Molecular Characterization of Coxsackievirus B1 Strains Isolated from Patients with Hand Foot and Mouth Disease in Yunnan,Southwest China

Coxsackievirus(CV)B belongs to the species Enterovirus B,genus Enterovirus of the family Picornaviridae.Enterovirus B(EV-B)includes 63 serotypes:CVB1-6;CVA9;echoviruses E1-7,9,11-21,24-27,and 29-33;EV-B69,EV-B 73-75,EV-B77-88,EV-B 93,EV-B 97-101,EV-B 106-107.

Hans G.Beger:one of the most renowned general and gastrointestinal surgeons in Germany

Professor Beger is one of the most renowned general and gastrointestinal surgeons in Germany.His worldwide reputation is based on his expertise in surgery of the pancreas.He belongs to the group of the 100most cited surgeons worldwide.

The Quest for Haven in Sister Carrie

This essay analyses Theodore Dreiser’s Sister Carrie through the lens of cultural studies,examining the portrayal of domesticity and the quest for an ideal home in late-nineteenth-century America.It explores the complex relationships between the main characters,Carrie and Hurstwood,and their families,as well as their struggles with materialism and alienation.Through close analysis of societal context,the characters’familial backgrounds and emotional yearnings,the essay delves into the characters’longing for stability and belonging and their journey toward understanding the true essence of dwelling amidst the changing urban landscape.It argues that Dreiser’s novel critiques the superficial pursuit of material comforts and highlights the importance of introspection and self-discovery in finding lasting happiness amidst societal turmoil.Ultimately,through the prism of domesticity,the essay delves into broader themes of identity,belonging,and existential fulfillment in a heartless society marked by materialism and alienation.

The combination of DNA methylation and positive regulation of anthocyanin biosynthesis by MYB and bHLH transcription factors contributes to the petal blotch formation in Xibei tree peony

Xibei tree peony is a distinctive cultivar group that features red–purple blotches in petals.Interestingly,the pigmentations of blotches and non-blotches are largely independent of one another.The underlying molecular mechanism had attracted lots of attention from investigators,but was still uncertain.Our present work demonstrates the factors that are closely related to blotch formation in Paeonia rockii‘Shu Sheng Peng Mo’.Non-blotch pigmentation is prevented by the silencing of anthocyanin structural genes,among which PrF3H,PrDFR,and PrANS are the three major genes.We characterized two R2R3-MYBs as the key transcription factors that control the early and late anthocyanin biosynthetic pathways.PrMYBa1,which belongs to MYB subgroup 7(SG7)was found to activate the early biosynthetic gene(EBG)PrF3H by interacting with SG5 member PrMYBa2 to form an‘MM’complex.The SG6 member PrMYBa3 interacts with two SG5(IIIf)bHLHs to synergistically activate the late biosynthetic genes(LBGs)PrDFR and PrANS,which is essential for anthocyanin accumulation in petal blotches.The comparison of methylation levels of the PrANS and PrF3H promoters between blotch and non-blotch indicated a correlation between hypermethylation and gene silencing.The methylation dynamics of PrANS promoter during flower development revealed a potential early demethylating reaction,which may have contributed to the particular expression of PrANS solely in the blotch area.We suggest that the formation of petal blotch may be highly associated with the cooperation of transcriptional activation and DNA methylation of structural gene promoters.

The Pathogenic Characteristics of the Initial Three Mpox Cases in Hunan Province, China

Mpox virus (MPXV) belongs to the family Poxviridae,subfamily Chordopoxvirinae,and genus Orthopoxvirus,which also includes Variola virus(VARV),Cowpox virus (CPXV),and Vaccinia virus(VACV).Clinical symptoms of mpox are similar to smallpox but are relatively milder^([1]).MPXV was first isolated from crab-eating monkeys in the tropical rainforests of Africa in 1958^([2]).The first human case of MPXV infection was reported in the Congo in1970^([3]).Following the eradication of smallpox in1977,mpox has emerged as one of the prominent viral members of the Poxviridae family,significantly impacting public health^([4]),mainly prevalent in Central and West Africa.Starting from May 2022.

Genome assembly of the snow lotus species Saussurea involucrata provides insights into acacetin and rutin biosynthesis and tolerance to an alpine environment

Dear Editor,Understanding the evolution and survival mechanisms of endangered wild medicinal herbs is crucial for their cultivation,utilization,and conservation.The snow lotus species Saussurea involucrata(Kar.&Kir.)Sch.Bip.(2n=32)(i.e.the well-known Tianshan snow lotus)which belongs to the eudicot family Asteraceae,is a famous traditional Chinese medicinal herb having anti-inflammatory.

The Philosophical Origin Behind the American Perception of China

As the most powerful country in the world,the United States claims to be the most advanced nation in natural sciences,humanities,and social sciences.However,why does it repeatedly commit mistakes on common sense issues such as“the world belongs to the entire international community”and“making an enemy out of China benefits no one”?An important reason lies in the philosophical origin of its China perception.

