By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of re...By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...展开更多
目的构建大肠杆菌双杂交系统的诱饵重组质粒。方法PCR扩增大鼠AMP激活的蛋白激酶α2(AMP-ac-tivated prote in k inaseα2,AMPKα2)蛋白编码区cDNA,与大肠杆菌双杂交表达载体pBT构建融合蛋白表达质粒pBT-AMPKα2。经酶切和测序鉴定后,将...目的构建大肠杆菌双杂交系统的诱饵重组质粒。方法PCR扩增大鼠AMP激活的蛋白激酶α2(AMP-ac-tivated prote in k inaseα2,AMPKα2)蛋白编码区cDNA,与大肠杆菌双杂交表达载体pBT构建融合蛋白表达质粒pBT-AMPKα2。经酶切和测序鉴定后,将pBT-AMPKα2转化XL-1 B lue MRF’大肠杆菌报告菌株,检测诱饵融合蛋白的表达。并用pBT-AMPKα2和pTRG空载体共转化XL-1 B lue MRF’菌株,通过3-氨基-1,2,4-三唑(3-am ino-1,2,4-triazole,3-AT)选择性筛选平板检测诱饵融合蛋白的自激活作用。结果酶切和测序结果表明AMPKα2蛋白编码序列成功克隆入pBT载体,融合区阅读框正确。诱饵重组质粒pBT-AMPKα2能表达λcI/AMPKα2融合蛋白。转化有pBT-AMPKα2和pTRG空载体的XL-1 B lue MRF’大肠杆菌报告菌株在3-AT选择性筛选平板上不生长,而在no 3-AT非选择性筛选平板上生长良好,表明pBT-AMPKα2重组质粒能在大肠杆菌细胞中正确表达且无自激活作用。结论构建的pBT-AMPKα2诱饵重组质粒可用于下一阶段的cDNA文库筛选。展开更多
构建和转化神经营养素3(neurotrophin 3,NT3)的酵母诱饵重组质粒,为通过酵母双杂交研究NT3的功能及作用机制奠定基础。用PCR扩增NT3基因cDNA中编码完整开放读框的基因片段;将该基因片段与pLexA载体定向重组;用酶切和测序鉴定重组质粒;...构建和转化神经营养素3(neurotrophin 3,NT3)的酵母诱饵重组质粒,为通过酵母双杂交研究NT3的功能及作用机制奠定基础。用PCR扩增NT3基因cDNA中编码完整开放读框的基因片段;将该基因片段与pLexA载体定向重组;用酶切和测序鉴定重组质粒;将核苷酸序列正确的重组质粒转化入EGY48[p8op LacZ]酵母菌株。结果成功构建pLexA NT3重组质粒。转化有重组质粒和pLexA空载体的二种EGY48[p8op LacZ]酵母都能在SD Gal Raf His Ura培养基中长成白色菌落(同时,转化pLexA pos阳性对照质粒的酵母菌在相同条件下长成蓝色菌落),但都不能在SD His Leu Ura培养基中生长,在SD His Ura液中培养16h后,OD600均值均为0.8±0.1。这表明,重组质粒表达的融合蛋白没有激活LEU2和lacZ酵母报告基因表达的活性,也没有酵母毒性作用。因此,构建的诱饵重组质粒可以用于下一阶段的人胎脑cDNA文库筛选。展开更多
基金supported by grants from National Natural Sciences Foundation of China(No.30672227,30600668)"973"Program of China(No.2009CB521800)Joint Research Fund for Overseas Chinese,Hong Kong and Macao Young Scholars(No.30628029)
文摘By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...
文摘目的构建大肠杆菌双杂交系统的诱饵重组质粒。方法PCR扩增大鼠AMP激活的蛋白激酶α2(AMP-ac-tivated prote in k inaseα2,AMPKα2)蛋白编码区cDNA,与大肠杆菌双杂交表达载体pBT构建融合蛋白表达质粒pBT-AMPKα2。经酶切和测序鉴定后,将pBT-AMPKα2转化XL-1 B lue MRF’大肠杆菌报告菌株,检测诱饵融合蛋白的表达。并用pBT-AMPKα2和pTRG空载体共转化XL-1 B lue MRF’菌株,通过3-氨基-1,2,4-三唑(3-am ino-1,2,4-triazole,3-AT)选择性筛选平板检测诱饵融合蛋白的自激活作用。结果酶切和测序结果表明AMPKα2蛋白编码序列成功克隆入pBT载体,融合区阅读框正确。诱饵重组质粒pBT-AMPKα2能表达λcI/AMPKα2融合蛋白。转化有pBT-AMPKα2和pTRG空载体的XL-1 B lue MRF’大肠杆菌报告菌株在3-AT选择性筛选平板上不生长,而在no 3-AT非选择性筛选平板上生长良好,表明pBT-AMPKα2重组质粒能在大肠杆菌细胞中正确表达且无自激活作用。结论构建的pBT-AMPKα2诱饵重组质粒可用于下一阶段的cDNA文库筛选。
文摘构建和转化神经营养素3(neurotrophin 3,NT3)的酵母诱饵重组质粒,为通过酵母双杂交研究NT3的功能及作用机制奠定基础。用PCR扩增NT3基因cDNA中编码完整开放读框的基因片段;将该基因片段与pLexA载体定向重组;用酶切和测序鉴定重组质粒;将核苷酸序列正确的重组质粒转化入EGY48[p8op LacZ]酵母菌株。结果成功构建pLexA NT3重组质粒。转化有重组质粒和pLexA空载体的二种EGY48[p8op LacZ]酵母都能在SD Gal Raf His Ura培养基中长成白色菌落(同时,转化pLexA pos阳性对照质粒的酵母菌在相同条件下长成蓝色菌落),但都不能在SD His Leu Ura培养基中生长,在SD His Ura液中培养16h后,OD600均值均为0.8±0.1。这表明,重组质粒表达的融合蛋白没有激活LEU2和lacZ酵母报告基因表达的活性,也没有酵母毒性作用。因此,构建的诱饵重组质粒可以用于下一阶段的人胎脑cDNA文库筛选。