The significant demand for high quality food has motivated us to adopt appropriate processing methods to improve the food nutritional quality and flavors.In this study,the effects of five drying methods,namely,pulsed ...The significant demand for high quality food has motivated us to adopt appropriate processing methods to improve the food nutritional quality and flavors.In this study,the effects of five drying methods,namely,pulsed vacuum drying(PVD),freeze drying(FD),infrared drying(IRD),hot-air drying(HAD)and sun drying(SD)on free amino acids(FAAs),α-dicarbonyl compounds(α-DCs)and volatile compounds(VOCs)in rape bee pollen(RBP)were determined.The results showed that FD significantly released the essential amino acids(EAAs)compared with fresh samples while SD caused the highest loss.Glucosone was the dominantα-DCs in RBP and the highest loss was observed after PVD.Aldehydes were the dominant volatiles of RBP and SD samples contained more new volatile substances(especially aldehydes)than the other four drying methods.Comprehensively,FD and PVD would be potential methods to effectively reduce the quality deterioration of RBP in the drying process.展开更多
The nutrient digestion,absorption and biological activity of bee pollen may be limited due to the complex pollen wall.Here,the effect of superfine grinding technology on the release of nutrients from bee pollen were i...The nutrient digestion,absorption and biological activity of bee pollen may be limited due to the complex pollen wall.Here,the effect of superfine grinding technology on the release of nutrients from bee pollen were investigated,and their antioxidant activities and in vitro digestion were explored in this study.Results showed that the content of nutrients in bee pollen increased after wall disruption.Among them,fat content increased by 22.55%-8.31%,protein content increased by 0.54%-4.91%,starch content increased by 36.31%-48.64%,soluble sugar content increased by 20.57%-29.67%,total phenolic acid content increased by 11.73%-86.98%and total flavonoids content increased by 14.29%-24.79%.At the same time,the antioxidant activity increased by 14.84%-46.00%.Furthermore,the active components such as phenolic compounds in the wall-disruption bee pollen were more readily to be released during the in vitro digestion,and easier to be absorbed because of their higher bioaccessibility.Antioxidant activities during in vitro digestion were also improved in walldisruption bee pollen.These findings provide evidence that bee pollen wall disruption was suggested,thus,it is more conducive to exerting the value of bee pollen in functional foods.展开更多
Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lac...Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.展开更多
Bee pollen has potential in preventing metabolic syndrome(MetS).The present study aimed to investigate the effect of yeast-fermented wall-broken bee pollen(YB)intervention on ICR mice with MetS induced with a high-fat...Bee pollen has potential in preventing metabolic syndrome(MetS).The present study aimed to investigate the effect of yeast-fermented wall-broken bee pollen(YB)intervention on ICR mice with MetS induced with a high-fat(HF)diet.After YB intervention in mice for 16 weeks,the effect on alleviating MetS was evaluated based on MetS serum parameters,hepatic oxidant status markers and gut microbial populations.The results of animal experiment showed that YB intervention attenuated MetS.Based on multivariate statistical analysis results,YB treatment signifi cantly increased glutathione S-transferase(GST)and catalase(CAT)activities and decreased the malondialdehyde(MDA)level in the liver.Further investigation showed that YB restored the Nrf-2-Keap-1 pathway to alleviate oxidative stress.Additionally,gut microbial community analysis revealed that YB restored the increase in the Firmicutes to Bacteroidetes(F/B)ratio(6.94 for the HF group and 3.74 for HF+YB group)and improved Lactobacillus and Lactococcus abundance induced by the HF diet.Overall,YB improved function and prevented MetS by modulating the gut microbiota and alleviating oxidative stress.展开更多
[Objective]The paper was to verify whether chestnut bee pollen can be used as feed for breeding chickens and its appropriate addition proportion.[Method]A total of 92444-week-old Ross 308 broiler breeders of parent ge...[Objective]The paper was to verify whether chestnut bee pollen can be used as feed for breeding chickens and its appropriate addition proportion.[Method]A total of 92444-week-old Ross 308 broiler breeders of parent generation with a male to female ratio of 1∶10 were randomly divided into 4 groups,with 3 replicates in each group and 7 cocks and 70 hens in each replicate.The broiler breeders in the control group were fed with the basal diet,and those in experimental groups were fed with the diets supplemented with 0.5%,1.0%and 1.5%chestnut bee pollen.The feeding trial lasted 35 d.[Result]Addition of chestnut bee pollen at different proportions improved the laying rate of breeder hens in different degrees.The experimental group supplemented with 1.5%chestnut bee pollen increased the laying rate by 9.1%,reduced the rate of unqualified eggs(broken eggs,cracked eggs,thin shelled eggs and hard shelled eggs)by 71.4%,increased the fertilization rate of hatching eggs and healthy chick rate by 4.4%and 5.2%,and improved the concentrations of testosterone(T),follicle stimulating hormone(FSH)and luteinizing hormone(LH)in breeder cocks by 60.8%,32.3%and 61.4%,respectively.Statistical analysis showed that the results were significantly different.[Conclusion]Addition of chestnut bee pollen at certain concentration can improve the reproductive performance of breeder cocks and breeder hens,and improve the body immunity.展开更多
