尿液TWIST1基因甲基化检测在膀胱癌术后监测中的价值

目的探索尿液TWIST1基因甲基化检测在膀胱癌术后复发监测中的预测作用。方法应用TWIST1基因甲基化试剂盒对接受经尿道膀胱肿瘤切除术治疗的51例非肌层浸润性膀胱癌患者进行随访复查,对比膀胱镜检查评估其价值,并对患者临床病理资料以及术后复发情况进行统计学分析。结果16例患者尿液TWIST1基因甲基化检测阳性,以同期膀胱镜检为标准,灵敏度达78.9%、特异度96.8%、阳性预测值93.7%、阴性预测值88.5%。结论尿液TWIST1基因甲基化检测可作为膀胱肿瘤患者电切术后复发的有效监测手段。

基于Super-Twisting滑模S面的无人机路径跟踪控制

针对小型固定翼无人机在执行任务时跟踪精度低以及容易受外界风影响的问题,设计基于Super-Twisting滑模S面(STSM S-Plane)的路径跟踪控制器,同时采用内外双环控制模式。外环即速度环采用Super-Twisting滑模控制,内环即姿态环采用S面控制。考虑到S面控制求导易导致积分爆炸的问题引入了二阶微分器,并对外界风组成进行建模研究。最后通过空间特殊曲线来验证所设计算法的控制性能。仿真结果表明,所设计的算法可以实现固定翼无人机对期望路径的精确跟踪,并具有良好的鲁棒性和抗干扰性能。

基于改进型Super-twisting的双馈感应发电机直接功率控制

为提高双馈感应发电机(DFIG)直接功率控制的鲁棒性和抗干扰能力,提出一种改进型超螺旋滑模(STSMC)的直接功率控制(DPC)。首先,建立参数摄动下的DFIG最大功率捕获模型和定子磁场定向的双馈感应发电机模型;然后,将非奇异快速终端滑模面(NFTSM)与一种改进型超螺旋(Super-twisting)优化算法结合,设计DFIG直接功率控制器(IST-NFTSMC)。其中所设计的非奇异滑模面能有效避免传统滑模控制(SMC)的奇异现象,且改进超螺旋控制律能有效削弱NFTSMC抖振,提高功率控制精度;最后,通过仿真和实验,与PI控制和传统SMC控制相比,该控制能削弱DFIG功率与电流抖振问题,加快其收敛速度,提高DFIG直接功率控制稳态精度。

Twisted Yangian Y(sp_(2))的单权模

讨论了Twisted Yangian Y(sp_(N))的权模问题,构造了Y(sp_(2))的一类无限维单权模,分类了具有一个一维权空间的Y(sp_(2))单权模.

TFF3依赖TWIST1上调TRIB3促结直肠癌转移的机制

目的探讨TFF3基因过表达对结直肠癌HT29细胞增殖、迁移和侵袭能力的影响,以及与癌转移相关的机制。方法构建TFF3过表达的HT29细胞系,qRT-PCR和Western blot法检测TFF3、TWIST1/TRIB3信号通路及上皮间质转化过程中相关mRNA和蛋白的表达,CCK-8法检测HT29细胞增殖能力,细胞划痕实验和Transwell实验评估细胞迁移和侵袭能力,透射电子显微镜观察TFF3过表达前后细胞形态的变化。结果pIRES2-TFF3质粒转染HT29细胞后,相较于对照组,TFF3 mRNA和蛋白表达水平显著升高(P<0.01),细胞增殖、迁移和侵袭能力显著增强(P<0.01),且TWIST1、TRIB3、Vimentin、Snail的表达也显著上升,而E-cadherin的表达则明显下降(P<0.05)。TFF3过表达后的HT29细胞出现了多种变化,如细胞间连接减少、绒毛脱落、伪足形成,以及形态上梭形细胞增多等。结论TFF3过表达可能通过Twist1/TRIB3信号通路促进结直肠癌细胞的EMT过程,从而提高其转移潜力,加速癌症的恶性进展。

Twisted Yangian Y(sp^(2))的一类单权模

研究了Twisted Yangian Y(g_(N))的权模问题,构造了Y(sp^(2))的一类权空间维数均为2的无限维权模,给出了该权模不可约的充分条件.

