Laser welding (LW) becomes one of the most economical high quality joining processes. LW offers the advantage of very controlled heat input resulting in low distortion and the ability to weld heat sensitive components...Laser welding (LW) becomes one of the most economical high quality joining processes. LW offers the advantage of very controlled heat input resulting in low distortion and the ability to weld heat sensitive components. To exploit efficiently the benefits presented by LW, it is necessary to develop an integrated approach to identify and control the welding process variables in order to produce the desired weld characteristics without being forced to use the traditional and fastidious trial and error procedures. The paper presents a study of weld bead geometry characteristics prediction for laser overlap welding of low carbon galvanized steel using 3D numerical modelling and experimental validation. The temperature dependent material properties, metallurgical transformations and enthalpy method constitute the foundation of the proposed modelling approach. An adaptive 3D heat source is adopted to simulate both keyhole and conduction mode of the LW process. The simulations are performed using 3D finite element model on commercial software. The model is used to estimate the weld bead geometry characteristics for various LW parameters, such as laser power, welding speed and laser beam diameter. The calibration and validation of the 3D numerical model are based on experimental data achieved using a 3 kW Nd:Yag laser system, a structured experimental design and confirmed statistical analysis tools. The results reveal that the modelling approach can provide not only a consistent and accurate prediction of the weld characteristics under variable welding parameters and conditions but also a comprehensive and quantitative analysis of process parameters effects on the weld quality. The results show great concordance between predicted and measured values for weld bead geometry characteristics, such as depth of penetration, bead width at the top surface and bead width at the interface between sheets, with an average accuracy greater than 95%.展开更多
AIM:To explore the method for early diagnosis of gastric cancer by screening the expression spectrum of saliva protein in gastric cancer patients using mass spectrometry for proteomics.METHODS:Proportional peptide mas...AIM:To explore the method for early diagnosis of gastric cancer by screening the expression spectrum of saliva protein in gastric cancer patients using mass spectrometry for proteomics.METHODS:Proportional peptide mass fingerprints were obtained by analysis based on proteomics matrix-assisted laser desorption ionization time-of-flight/mass spectrometry.A diagnosis model was established using weak cation exchange magnetic beads to test saliva specimens from gastric cancer patients and healthy subjects.RESULTS:Significant differences were observed in the mass to charge ratio(m/z) peaks of four proteins(1472.78 Da,2936.49 Da,6556.81 Da and 7081.17 Da) between gastric cancer patients and healthy subjects.CONCLUSION:The finger print mass spectrum of saliva protein in patients with gastric cancer can be established using gastric cancer proteomics.A diagnostic model for distinguishing protein expression mass spectra of gastric cancer from non-gastric-cancer saliva can be established according to the different expression of proteins 1472.78 Da,2936.49 Da,6556.81 Da and 7081.17 Da.The method for early diagnosis of gastric cancer is of certain value for screening special biological markers.展开更多
In order to maintain the structural consistency during the welding of precipitation hardened copperchromium-zirconium(PH-CuCrZr)alloy components,electron beam welding(EBW)process was employed.Experimental study and nu...In order to maintain the structural consistency during the welding of precipitation hardened copperchromium-zirconium(PH-CuCrZr)alloy components,electron beam welding(EBW)process was employed.Experimental study and numerical modeling of EBW process during welding of PH-CuCrZr alloy components were carried out.A 3D finite element model was developed to predict the output responses(bead penetration and bead width)as a function of EBW input parameters(beam current,acceleration voltage and weld speed).A combined circular and conical source with Gaussian heat distribution was used to model the deep penetration characteristic of the EBW process.Numerical modeling was carried out by developing user defined function in Ansys software.Numerical predictions were compared with the experimental results which had a good agreement with each other.The developed model can be used for parametric study in wide range of problems involving complex geometries which are to be welded using EBW process.The present work illustrates that the input current with a contribution of 44.56%and 81.13%is the most significant input parameter for the bead penetration and bead width,respectively.展开更多