Objective: To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract(BPE) of Malaysian stingless bee [Lepidotrigona terminata(L. terminata)]and its synergistic effect with cisplatin(a c...Objective: To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract(BPE) of Malaysian stingless bee [Lepidotrigona terminata(L. terminata)]and its synergistic effect with cisplatin(a chemotherapeutic drug) on MCF-7 cancer cell line.Methods: The antioxidant activity of BPE from L. terminata was measured by using1,1-diphenyl-2-picrylhydrazyl radical(DPPH) assay. Antiproliferative activity at different concentrations of BPE and cisplatin was determined through using MTT assay on MCF-7 and L929 cell lines. An interaction effect(synergistic, additive and antagonistic) between BPE and cisplatin was determined by Compu Syn software based on MTT assay data.Results: The EC50(50% decrement of DPPH inhibition) of BPE was 0.5 mg/m L.L. terminata BPE exhibited antiproliferative activity on both cancer and normal cell lines.The IC50(concentration of drug that was required for 50% of cell inhibition in vitro) of BPE on MCF-7 was 15 mg/m L whereas in normal cell line L929 was 26 mg/m L. The IC50 for cisplatin on MCF-7 was 20 mmol/L. The combination effect of BPE and cisplatin on MCF-7 cells showed that BPE at 15 mg/m L was able to potentiate the inhibitory effect of cisplatin at all different concentrations(2.5–20.0 mg/m L). The average of cancer cells inhibition which was potentiated by BPE was around 50%. A combination index values of less than 1 reported in the Compu Syn software further proved the synergistic effect between BPE and cisplatin, suggesting that BPE was working synergistically with cisplatin.Conclusions: Our study therefore suggested that BPE of Malaysian stingless bee,L. terminata is a potential chemopreventive agent and can be used as a supplementary treatment for chemotherapy drugs. BPE might be able to be used to potentiate the effect of chemotherapy drugs with the possibility to reduce the required dose of the drugs. The molecular mechanisms of how the BPE exerts antiproliferative activity will be a much interesting area to look for in future studies.展开更多
OBJECTIVE: This study was designed to investigate the nutraceutical potential of monofloral Indian mustard bee pollen (MIMBP). METHODS: The nutritional value of MIMBP was examined in terms of proteins, fats, carbo...OBJECTIVE: This study was designed to investigate the nutraceutical potential of monofloral Indian mustard bee pollen (MIMBP). METHODS: The nutritional value of MIMBP was examined in terms of proteins, fats, carbohydrates, and energy value. Its chemical composition in terms of total polyphenol and flavonoid content was determined. MIMBP was screened for free flavonoid aglycones by developing and validating a high-performance liquid chromatography-photo diode array (HPLC-PDA) method. MIMBP was analyzed for in vitro antioxidant effect in terms of 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical-scavenging activity. RESULTS: MIMBP was found to be comprised of proteins ((182.2±5.9) g/kg), fats ((137.7±6.8) g/kg) and carbohydrates ((560.6±17.4) g/kg), which result in its high energy value ((17 616.7±78.6) kJ/kg). MIMBP was found to contain polyphenols ((18 286.1±374.0) mg gallic acid equivalent/kg) and flavonoids ((1 223.5±53.1) mg quercetin equivalent/kg). The HPLC-PDA analysis revealed the presence of kaempferol ((65.4±0.5) mg/kg) and quercefin ((51.4±0.4) mg/kg) in MIMBP, which can be used as markers for determining the quality of bee pollen. The MIMBP extract showed DPPH free radical-scavenging activity with a half maximal inhibitory concentration of 54.79 μg/mL. CONCLUSION: The MIMBP was found to be a rich source of nutrients providing high caloric value, which makes it a candidate for a potential nutraceutical agent. The study also illustrated the high antioxidant content of MIMBP, especially in the principle polyphenols and flavonoids, which suggests its potential role in the prevention of free radical-implicated diseases. The DPPH-scavenging effect of MIMBP further confirmed its antioxidant potential. Additionally, we developed a simple, specific and accurate HPLC-PDA method for the identification and quantification of free flavonoid aglycones. This can be applied in future screenings of the quality of pollen collected by honeybees.展开更多
