K i d d 血型系统在国际输血协会( the International Society of Blood Transfusion,ISBT)的命名中符号为JK,数字编码为009。Kidd血型系统有3个抗原:JK^a、JK^b、JK^3。1951年,ALLEN等[1]在Kidd血型的产妇血清中发现了一种新的抗体,被...K i d d 血型系统在国际输血协会( the International Society of Blood Transfusion,ISBT)的命名中符号为JK,数字编码为009。Kidd血型系统有3个抗原:JK^a、JK^b、JK^3。1951年,ALLEN等[1]在Kidd血型的产妇血清中发现了一种新的抗体,被称为抗-JK^a抗体。这种抗体导致该产妇的孩子发生了新生儿溶血症(hemolytic disease of newborn,HDN)。2年后,PLAUT等[2]在1例发生输血反应的妇女血液中发现了对偶抗体,被称为抗-JK^b抗体。抗-JK抗体为免疫性抗体,如患者红细胞上缺乏JK^a或JK^b抗原,因妊娠或输血受到JK^a或JK^b抗原的免疫刺激产生。抗-JK^a抗体和抗-JK^b抗体在中国人群中并不常见,在体内及体外的抗体活性均极易减弱,约有50%的抗-JK抗体能结合补体,绝大部分抗JK-抗体能通过间接抗人球蛋白试验、酶法和聚乙二醇(polyethylene glycol,PEG)法检出,常合并其他血型抗体,有剂量效应[3]。本研究回顾分析1例抗-Ec抗体合并抗-JK^b抗体的病例,以帮助医务人员对此类疾病的了解。展开更多
γ-ECS基因编码的γ-谷氨酰半胱氨酸合成酶是细胞内谷胱甘肽(GSH)从头合成的限速酶。以龙眼转录组数据库为基础,采用同源克隆的方法从龙眼胚性愈伤组织中分离得到γ-ECS基因cDNA全长序列,在GenBank上的登录号为JF815477。γ-ECS基因序...γ-ECS基因编码的γ-谷氨酰半胱氨酸合成酶是细胞内谷胱甘肽(GSH)从头合成的限速酶。以龙眼转录组数据库为基础,采用同源克隆的方法从龙眼胚性愈伤组织中分离得到γ-ECS基因cDNA全长序列,在GenBank上的登录号为JF815477。γ-ECS基因序列全长1 812 bp,包括17 bp 5′UTR及254 bp 3′UTR;ORF长度为1 541 bp编码511个氨基酸。生物信息学分析结果表明,龙眼胚性愈伤组织γ-ECS的核甘酸序列及其推导的氨基酸序列分别与蓖麻、毛果杨等具有较高的同源性;γ-ECS为1个具有跨膜结构的非分泌蛋白,具有亲水性;亚细胞定位于过氧化物酶体,不含信号肽,并存在亮氨酸拉链结构。龙眼胚性愈伤组织γ-ECS基因在龙眼体胚发生过程中的整体表达趋势是先升高,后降低,其表达量在心形胚阶段达到最大值之后在鱼雷形胚阶段急剧下降。γ-ECS基因表达量变化可能与GSH在龙眼体胚发生过程中的含量变化一致。展开更多
The antibody against AT1-EC2 plays a role in some kinds of inflammatory vascular diseases including malignant hypertension,preeclampsia,and renal-allograft rejection,but the detailed mechanisms remain unclear.In order...The antibody against AT1-EC2 plays a role in some kinds of inflammatory vascular diseases including malignant hypertension,preeclampsia,and renal-allograft rejection,but the detailed mechanisms remain unclear.In order to investigate the changes of NADPH oxidase and reactive oxygen species in the aorta in a mouse model which can produce AT1-EC2 antibody by active immunization with AT1-EC2 peptide,15 mice were divided into three groups:control group,AT1-EC2-immunized group,and AT1-EC2-immunized and valsartan-treated group.In AT1-EC2-immunized group and AT1-EC2-immunized and valsartan-treated group,the mice were immunized by 50 μg peptide subcutaneously at multiple points for 4 times:0,5,10,and 15 days after the experiment.In AT1-EC2-immunized and valsartan-treated group,valsartan was given at a dose of 100 mg/kg every day for 20 days.After the experiment,the mice were sacrificed under anesthesia and the aortas were obtained and frozen in liquid nitrogen for the preparation of frozen section slides and other experiments.The titer of AT1-EC2 was assayed by using ELISA.The level of NOX1 mRNA in the aorta was determined by using RT-PCR.The expression of NOX1 was detected by using Western blotting.Confocal scanning microscopy was used to assay the α-actin and NOX1 expression in the aortic tissue.The O 2.production was detected in situ after DHE staining.The mice produced