The enhancing effect of benserazide on the chemiluminescence of luminol-potassium ferricyanide in alkaline medium was investigated, and a sensitive method for detecting benserazide has been developed by flow-injection...The enhancing effect of benserazide on the chemiluminescence of luminol-potassium ferricyanide in alkaline medium was investigated, and a sensitive method for detecting benserazide has been developed by flow-injection chemiluminescence＇s. The experimental results showed that the emission intensity is proportional to the concentration of benserazide in the range of 0.01-1.0 μg/mL, The detection limit （S/N=3） for benserazide turned out to be 0.9 ng/mL, and the relative standard deviation for 11 determinations of a 0.10μg/mL benserazide was 2.4%. The method was satisfactorily applied to the determination of benserazide in spiked urine samples.展开更多
A carbon paste electrode modified with carbon nanotubes and ferrocene was fabricated.An electrochemical study of the modified electrode and an investigation into its efficiency for the electrocatalytic oxidation of be...A carbon paste electrode modified with carbon nanotubes and ferrocene was fabricated.An electrochemical study of the modified electrode and an investigation into its efficiency for the electrocatalytic oxidation of benserazide,uric acid and folic acid were undertaken.The electrode was also used to study the electrocatalytic oxidation of benserazide using cyclic voltammetry,chronoamperometry,and square wave voltammetry(SWV).We found that the oxidation of benserazide at the surface of the modified electrode occurs at a potential about 285 mV lower than that of unmodified carbon paste electrode.SWV gave a linear dynamic range from 8.0×10-7 to 7.0×10 4 mol/L.The detection limit was 1.0×10-7 mol/L for benserazide.This modified electrode was used for the determination of benserazide,uric acid,and folic acid in an urine sample.展开更多
We previously created and investigated a planarian model for the study of drug action, abuse, physical dependence, receptor affinity, the toxicity of heavy metals in wastewater, and seizures. For the present pilot stu...We previously created and investigated a planarian model for the study of drug action, abuse, physical dependence, receptor affinity, the toxicity of heavy metals in wastewater, and seizures. For the present pilot study, we investigated the possibility that this model might be useful for studying certain aspects of drugs used in treatment of Parkinson disease. For the first step, we were interested in finding an in vivo metric for the inhibition of L-DOPA by an inhibitor of DOPA decarboxylase. The direct clinical relevance of the endpoint was of secondary concern during this preliminary phase of model development. Two metrics were explored: L-DOPA-induced inhibition of motility (locomotor velocity) and dopamine-mediated toxicity, which was quantified using a Kaplan-Meier survival curve. L-DOPA produced both dose- and time-related toxicity. The water-soluble DOPA decarboxylase inhibitor benserazide dose-dependently inhibited the effect of L-DOPA, as manifested by a leftward shift in the Kaplan-Meier curve. Additional work was initiated using the more sensitive and a graded metric of spontaneous locomotor velocity. The encouraging results of this pilot study suggest that: 1) planarians contain DOPA decarboxylase or an equivalent enzyme, and 2) the planarian model might be useful for the study of certain aspects of anti-Parkinsonism pharmacotherapy.展开更多
基金supported by the Science Foundation of the National Education Department(No.206096)the Education Department of Hubei Province(No.Z200522002)the 0pen Foundation of Hubei Key Laboratory of Biotechnology in Traditional Chinese medicine(No.20040201).
文摘The enhancing effect of benserazide on the chemiluminescence of luminol-potassium ferricyanide in alkaline medium was investigated, and a sensitive method for detecting benserazide has been developed by flow-injection chemiluminescence＇s. The experimental results showed that the emission intensity is proportional to the concentration of benserazide in the range of 0.01-1.0 μg/mL, The detection limit （S/N=3） for benserazide turned out to be 0.9 ng/mL, and the relative standard deviation for 11 determinations of a 0.10μg/mL benserazide was 2.4%. The method was satisfactorily applied to the determination of benserazide in spiked urine samples.
文摘A carbon paste electrode modified with carbon nanotubes and ferrocene was fabricated.An electrochemical study of the modified electrode and an investigation into its efficiency for the electrocatalytic oxidation of benserazide,uric acid and folic acid were undertaken.The electrode was also used to study the electrocatalytic oxidation of benserazide using cyclic voltammetry,chronoamperometry,and square wave voltammetry(SWV).We found that the oxidation of benserazide at the surface of the modified electrode occurs at a potential about 285 mV lower than that of unmodified carbon paste electrode.SWV gave a linear dynamic range from 8.0×10-7 to 7.0×10 4 mol/L.The detection limit was 1.0×10-7 mol/L for benserazide.This modified electrode was used for the determination of benserazide,uric acid,and folic acid in an urine sample.
文摘We previously created and investigated a planarian model for the study of drug action, abuse, physical dependence, receptor affinity, the toxicity of heavy metals in wastewater, and seizures. For the present pilot study, we investigated the possibility that this model might be useful for studying certain aspects of drugs used in treatment of Parkinson disease. For the first step, we were interested in finding an in vivo metric for the inhibition of L-DOPA by an inhibitor of DOPA decarboxylase. The direct clinical relevance of the endpoint was of secondary concern during this preliminary phase of model development. Two metrics were explored: L-DOPA-induced inhibition of motility (locomotor velocity) and dopamine-mediated toxicity, which was quantified using a Kaplan-Meier survival curve. L-DOPA produced both dose- and time-related toxicity. The water-soluble DOPA decarboxylase inhibitor benserazide dose-dependently inhibited the effect of L-DOPA, as manifested by a leftward shift in the Kaplan-Meier curve. Additional work was initiated using the more sensitive and a graded metric of spontaneous locomotor velocity. The encouraging results of this pilot study suggest that: 1) planarians contain DOPA decarboxylase or an equivalent enzyme, and 2) the planarian model might be useful for the study of certain aspects of anti-Parkinsonism pharmacotherapy.