Waste utilization is not only the protection of the environment and the practice of green chemistry,but also one of the ways to develop new materials.Herein,we report two biomass carbon dots which prepared from bee po...Waste utilization is not only the protection of the environment and the practice of green chemistry,but also one of the ways to develop new materials.Herein,we report two biomass carbon dots which prepared from bee pollen waste by one-step hydrothermal method.The new two carbon dots were used in sensing,cell imaging and plant growth regulation.The differences in the structure and properties of the two carbon dots were evaluated by TEM,XPS,TG and various spectroscopic methods.Both two carbon dots contain abundant functional groups,polyatomic doping,excellent water solubility and stable photoluminescence.Due to these good properties,we have demonstrated its versatile applications in Fe3+sensing,cell imaging and plant growth regulation.It shows sensitive and specific Fe^(3+) responsiveness and good biocompatibility.This research provides a green and simple method for the recycling and reuse of bee pollen waste,and also provides a reference for the application of biomass carbon dots.展开更多
Background Flavonoids from fruits, vegetables and plants have been widely studied on their improving lipidemia, anti-inflammation, anti-platelet aggregation and anti-oxidative activities. However, t know if flavonoids...Background Flavonoids from fruits, vegetables and plants have been widely studied on their improving lipidemia, anti-inflammation, anti-platelet aggregation and anti-oxidative activities. However, t know if flavonoids from rape bee pollen have the same effects in patients with dyslipidemia (DL), effects of we don' type 2 diabetes mellitus(T2DM), and cerebral infarction(CI). Methods The study involved with patients selected and divided into 4 groups, 30 cases of DL, of T2DM, of CI and health control (HC) for each. Forty mg flavonoids from rape bee pollen administrated orally twice a day for two months. Blood sugar (BG), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low white blood-cell counts (WBC), platelet counts (PLT), high-sens malondialdehyde (MDA) were analyzed in four groups before and after density lipoprotein-cholesterol (LDL-C), itivity C-reactive protein (hsCRP) and taking flavonoids for two months. Results (1) Basic levels of TC, BG, WBC, PLT and hsCRP in groups of T2DM, CI, and TG and MDA in groups of DL, PLT, T2DM and CI were significantly higher than those in HC (P 〈 0.05-0.001). (2) The levels of hsCRP and MDA were significantly decreased, flavonoids for two months (P 〈 0.05-0.001) in group of while HDL-C was remarkably increased DL, T2MD, and CL These indexes were not the group of HC after taking flavonoids (P 〉 0.05). Conclusions The results could indieat disturbance, inflammation, and high oxidative stress in patients with dyslipidemisa, type 2 diabet TG, WBC, after taking different in e metabolic es mellitus, and cerebral infarction. The flavonoids from rape bee pollen may have effects of antioxidant activity, improving lipids, and anti-inflammation on these patients.展开更多
To explore the difference in pollen harvest between the western honey bee Apis mellifera and a native eastern honey bee A.cerana in the Zoige alpine meadows in the northeastern part of the QinghaiTibetan Plateau,we in...To explore the difference in pollen harvest between the western honey bee Apis mellifera and a native eastern honey bee A.cerana in the Zoige alpine meadows in the northeastern part of the QinghaiTibetan Plateau,we investigated species diversity of the harvested pollen and the amount of harvested pollen per bee for both species,and calculated the niche overlap(in terms of similarity in harvested pollen)between the two bee species during the flowering season from June to August 2016.Results showed that the species diversity of the harvested pollen was indistinguishable between the two bee species.Nevertheless,A.mellifera carried more(although not significant)pollens per bee than A.cerana.Moreover,pollen composition differed between the two bee species:A.mellifera mainly foraged on Anemone rivularis,Saussurea nigrescens and Anemone trullifolia,while A.cerana foraged on Anemone rivularis,Stellera chamaejasme,and Pedicularis longiflora.Consistently,the niche overlap between the two honeybee species was particularly small in several observations.Our results indicate a niche separation in pollen resource between the two honeybee species in a Tibetan alpine meadow.展开更多
基金supported by National Natural Science Foundation of China(Nos.31871861 and 31501548)The Apicultural Industry Technology System(NCYTI-43-KXJ17)The Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2015-IAR)。
文摘The significant demand for high quality food has motivated us to adopt appropriate processing methods to improve the food nutritional quality and flavors.In this study,the effects of five drying methods,namely,pulsed vacuum drying(PVD),freeze drying(FD),infrared drying(IRD),hot-air drying(HAD)and sun drying(SD)on free amino acids(FAAs),α-dicarbonyl compounds(α-DCs)and volatile compounds(VOCs)in rape bee pollen(RBP)were determined.The results showed that FD significantly released the essential amino acids(EAAs)compared with fresh samples while SD caused the highest loss.Glucosone was the dominantα-DCs in RBP and the highest loss was observed after PVD.Aldehydes were the dominant volatiles of RBP and SD samples contained more new volatile substances(especially aldehydes)than the other four drying methods.Comprehensively,FD and PVD would be potential methods to effectively reduce the quality deterioration of RBP in the drying process.
