Effect of electro-acupuncture on basic fibroblast growth factor protein and mRNA expression in hippocampal dentate gyrus of spleen deficiency rats

BACKGROUND： Spleen deficiency in traditional Chinese medicine refers to the functional disorder of spleen, pancreas, intestines, and nervous system in modern medicine. OBJECTIVE; To test whether electro-acupuncture could alter basic fibroblast growth factor （bFGF） protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats. DESIGN, TIME AND SETTING： The randomized, controlled, in vivo animal experiment was performed at the National LeveI-B Laboratory of Clinical Cell Molecule and Biology in Shenzhen Hospital of Traditional Chinese Medicine, between March and November in 2008. MATERIALS： Reserpine injection was produced by Guangdong Bangmin Pharmaceutical Co. Rhubarb extract granule preparation was produced by Guangdong Yifang Pharmaceutical. Huanqiu Brand sterile acupuncture pin was provided by Suzhou Acupuncture Supplies, China. Huatuo Brand electroacupuncture instrument （type SDZ-II） was purchased from Suzhou Medical Appliance Factory, China. METHODS： A total of 96 male Sprague Dawley rats were randomly assigned to control （n = 32） and induction （n = 64） groups. Spleen deficiency was induced via intraperitoneal injection of reserpine and intragastric administration of rhubarb. The successful models were randomized into two groups： model and electro-acupuncture, with 32 rats in each group. Electro-acupuncture was administered at Zusanfi （ST 36） and Sanyinjiao （SP 6） acupoints using a condensation wave and rarefaction （condensation wave 15 Hz） at a strength of 6-15 V for 20 minutes, once per day. The appearance of a slight shiver in the corresponding locus was taken as the standard. According to electro- acupuncture time points, each group was assigned to four subgroups at 7, 14, 28, and 49 days, respectively, with eight rats in each subgroup. Immunohistochemical staining, image analysis, and reverse-transcription polymerase chain reaction were performed at different time points. MAIN OUTCOME MEASURES： bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats. RESULTS： After 7 days of electro-acupuncture therapy, bFGF protein and mRNA expression significantly increased compared with the model and control groups （P 〈 0.05）. After 14 days, bFGF protein and mRNA expression decreased until 28 days, where levels were then equal to the model group and greater than the control group （P 〈 0.05）. After 49 days, the above indices remained increased in the electro-acupuncture group compared to the model and control groups （P 〈 0.05）. CONCLUSION： Continuous electro-acupuncture maintained a high level of bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.

桂林西瓜霜对宫颈柱状上皮外移患者碱性成纤维细胞生长因子mRNA的影响

目的探讨桂林西瓜霜对宫颈柱状上皮外移修复的分子生物学机制。方法 160例宫颈柱状上皮外移患者,按随机数字表法分为两组,观察组80例用桂林西瓜霜局部喷敷,对照组80例用碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)-胶原蛋白海绵局部贴敷。应用RT-PCR检测两组宫颈组织治疗前后bFGFmRNA表达。结果治疗前,观察组和对照组宫颈柱状上皮外移组织中bFGFmRNA表达分别为0.55±0.10、0.58±0.13,组间比较差异无统计学意义(P>0.05);治疗后两组表达均显著增加,分别为0.82±0.17、0.78±0.15,与本组治疗前比较差异均有统计学意义(P<0.01),组间比较差异无统计学意义(P>0.05)。结论桂林西瓜霜对宫颈柱状上皮外移上皮组织bFGFmRNA表达有增强作用。

骨软骨瘤和骨恶性肿瘤VEGF mRNA和bFGF mRNA表达及意义(英文)

目的 研究骨软骨瘤和骨恶性肿瘤VEGFmRNA ,bFGFmRNA表达及其临床病理意义。方法 骨肿瘤脱钙后常规石蜡包埋切片原位杂交染色法。结果 15例骨软骨瘤仅 1例VEGFmRNA ,bFGFmRNA共同阳性 ,4 5例骨恶性肿瘤VEGFmRNA阳性 2 5例 (5 5 .6 % )和bFGFmRNA阳性 2 8例 (6 2 .2 % ) ,骨恶性肿瘤两者阳性率明显高于骨软骨瘤 (P <0 .0 0 5 )。组织学分级I级和无原发灶外转移骨恶性肿瘤VEGFmRNA ,bFGFmRNA阳性率明显低于组织学分级Ⅲ级和原发灶外转移病例。骨恶性肿瘤中VEGFmRNA表达与bFGFmRNA表达呈密切正相关。结论 VEGFmRNA和bFGFmRNA表达可能与骨恶性肿瘤恶性程度、生物学行为和转移发生有密切关系 ,两者均为重要促血管生成因子。

中风康对局灶性脑缺血大鼠脑组织bFGFmRNA表达的影响

目的探讨中风康对局灶性脑缺血大鼠脑组织碱性成纤维细胞生长因子(bFGF)mRNA表达的影响。方法采用线栓法复制局灶性脑缺血大鼠模型,采用RT-PCR技术于脑缺血6,12,24,48h动态观察局灶性脑缺血大鼠脑组织bFGFmRNA表达的变化,并在缺血24h以步长脑心通为对照,观察中风康对bFGFmRNA表达的影响。结果与假手术组比较,局灶性脑缺血6h,模型组脑组织bFGFmRNA表达量开始增加,至24h达到高峰(P<0.01);与模型组比较,脑缺血24h各治疗组脑组织bFGFmRNA表达量均有不同程度升高(P<0.05或P<0.01),以高剂量组升高最为显著(P<0.01)。结论中风康可在转录水平提高脑梗死大鼠脑组织bFGFmRNA表达,有效减轻局灶性脑梗死引起的缺血性损伤。

碱性成纤维细胞生长因子对脑出血大鼠神经细胞凋亡和Bax、Bcl-2基因表达的影响

目的观察碱性成纤维细胞生长因子(bFGF)对大鼠脑出血后神经细胞凋亡及血肿周围大脑皮质和出血侧海马Bax、Bcl-2基因表达的影响。方法采用脑内注射胶原酶建立大鼠脑出血模型,应用TUNEL法测定大鼠大脑皮质、海马神经细胞凋亡数目。及应用采用半定量逆转录酶聚合链反应(RT-PCR),检测脑出血后血肿周围大脑皮质和出血侧海马的Bax mRNA、Bcl-2 mRNA表达。结果(1)bFGF组的皮质和海马神经细胞凋亡数比生理盐水(NS)对照组明显减少(P<0.05)。(2)bFGF组的血肿周围大脑皮质和出血侧海马Bax mRNA表达比NS对照组明显减少(P<0.05);而Bcl-2 mRNA表达比NS对照组明显增高(P<0.05)。结论bFGF提高大鼠脑出血后大脑组织和海马Bcl-2 mRNA的表达,降低Bax mRNA的表达,抑制脑出血后神经细胞凋亡。