inhibiting microbial infection.This paper mainly reviews the progress in the types and mechanisms of selenium inhibition microorganisms,and prospects the development of the antibacterial activities of selenium nanoparticles.

Associated Bacteriostasis Research of Coptidis Rhizoma-Folium Isatidis and Coptidis Rhizoma-Flos Poprli against Escherichia coli O2

[Objective] The paper was to explore the combined inhibitory effect of rhizoma coptidis-folium isatidis and rhizoma coptidis-flos poprli against Escherichia coli O2.[Method] Contrast test of single and associated bacteriostasis against known serotype E. coli O2 was conducted using microcheckerboard method.[Result] The MIC of rhizoma coptidis, folium isatidis and flos poprli were 1/8 extracting liquid, 1/8 extracting liquid and 1/2 extracting liquid, respectively. When combined with folium isatidis or flos poprli, the MIC of rhizoma coptidis was 1/8 extracting liq-uid or 1/16 extracting liquid compared with single use. When combined with rhizoma coptidis, the MIC of folium isatidis and flos poprli were 1/8 extracting liquid and 1/16 extracting liquid.[Conclusion] When rhizoma coptidis was combined with folium isatidis or flos poprli, the FIC values were 2 and 0.625, performing independent action and additive effect, respectively.

六味能消丸TLC鉴别及3种蒽醌类成分的含量测定

目的建立六味能消丸的TLC鉴别方法,并采用一测多评法同时测定其中大黄素、大黄酚、大黄素甲醚的含量。方法采用TLC鉴别干姜、大黄药材。分析采用Phenomenex C_(18)色谱柱(150 mm×4.6 mm,5μm);流动相甲醇-0.2%H_(3)PO_(4)溶液(85∶15);体积流量1.0 mL/min;柱温30℃;检测波长254 nm。以大黄酚为内标,计算其他2种成分的相对校正因子,测定其含量。结果TLC斑点清晰,分离度良好。3种成分在各自范围内线性关系良好(r≥0.9998),平均加样回收率98.89%~101.11%,RSD 1.31%~1.69%。一测多评法所得结果与外标法接近。结论该方法准确稳定,重复性好,可用于六味能消丸的质量控制。

TLC-SERS同时检测降压类保健食品中五种沙坦类违禁添加成分

由于人们不健康的饮食结构和生活作息习惯以及人口老龄化等因素,高血压等慢性病发病率逐渐增高,降压类保健食品逐渐受到人们青睐。但是市场中存在不良商家为了提高保健食品的功效,向保健食品中非法添加降压药,尤其是沙坦类降压药的现象。建立了一种薄层色谱层析技术(TLC)与表面增强拉曼光谱(SERS)联用方法,对降压类保健食品中五种可能添加的沙坦类违禁成分(奥美沙坦酯、厄贝沙坦、氯沙坦钾、替米沙坦、缬沙坦)进行同步检测。对五种药物的SERS检测最佳条件进行探究,并通过比较不同成分及配比的TLC展开剂对于五种药物的分离效果,经过大量配方试验后成功得到环己烷-乙酸乙酯-甲醇-冰醋酸=53∶35∶12∶0.25(v/v/v/v)的分离体系,对五种药物具有良好的分离效果。确定实验条件后利用TLC分离技术对样品溶液分别进行初步分离,在薄层板上不同比移值的斑点原位处滴加金胶进行SERS检测,对样品溶液在斑点原位处的SERS光谱进行采集、比较,确定。研究结果表明,五种沙坦类违禁添加成分可通过TLC明确分离,且其SERS光谱特征峰一致、重复性良好,根据各自的特征峰可快速定性分析,检出限在0.1~1 mg·kg^(-1)之间。研究建立的TLC-SERS方法简单、快速,能够为保健食品中违禁添加成分的现场初步快速筛查提供一定参考。

TLC Identification of Yao Medicine Pileostegia tomentellal and Extraction Technology and Content Determination of Umbelliferone

[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal.

Establishment of a TLC Identification Method for Ensete wilsonii

[Objectives]The paper was to establish a TLC identification method for Ensete wilsonii.[Methods]Usingβ-sitosterol as the reference,the effects of preparation methods of test solutions,developing solvents,developing distances and color developing agents on TLC analysis were investigated,and the best TLC conditions for E.wilsonii were determined.[Results]The test solution prepared with 90%methanol solvent was dotted on TLC silica gel G plate,and developed with dichloromethane-toluene-methanol=10:5:1.5 as the developing solvent.Then the plate was sprayed with 10%sulfuric acid ethanol solution,and dried with hot blast for color development.Finally,the plate was examined under an ultraviolet lamp at 365 nm.The TLC results of E.wilsonii obtained showed good separation and color development effect,and the spots were clear and characteristic.[Conclusions]This method is safe,specific,and easy to operate,and can be used as a TLC identification method for E.wilsonii.

