关于Barcode自助借还机在高校图书馆应用满意度的实证研究——以江苏海洋大学图书馆为例

随着智慧图书馆概念的提出,Barcode(条形码)识别模式自助借还机作为一项新型的、日趋成熟的服务方式正广泛应用于图书馆。基于此,以Barcode自助借还机的使用情况为调研对象,了解读者和管理员使用后的满意度,以便发现自助借还机在使用过程中的各种利弊,并提出解决问题的有效途径。

COI序列:影响动物分类学与生态学的DNA barcode

DNA barcode是一段特殊的、可用于物种鉴定的DNA序列。目前在动物中最常用的DNA barcode是细胞色素C氧化酶1号基因(COI)的部分序列。随着标准数据库的建立,基于COI基因的DNA barcode在动物分类学和生态学中得到了广泛应用。但是,采用COI基因作为DNA barcode所隐含的涉及线粒体的进化历史、遗传特性和物种成种时间的默认前提,并非完全成立,由此引发了许多问题。本文阐述了基于COI基因的DNA barcode对分类学和生态学的影响,目前存在的问题,以及可能的研究方向。

Barcode技术在ERP框架下生产过程管理中的应用研究

针对离散生产企业车间管理的黑箱作业,提出Barcode技术在企业资源计划(ERP)下生产过程管理中的应用框架,总结出Barcode技术在ERP下生产过程管理应用架构的三个主要技术,并介绍一种先进物料清单(BOM)设计和条码组合设计的思路。最后结合实施的多个案例分析此模式在离散制造业的应用。

Barcode技术:增加输血安全性的作用

背景 错误的血液成分输入是最常见的输血相关的严重事故,这些频繁发生的错误原因包括病人的错误辨识。设计和方法 本研究评估Barcode病人辨识系统,包括手提电脑对采集血样的兼容性试验和血液管理的作用,和应用Barcode前后的作用。结果 基线检查显示尤其是病人辨识的实用性差。

基于barcode4j的独立学院图书馆资产管理平台——以华南师范大学增城学院为例

文章阐述了Barcode4j的原理和开发方法,对平台的设计与开发进行了分析,提出了在校园网上开发图书馆资产网络管理平台的思路。

Barcode与RFID识别模式的自助借还系统比较分析

在论述Barcode与RFID识别模式图书馆自助借还系统原理的基础上,从成本、使用、管理和安全4方面分析两个模式现阶段应用于图书馆自助系统的优劣,并对上述两种识别模式的未来发展趋势进行了分析与展望。

Application of Evolutionary Encryption 2D Barcode Generation Technology in Agricultural Product Quality and Safety Traceability System

Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective.

A WATERMARKING ALGORITHM BASED ON PERMUTATION AND 2-D BARCODE

This letter presents a method for digital image watermarking for copyright protection. This technique produces a watermarked image that closely retains the quality of the original host image while concurrently surviving various image processing operations such as lowpass/highpass filtering, lossy JPEG compression, and cropping. This image watermarking algorithm takes full advantage of permutation and 2-D barcode (PDF417). The actual watermark embedding in spatial domain is followed using permutated image for improving the resistance to image cropping. Much higher watermark robustness is obtainable via a simple forward error correction technique, which is the main feature of PDF417 codes. Additional features of this technique include the easy determination of the existence of the watermark and that the watermark verification procedure does not need the original host image. The experimental results demonstrate its effectiveness.

DNA Barcode Screening and Preliminary Study on Phylogeny of Sea Snake Based on Genes COI and cytb

[Objectives]The most common gene fragment used in animal DNA barcode technology is COI,but it is not necessarily suitable for all species.This study was conducted to screen genes suitable for the DNA barcode of sea snakes.[Methods]All COI and cytb gene sequences on GenBank were searched and downloaded.After the comparison with Mega software,clustering trees of MrBayes system were established.[Results]Interspecies distances were greater than intraspecies distances for the two genes.The topological structures of their molecular hierarchical clustering trees were clear,and the support rates were high.[Conclusions]Therefore,it is concluded that not the DNA barcode of each species must be gene COI.Cytb is more suitable in terms of the mitochondrial gene of sea snakes.