Objective To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed...Objective To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed proteins in the urine of renal clear cell carcinoma (RCCC) and its value in the early diagnosis of RCCC.Methods Eleven newly diagnosed patients (10 males and 1 female, aged 46-78, mean 63 years) of renal clear cell carcinoma by biopsy and 10 healthy volunteers (all males, aged 25-32, mean 29.7 years) were enrolled in this study. Urine samples of the RCCC patients and healthy controls were collected in the morning.Weak cation exchange (WCX) bead-based MALDI-TOF MS technique was applied in detecting differential protein peaks in the urine of RCCC. ClinProTools2.2 software was utilized to determine the characteristic proteins in the urine of RCCC patients for the predictive model of RCCC.Results The technique identified 160 protein peaks in the urine that were different between RCCC patients and health controls; and among them, there was one peak (molecular weight of 2221.71 Da) with statistical significance (P=0.0304). With genetic algorithms and the support vector machine, we screened out 13 characteristic protein peaks for the predictive model.Conclusions The application of WCX magnetic bead-based MALDI-TOF MS in detecting differentiallyexpressed proteins in urine may have potential value for the early diagnosis of RCCC.展开更多
Duplex stainless steel was formed through welding wire and arc additive manufacturing(WAAM)using tungsten inert gas.The effects of wire feeding speed(WFS),welding speed(WS),welding current,and their interaction on the...Duplex stainless steel was formed through welding wire and arc additive manufacturing(WAAM)using tungsten inert gas.The effects of wire feeding speed(WFS),welding speed(WS),welding current,and their interaction on the weld bead width and height were discussed.Back-propagation(BP)neural network algorithm prediction model was established by taking the bead width and height as the output layer,and the network weight and threshold values were optimized using the particle swarm optimization(PSO)algorithm to obtain the prediction model of bead width and height.The predicted results were verified by experiments.Results show that the weld bead width increases with the increase in WFS and the welding current and decreases with WS.The smaller the WFS,the faster the WS,which is beneficial for the generation of equiaxed crystals.The smaller the welding current,the faster the cooling speed of the metal melt,which is conducive to the formation of dendrites.The interaction among WS,wire feed speed,and welding current has a significant effect on the bead width.The weld bead height is positively correlated with the wire feed speed and negatively correlated with the WS and current.The interaction between the wire feed speed and WS is significant.The optimized WAAM process parameters for duplex stainless steel are a wire feed speed of 200 cm/min,WS of 24 cm/min,and welding current of 160 A.The maximum error of the BP neural network in predicting the weld bead width and height is 7.74%,and the maximum error between the predicted and experimental values of the BP-PSO neural network is 4.27%.This finding indicates that the convergence speed is fast,improving the prediction accuracy.展开更多
文摘Laser welding (LW) becomes one of the most economical high quality joining processes. LW offers the advantage of very controlled heat input resulting in low distortion and the ability to weld heat sensitive components. To exploit efficiently the benefits presented by LW, it is necessary to develop an integrated approach to identify and control the welding process variables in order to produce the desired weld characteristics without being forced to use the traditional and fastidious trial and error procedures. The paper presents a study of weld bead geometry characteristics prediction for laser overlap welding of low carbon galvanized steel using 3D numerical modelling and experimental validation. The temperature dependent material properties, metallurgical transformations and enthalpy method constitute the foundation of the proposed modelling approach. An adaptive 3D heat source is adopted to simulate both keyhole and conduction mode of the LW process. The simulations are performed using 3D finite element model on commercial software. The model is used to estimate the weld bead geometry characteristics for various LW parameters, such as laser power, welding speed and laser beam diameter. The calibration and validation of the 3D numerical model are based on experimental data achieved using a 3 kW Nd:Yag laser system, a structured experimental design and confirmed statistical analysis tools. The results reveal that the modelling approach can provide not only a consistent and accurate prediction of the weld characteristics under variable welding parameters and conditions but also a comprehensive and quantitative analysis of process parameters effects on the weld quality. The results show great concordance between predicted and measured values for weld bead geometry characteristics, such as depth of penetration, bead width at the top surface and bead width at the interface between sheets, with an average accuracy greater than 95%.
