Effect of Feeding Different Levels of Dietary Protein and Addition of Baker＇s Yeast （Saccharomyces cerevisiae） on Productive Parameters of Awassi Lambs

Forty eight individually fed Awassi male lambs were used in factorial experiment to investigate their responses to feeding concentrate diets containing three levels of dietary crude protein （CP）, each was offered without or with baker＇s yeast （SC） at rate of 0.5% （on dry matter （DM） basis）. Concentrates were offered at rate of 3% of live body weight with free choice of barley straw. Results revealed that higher （P 〈 0.05） digestible dry matter （DDM） and digestible organic matter （DOM） intakes were achieved due to feeding medium level of CP and to the addition of SC. Addition of SC improved （P 〈 0.05） gain, lambs fed medium and high levels gained higher （P 〈 0.05） than those fed the low level of dietary CP. Feed conversion ratio （FCR） based on DM and organic matter （OM） intakes was not significantly affected by level of dietary CP or addition of yeast. Even though, less amount of N required per unit of gain was achieved with low and medium as compared to high levels. Higher DM, nitrogen free extract （NFE） and hemicellulose （P 〈 0.05）, OM, CP, crude fiber （CF） and cellulose （P 〈 0.01） digestibilities were achieved in lambs fed the medium level of CP, whereas, no significant effect was observed on ether extract （EE）, neutral detergent fiber （NDF） and acid detergent fiber （ADF） digestibilities. Results also revealed that digestibility of almost all nutrients was improved with different extent due to addition of SC. Effect of interaction between levels of dietary CP and addition of SC referred to the preferability of addition of SC with medium level of dietary protein.

Effect of Using Baker＇s Yeast and Exogenous Digestive Enzymes as Growth Promoters on Growth, Feed Utilization and Hematological Indices of Nile tilapia, Oreochromis niloticus Fingerlings

The present study was conducted to evaluate the effect of baker yeast, Saccharomyces cerevisiae （SC） and exogenous digestive enzymes （pepsin, papain and a-amylase, EDE） dietary supplementation on growth performance, feed utilization and hematological indices of Nile tilapia, Oreochromis niloticus fingerlings. A total of 630 Nile tilapia fingerlings with an average body weight of 26.4 ± 0.2 g were divided in the seven experimental net-pen treatments （three replicates each）. The experiment was conducted for 119 days. Seven isonitrogenous （26.50%） digestible protein and isocaloric （13.40 MJ kgl） digestible energy experimental diets were formulated. The control diet had no SC and EDE added. Diets 2-3 each contained SC at levels of 2 and 4 g 100 g diet-t, respectively, while diets 4-5 each contained EDE at levels of （0.64, 1.28, 0.16） and （1.28, 2.56, 0.32） g 100 gdiefI of pepsin, papain and a-amylase, respectively. Diet 6 contained mixture of SC and EDE at levels of 1 g yeast and 0.32, 0.64, 0.08 g of pepsin, papain and a-amylase, respectively 100 gdiet1 and diet D7 contained 2 g yeast and 0.64, 1.28, 0.16 g of pepsin, papain and a-amylase, respectively 100 g dietl. Growth performance and feed utilization efficiency of Nile tilapia were significantly （P 〈 0.05） higher in all treatments receiving SC and/or EDE supplemented-diets than the control diet which suggests that the addition of SC and EDE enhanced the growth performance. Red blood cells counts, hematocrit and hemoglobin were significantly （P 〈 0.05） highest in all treatments receiving mixture of SC and EDE supplemented-diets （D6 ＋ D7）. The same trend was observed for total plasma protein and total plasma globulin levels. The results of present study suggested that Nile tilapia fingerlings fed diets containing the mixture of I g yeast, SC and 0.32, 0.64, 0.08 g of pepsin, papain and a-amylase, respectively 100 gdiet^-1, for 119 days had enhanced growth performance, diet utilization efficiency and hematological indices.