基金the Program of State Key Laboratory of Food Science and Technology,Nanchang University (SKLF-ZZB-202119)。
文摘The nutrient digestion,absorption and biological activity of bee pollen may be limited due to the complex pollen wall.Here,the effect of superfine grinding technology on the release of nutrients from bee pollen were investigated,and their antioxidant activities and in vitro digestion were explored in this study.Results showed that the content of nutrients in bee pollen increased after wall disruption.Among them,fat content increased by 22.55%-8.31%,protein content increased by 0.54%-4.91%,starch content increased by 36.31%-48.64%,soluble sugar content increased by 20.57%-29.67%,total phenolic acid content increased by 11.73%-86.98%and total flavonoids content increased by 14.29%-24.79%.At the same time,the antioxidant activity increased by 14.84%-46.00%.Furthermore,the active components such as phenolic compounds in the wall-disruption bee pollen were more readily to be released during the in vitro digestion,and easier to be absorbed because of their higher bioaccessibility.Antioxidant activities during in vitro digestion were also improved in walldisruption bee pollen.These findings provide evidence that bee pollen wall disruption was suggested,thus,it is more conducive to exerting the value of bee pollen in functional foods.
基金supported by the National Natural Science Foundation of China(32102605)the Agricultural Science and Technology Innovation Program under Grant(CAAS-ASTIP-2020-IAR)。
文摘Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.
基金This study was supported by the National Natural Science Foundation of China(No.31972628 and 31472155)a special fund(NYCYTX-43)the fund of the Beijing Laboratory for Food Quality and Safety,Beijing Technology and Business University.
文摘Bee pollen has potential in preventing metabolic syndrome(MetS).The present study aimed to investigate the effect of yeast-fermented wall-broken bee pollen(YB)intervention on ICR mice with MetS induced with a high-fat(HF)diet.After YB intervention in mice for 16 weeks,the effect on alleviating MetS was evaluated based on MetS serum parameters,hepatic oxidant status markers and gut microbial populations.The results of animal experiment showed that YB intervention attenuated MetS.Based on multivariate statistical analysis results,YB treatment signifi cantly increased glutathione S-transferase(GST)and catalase(CAT)activities and decreased the malondialdehyde(MDA)level in the liver.Further investigation showed that YB restored the Nrf-2-Keap-1 pathway to alleviate oxidative stress.Additionally,gut microbial community analysis revealed that YB restored the increase in the Firmicutes to Bacteroidetes(F/B)ratio(6.94 for the HF group and 3.74 for HF+YB group)and improved Lactobacillus and Lactococcus abundance induced by the HF diet.Overall,YB improved function and prevented MetS by modulating the gut microbiota and alleviating oxidative stress.