TLC Identification and Extraction Process of Rubiasin-1-methyl Ether from Yao Medicine Chuanlianzhu

[Objectives]To establish a thin-layer chromatography(TLC)method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu(Damnacanthus giganteus).[Methods]A silica gel G thin-layer plate was adopted for TLC.Petroleum ether(60-90℃)-chloroform-methanol-water(7:15:3:1)was used as the developing solvent and inspected under ultraviolet lamp(365 nm).The content was determined by Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm),mobile phase:acetonitrile-0.2%phosphoric acid gradient elution,detection wavelength 277 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control,and the spots were clear and specific.The injection volume of rubiadin-1-methyl ether showed a good linear relationship in the range of 2.90-145μg(R=0.9996).The average recovery rate of rubiadin-1-methyl ether in the low,medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%,and RSD=1.78%.[Conclusions]This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1-methyl ether in the medicinal materials.It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources.

牙膏中三七皂苷TLC定性检测法的改进

目的:寻找、建立一个磷酸氢钙与二氧化硅复配体系牙膏中三七皂苷检测薄层色谱的方法与规程,适用于检测、验证牙膏成品中含有三七根酊、三七花酊、三七总皂苷、三七叶皂苷。方法:以过滤、化学反应、萃取、吸附、洗脱等原理分离出牙膏中存在的三七根、叶、花皂苷,并制成供试样品,通过比较供试样品与对照样品在硅胶板上显现的点位,进行结果判断,确认该成品是否符合配方和生产工艺加工制出。结果:供试品与对照品以三氯甲烷-乙酸乙酯-甲醇-水(15∶40∶22∶10)为展开剂,5℃~10℃展开,喷以10%硫酸乙醇溶液显色,供试品中主要成分的色谱点,与对照品色谱点应显相同位置;置紫外光灯(365nm)下检视,显相同的荧光斑点。结论:通过本次方法学改进,确认符合磷酸氢钙与二氧化硅复配体系牙膏的TLC检测方法。

TLC插卡型模板早拆体系在施工中的应用

本文通过对TLC插卡型模板早拆体系在施工中的应用展开探讨,旨在分析技术应用效果以及优化应用策略,确保其技术效用的充分发挥。通过对相关文献的综合调研和分析,在施工现场使用TLC插卡型模板早拆体系,并结合实际案例探讨其应用流程。经过实践证明,TLC插卡型模板早拆体系能够显著提高施工效率。由于其设计先进,可灵活拆卸的特点,大大缩短了施工周期,节约了人力资源和材料费用。此外,该体系还具有较高的稳定性,能够有效防止模板的倾倒和坍塌等意外事故。综上所述,TLC插卡型模板早拆体系在施工中具有广阔的应用前景。为了充分发挥TLC插卡型模板早拆体系的优势,需要确保各环节施工作业的高效开展,这样一来才能够保障工程项目的整体建设效果。由此可见,在今后的建筑施工中,值得进一步推广和应用这种模板体系,以满足不断增长的建筑需求,推动建筑行业的健康可持续发展。

TLC插卡型模板早拆体系施工方法

文章立足于TLC插卡型模板特点,围绕该模板早拆体系的施工方法展开探讨,旨在进一步提高该模板体系的应用效率,为施工作业的高效开展奠定良好的基础。本文结合大量的文献资料,严格按照设计要求开展模板搭建、混凝土浇筑以及模板拆除等施工作业。TLC插卡型模板早拆体系的应用,应严格遵守相关标准要求,并对其进行科学优化和调整。研究结果表明,TLC插卡型模板早拆施工体系的应用,在缩短施工工期、提升施工效率等方面都发挥着非常关键的作用。而且该方法还具有可重复使用的特点,大大提升了建筑工程项目的节能环保效果,为后期建筑行业的健康可持续发展提供了可靠的保障。总之,TLC插卡型早拆体系使用方法值得在建筑工程项目建设中得到全面推广应用。希望通过本文的探究,能够为相关工作的开展起到参考作用。

几种单糖、寡糖TLC条件的确立及草石蚕寡糖中水苏糖含量测定

目的:建立薄层色谱(TLC)分析几种单糖、寡糖的有效方法,并测定草石蚕寡糖中水苏糖含量。方法:以果糖、蔗糖、棉子糖和水苏糖为研究对象,从斑点分离度、斑点集中与否、有无拖尾现象及展开时间几方面探讨其在不同体系下的薄层色谱行为;通过薄层色谱扫描(TLCS)测定草石蚕寡糖中水苏糖含量。结果:确立适合的展开系统为乙酸乙酯/甲醇/水/冰乙酸(11.5:3.2:2:3.2),合适的显色剂为茴香醛-浓硫酸显色剂;草石蚕寡糖中水苏糖含量为69.3%。