high level antibody against AT1-EC2 in AT1-EC2-immunized group and AT1-EC2-immunized and valsartan-treated group,and the level of NOX1 mRNA in the aortic tissues was 1.6±0.4 times higher and the NOX1 protein expression was higher in AT1-EC2-immunized group than in control group.There were no significant differences in the level of NOX1 mRNA and protein expression between control group and AT1-EC2-immunized and valsartan-treated group.The expression and co-localization of α-actin and NOX1 in AT1-EC2-immunized group increased significantly as compared with those in control group,and the O 2.production increased about 2.7 times as compared with control group.There were no significant differences between control group and AT1-EC2-immunized and valsartan-treated group.It is concluded that active immunization with AT1-EC2 can activate NOX1-ROS,and increase vascular inflammation,which can be inhibited by AT1 receptor blocker valsartan.This may partially explain the mechanism of the pathogenesis of inflammatory vascular diseases related to antibody against AT1-EC2.展开更多
基于顶空固相微萃取(head space solid phase microextraction,HS-SPME)结合全二维气相色谱-飞行时间质谱(comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry,GC×GC-TOFMS)建立了一种准确定量...基于顶空固相微萃取(head space solid phase microextraction,HS-SPME)结合全二维气相色谱-飞行时间质谱(comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry,GC×GC-TOFMS)建立了一种准确定量白酒中氨基甲酸乙酯(ethyl carbamate,EC)的方法。实验对HS-SPME方法进行了优化,在酒精度12%vol、加NaCl 3 g、萃取温度70℃,萃取时间40 min的条件下,能够显著提高目标物的响应值。实验以D_(5)-氨基甲酸乙酯(D_(5)-EC)为内标,对不同浓度的样品溶液进行GC×GC-TOFMS定量分析。结果表明,EC在10~500μg/L范围内线性关系良好,回归系数(R^(2))>0.998,检出限为0.19μg/L,定量限为0.64μg/L,平均回收率为97.57%,精密度为8.66%。测定结果与国标方法相比无显著性差异(P>0.05)。该方法前处理简单,检测灵敏度更高,为白酒中EC自动化定量测定提供了新思路。展开更多
文摘K i d d 血型系统在国际输血协会( the International Society of Blood Transfusion,ISBT)的命名中符号为JK,数字编码为009。Kidd血型系统有3个抗原:JK^a、JK^b、JK^3。1951年,ALLEN等[1]在Kidd血型的产妇血清中发现了一种新的抗体,被称为抗-JK^a抗体。这种抗体导致该产妇的孩子发生了新生儿溶血症(hemolytic disease of newborn,HDN)。2年后,PLAUT等[2]在1例发生输血反应的妇女血液中发现了对偶抗体,被称为抗-JK^b抗体。抗-JK抗体为免疫性抗体,如患者红细胞上缺乏JK^a或JK^b抗原,因妊娠或输血受到JK^a或JK^b抗原的免疫刺激产生。抗-JK^a抗体和抗-JK^b抗体在中国人群中并不常见,在体内及体外的抗体活性均极易减弱,约有50%的抗-JK抗体能结合补体,绝大部分抗JK-抗体能通过间接抗人球蛋白试验、酶法和聚乙二醇(polyethylene glycol,PEG)法检出,常合并其他血型抗体,有剂量效应[3]。本研究回顾分析1例抗-Ec抗体合并抗-JK^b抗体的病例,以帮助医务人员对此类疾病的了解。
文摘γ-ECS基因编码的γ-谷氨酰半胱氨酸合成酶是细胞内谷胱甘肽(GSH)从头合成的限速酶。以龙眼转录组数据库为基础,采用同源克隆的方法从龙眼胚性愈伤组织中分离得到γ-ECS基因cDNA全长序列,在GenBank上的登录号为JF815477。γ-ECS基因序列全长1 812 bp,包括17 bp 5′UTR及254 bp 3′UTR;ORF长度为1 541 bp编码511个氨基酸。生物信息学分析结果表明,龙眼胚性愈伤组织γ-ECS的核甘酸序列及其推导的氨基酸序列分别与蓖麻、毛果杨等具有较高的同源性;γ-ECS为1个具有跨膜结构的非分泌蛋白,具有亲水性;亚细胞定位于过氧化物酶体,不含信号肽,并存在亮氨酸拉链结构。龙眼胚性愈伤组织γ-ECS基因在龙眼体胚发生过程中的整体表达趋势是先升高,后降低,其表达量在心形胚阶段达到最大值之后在鱼雷形胚阶段急剧下降。γ-ECS基因表达量变化可能与GSH在龙眼体胚发生过程中的含量变化一致。
基金supported by National Natural Science Foundation of China (No. 30871069)