Twist2表达与卵巢癌脉管浸润及预后的相关研究

目的:分析Twist2与卵巢癌患者的预后及脉管浸润之间的关系,探索Twist2在卵巢癌脉管浸润中的作用及机制。方法:利用KM plotter分析卵巢癌患者中Twist2 mRNA的表达与卵巢癌总生存率(OS)、无进展生存率(PFS)、肿瘤进展后生存率(PPS)的相关性。利用癌症基因组学数据库cBioportal分析卵巢癌患者中Twist2与脉管浸润的相关性。体外实验:采用Transwell检测Twist2对卵巢癌CAOV3细胞[空白组、阴性对照组(siNC组)、siTwist2组]侵袭、迁移能力的影响,并利用实时荧光定量多聚核苷酸链式反应(realtime-PCR)及蛋白免疫印迹(Western blot)检测分别从RNA水平及蛋白水平检测下调Twist2表达后CAOV3细胞中Twist2、血管内皮生长因子C(VEGFC)表达的变化。结果:①KM plotter在线数据分析结果示,Twist2高表达的卵巢癌患者与不良预后有关,OS(HR 1.24,95%CI 1.01~1.52)、PFS(HR 1.39,95%CI 1.14~1.70)及PPS(HR 1.37,95%CI 1.08~1.74)差异均有统计学意义(P<0.05);cBioportal分析示,Twist2 mRNA表达与卵巢癌血管浸润(r=0.93,P=0.001)及淋巴管浸润(r=0.89,P=0.009)均呈正相关。②体外实验Transwell检测示,与空白组和siNC组比较,siTwist2组卵巢癌细胞的侵袭及迁移能力显著下降(P<0.05)。Realtime-PCR及Western blot检测示,与空白组和siNC组比较,siTwist2组中Twist2 mRNA及蛋白、VEGFC mRNA及蛋白的表达均明显下降(P<0.05)。结论:卵巢癌中Twist2的表达与肿瘤预后及脉管浸润密切相关,下调Twist2后,细胞迁移及侵袭穿透的细胞数明显减少、VEGFC表达降低,Twist2可能通过VEGFC来诱导肿瘤细胞侵袭转移参与肿瘤脉管浸润,Twist2可能是未来卵巢癌评估预后及靶向治疗的指标之一。

TWIST1通过激活自噬促进肺动脉高压大鼠模型肺血管重塑作用及机制的实验研究

目的探讨Twist相关蛋白1(Twist-related protein 1,TWIST1)对野百合碱(Monocrotaline,MCT)诱导的肺动脉高压(pulmonary arterial hypertension,PAH)大鼠肺血管重塑的影响及机制。方法将50只健康雄性SD大鼠,随机分为5组,即对照组、模型组、二甲基亚砜(Dimethyl sulfoxide,DMSO)溶剂处理的模型组、TWIST1抑制剂Harmine组、自噬抑制剂羟氯喹组。检测右心室收缩压(RVSP),右心室肥厚指数(RVHI)和内膜厚度百分比(MT%)以评估大鼠PAH的发展。采用免疫印迹法检测TWIST1,自噬相关蛋白LC3B以及RND3的蛋白质水平。结果与对照组比较,MCT诱导的PAH大鼠中TWIST1和LC3B表达增加,分别为对照组的2.32±0.22倍和0.87±0.19倍,差异具有统计学意义(t=15.812,11.227,均P<0.001);同时RND3表达下调,为对照组的0.32±0.07倍,差异具有统计学意义(t=-13.003,P<0.001)。给与TWIST1抑制剂Harmine或自噬抑制剂羟氯喹可显著抑制MCT诱导的自噬激活和RND3表达下调,同时降低了MCT诱导的PAH大鼠的RVSP,RVHI和MT%,差异具有统计学意义(t=-24.277~16.636,均P<0.001)。结论TWIST1通过诱导自噬激活促进肺血管重塑,促进PAH的发生发展。

Visualizing the Local Twist Angle Variation within and between Domains of Twisted Bilayer Graphene

Moirésuperlattices in twisted two-dimensional materials have emerged as ideal platforms for engineering quantum phenomena,which are highly sensitive to twist angles,including both the global value and the spatial inhomogeneity.However,only a few methods provide spatial-resolved information for characterizing local twist angle distribution.