Effect of β-Glucan (Angel Yeast) Compared to a Placebo on Cold and Flu Incidence and Symptoms in an Adult Population—A Double Blind, Randomised Controlled Trial

Background: 1-3, 1-6 β-glucan derived from Baker’s yeast (Saccharomyces cerevisiae) has been widely studied for its immune stimulatory capabilities and safety. Previous studies found β-glucan to have efficacy at reducing incidence of URTIs as well as being a low risk for negative side effects. The current study aimed to examine the effects of yeast β-glucan (Angel Yeast) on cold and flu incidences and symptoms in healthy adults. Methods: Two hundred and thirty-one males and females aged 18 to 65 years old supplemented with either β-glucan or a placebo for 3-months. Participants completed a general health questionnaire every 4 weeks and in addition, if participants experienced any cold or flu symptoms, these were recorded daily (along with severity) until resolved or up to 2 weeks. Results: Supplementation with β-glucan reduced the self-reported severity of sore throats and improved sleep quality compared to the placebo group. Conclusions: Yeast β-glucan supplementation appears to be able to help reduce certain symptoms experienced during a cold or flu episode and is safe and well tolerated.

The setereoselective preparation ofβ-keto esters using baker's yeast in the presence of rice wine koji

By the addition of rice wine koji, enhancement of the reactivity was observed for the baker＇s yeast reduction of β-keto esters into （S）-β-hydroxy esters with high enantiomeric purity （73-98%）.

Reaction Characteristics of Asymmetric Synthesis of (2S,5S)-2,5-Hexanediol Catalyzed with Baker’s Yeast Number 6

Baker’s yeast number 6 was selected by screening. It showed good catalytic activity and enantioselec-tivity for asymmetric reduction of 2,5-hexanedione to produce (2S,5S)-2,5-hexanediol. Gas chromatography-mass spectrometry (GC-MS) revealed that the intermediate was (S)-5-hydroxyhexane-2-one. Reduction of 2,5-hexanedione proceeded in a two-step reaction. The hydroxyketone was initially formed, and this intermediate was further re-duced to the diol. Factors influencing the product yield and the enantiomeric excess of the reduction of 2,5-hexandione catalyzed by baker’s yeast number 6 were investigated. Higher concentration (≤100 mmol·L-1) of 2,5-hexandione did not influence 5-hydroxyhexane-2-one production, but 2,5-hexanediol production was inhibited by excess accumulation (>30 mmol·L-1) of intermediate. The optimal conditions were glucose as the co-substrate at an initial glucose concentration of 20 g·L-1, 34°C, pH 7.0 and cell concentration 60 g·L-1 (cell dry mass). Under the optimal condition and an initial substrate concentration of 30 mmol·L-1, the yield of 2,5-hexandiol was 78.7% and the enantiomeric excess of (2S,5S)-2,5-hexandiol was 94.4% for 24-h reduction.

Baker's Yeast Mediated Reduction of Optically Active Diketone

Baker's Yeast mediated reduction of optically active diketone has been described.

Baker's yeast mediated reduction of substituted acenaphthenequinones: Regio-and enantioselective preparation of mono-hydroxyacenaphthenones

Baker's yeast mediated reduction of acenaphthenequinone within 4-10 h afforded mono-hydroxyacenaphthenone mainly with low enantioselectivity, the substrate and mono-hydroxyacenaphthenone product almost converted to dihydroxyacenaphthene after 48 h. By control of the reaction time and in the presence of DMF as co-solvent, the reduction of 6-substituted acenaphthenequinones under vigorous agitation afforded the corresponding 2-hydroxyacenaphthenones in 24-84% yields with 10-93% ee.

Loose Body in the Knee Joint: A Case Report

Background: Loose bodies (LBs) within the knee joint are commonly encountered during clinical practice and are frequently observed during knee arthroscopy. The primary treatment involves the removal of loose bodies;however, their complete eradication is often challenging and may not address underlying diseases, leading to persistent symptoms and the risk of new loose body formation. Aim: This case report aims to present the findings and surgical management of a 52-year-old male with an unusually large osseous loose body in the knee joint and associated pathologies. Case Presentation: The patient, a 52-year-old male, experienced recurrent episodes of severe, sudden, and painful locking of the knee joint, leading to difficulties moving. A plain MRI study was conducted to evaluate the condition of the knee joint, which revealed various degenerative changes and the presence of a loose body. Subsequently, an arthroscopic examination was performed under general anesthesia, uncovering the presence of an abnormally large loose body, as well as other pathologies including chondropathy, meniscal degeneration, and Baker’s cyst. Conclusion: Loose bodies (LBs) in the knee joint pose significant challenges and may lead to debilitating symptoms. Timely diagnosis and appropriate surgical intervention are crucial for symptom relief and the prevention of further joint damage as arthroscopic excision. Comprehensive imaging has a vital role in guiding treatment decisions and optimizing patient outcomes. In this case, the removal of the loose body improved patient outcomes and helped prevent potential joint complications.