知母中知母皂苷BⅡ的TLC鉴别及HPLC法含量测定

目的:建立知母药材中知母皂苷BⅡ的 TLC 鉴别及 HPLC 含量测定方法。方法:TLC 法,展开剂:氯仿-甲醇-水(65:35:10)10℃以下放置的下层溶液为展开剂,显色剂:10%硫酸乙醇溶液,105℃加热至斑点显色清晰。HPLC 法,色谱柱:HiQ-sil C_(18)(5μm,4.6mm×250mm),流动相:甲醇-水(53:47),流速:0.8 mL·min^(-1),检测:蒸发光散射检测器(ELSD),漂移管温度:100℃,气体流速:2.6L·min^(-1)。结果:3批药材中知母皂苷 BⅡ成分的 TLC 分离效果均较好,HPLC 法测得知母皂苷 BⅡ的平均回收率为97.7%,RSD 为1.8%。结论:该方法准确可靠,可用于知母药材质量控制。

冬虫夏草及人工虫草核苷类成分的TLCS研究

目的 :以腺苷、尿苷、鸟苷为指标测定天然虫草与人工虫草菌丝体中核苷类有效成分的含量 ,并对不同虫草样品进行薄层色谱指纹图谱对比研究 ,探求虫草中核苷类化合物鉴别的专属性。方法 :采用高效薄层色谱扫描法进行含量测定 ,同时建立了不同虫草样品的荧光淬灭薄层色谱指纹图谱。结果 :虫草菌丝体中测得腺苷、尿苷鸟苷成分高于天然虫草。结论 :该法快速简便 ,重现性好 ,能有效鉴别出虫草中核苷类成分以及评价不同虫草样品的质量。

应用TLC-FT-SERS对萝芙木中利血平成分的研究

本文报道了应用薄层色谱（ＴＬＣ） 与傅里叶变换表面增强拉曼光谱（ＦＴ－ＳＥＲＳ） 联用技术， 获得了中草药萝芙木有效成分利血平光谱研究的新方法。研究表明：在薄层原位约２ μｇ 的样品即可获得利血平分子的特征振动谱带，并阐述了样品分子在表面增强基底银微粒表面的吸附模式。

TLCS法测定白术炮制前后苍术酮的含量

目的:比较白术药材炮制前后苍术酮的含量变化。方法:采用薄层扫描法比较3批不同产地白术炮制前后苍术酮的峰面积变化。结果:3批不同产地的白术麸炒品中苍术酮的含量均低于生品。结论:炮制后苍术酮的含量有所降低,为白术炮制前后质量控制提供依据。

TLC生物自显影技术在药物筛选中的应用

TLC生物自显影是一种将薄层色谱分离和生物活性测定相结合的药物筛选方法,具有操作简单、耗费低、灵敏度和专属性高等优点,是一种快速测定生物活性的方法。可用于对具有抗菌/真菌,抑制胆碱酯酶以及清除自由基和抗氧化等活性的天然产物的筛选,可实现活性指导的提取分离。利用TLC生物自显影,可测定单体活性化合物的最小抑制浓度(MID),通过与对照品的MID值比较,能够推测该化合物活性的强弱。此外,利用TLC生物自显影定性及定量的特征,还可对食物、水样及动物组织中抗生素残留量进行监测。现综述TLC生物自显影技术及其在天然产物筛选中的应用,同时对其优点及不足也进行了总结。

灵芝胶囊中腺苷、腺嘌呤的TLC鉴别及腺苷的HPLC法含量测定

目的:建立灵芝胶囊中腺苷及腺嘌呤的TLC鉴别方法及腺苷的HPLC含量测定方法。方法:TLC法,采用硅胶GF_(254)薄层板,以氯仿-乙酸乙酯-异丙醇-水(8:3:1.5:0.5)为展开剂,紫外光灯(254 nm)检视。HPLC法,采用Shiseido C_(18)色谱柱(4.6 mm×250 mn,5μm),以甲醇-0.01mol·L^(-1)磷酸二氢钾(10:90)为流动相,流速1.0 mL·min^(-1),检测波长:254 nm,柱温26℃。结果:样品中腺苷及腺嘌呤的TLC分离良好;HPLC法测定,腺苷进样量在2.08 ng～2.08μg范围内,与峰面积积分值呈良好的线性关系,(r=0.9999),最低检出量为2.08 ng,平均回收率为100.3%(n=9)。结论:TLC法及HPLC法重复性好,且简便、易行,可用于灵芝胶囊剂叶腺苷类成分的检测。

红檵木花色素TLC分离与定性研究

通过对红檵木花色素的硅胶H和硅胶G薄板层析(TLC)分离研究,得到其TLC最佳展层剂(体积比)是:水:乙醇:正丁醇:乙酸乙酯=12:8:24:36。以最佳展层剂展开花中的色素,得到颜色及相应Rf值完全一致的7个色斑,反映该花色素含七种色素单体成分。对色素母液和各色素单体成分样液的200～800nm扫描测定表明,色素母液的紫外特征吸收峰是277nm,各单体成分的特征吸吸收峰分别为:272、278、277、270、273、274、271nm,推论认为277nm单体成分(成分③)在花中含量最大。结合色素母液及色素单体样液的甲醇和乙醇样液扫描分析与花青素、黄酮类及蒽醌类特征显色反应研究结果,基本确认红檵木花中的色素主要成分属二氢黄酮醇类。