DNA Barcodes in Fig Cultivars (Ficus carica L.) Using ITS Regions of Ribosomal DNA, the psbA-trnH Spacer and the matK Coding Sequence

Molecular markers provide a useful method for genotype characterization and allow a high precision determination of the genetic relationship between cultivars and varieties. A system based on DNA sequences—which is known as DNA barcoding—will choose one or several standard loci which can be sequenced and compared to differentiate between species. In this research, the ITS, matK, and trnH-psbA sequences were evaluated for the molecular identification of seven F. carica genotypes, generating complete sequences for the first two loci, but unable to produce bidirectional sequences by using the trnH-psbA sequence. The ITS sequence presented the highest variation rates, while the phylogeny constructed with the matK sequence obtained the highest percentage of solved monophyletic groups. Through Pearson’s correlation analysis, it was possible to determine the existence of a significant correlation between the ITS region and psbA-trnH, and the matK and psbA-trnH sequences, but not between ITS and matK. The phylogenies constructed with the ITS + matK barcodes and ITS + matK + psbA-trnH presented the highest percentage for resolution. However, considering the cost efficiency and the facilitated recovery by using PCR, the matK + ITS combination is recommended.

Emergence of Plastidial Intergenic Spacers as Suitable DNA Barcodes for Arid Medicinal Plant <i>Rhazya stricta</i>

The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this species. The authenticity of R. stricta and other medicinal plants and herbs procured from local markets can be questionable due to a lack of clear phenotypic traits. DNA barcoding is an emerging technology for rapid and accurate species identification. In this study, six candidate chloroplastid barcodes were investigated for the authentication of R. stricta. We compared the DNA sequences from fifty locally collected and five market samples of R. stricta with database sequences of R. stricta and seven closely related species. We found that the coding regions matK, rbcL, rpoB, and rpoC1 were highly similar among the taxa. By contrast, the intergenic spacers psbK-psbI and atpF-atpH were variable loci distinct for the medicinal plant R. stricta. psbK-psbI clearly discriminated R. stricta samples as an efficient single locus marker, whereas a two-locus marker combination comprising psbK-psbI + atpF-atpH was also promising according to results from the Basic Local Alignment Search Tool and a maximum likelihood gene tree generated using PHyML. Two-dimensional DNA barcodes (i.e., QR codes) for the psbK-psbI and psbK-psbI + atpF-atpH regions were created for the validation of fresh or dried R. stricta samples.

Earthquake Barcode from a Single-Degree-of-Freedom System

Earthquake is a violent and irregular ground motion that can severely damage structures. In this paper we subject a single-degree-of-freedom system, consisting of spring and damper, to an earthquake excitation, and meanwhile investigate the response behavior from a novel theory about the dynamical system, by viewing the time-varying signum function of It can reflect the characteristic property of each earthquake through and the second component of f, where is a time-sampling record of the acceleration of a ground motion. The barcode is formed by plotting with respect to time. We analyze the complex jumping behavior in a barcode and an essential property of a high percentage occupation of the first set of dis-connectivity in the barcode from four strong earthquake records: 1940 El Centro earthquake, 1989 Loma earthquake, and two records of 1999 Chi-Chi earthquake. Through the comparisons of four earthquakes, we can observe that strong earthquake leads to large percentage of the first set of dis-connectivity.

A High-Density Ternary Barcode Detection System with a Dual-Bias Differential Method

A dual-bias differential method is presented for increasing the detection range of a ternary barcode detection system. The system is provided with a second differential delay circuit with bias control to process optimally gray signals by lowering their averaged level using a clamping circuit. This is added to the primary conventional differential delay circuit without bias control and a comparator to process optimally black signals based on the envelope-differential fixed-period delay (EDFPD) detection technique. This method enables the system to detect over a longer range at high speeds while being capable of handling a large amount of information. The estimate results of gray and white code widths against the clamp bias made through the dynamic operation simulation of a differential circuit using SPICE were nearly consistent with the experimental results. Thereby we can conclude that the dynamic simulation is effective for estimation of an optimum clamp bias voltage. It was confirmed that the detection range of the system with a clamp bias voltage of ?0.4 V for a minimum bar width W = 0.25 mm was 1.4 times that of the conventional EDFPD detection technique. In addition, the system operated at a maximum scanning speed of 7.7 times that of conventional CCD cameras under the practical detection range. The system with clamp bias control is expected to enable the real-time identification of goods on production lines and in automated warehouses.

