Bone marrow-derived mesenchymal stem cell-derived exosomeloaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.

部分充填式窄幅钢箱-UHPC组合梁抗剪试验研究

目的为研究部分充填式窄幅钢箱-UHPC组合梁的抗剪性能,方法对4根倒置组合梁试件通过三点加载方式进行了静载试验,通过研究其负弯矩作用下的破坏形态及变形特征,得到了组合梁的荷载-挠度曲线、应变分布、荷载-滑移曲线、最终破坏形态等。结果试验研究结果表明:UHPC翼板显著提升了组合梁的变形能力和极限承载力;相较于试件SUCB-3(NC翼板),试件SUCB-1(1/2UHPC翼板和SUCB-4(全UHPC翼板))的承载力分别提高了18.2%和27.3%,变形能力则分别增强了38.02%和41.2%;钢纤维的添加能有效地抑制翼板裂缝的开展,钢纤维掺量从0提高到2%,试件的最大裂缝宽度从1.06 mm降低到0.92 mm,变形能力增强了23.61%,承载力则提高了4%。结论提出了考虑钢箱、UHPC翼板和充填混凝土贡献的组合梁抗剪承载力计算公式。

基于HMFDE和t-SNE的旋转机械故障诊断方法

针对旋转机械的故障特征提取较难,以及单一类型的特征无法全面反映故障特性的问题,提出了一种基于混合多尺度波动散布熵(HMFDE)、t分布-随机邻域嵌入(t-SNE)和郊狼优化算法(COA)优化极限学习机(ELM)的旋转机械故障诊断方法。首先,采用特征加权提出了混合多尺度波动散布熵方法,并将其用于提取旋转机械振动信号的故障特征;随后,采用t-SNE方法对混合故障特征进行了特征降维,挑选出了最能够反映故障特性的特征子集,构建了敏感特征样本;最后,采用郊狼优化算法对极限学习机的输入权重和隐含层阈值进行了优化,完成了旋转机械的故障识别和分类;以齿轮箱和滚动轴承故障数据集为对象,对基于HMFDE、t-SNE和COA-ELM的故障诊断方法进行了实验,验证了方法的有效性。研究结果表明:采用HMFDE-t-SNE-CAO-ELM故障诊断方法可以取得100%的故障识别准确率,该方法能够有效地诊断旋转机械的不同故障类型和损伤;相较于基于单一类型特征的故障诊断方法,其准确率分别可以提高0.68%、22.42%、29.18%(齿轮箱)和1.43%、8.23%、23.67%(滚动轴承),虽然牺牲了一定的计算效率,但准确率得到了明显的提高;相较于其他常规故障分类器,COA-ELM的故障识别准确率具有明显的优势。

Analysis of the Temperature Characteristics of High-speed Train Bearings Based on a Dynamics Model and Thermal Network Method

High-speed trains often use temperature sensors to monitor the motion state of bearings.However,the temperature of bearings can be affected by factors such as weather and faults.Therefore,it is necessary to analyze in detail the relationship between the bearing temperature and influencing factors.In this study,a dynamics model of the axle box bearing of high-speed trains is established.The model can obtain the contact force between the rollers and raceway and its change law when the bearing contains outer-ring,inner-ring,and rolling-element faults.Based on the model,a thermal network method is introduced to study the temperature field distribution of the axle box bearings of high-speed trains.In this model,the heat generation,conduction,and dispersion of the isothermal nodes can be solved.The results show that the temperature of the contact point between the outer-ring raceway and rolling-elements is the highest.The relationships between the node temperature and the speed,fault type,and fault size are analyzed,finding that the higher the speed,the higher the node temperature.Under different fault types,the node temperature first increases and then decreases as the fault size increases.The effectiveness of the model is demonstrated using the actual temperature data of a high-speed train.This study proposes a thermal network model that can predict the temperature of each component of the bearings on a high-speed train under various speed and fault conditions.