Valley-dependent transport in a mescoscopic twisted bilayer graphene device

We study the valley-dependent electron transport in a four-terminal mesoscopic device of the two monolayer graphene nanoribbons vertically stacked together, where the intersection forms a bilayer graphene lattice with a controllable twist angle. Using a tight-binding lattice model, we show that the longitudinal and transverse conductances exhibit significant valley polarization in the low energy regime for small twist angles. As the twist angle increases, the valley polarization shifts to the high energy regime. This arises from the regrouping effect of the electron band in the twisted bilayer graphene region. But for relatively large twist angles, no significant valley polarization is observed. These results are consistent with the spectral densities of the twisted bilayer graphene.

Twisted Integration of Complex Oxide Magnetoelectric Heterostructures via Water‑Etching and Transfer Process

Manipulating strain mode and degree that can be applied to epitaxial complex oxide thin films have been a cornerstone of strain engineering.In recent years,lift-off and transfer technology of the epitaxial oxide thin films have been developed that enabled the integration of heterostructures without the limitation of material types and crystal orientations.Moreover,twisted integration would provide a more interesting strategy in artificial magnetoelectric heterostructures.A specific twist angle between the ferroelectric and ferromagnetic oxide layers corresponds to the distinct strain regulation modes in the magnetoelectric coupling process,which could provide some insight in to the physical phenomena.In this work,the La_(0.67)Sr_(0.33)MnO_(3)(001)/0.7Pb(Mg_(1/3)Nb_(2/3))O_(3)-0.3PbTiO_(3)(011)(LSMO/PMN-PT)heterostructures with 45.and 0.twist angles were assembled via water-etching and transfer process.The transferred LSMO films exhibit a fourfold magnetic anisotropy with easy axis along LSMO<110>.A coexistence of uniaxial and fourfold magnetic anisotropy with LSMO[110]easy axis is observed for the 45°Sample by applying a 7.2 kV cm^(−1)electrical field,significantly different from a uniaxial anisotropy with LSMO[100]easy axis for the 0°Sample.The fitting of the ferromagnetic resonance field reveals that the strain coupling generated by the 45°twist angle causes different lattice distortion of LSMO,thereby enhancing both the fourfold and uniaxial anisotropy.This work confirms the twisting degrees of freedom for magnetoelectric coupling and opens opportunities for fabricating artificial magnetoelectric heterostructures.

Wafer-scale 30°twisted bilayer graphene epitaxially grown on Cu_(0.75)Ni_(0.25)(111)

Twisted bilayer graphene(TBG) has been extensively studied because of its novel physical properties and potential application in electronic devices.Here we report the synthesis and characterization of 300 TBG naturally grown on Cu_(0.75)Ni_(0.25)(111) film and investigate the electronic structure by angle-resolved photoemission spectroscopy.Compared with other substrates,our TBG with a wafer scale is acquired with a shorter growth time.The Fermi velocity and energy gap of Dirac cones of TBG are comparable with those of a monolayer on Cu_(0.85)Ni_(0.15)(111).The signature of moré lattices has not been observed in either the low-energy electron diffraction patterns or the Fermi surface map within experimental resolution,possibly due to different Cu and Ni contents in the substrates enhancing the different couplings between the substrate and the first/second layers and hindering the formation of a quasiperiodic structure.

Tubular Heat Enhancement Using Twisted Tape Inserts with Large Holes

Heat augmentation techniques play a vital role in the heating and cooling processes in industries,including solar collectors and many applications that utilize heat exchangers.Several studies are based on inserting fillers inside the tubes to enhance heat transfer.This investigation considered the effects of twisted tapes with large holes on a tubular heat exchanger’s(HX)heat transmission,pressure drop,and thermal boosting factor.In the experimental section,counter-swirl flow generators used twisted tapes with pairs of 1.0 cm-diameter holes and changes in porosity(Rp)at 1.30%and 2.70%.In the experiments,air was utilized as a working fluid in a tube with a circular cross-section.The turbulent flow was considered,with Reynolds numbers(Re)domain from 4800 to 9500,and a boundary condition with a uniform wall heat flux was constructed.The findings expound that when the number of holes rose,the Nusselt number(Nu),the factor of friction(f),and the thermal enhancement factor(η)all increased as well.Additionally,as the friction factor increased,the Nusselt number of the tape-equipped tube was noticeably higher.Additionally,it was discovered that the friction factor was between 70%and 94%lower than the values of the tube without tape,while the(Nu)was between 87%and 97%higher than the conventional tube values.The maximum value ofηis 89%.According to the experimental results,empirical correlations for Nu,f,andηwere also generated.