图片新闻

Chinese theatre(戏曲,Xiqu),also known as Chinese operas,not only belongs to China,but also belongs to the world.Since the 18th century,it has been engaged in dialogue,exchange and interaction with the world drama in various forms.With the pace of the overseas dissemination of Chinese theatre art,there will be more platforms for dialogue between Chinese opera and the world.and more communication methods.

FLAWED NARRATIVES OF CHINA AND TECHNOLOGY HARM AMERICAN AUDIENCES

The back half of the 20th century belonged to the United States.One reason is that America’s colleges and universities produced undergraduate,graduate,and doctoral graduates who,with increasing rapidity and sophistication,turned out one new technology tool after another.Using these and other advantages,the U.S.became the world’s powerhouse,outpacing its allies and adversaries.

郯庐断裂带张八岭隆起南段肥东群石榴角闪岩变质P-T演化史对其构造属性的制约

通过对张八岭隆起南端肥东群中磁铁石榴铁闪石岩的岩相学、矿物成分分析和热力学评价,并结合野外地质填图和共生围岩分析表明,该岩石经历了5期变质作用,展现了顺时针的P-T演化特征,早期阶段表现为近等压升温,晚期阶段为降温降压的过程,其峰期变质平均P-T条件为:524℃和0.49GPa。结合地质背景和郯庐断裂带的演化特征分析,早期过程可能反映的扬子基底所经历的区域变质过程,晚期过程可能是由于郯庐断裂的后期抬升分量所致,不具有造山带的P-T演化特征。对比前人对宿松群的变质演化历史的研究,肥东群和宿松群峰期变质压力差可达0.8GPa,表明两者属于不同变质级别的块体。因而,肥东群和宿松群不能作为被郯庐断裂左行错开的标志层。由此表明,张八岭隆起南段所出露的肥东群变质杂岩,属于扬子板块上的变质基底,郯庐断裂带活动中将其剥露地表,并没有经历过大别造山带的变质作用。

基于马斯洛需求层次理论的灾后心理危机干预模式的构建与应用

介绍基于马斯洛需求层次理论的灾后心理危机干预模式(psychological crisis intervention model based on Maslow’s hierarchy of needs,PCIMM)的概念、结构、常见危机问题与干预措施,以及应用效果。应用研究表明,PCIMM是灾后心理危机干预的一种新理念,能够有效改善危机个体的心理状态、应对能力、生理症状,帮助其摆脱危机状态,提高社会适应能力。

秦-祁-昆造山带元古宙副变质岩层碎屑锆石年龄谱研究

根据秦-祁-昆造山带中北大河岩群、湟源岩群、化隆岩群、金水口岩群、陡岭岩群和秦岭岩群等副变质岩层碎屑锆石年龄谱资料,这些原定为古元古代的地层,至少包括测年样品在内的部分地层的时代应属中元古代或新元古代初期。研究资料还显示上述碎屑岩层具有大量中元古代早期的物源,而古元古代蚀源物质较匮乏。因此,上述副变质岩层显示出相近的碎屑锆石年龄谱特征。

Molecular Characterisation of Two Coxsackievirus B6 Strains from the Tibet Autonomous Region of China

Enteroviruses (EVs) are members of the genus Enteroviruswithin the orderPicornavirales, family Picornaviridae, and consist of 12 species, including EV-A, EV-B, EV-C, and EV-D, which are associated with human infections. Coxsackievirus B6 (CV-B6)belongs to the species EV-B, which currently consists of 63 serotypes, including echovirus (serotypes 1–7,9, 11–21, 24–27, 29–33), coxsackievirus group A (CVA9), coxsackievirus group B (CV-B, serotypes 1–6),the newly identified EVs (serotypes EV-B69, B73–75,B77–88, B93, B97–98, B100–101, B106–107, and B110–113), and the simian enterovirus SA5.

SIZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit

Chlorophylls and carotenoids are essential and bene fi cial substances for both plant and human health.Identifying the regulatory network of these pigments is necessary for improving fruit quality.In a previous study,we identi fi ed an R2R3-MYB transcription factor,SlMYB72,that plays an important role in chlorophyll and carotenoid metabolism in tomato fruit.Here,we demonstrated that the SlMYB72-interacting protein SlZHD17,which belongs to the zinc-fi nger homeodomain transcription factor family,also functions in chlorophyll and carotenoid metabolism.Silencing SIZHD 17 in tomato improved multiple bene fi cial agronomic traits,including dwar fi sm,accelerated fl owering,and earlier fruit harvest.More importantly,downregulating SIZHD17 in fruits resulted in larger chloroplasts and a higher chlorophyll content.Dual-luciferase,yeast one-hybrid and electrophoretic mobility shift assays clari fi ed that SlZHD17 regulates the chlorophyll biosynthesis gene SIPOR-B and chloroplast developmental regulator SITKN2 in a direct manner.Chlorophyll degradation and plastid transformation were also retarded after suppression of SIZHD17 in fruits,which was caused by the inhibition of SISGR1,a crucial factor in chlorophyll degradation.On the other hand,the expression of the carotenoid biosynthesis genes SIPSY1 and SIZISO was also suppressed and directly regulated by SlZHD17,which induced uneven pigmentation and decreased the lycopene content in fruits with SIZHD17 suppression at the ripe stage.Furthermore,the protein-protein interactions between SlZHD17 and other pigment regulators,including SlARF4,SlBEL11,and SlTAGL1,were also presented.This study provides new insight into the complex pigment regulatory network and provides new options for breeding strategies aiming to improve fruit quality.