Identification of Lablab Semen Album by DNA Barcode Technology

[Objectives] To identify ITS2 barcode of Lablab Semen Album and its adulterants,and provide a new method for the identification of Lablab Semen Album. [Methods] The ITS2 sequence was amplified by PCR and sequenced bi-directionally. After splicing by Codon Code Aligner,the data were processed with the aid MEGA software to construct the cluster dendrogram( neighbor-joining,NJ tree). [Results]The ITS2 sequence of Lablab Semen Album had length of 218 bp; the constructed cluster dendrogram indicated that all species were monophyletic and could be distinguished from other species. [Conclusions] The ITS2 barcode can be used for rapid identification of Lablab Semen Album and its adulterants and this experiment further verified that DNA barcode technology is effective in identification of traditional Chinese medicines.

Barcode4J

简介：Barcode4J是一个用Java编写的条形码生成器。通过Apache License v2.0发布，因此它很容易应用于商业环境中。可以生成所有常见的1-D和2-D的条形码，其中包括DataMatrix和PDF417，并且能以多种形式输出，比如SVG、EPS、bitmaps和Java2D——而且你可以很容易地添加自定义输出格式。同时，Barcode4J还为不同的应用程序提供了插件，其中最重要的就是Apache FOP（格式化对象处理器）。有些用户也将Barcode4J部署为一个Servlet。

应用Barcode管理系统优化ERP的设计与实现

利用Barcode管理系统,结合使用掌上电脑(personal Digital Assistant, PDA)进一步优化企业资源计划(ERP)系统,实现仓库内的入库管理、盘点、库位查询、出库管理、车间报工、成品装箱等环节利用条码技术实时把数据传输至ERP上,做到精准监管、无缝衔接,使企业实时掌握仓库动态、物料库存、生产状况等,促进ERP系统实施更加高效。

Chloroplast gene matK holds the barcodes for identification of Momordica(Cucurbitaceae) species from Indian subcontinent

DNA barcoding is a supplementary tool in plant systematics,extensively used to resolve the species level controversies.This paper details the identification of DNA barcodes for seven species of Momordica,using the chloroplast gene mat K.Since the species M.cymbalaria has been confused as a member of the genus Luffa,26 accessions of Momordica belonging to seven Indian species and two accessions of Luffa acutangula were included in this study.Analysis of mat K sequences has yielded distinct barcodes in M.charantia var.charantia,M.subangulata subsp.renigera,M.cochinchinensis,M.balsamina,M.cymbalaria and also in Luffa acutangula.Evolutionary status of each species was reflected as nucleotide polymorphisms in each sequence.The wild species M.dioica and M.sahyadrica have yielded one barcode but failed to get differentiated.Further,this study provides conclusive proof that M.cymbalaria is a member of Momordica genus.The phylogram generated was successful to distinguish the monoecious species of this genus,M.charantia,M.balsamina and M.cymbalaria,from the dioecious species M.dioica,M.sahyadrica,M.subangulata subsp.renigera and M.cochinchinensis.Thus,mat K locus,by accumulating the evolutionary sequence variations,is proven efficient to differentiate the Momordica species and to reveal their relatedness.

Screening of DNA Barcode of Quarantine Phytophthora

Phytophtkora is genus of plant-damaging Oomycetes,whose member species are capable of causing enormous economic losses on crops worldwide.In the present study,four candidate genes ITS,CO1,EF-1α and β-tubulin were tested using 123 strains of 80 species of Phytophthora to investigate the feasibility of serving as DNA barcoding markers.The results showed that among the four candidate genes,ITS and CO1 had the highest success rate of PCR amplification and sequencing,up to 100%and 96.7%.There were obvious barcode gaps in ITS,CO1 and β-tubulin,but their frequency distributions of intra-and interspecific genetic distances were slightly overlapped.Wilcoxon rank sum test on intraspecific genetic distances of the four genes showed ITS = CO1 = β-tubulin = EF-1α,indicating they had the same effect on intraspecific discrimination,while the test on interspecific genetic distances of the four genes showed ITS > CO1 > β-tubulin > EF-1α.In summary,ITS and CO1 should be used in combination as the primary barcodes,β-tubulin as the complementary barcode for the identification of 11 quarantine Phytophthora species.