基于改进Super-Twisting算法和电流预测的PMSM调速控制

针对永磁同步电机传统PI控制器存在响应速度慢、控制精度低、鲁棒性差等问题,该文提出一种改进调速控制方案。首先,将全局快速积分型终端滑模理论与广义Super-Twisting算法相结合,基于Anti-Windup原理设计改进广义Super-Twisting积分终端滑模速度控制器,并采用新型分段指数函数优化控制律;其次,设计改进扩展扰动观测器对系统未知扰动进行前馈补偿;最后,在电流环引入无差拍电流预测控制器,进一步改善系统的动态响应。仿真结果表明,所提出的改进控制方案能够有效提高调速系统的动态控制性能、控制精度和鲁棒性。

Comparison between seismic analysis of twisting and regular 52-story towers considering soil-structure interaction

A dynamic analysis of both twisting and regular towers is carried out to determine the results of considering soil-structure interaction(SSI)on high-rise buildings.In addition,the difference between the seismic performance of using twisting towers over regular ones is investigated.The twisting tower is a simulation of the Evolution Tower(Moscow).The towers’skeletons consist of RC elements and rest on a reinforced concrete piled-raft foundation.The soil model is considered as multi-layered with the same soil properties as the zone chosen for the analysis(New Mansoura City,Egypt).The only difference between both towers is their shape in elevation.The whole system is modelled and analyzed in a single step as one full 3D model,which is known as the direct approach in SSI.All analyses are carried out using finite-element software(Midas GTS NX).Dynamic output responses due to three records of seismic loads are proposed and presented in some graphs.Based on the results,it is concluded that SSI has a considerable effect on the dynamic response of tall buildings mainly because of the foundation flexibility,as it leads to lengthening the vibration period,increasing the story drift and the base shear for both cases.

Linear Functional Equations and Twisted Polynomials

A certain variety of non-switched polynomials provides a uni-figure representation for a wide range of linear functional equations. This is properly adapted for the calculations. We reinterpret from this point of view a number of algorithms.

基于自适应Super-twisting算法的永磁同步电机驱动控制

在永磁同步电机调速系统中,良好的速度控制器要同时兼顾跟踪性能及抗干扰性能,非线性控制具有快速响应及强鲁棒性的优点,适用于速度控制器的设计,但传统滑模控制存在着严重的抖振问题。自适应Super-twisting算法属于二阶滑模,能将相轨迹的状态量以级数的形式收敛到原点,又能在线调整控制器的增益,因此能有效削弱抖振现象。采用自适应Super-twisting算法来设计速度控制器,并通过李雅普诺夫函数给出其稳定性证明。试验结果表明,所设计的控制器具有良好的跟踪性能及抗干扰性能,同时能减小电流的纹波,达到削弱抖振的目的。