SCALP ACUPUNCTURE THERAPY AND ITS CLINICAL APPLICATION

Scalp acupuncture belongs to the cate-gory of small-needle therapy and is themethod of treating general diseases by need-ling Shu acupoints in the hair-area scalp.Since 1970s,it has been widely applied,andbecome so far an effective acupuncturetherapy accepted by acupuncturists all overthe world.Clinically,the therapy can beused to treat more than 80 diseases,with ma-rked therapeutic effects particularly on dis-eases of the central nervous system and onvarious acute or chronic pains.

A Reverse-Transcription Recombinase-Aided Amplification Assay for the Rapid Detection of the Wuxiang Virus

Wuxiang virus(WUXV),a new species of Phlebovirus from sandfly specimens(identified as Phlebotomus chinensis)collected in Wuxiang County,Shanxi Province,China,belongs to the order Bunyavirales,family Phenuiviridae.Like other Bunyavirales,WUXV contains a negative-sense tripartite RNA genome comprising a small(S),a medium(M),and a large(L)segment.The S segment encodes nucleocapsid protein(NP)and a nonstructural protein(NSP).The M segment encodes a glycoprotein precursor(Gp),which is cleaved into mature N and C glycoproteins.The L segment encodes an RNA-dependent RNA polymerase(RdRp).

New variola(mpox)in Brazil:Epidemiological update and perspectives

New variola(NV)-mpox(monkeypox)-is a zoonosis caused by the mpox virus,which belongs to the genus Orthopoxvirus in the family Poxviridae.This disease,in the present year(2022),has caused several cases and deaths around the world,including Brazil[1].The group of poxviruses,in which the species that causes NV is included,consists of agents widely distributed in nature.Due to their considerably large size,they were among the first viruses to be microscopically visualized.The Poxviridae family is capable of infecting epithelial and connective tissue cells.Currently,such a viral family is classified as a genome lineage composed of doublestranded DNA,completely independent of the host nucleus[1].

A Novel p.Tyr129His Variant in SIX1 Leads to Dominant,Delayed-onset Hearing Loss with Possible Association with Congenital Anosmia

SIX1(OMIM 601205),as a member of the SIX homeobox transcription factor family,belongs to homologs of the Drosophila‘sine oculis’gene that expresses primarily in the developing visual system of the fly.The SIX family includes six members(SIX1–SIX6),which share a homologous DNA-binding homeodomain(HD)and a highly conserved protein–protein interacting SIX domain(SD)[1].

De novo Assembly of Pen Shell(Atrina pectinata) Transcriptome and Screening of Its Genic Microsatellites

The pen shell(Atrina pectinata) is a large wedge-shaped bivalve, which belongs to family Pinnidae. Due to its large and nutritious adductor muscle, it is the popular seafood with high commercial value in Asia-Pacific countries. However, limiting genomic and transcriptomic data have hampered its genetic investigations. In this study, the transcriptome of A. pectinata was deeply sequenced using Illumina pair-end sequencing technology. After assembling, a total of 127263 unigenes were obtained. Functional annotation indicated that the highest percentage of unigenes(18.60%) was annotated on GO database, followed by 18.44% on PFAM database and 17.04% on NR database. There were 270 biological pathways matched with those in KEGG database. Furthermore, a total of 23452 potential simple sequence repeats(SSRs) were identified, of them the most abundant type was mono-nucleotide repeats(12902, 55.01%), which was followed by di-nucleotide(8132, 34.68%), tri-nucleotide(2010, 8.57%), tetra-nucleotide(401, 1.71%), and penta-nucleotide(7, 0.03%) repeats. Sixty SSRs were selected for validating and developing genic SSR markers, of them 23 showed polymorphism in a cultured population with the average observed and expected heterozygosities of 0.412 and 0.579, respectively. In this study, we established the first comprehensive transcript dataset of A. pectinata genes. Our results demonstrated that RNA-Seq is a fast and cost-effective method for genic SSR development in non-model species.

Nonlinear dynamics of pork price in China

This paper primarily analyzes the evolution path of China＇s pork price by employing the threshold autoregression model（TAR）. Considering the unit root test with a threshold effect and heteroskedasticity of the TAR model, we show that the pork price series is a unit root process in each regime, and the heteroskedasticity in the TAR model greatly affects the results of linearity test. We find that the changing process of pork price has two regimes： mild regime and expansion regime. In particular, a change belongs to an expansion regime if it is larger than 0.5881; otherwise, it falls in the mild regime.