关节镜微创手术治疗膝骨性关节病伴Baker’s囊肿的价值

目的探讨关节镜微创技术对膝骨性关节病伴Baker’s囊肿的治疗价值。方法采用局麻关节镜微创技术治疗膝骨性关节病合并Baker’s囊肿34例。男性14例，女性20例，年龄53～66岁，平均61岁。术前行膝关节MRI检查28例，CT扫描14例，B超12例，Baker’S囊肿造影5例。影像学检查显示软骨退变26例，半月板损伤11例，囊肿与后关节囊相通28例，不通6例。关节镜下同期行膝关节清理与囊肿摘除术22例，分期囊肿摘除术12例。局麻关节镜下检查、半月板和软骨损伤创面清理，滑膜切削，然后改为俯卧位关节镜下Baker’s囊肿摘除术。将囊壁与周围组织剥离，刨削器吸引下清除囊壁组织，再用等离子刀冷凝止血，创面组织皱缩消融术。结果手术34例，术后获得随访31例，随访时间9～32个月，平均16个月。术后29例膝关节疼痛、肿胀、绞锁症状和Baker’s囊肿解除，下蹲活动和行走功能恢复正常，自觉疗效满意。无血管、神经损伤、伤口感染和下肢血栓形成。术后Baker’s囊肿复发2例为严重骨关节炎患者。结论关节镜下摘除Baker’s囊肿同期或分期膝关节清理术治疗膝骨性关节病伴Baker’s囊肿方法可行，且创伤小，有利于术后功能恢复，疗效满意。

高频超声结合宽景成像技术对Baker’s囊肿破裂的诊断

目的分析Baker’s囊肿破裂的高频超声及宽景成像声像图表现及其诊断价值。方法回顾分析10例Baker’s囊肿破裂的高频超声及宽景成像声像图,并与术后的病理结果进行对照。结果 10例Baker’s囊肿破裂中,8例破入腓肠肌与比目鱼肌之间,2例破入腓肠肌表层、筋膜深方;1例合并小腿筋膜室综合征,3例合并小腿深静脉血栓。均与手术病理结果相符。结论高频超声结合宽景成像技术可对Baker’s囊肿破裂进行明确诊断。

The Comparison and Contrast of Skopostheorie and Baker's Equivalence Translation Approach

Equivalence can be said to be the central issue in translation although its different approaches have caused heated controversy.However,as early as the 1970s,the Skopostheorie emerged in Germany,and it seemed to challenge the equivalence theories.Although Nida and some linguistics raised some negative problems of this theory,it still rapidly became famous around the world,This article will mainly compare and analyse Skopostheorie and Baker's translation equivalence approach.First,it will give a simple overview of these two approaches.Second,it will discuss the relevant strengths and weaknesses of these two approaches in relation to their usage.Finally,it will discuss combining these two approaches in the same translation.

应用固定化Baker’s酵母还原醌类化合物的研究

苯醌、萘醌和蒽醌类化合物在水溶液中能被固定化Baker’s酵母还原。本文研究了影响固定化Baker’s酵母还原活性、醌类化合物还原速度的因素及还原产物的分离和稳定性。

A rapid absorbance-based growth assay to screen the toxicity of oligomer Aβ42 and protect against cell death in yeast

Multiple lines of evidence show that soluble oligomer forms of amyloidβprotein(Aβ42)are the most neurotoxic species in the brain and correlates with the degree of neuronal loss and cognitive deficit in Alzheimer’s disease.Although many studies have used mammalian cells to investigate oligomer Aβ42 toxicity,the use of more simple eukaryotic cellular systems offers advantages for large-scale screening studies.We have previously established and validated budding yeast,Saccharomyces cerevisiae to be a simple and a robust model to study the toxicity of Aβ.Using colony counting based methods,oligomeric Aβ42 was shown to induce dose-dependent cell death in yeast.We have adapted this method for high throughput screening by developing an absorbance-based growth assay.We further validated the assay with treatments previously shown to protect oligomer Aβ42 induced cell death in mammalian and yeast cells.This assay offers a platform for studying underlying mechanisms of oligomer Aβ42 induced cell death using gene deletion/overexpression libraries and developing novel agents that alleviate Aβ42 induced cell death.

Helicobacter is preserved in yeast vacuoles! Does Koch's postulates confirm it?