农用柴油机钢活塞销孔-销的摩擦副润滑特性分析

为解决钢活塞销孔-销摩擦副因同种材料摩擦配副问题以及钢材密度大和导热性能差所带来的润滑特性差的问题,该研究以D25TCIF农用柴油机钢活塞为对象,建立钢活塞连杆组传热模型和热弹性流体动力学模型,并开展钢活塞温度场测试试验与仿真验证。结合单因素扫值法和Box-Behnken多因素优化算法分析了销孔轴承间隙、销孔表面粗糙度和销孔指数型线内外半径增量变化对销孔轴承润滑特性的影响。结果表明:销孔结构对轴承的润滑特性有很大影响,销孔指数型线内半径增量的影响最大,而外半径增量的影响较小。最优参数组合为销孔轴承间隙0.021 mm、销孔表面粗糙度0.798μm、销孔指数型线内半径增量0.008 mm、销孔指数型线外半径增量0.010 mm,此时预测的最小油膜厚度为0.979μm;最大粗糙接触压力为249.406 MPa,与该方案下仿真值的相对误差小于5%。该研究优化方法效果好,且预测准确,可为后续的钢活塞销孔结构设计提供理论依据。

钢桁腹-混凝土组合箱梁局部外接节点静载试验及极限承载力计算研究

为了解钢桁腹-混凝土组合箱梁(顶、底板为混凝土结构,腹杆为钢结构)外接节点在水平荷载作用下的受力性能,以某钢桁腹-混凝土组合箱梁桥方案为背景,选取钢腹杆轴力最大节点设计、制作缩尺比为1∶3的钢桁腹-混凝土组合箱梁局部外接节点试件(由混凝土弦杆、方形钢腹杆、节点板组成,原混凝土板简化为混凝土弦杆)并开展静载试验,分析外接节点试件静载试验现象及破坏模式、荷载~位移曲线,以及混凝土弦杆、节点板和钢腹杆的应力分布规律,并基于有效宽度法及撕裂面法与最大强度准则提出外接节点极限承载力计算方法。结果表明:外接节点试件的破坏模式为节点板拉剪断裂失效,极限承载力为设计荷载的5.71倍,承载能力满足要求;混凝土弦杆压应力沿加载方向逐渐减小,沿竖直方向从上往下逐渐增大;节点板应力沿加载方向总体上先增后减,外露节点板是节点受力的关键构件;外接节点极限承载力由节点板和钢腹杆中较弱者控制,采用所提出的计算方法得到的外接节点极限承载力计算值与试验结果相差小于6%。

UHPC-部分充填窄幅钢箱组合梁抗弯性能

为研究UHPC-部分充填窄幅钢箱组合梁负弯矩下的抗弯性能,制作了6根试验梁探究翼板配筋率、UHPC钢纤维掺量及板厚对组合梁截面应变分布、破坏形式及极限承载力的影响。试验结果表明:3%钢纤维掺量的UHPC翼板开裂荷载比0、1%、2%钢纤维掺量的UHPC翼板分别大63.1%、29.8%、7.7%,说明钢纤维的加入,能够控制裂缝的发展,提高翼板的塑性,可有效改善破坏时的脆性现象。翼板配筋率提高1%时,抗弯承载力提高5.3%。用UHPC替代翼板1/2厚度的普通混凝土,开裂能力提高了56.2%,极限荷载提高了7%。基于简化塑性理论提出了组合梁抗弯承载力计算式,计算值与试验值吻合较好。

MiR-142-3p Regulates ILC1s by Targeting HMGB1 via the NF-κB Pathway in a Mouse Model of Early Pregnancy Loss

Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.