The Potential of DNA Barcode-Based Delineation Using Seven Putative Candidate Loci of the Plastid Region in Inferring Molecular Diversity of Cowpea at Sub-Species Level

The novelty and suitability of the mitochondrial gene CO1 in DNA barcoding as a reliable identification tool in animal species are undisputed. This is attributed to its standardized sequencing segment of the mitochondrial cytochrome c oxidase-1 gene (CO1) which has the necessary universality and variability making it a generally acceptable barcode region. CO1 is a haploid single locus that is uniparentally-inherited. Protein-coding regions are present in high-copy numbers making it an ideal barcode. The mitochondrial oxidase subunit I (COI) gene is a robust barcode with a suitable threshold for delineating animals and is not subject to drastic length variation, frequent mononucleotide repeats or microinversions. However, a low nucleotide substitution rate of plant mitochondrial genome [mtDNA] precludes the use of CO1 as a universal plant DNA barcode and makes the search for alternative barcode regions necessary. Currently, there exists no universal barcode for plants. The plastid region reveals leading candidate loci as appropriate DNA barcodes yet to be explored in biodiversity studies in Kenya. Four of these plastid regions are portions of coding genes (matK, rbcL, rpoB, and rpoC1), and three noncoding spacers (atpF-atpH, trnH-psbA, and psbK-psbL) which emerge as ideal candidate DNA loci. While different research groups propose various combinations of these loci, there exists no consensus;the lack thereof impedes progress in getting a suitable universal DNA barcode. Little research has attempted to investigate and document the applicability and extend of effectiveness of different DNA regions as barcodes to delineate cowpea at subspecies level. In this study we sought to test feasibility of the seven putative candidate DNA loci singly and in combination in order to establish a suitable single and multi-locus barcode regions that can have universal application in delineating diverse phylogeographic groups of closely related Kenyan cowpea variants. In this study, our focus was based on genetic parameters including analyses of intra- and infra-specific genetic divergence based on intra- and infra-specific K2P distances;calculation of Wilcoxon signed rank tests of intra-specific divergence among loci and coalescence analyses to delineate independent genetic clusters. Knowledge of DNA candidate loci that are informative will reveal the suitability of DNA barcoding as a tool in biodiversity studies. Results of this study indicate that: matK, trnH-psbA, psbK-psbL, and rbcL are good barcodes for delineating intra and infraspecific distances at single loci level. However, among the combinations, matK + trnH-psbA, rpoB + atpF-atpH + matK are the best barcodes in delineating cowpea subvariants. rbcL gene can be a suitable barcode marker at single locus level, but overall, multi locus approach appears more informative than single locus approach. The present study hopes to immensely contribute to the scanty body of knowledge on the novelty of DNA barcoding in cataloguing closely related cowpea variants at molecular level and hopes to open up future research on genomics and the possibility of use of conserved regions within DNA in inferring phylogenetic relationships among Kenyan cowpea variants.

RNA barcode segments for SARS-CoV-2 identification from HCoVs and SARSr-CoV-2 lineages

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the pathogen responsible for coronavirus disease 2019(COVID-19),continues to evolve,giving rise to more variants and global reinfections.Previous research has demonstrated that barcode segments can effectively and cost-efficiently identify specific species within closely related populations.In this study,we designed and tested RNA barcode segments based on genetic evolutionary relationships to facilitate the efficient and accurate identification of SARS-CoV-2 from extensive virus samples,including human coronaviruses(HCoVs)and SARSr-CoV-2 lineages.Nucleotide sequences sourced from NCBI and GISAID were meticulously selected and curated to construct training sets,encompassing 1733 complete genome sequences of HCoVs and SARSr-CoV-2 lineages.Through genetic-level species testing,we validated the accuracy and reliability of the barcode segments for identifying SARS-CoV-2.Subsequently,75 main and subordinate species-specific barcode segments for SARS-CoV-2,located in ORF1ab,S,E,ORF7a,and N coding sequences,were intercepted and screened based on single-nucleotide polymorphism sites and weighted scores.Post-testing,these segments exhibited high recall rates(nearly 100%),specificity(almost 30%at the nucleotide level),and precision(100%)performance on identification.They were eventually visualized using one and two-dimensional combined barcodes and deposited in an online database(http://virusbarcodedatabase.top/).The successful integration of barcoding technology in SARS-CoV-2 identification provides valuable insights for future studies involving complete genome sequence polymorphism analysis.Moreover,this cost-effective and efficient identification approach also provides valuable reference for future research endeavors related to virus surveillance.