The manuscript titled "Vacuoles of Candida yeast behave as a specialized niche for Helicobacter pylori(H.pylori)" not only has not been prepared in a scientific manner but the methodology used was not adequate, and therefore the conclusion reached was not correct. First of all, "yeast" is a broad terminology covering a great number of genera and species of unicellular micro-organisms. The authors should have defined the organism with its binary scientific name. This measure would allow experiment reproduction by the scientific community. Moreover, the criteria established by Robert Koch to identify a specific microorganism or pathogen was not adopted in the methodology used. Regarding the methodology applied, use of the chicken eggyolk(Ig Y) antibody and PCR of the apparently tainted yeast population to prove H. pylori existence in the yeast vacuoles might be main factors for their wrong conclusions. Bacterial tropism toward yeast extract is a known phenomenon, and yeast extract is one of the main ingredients in culture media. Their internalization through phagocytosis or similar pathways does not seem possible or practical because of the thick and cellulosic yeast wall. While the small size of yeast cells does not support their ability in harboring several H. pylori, other observations such as inefficiency of antifungal therapy as anti-Helicobacter therapy strongly reject the conclusion reached by the above-mentioned article.

Yeast One-hybrid System Used to Identify the Binding Proteins for Rat Glutathione S-transferase P Enhancer I

Objective To detect the trans-factors specifically binding to the strong enhancerelement (GPEI) in the upstream of rat glutathione S-transferase P (GST-P) gene. MethodsYeast one-hybrid system was used to screen rat lung MATCHMAKER cDNA library toidentify potential trans-factors that can interact with core sequence of GPEI(cGPEI).Electrophoresis mobility shift assay (EMSA) was used to analyze the binding of trans-factors to cGPEI. Results cDNA fragments coding for the C-terminal part of thetranscription factor c-Jun and rat adenine nucleotide translocator (ANT) were isolated. Thebinding of c-Jun and ANT to GPEI core sequence were confirmed. Conclusions Rat c-juntranscriptional factor and ANT may interact with cGPEI. They could play an important rolein the induced expression of GST-P gene.

Transcriptional activation function of hepatitis B virus Pre S1 protein in yeast

OBJECTIVE: To explore the feasibility of cloning of the hepatocyte receptor interacting with the Pre Slprotein of HBV by two-hybrid system.METHODS: Yeast expression plasmids encoding fusion proteins of full length or portions of Pre Sl ofHBV and DNA binding domain of yeast protein GAL4 were constructed and used to transform yeastreporter strain SFY526. Reporter gene product β-galactosidase activity was assayed as a measure oftranscriptional activation in yeast, Mammalian expression plasmid encoding fusion proteins of full lengthPre Sl and DNA binding domain of GAL4 was constructed and used to cotransfect hepatoma cell lineHuh-7 together with CAT reporter plasmid. Cell extracts were assayed for CAT activity by thin-layerchromatography.RESULTS: The fusion proteins of full length Pre Sl protein and GAL4 DNA binding domain presentedtranscriptional activation function in yeast. The transcription activating sequence was localized to the 21 to47 amino acids of Pre Sl protein. Fusion proteins of full length Pre Sl and GAL4 DNA binding domaindid not show transcriptional activation function in mammalian cells.CONCLUSIONS: The transcription activating sequence of HBV Pre Sl protein in yeast overlaps thehepatocyte receptor binding site. The transcriptional activation function of HBV Pre Sl protein in yeastmay prevent researchers from using yeast two-hybrid system to clone HBV receptor interacting with Pre Slprotein. However, the Pre Sl protein does not show transcriptional activation function in mammaliancells. Mammalian two-hybrid system may be a practical method to clone the HBV hepatocyte receptorinteracting with Pre Sl protein.

Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast

Cohesin is an evolutionary conserved complex that controls chromosome segregation during mitosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of the Smc1-Smc3-Scc1 cohesin ring, is phosphorylated on S/T-Q residues. This event depended on the Mec1 checkpoint kinase as well as on cell cycle arrest triggered by the DNA damage checkpoint network. This phosphorylation event also took place during mitosis of an unperturbed cell cycle. The present finding that S. cerevisiae Scc3 is phosphorylated during mitosis represents a potentially important new regulatory step in cohesin’s mitotic functions.