文摘The antibody against AT1-EC2 plays a role in some kinds of inflammatory vascular diseases including malignant hypertension,preeclampsia,and renal-allograft rejection,but the detailed mechanisms remain unclear.In order to investigate the changes of NADPH oxidase and reactive oxygen species in the aorta in a mouse model which can produce AT1-EC2 antibody by active immunization with AT1-EC2 peptide,15 mice were divided into three groups:control group,AT1-EC2-immunized group,and AT1-EC2-immunized and valsartan-treated group.In AT1-EC2-immunized group and AT1-EC2-immunized and valsartan-treated group,the mice were immunized by 50 μg peptide subcutaneously at multiple points for 4 times:0,5,10,and 15 days after the experiment.In AT1-EC2-immunized and valsartan-treated group,valsartan was given at a dose of 100 mg/kg every day for 20 days.After the experiment,the mice were sacrificed under anesthesia and the aortas were obtained and frozen in liquid nitrogen for the preparation of frozen section slides and other experiments.The titer of AT1-EC2 was assayed by using ELISA.The level of NOX1 mRNA in the aorta was determined by using RT-PCR.The expression of NOX1 was detected by using Western blotting.Confocal scanning microscopy was used to assay the α-actin and NOX1 expression in the aortic tissue.The O 2.production was detected in situ after DHE staining.The mice produced high level antibody against AT1-EC2 in AT1-EC2-immunized group and AT1-EC2-immunized and valsartan-treated group,and the level of NOX1 mRNA in the aortic tissues was 1.6±0.4 times higher and the NOX1 protein expression was higher in AT1-EC2-immunized group than in control group.There were no significant differences in the level of NOX1 mRNA and protein expression between control group and AT1-EC2-immunized and valsartan-treated group.The expression and co-localization of α-actin and NOX1 in AT1-EC2-immunized group increased significantly as compared with those in control group,and the O 2.production increased about 2.7 times as compared with control group.There were no significant differences between control group and AT1-EC2-immunized and valsartan-treated group.It is concluded that active immunization with AT1-EC2 can activate NOX1-ROS,and increase vascular inflammation,which can be inhibited by AT1 receptor blocker valsartan.This may partially explain the mechanism of the pathogenesis of inflammatory vascular diseases related to antibody against AT1-EC2.
文摘基于顶空固相微萃取(head space solid phase microextraction,HS-SPME)结合全二维气相色谱-飞行时间质谱(comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry,GC×GC-TOFMS)建立了一种准确定量白酒中氨基甲酸乙酯(ethyl carbamate,EC)的方法。实验对HS-SPME方法进行了优化,在酒精度12%vol、加NaCl 3 g、萃取温度70℃,萃取时间40 min的条件下,能够显著提高目标物的响应值。实验以D_(5)-氨基甲酸乙酯(D_(5)-EC)为内标,对不同浓度的样品溶液进行GC×GC-TOFMS定量分析。结果表明,EC在10~500μg/L范围内线性关系良好,回归系数(R^(2))>0.998,检出限为0.19μg/L,定量限为0.64μg/L,平均回收率为97.57%,精密度为8.66%。测定结果与国标方法相比无显著性差异(P>0.05)。该方法前处理简单,检测灵敏度更高,为白酒中EC自动化定量测定提供了新思路。