曲线钢-混组合箱梁桥车桥耦合振动及支座受力研究

曲线钢-混组合箱梁桥已经建设较多,在设计中因对其车桥耦合振动认识有限,相关支座反力因弯扭耦合效应引起的变化情况等尚未很好解决。基于ABAQUS有限元软件,利用傅里叶级数描述车辆平、竖曲线运动,实现了曲线梁桥车桥耦合振动的数值分析,并基于既有试验和理论成果验证了方法的准确性。以某4跨曲线钢-混组合箱梁桥为例,分析了车辆在内、外侧车道以不同速度行驶及超高条件下对桥梁支座反力的影响。结果表明:当车辆沿着曲线梁桥内侧或外侧车道行驶时,另一侧箱梁的外支座会出现脱空的现象。车速对支座峰值反力的影响较小,但可以通过较低的车速减轻支座脱空。超高对支座反力的影响较小,车辆沿着内侧车道行驶时,缓解了外侧箱梁部分支座脱空;沿着外侧车道行驶时,加重了内侧箱梁支座脱空。

Box-Behnken响应面优化超声联合双酶法提取当归地上部分总黄酮及其抗氧化活性研究

试验以当归废弃物(当归地上部分)为试验材料,在单因素试验基础上,设计Box-Behnken响应面试验,优化超声联合双酶法提取当归地上部分总黄酮的最佳提取工艺,采用DPPH和ABTS自由基法和FRAP法评价当归地上部分总黄酮的抗氧化活性。结果显示,利用超声联合双酶法提取当归地上部分总黄酮的最佳条件为乙醇浓度50%、液料比50∶1、超声时间41 min、双酶(纤维素酶∶果胶酶=2∶1)用量0.68 g/L、酶解时间93 min,此条件下,当归地上部分总黄酮提取率为8.18%。当归地上部分具有较高的抗氧化活性,在浓度为150 mg/L时,对DPPH自由基清除率为90.3%,略低于VC。在浓度为3 000 mg/L时,对ABTS清除率为88.6%,ABTS的IC50为DPPH IC50近30倍,FRAP抗氧化能力为4.64 (mg VC eq/g sample)。试验表明,当归地上部分的总黄酮具有抗氧化活性。

Y-box结合蛋白-1与肿瘤发生

Y-box结合蛋白(Y-box binding protein,YB)是一类广泛存在于多种生物中的蛋白质家族,是一类高度保守的顺式作用调控元件,也是一种广泛表达的转录因子,发挥重要的生物学功能。Y-box结合蛋白-1(Y-box binding protein-1,YB-1)是Y-box结合蛋白家族成员之一,参与基因的转录调节、翻译调控、维持mRNA的稳定性、mRNA选择性剪接、RNA的配对、DNA的修复、细胞增殖和再生等生物学功能。大量研究表明YB-1的异常表达与许多肿瘤,如:前列腺癌、乳腺癌、肺癌、宫颈癌、淋巴瘤、卵巢癌的发生发展具有相关性。对YB-1的研究为肿瘤的治疗提供了新的方向,并可能成为肿瘤诊治的一种新的标志物。

Box-Behnken设计毛细管液相色谱法拆分α-氨基酸对映体的研究

将L-脯氨酸通过3-缩水甘油丙基三甲氧基硅烷键合到球型多孔硅胶基质上,制备了手性配体交换色谱固定相。采用高压匀浆法将制备的填料填充到毛细管柱中,在自组装的毛细管液相色谱仪上对3种α-氨基酸对映体进行了拆分。利用Box-Behnken设计对影响因素(流动相中乙腈的体积分数、中心离子Cu2+的浓度、流速)进行优化,以最大保留时间和分离度为响应值,借助二次多元回归方程,建立了相应的数学模型。响应曲面图表明:DL-天冬酰胺在5%乙腈、5 mmol/L Cu2+、流速0.139μL/min,苏氨酸在10%乙腈、5mmol/L Cu2+、流速0.167μL/min,丝氨酸在5%乙腈、7 mmol/L Cu2+、流速0.167μL/min的条件下均能得到基线分离。表明通过所制备的手性固定相,采用配体交换色谱模式可获得拆分DL-氨基酸的理想效果。