Isolation and Partial Evaluation of a Potential Indigenous Yeast Strain <i>Pichia kudriavzevii</i>from a Traditional Rice Beer—“Gora” Prepared by the Koloi Tribes of Tripura

As per tradition from hundreds of years the Koloi tribes of Tripura are preparing “Gora”-therice based fermented beer which is very good in taste and aroma applying their traditional indigenous brewing techniques. In this study, an attempt has been made to identify the indigenous yeast which is the main causative agent for fermentation and to investigate its fermentation ability with an industrial Saccharomyces cerevisiaes train. After investigation based on culture dependent phenotypic characteristics like-staining and biochemical characterization, primarily the responsible yeast species was determined as Pichia kudriavzevii and further confirmed followed by 18S rRNA ribotyping and the sequences was deposited at Gene bank and NCBI bearing specific accession number. In the comparative analysis, it has been found a significant similarities in all aspects of nutritional and alcohol percentages with the industrial strain in laboratory condition. The alcohol percentage in the rice beer “Gora” measured 6.40 ± 0.008% v/v. The study may be the first scientific investigation of its kind about this indigenous yeast strain isolated from “Gora” of this Indo-Burma biodiversity region and may provide sufficient background and potentiality for promoting these kinds of indigenous alcoholic beverages for small scale commercialization to strengthen the rural livelihood as well as to maintain immaterial cultural heritage.

Efficacy of Xiaojin Capsules Combined with Selenium Yeast Capsules on 120 Cases of Hashimoto's Thyroiditis with Abnormal Thyroid Function

[Objectives]To explore the efficacy of Xiaojin Capsules combined with Selenium Yeast Capsules in the treatment of Hashimoto's thyroiditis(HT)with abnormal thyroid function.[Methods]A total of 180 HT patients who were treated in the First Affiliated Hospital of Henan University and Henan Provincial People's Hospital from December 2020 to December 2021 were selected as the research subjects and randomly divided into the observation group(n=120)and the control group(n=60)in at a tratio of 2∶1 ratio according to their visiting time.The observation group was treated with Xiaojin Capsules(oral,5 capsules each time,twice a day for 12 weeks)and Selenium Yeast Capsules(oral,1 capsule each time,twice a day for 12 weeks).The control group was treated with only Selenium Yeast Capsules(oral,1 capsule each time,twice a day for 12 weeks).[Results]The difference in the levels of thyroid peroxidase antibody(TPO-Ab),thyroglobulin antibody(TGAb),serum free triiodothyronine(FT_(3)),serum free thyroxine(FT_(4)),and thyroid stimulating hormone(TSH)between the observation group and control group was statistically significant(P<0.05).The diameters of thyroid between the observation group and the control group before,during and after treatment were significantly different(P<0.05).The total effective rate of the observation group was 82.5%(99/120),which was significantly higher than 56.67%(34/60)in the control group,and the difference was statistically significant(P<0.05).The cure rate(22.50%)in the observation group was significantly higher than the control group(3.33%),and the difference was statistically significant(P<0.01).The ineffective rate of the observation group was 17.50%,which was significantly lower than that of the control group(40.00%),and the difference was statistically significant(P<0.01).The markedly effective rate of the observation group was 40.83%,which was significantly higher than that of the control group(31.67%),and the difference was statistically significant(P<0.05).The effective rate in the observation group was 191%,and the effective rate in the control group was 21.67%,and the difference was not statistically significant(P>0.05).[Conclusions]Xiaojin Capsules combined with Selenium Yeast Capsules in the treatment of HT can eliminate or alleviate the clinical symptoms and signs,significantly reduce the levels of TPO-Ab and TGAb in serum,restore thyroid function,improve thyroid shape and structure in the treatment of HT,and the clinical effect is satisfactory.

Recovery of Residual Brewer’s Yeast by Electroactivation

Yeasts resulting from the brewing process (RBY) are a valuable by-product, with an important content of minerals, vitamins and, especially, proteins. The purpose of the research was the electroactivation of RBY and the simultaneous obtaining of two products—protein concentrates and hydrolyzed protein from residual brewer’s yeast. Electroactivation is a non-residual process, without the use of chemical reagents and relatively inexpensive. The variation of the electroactivation conditions allowed the separation of 90% - 94% of the proteins in the form of protein concentrates. During the process, it is attested to increase the pH value and decrease the redox potential, which characterizes the multiple redox processes that take place in the cathode cell, including sedimentation at the isoelectric point. The presence of albumin in the protein fractions of RBY allows the formation of protein complexes with calcium, attributing a higher biological value to the obtained products.