文摘[Objective]The paper was to verify whether chestnut bee pollen can be used as feed for breeding chickens and its appropriate addition proportion.[Method]A total of 92444-week-old Ross 308 broiler breeders of parent generation with a male to female ratio of 1∶10 were randomly divided into 4 groups,with 3 replicates in each group and 7 cocks and 70 hens in each replicate.The broiler breeders in the control group were fed with the basal diet,and those in experimental groups were fed with the diets supplemented with 0.5%,1.0%and 1.5%chestnut bee pollen.The feeding trial lasted 35 d.[Result]Addition of chestnut bee pollen at different proportions improved the laying rate of breeder hens in different degrees.The experimental group supplemented with 1.5%chestnut bee pollen increased the laying rate by 9.1%,reduced the rate of unqualified eggs(broken eggs,cracked eggs,thin shelled eggs and hard shelled eggs)by 71.4%,increased the fertilization rate of hatching eggs and healthy chick rate by 4.4%and 5.2%,and improved the concentrations of testosterone(T),follicle stimulating hormone(FSH)and luteinizing hormone(LH)in breeder cocks by 60.8%,32.3%and 61.4%,respectively.Statistical analysis showed that the results were significantly different.[Conclusion]Addition of chestnut bee pollen at certain concentration can improve the reproductive performance of breeder cocks and breeder hens,and improve the body immunity.
基金Supported by Fundamental Research Grant Scheme,Ministry of Education Malaysia(Grant No:FRGS/2/2013/SKK01/USM/03/3)
文摘Objective: To evaluate the antioxidant and antiproliferative effect of methanolic bee pollen extract(BPE) of Malaysian stingless bee [Lepidotrigona terminata(L. terminata)]and its synergistic effect with cisplatin(a chemotherapeutic drug) on MCF-7 cancer cell line.Methods: The antioxidant activity of BPE from L. terminata was measured by using1,1-diphenyl-2-picrylhydrazyl radical(DPPH) assay. Antiproliferative activity at different concentrations of BPE and cisplatin was determined through using MTT assay on MCF-7 and L929 cell lines. An interaction effect(synergistic, additive and antagonistic) between BPE and cisplatin was determined by Compu Syn software based on MTT assay data.Results: The EC50(50% decrement of DPPH inhibition) of BPE was 0.5 mg/m L.L. terminata BPE exhibited antiproliferative activity on both cancer and normal cell lines.The IC50(concentration of drug that was required for 50% of cell inhibition in vitro) of BPE on MCF-7 was 15 mg/m L whereas in normal cell line L929 was 26 mg/m L. The IC50 for cisplatin on MCF-7 was 20 mmol/L. The combination effect of BPE and cisplatin on MCF-7 cells showed that BPE at 15 mg/m L was able to potentiate the inhibitory effect of cisplatin at all different concentrations(2.5–20.0 mg/m L). The average of cancer cells inhibition which was potentiated by BPE was around 50%. A combination index values of less than 1 reported in the Compu Syn software further proved the synergistic effect between BPE and cisplatin, suggesting that BPE was working synergistically with cisplatin.Conclusions: Our study therefore suggested that BPE of Malaysian stingless bee,L. terminata is a potential chemopreventive agent and can be used as a supplementary treatment for chemotherapy drugs. BPE might be able to be used to potentiate the effect of chemotherapy drugs with the possibility to reduce the required dose of the drugs. The molecular mechanisms of how the BPE exerts antiproliferative activity will be a much interesting area to look for in future studies.
基金the University Grants Commission, India for providing financial assistance for the work.
文摘OBJECTIVE: This study was designed to investigate the nutraceutical potential of monofloral Indian mustard bee pollen (MIMBP). METHODS: The nutritional value of MIMBP was examined in terms of proteins, fats, carbohydrates, and energy value. Its chemical composition in terms of total polyphenol and flavonoid content was determined. MIMBP was screened for free flavonoid aglycones by developing and validating a high-performance liquid chromatography-photo diode array (HPLC-PDA) method. MIMBP was analyzed for in vitro antioxidant effect in terms of 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical-scavenging activity. RESULTS: MIMBP was found to be comprised of proteins ((182.2±5.9) g/kg), fats ((137.7±6.8) g/kg) and carbohydrates ((560.6±17.4) g/kg), which result in its high energy value ((17 616.7±78.6) kJ/kg). MIMBP was found to contain polyphenols ((18 286.1±374.0) mg gallic acid equivalent/kg) and flavonoids ((1 223.5±53.1) mg quercetin equivalent/kg). The HPLC-PDA analysis revealed the presence of kaempferol ((65.4±0.5) mg/kg) and quercefin ((51.4±0.4) mg/kg) in MIMBP, which can be used as markers for determining the quality of bee pollen. The MIMBP extract showed DPPH free radical-scavenging activity with a half maximal inhibitory concentration of 54.79 μg/mL. CONCLUSION: The MIMBP was found to be a rich source of nutrients providing high caloric value, which makes it a candidate for a potential nutraceutical agent. The study also illustrated the high antioxidant content of MIMBP, especially in the principle polyphenols and flavonoids, which suggests its potential role in the prevention of free radical-implicated diseases. The DPPH-scavenging effect of MIMBP further confirmed its antioxidant potential. Additionally, we developed a simple, specific and accurate HPLC-PDA method for the identification and quantification of free flavonoid aglycones. This can be applied in future screenings of the quality of pollen collected by honeybees.
