Bamboo shoots grow quickly through the rapid elongation of intemodes. In order to veri~ whether DNA methylation affects bamboo shoot elongation, we compared DNA methylation levels at five develop- mental stages of bam...Bamboo shoots grow quickly through the rapid elongation of intemodes. In order to veri~ whether DNA methylation affects bamboo shoot elongation, we compared DNA methylation levels at five develop- mental stages of bamboo （Phyllostachys praecox）, including young bamboo shoot of 40-cm at length （S1）, bamboo shoot of 2-cm at length （S2）, young leaf （Y1）, leaf （Y2）, and aging leaf （M）, by using amplified fragment length polymorphism （AFLP） based on KpnI/MseI and Acc65I/MseI platforms. The polymorphism ratio of methylated to un- methylated DNA sites in bamboo shoot （$1 and $2） was higher than that in leaf （Y1, Y2 and M）. The polymorphism ratio of methylated to un- methylated DNA sites in $2 was highest （32%）. Our results suggest that DNA methylation changed greatly at bamboo growing stages, especially in stage of rapid elongation of intemodes.展开更多
The test site was located in Jincheng Town, Lin’an City, Zhejiang Province. The leaves, culms and rhizomes of two-year-old and three-year-old flowering individuals or nonflowering individuals were taken from the same...The test site was located in Jincheng Town, Lin’an City, Zhejiang Province. The leaves, culms and rhizomes of two-year-old and three-year-old flowering individuals or nonflowering individuals were taken from the same bamboo （Phyllostachys praecox f. prevernalis） forest. The culms and rhizomes of one-year-old flowering or nonflowering were also taken from the same bamboo forest. Based on analysis of the content of endogenous hormones, aminoacid and nutrition in flowering stage of bamboo, the results showed that abscisic acid（ABA） had main effect on accelerating its flowering and the increase of cytokinin（CTK） was also one reason for bringing flower, but indole3acetic acid（IAA）antagonized on its flowering. Total amino-acid in the nonflowering bamboo was 23.01% higher than that in flowering bamboo. Aspartic acid （ASP） could delay flowering. Protein, especially protein in leaves had main effect on senescence procedure. Increase of phosphorus content in flowering bamboo could accelerate metabolizing, and therefore promote senescence of bamboo.展开更多
基金funded by the Natural Science Foundation of China (30800879)
文摘Bamboo shoots grow quickly through the rapid elongation of intemodes. In order to veri~ whether DNA methylation affects bamboo shoot elongation, we compared DNA methylation levels at five develop- mental stages of bamboo （Phyllostachys praecox）, including young bamboo shoot of 40-cm at length （S1）, bamboo shoot of 2-cm at length （S2）, young leaf （Y1）, leaf （Y2）, and aging leaf （M）, by using amplified fragment length polymorphism （AFLP） based on KpnI/MseI and Acc65I/MseI platforms. The polymorphism ratio of methylated to un- methylated DNA sites in bamboo shoot （$1 and $2） was higher than that in leaf （Y1, Y2 and M）. The polymorphism ratio of methylated to un- methylated DNA sites in $2 was highest （32%）. Our results suggest that DNA methylation changed greatly at bamboo growing stages, especially in stage of rapid elongation of intemodes.
文摘The test site was located in Jincheng Town, Lin’an City, Zhejiang Province. The leaves, culms and rhizomes of two-year-old and three-year-old flowering individuals or nonflowering individuals were taken from the same bamboo （Phyllostachys praecox f. prevernalis） forest. The culms and rhizomes of one-year-old flowering or nonflowering were also taken from the same bamboo forest. Based on analysis of the content of endogenous hormones, aminoacid and nutrition in flowering stage of bamboo, the results showed that abscisic acid（ABA） had main effect on accelerating its flowering and the increase of cytokinin（CTK） was also one reason for bringing flower, but indole3acetic acid（IAA）antagonized on its flowering. Total amino-acid in the nonflowering bamboo was 23.01% higher than that in flowering bamboo. Aspartic acid （ASP） could delay flowering. Protein, especially protein in leaves had main effect on senescence procedure. Increase of phosphorus content in flowering bamboo could accelerate metabolizing, and therefore promote senescence of bamboo.