Box-Behnken响应面设计法优化排脓散水提工艺

目的优选排脓散最佳水提工艺。方法采用Box-Behnken响应面设计法,以水提液中3个指标性成分(柚皮苷、新橙皮苷、芍药苷)的含量和出膏率的综合评价作为考察指标,考察料液比、浸泡时间、提取时间3个因素对提取工艺的影响。结果排脓散水提的最佳工艺条件为15倍水,浸泡67min,提取74min。结论 Box-Behnken实验设计法优化排脓散的水提工艺方法可行,预测性较好。

Powell dynamic identification of displacement parameters of indeterminate thin-walled curve box based on FCSE theory

The FCSE controlling equation of pinned thinwalled curve box was derived and the indeterminate problem of continuous thin-walled curve box with diaphragm was solved based on flexibility theory. With Bayesian statistical theory,dynamic Bayesian error function of displacement parameters of indeterminate curve box was founded. The corresponding formulas of dynamic Bayesian expectation and variance were deduced. Combined with one-dimensional Fibonacci automatic search scheme of optimal step size,the Powell optimization theory was utilized to research the stochastic identification of displacement parameters of indeterminate thin-walled curve box. Then the identification steps were presented in detail and the corresponding calculation procedure was compiled. Through some classic examples,it is obtained that stochastic performances of systematic parameters and systematic responses are simultaneously deliberated in dynamic Bayesian error function. The one-dimensional optimization problem of the optimal step size is solved by adopting Fibonacci search method. And the Powell identification of displacement parameters of indeterminate thin-walled curve box has satisfied numerical stability and convergence,which demonstrates that the presented method and the compiled procedure are correct and reliable.During parameters鈥？iterative processes,the Powell theory is irrelevant with the calculation of finite curve strip element（FCSE） partial differentiation,which proves high computation effciency of the studied method.

Full-scale model tests and nonlinear analysis of prestressed concrete simply supported box girders

Full-scale model tests were carried out on a 30 m span prestressed concrete box girder and a 20 m span prestressed concrete hollow slab. Failure models were prestressed reinforcement tensile failure and crashing of roof concrete, respectively. The ductility indexes of the box girder and hollow slab were 1.99 and 1.23, respectively, according to the energy viewpoint. Based on the horizontal section hypothesis, the nonlinear computation procedure was established using the limited banding law, and it could carry out the entire performance analysis including the unloading, mainly focusing on the ways to achieve the unloading curves computation through stress-strain, moment-curvature and load-displacement curves. Through the procedure, parameters that influence on the bearing capacity, deformation performance and ductility of the structures were analyzed. Those parameters were quantity of prestressed reinforcement and tension coefficients of prestressed reinforcement. From the analysis, some useful conclusions can be obtained.

木-钢组合箱梁抗弯性能试验及有限元对比分析

【目的】为促进木结构在大型工程中的应用范围,本研究提出了一种以落叶松胶合木板作为上下翼板、焊接冷弯薄壁槽钢作为腹板并通过螺栓连接的木-钢组合箱梁。【方法】采用分级加载的方式分别对3根木-钢组合箱梁和1根木箱梁进行弯曲加载试验,观测组合箱梁和木箱梁在荷载作用下翼缘和腹板的应变变化、挠度的发展、破坏过程及形态变化,研究其抗弯极限承载力和抗弯刚度等力学性能,并基于ABAQUS建立了有限元模型对组合箱梁及胶合木箱梁进行数值模拟分析。【结果】组合箱梁的翼板与腹板组合性能较好,最终均为下翼缘木板受拉断裂的破坏模式,同时伴随着钢材局部屈曲和上翼缘木材的局部劈裂等破坏;木箱梁为腹板顺纹剪切破坏。相比胶合木箱梁,组合箱梁的抗弯极限承载能力平均提升了30.33%,但初始刚度平均降低了34.42%;根据翼板横向应变分布计算了木箱梁和木-钢组合箱梁上翼缘的剪力滞系数以及有效分布宽度。有限元模型与试验结果吻合良好,能有效预测木-钢组合箱梁及胶合木箱梁的弯曲性能。【结论】木-钢组合箱梁充分发挥了木材和钢材的力学性能,相比于纯木箱梁,抗弯承载力提升显著,说明木-钢组合箱梁是一种科学合理的组合形式,研究成果可为木-钢组合箱梁的后续研究提供一定的理论基础。