基金the National Natural Science Foundation of China(No.21877082)Sichuan Science and Technology Program(No.2021YFG0291)+2 种基金International Science and Technology Innovation Cooperation Project of Sichuan Province(No.21GJHZ0092)Sichuan Province Innovation and Entrepreneurship Project(No.S202110650037)the Undergraduate Scientific and Technological Innovation Project(No.2020029),Xihua University。
文摘Waste utilization is not only the protection of the environment and the practice of green chemistry,but also one of the ways to develop new materials.Herein,we report two biomass carbon dots which prepared from bee pollen waste by one-step hydrothermal method.The new two carbon dots were used in sensing,cell imaging and plant growth regulation.The differences in the structure and properties of the two carbon dots were evaluated by TEM,XPS,TG and various spectroscopic methods.Both two carbon dots contain abundant functional groups,polyatomic doping,excellent water solubility and stable photoluminescence.Due to these good properties,we have demonstrated its versatile applications in Fe3+sensing,cell imaging and plant growth regulation.It shows sensitive and specific Fe^(3+) responsiveness and good biocompatibility.This research provides a green and simple method for the recycling and reuse of bee pollen waste,and also provides a reference for the application of biomass carbon dots.
基金supported by The Capital Development Fund(2005-SF-Ⅱ-007)
文摘Background Flavonoids from fruits, vegetables and plants have been widely studied on their improving lipidemia, anti-inflammation, anti-platelet aggregation and anti-oxidative activities. However, t know if flavonoids from rape bee pollen have the same effects in patients with dyslipidemia (DL), effects of we don' type 2 diabetes mellitus(T2DM), and cerebral infarction(CI). Methods The study involved with patients selected and divided into 4 groups, 30 cases of DL, of T2DM, of CI and health control (HC) for each. Forty mg flavonoids from rape bee pollen administrated orally twice a day for two months. Blood sugar (BG), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low white blood-cell counts (WBC), platelet counts (PLT), high-sens malondialdehyde (MDA) were analyzed in four groups before and after density lipoprotein-cholesterol (LDL-C), itivity C-reactive protein (hsCRP) and taking flavonoids for two months. Results (1) Basic levels of TC, BG, WBC, PLT and hsCRP in groups of T2DM, CI, and TG and MDA in groups of DL, PLT, T2DM and CI were significantly higher than those in HC (P 〈 0.05-0.001). (2) The levels of hsCRP and MDA were significantly decreased, flavonoids for two months (P 〈 0.05-0.001) in group of while HDL-C was remarkably increased DL, T2MD, and CL These indexes were not the group of HC after taking flavonoids (P 〉 0.05). Conclusions The results could indieat disturbance, inflammation, and high oxidative stress in patients with dyslipidemisa, type 2 diabet TG, WBC, after taking different in e metabolic es mellitus, and cerebral infarction. The flavonoids from rape bee pollen may have effects of antioxidant activity, improving lipids, and anti-inflammation on these patients.
基金financially supported by the National Natural Science Foundation of China (31470482)the Fundamental Research Funds for the Central Universities
文摘To explore the difference in pollen harvest between the western honey bee Apis mellifera and a native eastern honey bee A.cerana in the Zoige alpine meadows in the northeastern part of the QinghaiTibetan Plateau,we investigated species diversity of the harvested pollen and the amount of harvested pollen per bee for both species,and calculated the niche overlap(in terms of similarity in harvested pollen)between the two bee species during the flowering season from June to August 2016.Results showed that the species diversity of the harvested pollen was indistinguishable between the two bee species.Nevertheless,A.mellifera carried more(although not significant)pollens per bee than A.cerana.Moreover,pollen composition differed between the two bee species:A.mellifera mainly foraged on Anemone rivularis,Saussurea nigrescens and Anemone trullifolia,while A.cerana foraged on Anemone rivularis,Stellera chamaejasme,and Pedicularis longiflora.Consistently,the niche overlap between the two honeybee species was particularly small in several observations.Our results indicate a niche separation in pollen resource between the two honeybee species in a Tibetan alpine meadow.