基金Supported by The National Natural Science Foundation of China,No. 30640071
文摘AIM:To explore the method for early diagnosis of gastric cancer by screening the expression spectrum of saliva protein in gastric cancer patients using mass spectrometry for proteomics.METHODS:Proportional peptide mass fingerprints were obtained by analysis based on proteomics matrix-assisted laser desorption ionization time-of-flight/mass spectrometry.A diagnosis model was established using weak cation exchange magnetic beads to test saliva specimens from gastric cancer patients and healthy subjects.RESULTS:Significant differences were observed in the mass to charge ratio(m/z) peaks of four proteins(1472.78 Da,2936.49 Da,6556.81 Da and 7081.17 Da) between gastric cancer patients and healthy subjects.CONCLUSION:The finger print mass spectrum of saliva protein in patients with gastric cancer can be established using gastric cancer proteomics.A diagnostic model for distinguishing protein expression mass spectra of gastric cancer from non-gastric-cancer saliva can be established according to the different expression of proteins 1472.78 Da,2936.49 Da,6556.81 Da and 7081.17 Da.The method for early diagnosis of gastric cancer is of certain value for screening special biological markers.
文摘In order to maintain the structural consistency during the welding of precipitation hardened copperchromium-zirconium(PH-CuCrZr)alloy components,electron beam welding(EBW)process was employed.Experimental study and numerical modeling of EBW process during welding of PH-CuCrZr alloy components were carried out.A 3D finite element model was developed to predict the output responses(bead penetration and bead width)as a function of EBW input parameters(beam current,acceleration voltage and weld speed).A combined circular and conical source with Gaussian heat distribution was used to model the deep penetration characteristic of the EBW process.Numerical modeling was carried out by developing user defined function in Ansys software.Numerical predictions were compared with the experimental results which had a good agreement with each other.The developed model can be used for parametric study in wide range of problems involving complex geometries which are to be welded using EBW process.The present work illustrates that the input current with a contribution of 44.56%and 81.13%is the most significant input parameter for the bead penetration and bead width,respectively.
文摘Objective To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed proteins in the urine of renal clear cell carcinoma (RCCC) and its value in the early diagnosis of RCCC.Methods Eleven newly diagnosed patients (10 males and 1 female, aged 46-78, mean 63 years) of renal clear cell carcinoma by biopsy and 10 healthy volunteers (all males, aged 25-32, mean 29.7 years) were enrolled in this study. Urine samples of the RCCC patients and healthy controls were collected in the morning.Weak cation exchange (WCX) bead-based MALDI-TOF MS technique was applied in detecting differential protein peaks in the urine of RCCC. ClinProTools2.2 software was utilized to determine the characteristic proteins in the urine of RCCC patients for the predictive model of RCCC.Results The technique identified 160 protein peaks in the urine that were different between RCCC patients and health controls; and among them, there was one peak (molecular weight of 2221.71 Da) with statistical significance (P=0.0304). With genetic algorithms and the support vector machine, we screened out 13 characteristic protein peaks for the predictive model.Conclusions The application of WCX magnetic bead-based MALDI-TOF MS in detecting differentiallyexpressed proteins in urine may have potential value for the early diagnosis of RCCC.
基金Supported by Fujian Natural Science Foundation of China(Grant No.2020J05115)Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China(Grant No.2021ZZ123)+1 种基金Fuzhou University Testing Fund of precious apparatus(Grant No.2023T019)Quanzhou Science and Technology Plan Project of China(Grant No.2020C043R).
文摘Duplex stainless steel was formed through welding wire and arc additive manufacturing(WAAM)using tungsten inert gas.The effects of wire feeding speed(WFS),welding speed(WS),welding current,and their interaction on the weld bead width and height were discussed.Back-propagation(BP)neural network algorithm prediction model was established by taking the bead width and height as the output layer,and the network weight and threshold values were optimized using the particle swarm optimization(PSO)algorithm to obtain the prediction model of bead width and height.The predicted results were verified by experiments.Results show that the weld bead width increases with the increase in WFS and the welding current and decreases with WS.The smaller the WFS,the faster the WS,which is beneficial for the generation of equiaxed crystals.The smaller the welding current,the faster the cooling speed of the metal melt,which is conducive to the formation of dendrites.The interaction among WS,wire feed speed,and welding current has a significant effect on the bead width.The weld bead height is positively correlated with the wire feed speed and negatively correlated with the WS and current.The interaction between the wire feed speed and WS is significant.The optimized WAAM process parameters for duplex stainless steel are a wire feed speed of 200 cm/min,WS of 24 cm/min,and welding current of 160 A.The maximum error of the BP neural network in predicting the weld bead width and height is 7.74%,and the maximum error between the predicted and experimental values of the BP-PSO neural network is 4.27%.This finding indicates that the convergence speed is fast,improving the prediction accuracy.