MiR-183-5p promotes the progression of non-small cell lung cancer through targeted regulation of FOXO1

Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells.Methods NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative resection.Several cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and 95-D.miR-183-5p and FOXO1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was analyzed.The miR-183-5p-mediated regulation of FOXO1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FOXO1 groups.Cell proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,respectively.The expression of EMT-related proteins in the cells was analyzed by western blotting.The effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude mice.Results miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FOXO1 mRNA expression was significantly down-regulated.There was a significant negative correlation between miR-183-5p and FOXO1 mRNA in NSCLC tissues(P<0.05).Additionally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<0.05).miR-183-5p targeted and inhibited FOXO1 expression.Compared to the anti-miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the anti-miR-183-5p group,whereas the protein expression of E-cadherin andα-catenin and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion were significantly lower in the anti-miR-183-5p group(P<0.05).Compared to the miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells in the miR-183-5p group were significantly higher,whereas the E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly lower;furthermore,the frequency of colony formation and invasion were significantly higher in the miR-183-5p group(P<0.05).Compared with the miR-183-5p+pcDNA3.1 group,the OD value,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group,whereas E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion was significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group(P<0.05).Overall,silencing miR-185-3p inhibited the growth of transplanted tumors and promoted FOXO1 expression.Conclusion Overexpression of miR-183-5p can inhibit apoptosis and promote the proliferation,migration,invasion,and EMT,of NSCLC cells by down-regulating FOXO1 expression.

高铁轴承钢的技术质量特性识别及重点研制方向

从相关标准规范、主要故障模式及在用轴承技术状态等多个维度对高铁轴箱轴承用钢的技术性能要求进行了梳理,进而对其核心技术质量特性进行了识别,即“强韧性、抗疲劳、耐磨性”,并对自主化研制高铁轴承钢的技术路线及改进方向给出了建议。在现阶段,集成渗碳钢与“真空脱气+电渣重熔”冶炼方法的优势,是最具合理性与可行性的解决方案。

三星堆古蜀文化遗址博物馆大型螺旋坡道结构设计与分析

三星堆古蜀文化遗址博物馆中庭的螺旋坡道采用钢箱梁结构体系,最大半径28.7m,水平投影长度118.5m。介绍了大型螺旋坡道结构体系选型和边界条件的对比过程,采用通用计算软件MIDAS Gen建立多尺度整体模型考虑螺旋坡道和主体结构的相互作用,通过静力分析和反应谱分析考察螺旋坡道在不同荷载作用下的承载能力和变形,并采用有限元软件ANSYS建立分析模型进行连续倒塌分析。结果表明,螺旋坡道采用钢箱梁结构具有较好的整体刚度,静力荷载和地震作用下坡道的应力和变形均满足规范要求,并具有较好的抗连续倒塌能力。

探究钢-混凝土组合箱梁桥的荷载试验

随着近年来高速公路里程的迅速增长,越来越多的新材料、新工艺不断应用于高速公路的建设中,钢-混凝土组合结构能够充分发挥材料各自的优良特性,也被广泛运用于土木建设领域。为评估新建钢-混凝土组合箱梁桥的承载能力,依据《公路工程质量检验评定标准》应进行荷载试验。钢-混凝土组合结构作为全新的结构形式,对其开展荷载试验评定技术研究,对于确保同类桥梁结构的安全使用、完善桥梁的荷载试验理论都具有重要的现实意义。