Twist2、Snail、TPX2、Aurora-A在喉鳞状细胞癌组织中的表达及与临床病理特征的相关分析

目的:探究扭曲蛋白2(Twist2)、转录因子(Snail)蛋白、Xklp2靶蛋白(TPX2)、极光激酶A(Aurora-A)在喉鳞状细胞癌组织中的表达及与临床病理特征的相关分析。方法:选取2021年1月至2023年7月大冶市人民医院收治的喉鳞状细胞癌66例为研究对象,取患者手术切除癌组织和癌旁正常组织分为喉鳞状细胞癌组与癌旁组织组。比较两组Twist2、Snail、TPX2、Aurora-A表达差异,通过ROC曲线分析Twist2、Snail、TPX2、Aurora-A表达诊断喉鳞状细胞癌的价值,并分析Twist2、Snail、TPX2、Aurora-A表达与喉鳞状细胞癌临床病理特征的关系。结果:喉鳞状细胞癌组织中Twist2阳性表达、Snail阳性表达、TPX2阳性表达、Aurora-A阳性均高于癌旁组织(P<0.05)。ROC曲线分析显示,Twist2、Snail、TPX2、Aurora-A表达诊断喉鳞状细胞癌的AUC分别为0.674、0.697、0.818、0.780,均具有较高准确性;诊断敏感度、特异度:Twist2为54.5%、80.3%;Snail为56.1%、83.3%;TPX2为69.7%、93.9%;Aurora-A为63.6%、92.4%,诊断具有一定价值。喉鳞状细胞癌组织中Twist2阳性表达、Snail阳性表达、TPX2阳性表达、Aurora-A阳性均与年龄、性别、临床分型无相关(P>0.05),但喉鳞状细胞癌组织中Twist2阳性表达、Snail阳性表达、TPX2阳性表达、Aurora-A阳性均与甲状软骨累及、TNM分期、分化程度、淋巴结转移、远处转移存在相关(P<0.05)。结论:Twist2、Snail、TPX2、Aurora-A在喉鳞状细胞癌组织中高表达,与患者临床病理特征相关密切,可作为临床诊断喉鳞状细胞癌有效指标。

子宫内膜癌组织CTGF、TWIST1、NCAPH的表达与临床病理特征及预后的关系

目的 探讨子宫内膜癌组织中结缔组织生长因子(CTGF)、TWIST1和非平滑肌细胞凝聚素Ⅰ复合亚单位H(NCAPH)蛋白的表达,以及与肿瘤临床病理特征及患者预后的关系。方法 回顾性分析2017年1月至2019年1月于南通大学附属海安医院行全子宫切除术140例患者的临床资料,其中术后病理证实为子宫内膜癌68例(子宫内膜癌组)、不典型增生42例(不典型增生组)和正常子宫内膜30例(正常组)。采用免疫组化染色法检测3组CTGF、TWIST1和NCAPH蛋白的阳性表达率,分析其与子宫内膜癌患者的肿瘤病理特征和预后的关系。结果 子宫内膜癌组CTGF、TWIST1和NCAPH蛋白的阳性表达率均明显高于不典型增生组和正常组,差异有统计学意义(P<0.05),而不典型增生组和正常组上述指标比较差异无统计学意义(P>0.05)。子宫内膜癌组CTGF、TWIST1和NCAPH蛋白的阳性表达率与患者年龄、肿瘤分化程度和病理类型无相关性(P>0.05),而与肿瘤临床分期、淋巴结转移和肿瘤肌层浸润深度密切相关(P<0.05)。Kaplan-Meier生存分析显示,子宫内膜癌组CTGF、TWIST1和NCAPH阳性表达患者的累积总生存率均明显低于阴性表达患者(P<0.05)。单因素和多因素COX回归分析显示,国际妇产科联盟(FIGO)分期Ⅲ~Ⅳ期、淋巴结转移阳性、CTGF、TWIST1和NCAPH阳性表达是患者死亡的独立危险因素(P<0.05)。结论 子宫内膜癌患者组织CTGF、TWIST1和NCAPH表达上调可能与肿瘤发生和发展密切相关,三者可作为预测生存预后的重要生物标志物。

大长径比远程制导火箭弹自适应Super-twisting控制方法

针对具有轻质薄壳结构的大长径比远程制导火箭弹刚体-弹性体耦合动力学控制难题,提出了一种自适应Super-twisting控制方法。首先,将大长径比远程制导火箭弹的弹性模态与外部干扰项视为归一化扰动,建立了考虑参数、模型不确定性的刚体-弹性体耦合动力学模型。针对外部扰动难以实时精准获取的问题,设计了有限时间收敛干扰观测器,能够在大范围气动参数摄动情形下对远程制导火箭弹外部扰动进行实时精准估计。为提高系统鲁棒性,设计了基于Super-twisting算法的自适应有限时间控制方法,使系统能根据状态误差自动调节控制参数,在削弱高频抖振的同时有效提高抗干扰能力。仿真结果表明:所提控制方法在弹体±15%气动参数摄动的条件下,由平飞状态转变为跟踪幅值为10的正弦弹道倾角指令时,实现3.2 s内弹道倾角跟踪误差收敛至0.002°,可实现远程制导火箭弹姿态平稳跟踪控制弹道倾角指令。